Environmental fate and metabolism of the systemic triazolinthione fungicide prothioconazole in different aerobic soils.

As a best-selling triazolinthione fungicide, prothioconazole (PTZ) has been widely used worldwide and has aroused concern about its environmental effect. This study used phenyl-UL-14C-labeled PTZ and an improved fate model to investigate the fate and metabolism of this fungicide in aerobic soil. During 120 d of incubation, PTZ rapidly transformed into metabolites and bound residues, with a half-life (DT50) of less than 1 d. After 120 d, approximately 45-55% of PTZ formed bound residues, and the extractable metabolite residues were gradually degraded over time. Approximately 19%, 44% and 27% of phenyl-UL-14C-PTZ was mineralized in red soil, fluvo-aquic soil and cinnamon soil, respectively, but only approximately 3% was mineralized in black soil. Five metabolites were identified and confirmed, and a possible metabolic pathway for phenyl-UL-14C-PTZ in soil was proposed. Based on the correlation analysis between soil properties and model rate constants, soil properties exerted important effects on PTZ transformation. These results will provide basic data for environmental risk assessments and removal of the PTZ pollutant and suggest that the soil type should be considered in the selection and application of pesticides.

Diverse positional 14C labeling-assisted metabolic analysis of pesticides in rats: The case of vanisulfane, a novel vanillin-derived pesticide.

Information on pesticide metabolites is crucial for accurate environmental risk assessment. However, identifying the various metabolites of a novel pesticide is challenging since the potential metabolic pathways are unknown. In this study, we coupled diverse positional 14C labeling with high-resolution mass spectrometry to quantitatively and qualitatively study pesticide metabolism in rats. With the unique M/(M + 2) ratios derived from 14C, precursor compounds of metabolites could be better distinguished from impurity ions. Additionally, the use of diverse 14C labeling positions is a powerful tool to elucidate the complete metabolic fate of novel contaminants. Vanisulfane is a novel vanillin-derived antiviral agent with encouraging prospects for the efficient control of cucumber mosaic virus in China, but its metabolic pathways in mammals are still poorly understood. Thus, the metabolism of vanisulfane was studied in rats of both sexes by this strategy. The results showed that phase I and phase II metabolism occurred in both sexes. The former included mainly oxidation reactions, and the latter involved binding reactions that formed glucuronide, sulfate and amino acid conjugates. Sex-related differences were observed in the experiment, with earlier appearance of downstream metabolites and a preference for sulfate conjugate formation in males compared to females. This research facilitates the risk evaluation of vanisulfane, and offers an effective framework for screening unknown pesticide metabolic pathways, which could be applied to establish the metabolic profiles of other novel contaminants with limited information.

Review of the genus Daplasa Moore (Lepidoptera, Erebidae, Lymantriinae) from China, with description of new species.

The tussock moth genus Daplasa Moore, 1879 is reviewed from China. Hitherto four species are recognized, where one new species, D. nivisala sp. n., is described and one new combination D. melanoma (Collenette, 1938) comb. n. is proposed. Adults and their genital structures are provided, together with a key to the species of Daplasa with the occurrence in China.