Tilapia Lake Virus was not detected in non-tilapine species within tilapia polyculture systems of Bangladesh.

Sixteen countries, including Bangladesh, have reported the presence of tilapia lake virus (TiLV), an emerging tilapia pathogen. Fish polyculture is a common farming practice in Bangladesh. Some unusual mortalities reported in species co-cultivated with TiLV-infected tilapia led us to investigate whether any of the co-cultivated species would also test positive for TiLV and whether they were susceptible to TiLV infection under controlled laboratory experiments. Using 183 samples obtained from 15 farms in six districts across Bangladesh, we determined that 20% of the farms tested positive for TiLV in tilapia, while 15 co-cultivated fish species and seven other invertebrates (e.g. insects and crustaceans) considered potential carriers all tested negative. Of the six representative fish species experimentally infected with TiLV, only Nile tilapia showed the typical clinical signs of the disease, with 70% mortality within 12 days. By contrast, four carp species and one catfish species challenged with TiLV showed no signs of TiLV infection. Challenged tilapia were confirmed as TiLV-positive by RT-qPCR, while challenged carp and walking catfish all tested negative. Overall, our field and laboratory findings indicate that species used in polycultures are not susceptible to TiLV. Although current evidence suggests that TiLV is likely host-specific to tilapia, targeted surveillance for TiLV in other fish species in polyculture systems should continue, in order to prepare for a possible future scenario where TiLV mutates and/or adapts to new host(s).

Synergistic infection of Ichthyophthirius multifiliis and Francisella noatunensis subsp. orientalis in hybrid red tilapia (Oreochromis sp.).

Francisella noatunensis subsp. orientalis (Fno) and Ichthyophthirius multifiliis (Ich) are deadly infectious pathogens in farmed tilapia, particularly during cold season when the water temperature drops to under 25 °C. We hypothesized that infection of the ectoparasite Ich might enhance susceptibility of hybrid red tilapia (Oreochromis sp.) to the facultative intracellular bacterium Fno. To prove the hypothesis, the experiment was designed as follows. Hybrid red tilapia naturally infected by Ich at 9 ± 6 theronts/fish gills and 4 ± 3 theronts/fish skin were distributed into 5 distinct groups exposed to different concentrations of Fno. In parallel, the same number of Ich-free tilapia were challenged to only Fno in the same manner. The results showed that cumulative mortality in the Fno single infection with 2.88 × 106 CFU mL-1 of water was 25 ± 7%, whereas 100% mortality was found in the coinfection treatment at dose of 1.93 × 105 CFU mL-1 of water. No mortality was observed in both control groups (Ich-infected and Ich-free fish). The coinfected fish revealed typical clinical signs and histopathological manifestations of francisellosis and ichthyophthiriasis. This study revealed synergistic effect of the Ich and Fno infection in hybrid red tilapia leading to the exacerbated mortality. Thus, farming management of fish to be free from the Ich ectoparasite might reduce risk of francisellosis and probably other bacterial diseases in farmed tilapia.

Transmission of Francisella noatuensis subsp. orientalis from subclinically infected hybrid red tilapia broodstock (Oreochromis sp.) to their offspring.

Francisella noatunensis subsp. orientalis (Fno) has been reported as an important bacterial pathogen causing significant mortality (30-95%) in farmed tilapia in broad geographic areas. However, we found that there was a proportion of broodfish in our laboratory that appeared to be healthy but which tested positive for Fno. We therefore hypothesized that Fno might be able to be transmitted from subclinically infected tilapia mouthbrooders to their offspring through the current practice of fry production in tilapia hatcheries. To prove this, experimentally infected hybrid red tilapia broodstock were mated and their offspring were examined for the presence of Fno. In this study, three pairs of infected broodfish were mated for natural spawning and fertilized eggs from each couple were then collected from the female mouths for artificial incubation. The newly hatched larvae were cultured for 30 days and sample collection was performed at different developmental stages i.e. yolk-sac larvae, 5 and 30-day old fry. The results showed that the ovary and testis of all 3 pairs of the broodstock, as well as their fertilized eggs and offspring were Fno positive by Fno-specific PCR and in situ DNA hybridization. In summary, this study revealed that with the current practice in tilapia hatcheries, Fno might be able to transmit from subclinically infected tilapia mouthbrooders to their offspring. Therefore, using Fno-free broodfish in tilapia hatcheries should be considered in order to produce Fno-free tilapia fry.