RESUMEN
Sexual dimorphism is common in most vertebrate species and has diverse manifestations. The study of sexual dimorphism has critical significance for evolutionary biological and ecological adaptation. In this study, we analysed the morphometric data of Hynobius maoershanensis, a rare and endangered species, to examine sexual dimorphism in size and shape. A total of 61 H. maoershanensis individuals (9 adult females and 52 adult males) were used in this study. We measured 14 morphological variables and weight of each individual. Analysis of covariance using snout-vent length (SVL) as the covariate showed significant differences in head width (HW), tail length (TL), tail height (TH), forelimb length (FLL), hindlimb length (HLL) and space between axilla and groin (AGS) between the male and female. The female AGS was greater than that of the male, whereas males had greater HW, TL, TH, FLL and HLL than females. The findings show that sexual dimorphism is present in terms of shape but not in terms of size. The wider head of the male could improve mating success, and its thicker limbs and longer tail might facilitate courtship. The females' wider AGS may increase reproductive output. Our results support sexual dimorphism in H. maoershanensis, which could be explained by the sexual selection and fecundity theory hypothesis.
RESUMEN
Intestinal microbiota play an important role in the life of amphibians and its composition may vary by developmental stage. In this study, 16S rRNA high-throughput sequencing was used to profile the intestinal microbiota of Hynobius maoershanensis, which exclusively inhabit the Maoer Mountain swamp at an altitude of approximately 2,000 m. We characterized the bacterial composition, structure, and function of the microbiota of H. maoershanensis at different developmental stages. The alpha diversity was not markedly different for the Simpson, Shannon, Ace, and Sobs indices of microbes. The beta diversity revealed that there were age-related differences in the structure of the intestinal microbes of H. maoershanensis, specifically, at the phylum level. Bacteroidetes and Proteobacteria were the dominant bacteria present in the adult stage, and the relative abundance of Bacteroidetes was significantly higher compared with that of tadpoles. Firmicutes and Proteobacteria were the dominant phylum during the tadpole stage and their relative abundance was significantly higher compared with the adult period. Functional analysis revealed that the pathways associated with organismal systems and metabolism were significantly enriched in the adults, whereas human diseases, genetic information processing, and cellular processes were more enriched in the hindlimb bud stage. Human diseases and environmental information processing were more enriched in the forelimb bud stage at KEGG pathway level 1. Possibilities for the observed discrepancies include the adaptation to eating habits and the remodeling of the intestines during development. We speculated that H. maoershanensis adults may be more suitable to a high-fiber diet, whereas the tadpoles are associated with a carnivorous diet. Our study provides evidence of variations in the intestinal microbiota during development in amphibians, highlighting the influence of historical developments on the intestinal microbiota and an increased understanding of the importance of physiological characteristics in shaping the intestinal microbiota of amphibians. These data will help us formulate more effective protection measures for H. maoershanensis.