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1.
Cell ; 145(3): 470-82, 2011 Apr 29.
Artículo en Inglés | MEDLINE | ID: mdl-21529718

RESUMEN

High-content screening for gene profiling has generally been limited to single cells. Here, we explore an alternative approach-profiling gene function by analyzing effects of gene knockdowns on the architecture of a complex tissue in a multicellular organism. We profile 554 essential C. elegans genes by imaging gonad architecture and scoring 94 phenotypic features. To generate a reference for evaluating methods for network construction, genes were manually partitioned into 102 phenotypic classes, predicting functions for uncharacterized genes across diverse cellular processes. Using this classification as a benchmark, we developed a robust computational method for constructing gene networks from high-content profiles based on a network context-dependent measure that ranks the significance of links between genes. Our analysis reveals that multi-parametric profiling in a complex tissue yields functional maps with a resolution similar to genetic interaction-based profiling in unicellular eukaryotes-pinpointing subunits of macromolecular complexes and components functioning in common cellular processes.


Asunto(s)
Caenorhabditis elegans/genética , Biología Computacional/métodos , Redes Reguladoras de Genes , Técnicas Genéticas , Animales , Caenorhabditis elegans/embriología , Caenorhabditis elegans/metabolismo , Embrión no Mamífero/metabolismo , Técnicas de Silenciamiento del Gen , Gónadas/embriología , Fenotipo
2.
PLoS Genet ; 18(6): e1010245, 2022 06.
Artículo en Inglés | MEDLINE | ID: mdl-35657999

RESUMEN

LOTUS and Tudor domain containing proteins have critical roles in the germline. Proteins that contain these domains, such as Tejas/Tapas in Drosophila, help localize the Vasa helicase to the germ granules and facilitate piRNA-mediated transposon silencing. The homologous proteins in mammals, TDRD5 and TDRD7, are required during spermiogenesis. Until now, proteins containing both LOTUS and Tudor domains in Caenorhabditis elegans have remained elusive. Here we describe LOTR-1 (D1081.7), which derives its name from its LOTUS and Tudor domains. Interestingly, LOTR-1 docks next to P granules to colocalize with the broadly conserved Z-granule helicase, ZNFX-1. The Tudor domain of LOTR-1 is required for its Z-granule retention. Like znfx-1 mutants, lotr-1 mutants lose small RNAs from the 3' ends of WAGO and mutator targets, reminiscent of the loss of piRNAs from the 3' ends of piRNA precursor transcripts in mouse Tdrd5 mutants. Our work shows that LOTR-1 acts with ZNFX-1 to bring small RNA amplifying mechanisms towards the 3' ends of its RNA templates.


Asunto(s)
Caenorhabditis elegans , Epigénesis Genética , Células Germinativas , Animales , Caenorhabditis elegans/genética , Caenorhabditis elegans/metabolismo , Proteínas de Caenorhabditis elegans , Células Germinativas/metabolismo , ARN Helicasas , ARN Interferente Pequeño/genética , ARN Interferente Pequeño/metabolismo , Dominio Tudor
3.
Cell ; 134(3): 534-45, 2008 Aug 08.
Artículo en Inglés | MEDLINE | ID: mdl-18692475

RESUMEN

Many protein-protein interactions are mediated through independently folding modular domains. Proteome-wide efforts to model protein-protein interaction or "interactome" networks have largely ignored this modular organization of proteins. We developed an experimental strategy to efficiently identify interaction domains and generated a domain-based interactome network for proteins involved in C. elegans early-embryonic cell divisions. Minimal interacting regions were identified for over 200 proteins, providing important information on their domain organization. Furthermore, our approach increased the sensitivity of the two-hybrid system, resulting in a more complete interactome network. This interactome modeling strategy revealed insights into C. elegans centrosome function and is applicable to other biological processes in this and other organisms.


Asunto(s)
Caenorhabditis elegans/embriología , Embrión no Mamífero/metabolismo , Desarrollo Embrionario , Mapeo de Interacción de Proteínas , Animales , División Celular , Dominios y Motivos de Interacción de Proteínas , Proteoma , Técnicas del Sistema de Dos Híbridos
4.
PLoS Genet ; 15(2): e1007905, 2019 02.
Artículo en Inglés | MEDLINE | ID: mdl-30735500

RESUMEN

RNA interference (RNAi) related pathways are essential for germline development and fertility in metazoa and can contribute to inter- and trans-generational inheritance. In the nematode Caenorhabditis elegans, environmental double-stranded RNA provided by feeding can lead to heritable changes in phenotype and gene expression. Notably, transmission efficiency differs between the male and female germline, yet the underlying mechanisms remain elusive. Here we use high-throughput sequencing of dissected gonads to quantify sex-specific endogenous piRNAs, miRNAs and siRNAs in the C. elegans germline and the somatic gonad. We identify genes with exceptionally high levels of secondary 22G RNAs that are associated with low mRNA expression, a signature compatible with silencing. We further demonstrate that contrary to the hermaphrodite germline, the male germline, but not male soma, is resistant to environmental RNAi triggers provided by feeding, in line with previous work. This sex-difference in silencing efficacy is associated with lower levels of gonadal RNAi amplification products. Moreover, this tissue- and sex-specific RNAi resistance is regulated by the germline, since mutant males with a feminized germline are RNAi sensitive. This study provides important sex- and tissue-specific expression data of miRNA, piRNA and siRNA as well as mechanistic insights into sex-differences of gene regulation in response to environmental cues.


Asunto(s)
ARN Interferente Pequeño/genética , Animales , Caenorhabditis elegans/genética , Proteínas de Caenorhabditis elegans/genética , Femenino , Regulación de la Expresión Génica/genética , Células Germinativas/fisiología , Gónadas/fisiología , Secuenciación de Nucleótidos de Alto Rendimiento/métodos , Masculino , MicroARNs/genética , Interferencia de ARN/fisiología , ARN Bicatenario/genética , ARN Mensajero/genética , Caracteres Sexuales
5.
Development ; 143(19): 3540-3548, 2016 10 01.
Artículo en Inglés | MEDLINE | ID: mdl-27510972

RESUMEN

The complex cellular events that occur in response to fertilization are essential for mediating the oocyte-to-embryo transition. Here, we describe a comprehensive small-molecule screen focused on identifying compounds that affect early embryonic events in Caenorhabditis elegans We identify a single novel compound that disrupts early embryogenesis with remarkable stage and species specificity. The compound, named C22, primarily impairs eggshell integrity, leading to osmotic sensitivity and embryonic lethality. The C22-induced phenotype is dependent upon the upregulation of the LET-607/CREBH transcription factor and its candidate target genes, which primarily encode factors involved in diverse aspects of protein trafficking. Together, our data suggest that in the presence of C22, one or more key components of the eggshell are inappropriately processed, leading to permeable, inviable embryos. The remarkable specificity and reversibility of this compound will facilitate further investigation into the role and regulation of protein trafficking in the early embryo, as well as serve as a tool for manipulating the life cycle for other studies such as those involving aging.


Asunto(s)
Proteínas de Caenorhabditis elegans/metabolismo , Caenorhabditis elegans/embriología , Caenorhabditis elegans/metabolismo , Animales , Proteínas de Caenorhabditis elegans/genética , Embrión no Mamífero/metabolismo , Desarrollo Embrionario/genética , Desarrollo Embrionario/fisiología , Oocitos/citología , Oocitos/metabolismo , Factores de Transcripción/genética , Factores de Transcripción/metabolismo
6.
Nature ; 499(7457): 172-7, 2013 Jul 11.
Artículo en Inglés | MEDLINE | ID: mdl-23846655

RESUMEN

RNA-binding proteins are key regulators of gene expression, yet only a small fraction have been functionally characterized. Here we report a systematic analysis of the RNA motifs recognized by RNA-binding proteins, encompassing 205 distinct genes from 24 diverse eukaryotes. The sequence specificities of RNA-binding proteins display deep evolutionary conservation, and the recognition preferences for a large fraction of metazoan RNA-binding proteins can thus be inferred from their RNA-binding domain sequence. The motifs that we identify in vitro correlate well with in vivo RNA-binding data. Moreover, we can associate them with distinct functional roles in diverse types of post-transcriptional regulation, enabling new insights into the functions of RNA-binding proteins both in normal physiology and in human disease. These data provide an unprecedented overview of RNA-binding proteins and their targets, and constitute an invaluable resource for determining post-transcriptional regulatory mechanisms in eukaryotes.


Asunto(s)
Regulación de la Expresión Génica/genética , Motivos de Nucleótidos/genética , Proteínas de Unión al ARN/metabolismo , Trastorno Autístico/genética , Secuencia de Bases , Sitios de Unión/genética , Secuencia Conservada/genética , Células Eucariotas/metabolismo , Humanos , Datos de Secuencia Molecular , Estructura Terciaria de Proteína/genética , Factores de Empalme de ARN , Estabilidad del ARN/genética , Proteínas de Unión al ARN/química , Proteínas de Unión al ARN/genética
7.
PLoS Genet ; 12(6): e1006131, 2016 06.
Artículo en Inglés | MEDLINE | ID: mdl-27341616

RESUMEN

Nucleoporins are the constituents of nuclear pore complexes (NPCs) and are essential regulators of nucleocytoplasmic transport, gene expression and genome stability. The nucleoporin MEL-28/ELYS plays a critical role in post-mitotic NPC reassembly through recruitment of the NUP107-160 subcomplex, and is required for correct segregation of mitotic chromosomes. Here we present a systematic functional and structural analysis of MEL-28 in C. elegans early development and human ELYS in cultured cells. We have identified functional domains responsible for nuclear envelope and kinetochore localization, chromatin binding, mitotic spindle matrix association and chromosome segregation. Surprisingly, we found that perturbations to MEL-28's conserved AT-hook domain do not affect MEL-28 localization although they disrupt MEL-28 function and delay cell cycle progression in a DNA damage checkpoint-dependent manner. Our analyses also uncover a novel meiotic role of MEL-28. Together, these results show that MEL-28 has conserved structural domains that are essential for its fundamental roles in NPC assembly and chromosome segregation.


Asunto(s)
Proteínas de Caenorhabditis elegans/genética , Segregación Cromosómica/genética , Proteínas de Unión al ADN/genética , Proteínas de Complejo Poro Nuclear/genética , Proteínas Nucleares/genética , Transporte Activo de Núcleo Celular/genética , Animales , Caenorhabditis elegans/genética , Ciclo Celular/genética , Línea Celular Tumoral , Cromatina/genética , Células HeLa , Humanos , Células K562 , Membrana Nuclear/genética , Poro Nuclear/genética , Huso Acromático/genética
8.
EMBO J ; 33(16): 1751-66, 2014 Aug 18.
Artículo en Inglés | MEDLINE | ID: mdl-24957527

RESUMEN

The oocyte-to-embryo transition (OET) is thought to be mainly driven by post-transcriptional gene regulation. However, expression of both RNAs and proteins during the OET has not been comprehensively assayed. Furthermore, specific molecular mechanisms that regulate gene expression during OET are largely unknown. Here, we quantify and analyze transcriptome-wide, expression of mRNAs and thousands of proteins in Caenorhabditis elegans oocytes, 1-cell, and 2-cell embryos. This represents a first comprehensive gene expression atlas during the OET in animals. We discovered a first wave of degradation in which thousands of mRNAs are cleared shortly after fertilization. Sequence analysis revealed a statistically highly significant presence of a polyC motif in the 3' untranslated regions of most of these degraded mRNAs. Transgenic reporter assays demonstrated that this polyC motif is required and sufficient for mRNA degradation after fertilization. We show that orthologs of human polyC-binding protein specifically bind this motif. Our data suggest a mechanism in which the polyC motif and binding partners direct degradation of maternal mRNAs. Our data also indicate that endogenous siRNAs but not miRNAs promote mRNA clearance during the OET.


Asunto(s)
Caenorhabditis elegans/embriología , Caenorhabditis elegans/genética , Oocitos/fisiología , Estabilidad del ARN , Regiones no Traducidas 3' , Animales , Animales Modificados Genéticamente , Proteínas de Caenorhabditis elegans/genética , Proteínas de Caenorhabditis elegans/metabolismo , Embrión no Mamífero/citología , Embrión no Mamífero/fisiología , Femenino , Fertilización/genética , Perfilación de la Expresión Génica , Regulación del Desarrollo de la Expresión Génica , MicroARNs , Poli C , Proteoma/metabolismo , ARN Mensajero Almacenado/metabolismo , ARN Interferente Pequeño , Proteínas de Unión al ARN/genética , Proteínas de Unión al ARN/metabolismo
9.
Mol Cell Proteomics ; 15(5): 1642-57, 2016 05.
Artículo en Inglés | MEDLINE | ID: mdl-26912668

RESUMEN

Studying protein interactions in whole organisms is fundamental to understanding development. Here, we combine in vivo expressed GFP-tagged proteins with quantitative proteomics to identify protein-protein interactions of selected key proteins involved in early C. elegans embryogenesis. Co-affinity purification of interaction partners for eight bait proteins resulted in a pilot in vivo interaction map of proteins with a focus on early development. Our network reflects known biology and is highly enriched in functionally relevant interactions. To demonstrate the utility of the map, we looked for new regulators of P granule dynamics and found that GEI-12, a novel binding partner of the DYRK family kinase MBK-2, is a key regulator of P granule formation and germline maintenance. Our data corroborate a recently proposed model in which the phosphorylation state of GEI-12 controls P granule dynamics. In addition, we find that GEI-12 also induces granule formation in mammalian cells, suggesting a common regulatory mechanism in worms and humans. Our results show that in vivo interaction proteomics provides unique insights into animal development.


Asunto(s)
Proteínas de Caenorhabditis elegans/análisis , Caenorhabditis elegans/embriología , Proteínas Portadoras/metabolismo , Proteínas Serina-Treonina Quinasas/metabolismo , Proteínas Tirosina Quinasas/metabolismo , Proteómica/métodos , Animales , Caenorhabditis elegans/metabolismo , Proteínas de Caenorhabditis elegans/metabolismo , Cromatografía de Afinidad , Gránulos Citoplasmáticos/metabolismo , Regulación del Desarrollo de la Expresión Génica , Espectrometría de Masas , Fosforilación , Mapas de Interacción de Proteínas , Quinasas DyrK
10.
Genome Res ; 24(7): 1209-23, 2014 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-24985915

RESUMEN

Accurate gene model annotation of reference genomes is critical for making them useful. The modENCODE project has improved the D. melanogaster genome annotation by using deep and diverse high-throughput data. Since transcriptional activity that has been evolutionarily conserved is likely to have an advantageous function, we have performed large-scale interspecific comparisons to increase confidence in predicted annotations. To support comparative genomics, we filled in divergence gaps in the Drosophila phylogeny by generating draft genomes for eight new species. For comparative transcriptome analysis, we generated mRNA expression profiles on 81 samples from multiple tissues and developmental stages of 15 Drosophila species, and we performed cap analysis of gene expression in D. melanogaster and D. pseudoobscura. We also describe conservation of four distinct core promoter structures composed of combinations of elements at three positions. Overall, each type of genomic feature shows a characteristic divergence rate relative to neutral models, highlighting the value of multispecies alignment in annotating a target genome that should prove useful in the annotation of other high priority genomes, especially human and other mammalian genomes that are rich in noncoding sequences. We report that the vast majority of elements in the annotation are evolutionarily conserved, indicating that the annotation will be an important springboard for functional genetic testing by the Drosophila community.


Asunto(s)
Biología Computacional/métodos , Drosophila melanogaster/genética , Perfilación de la Expresión Génica , Anotación de Secuencia Molecular , Transcriptoma , Animales , Análisis por Conglomerados , Drosophila melanogaster/clasificación , Evolución Molecular , Exones , Femenino , Genoma de los Insectos , Humanos , Masculino , Motivos de Nucleótidos , Filogenia , Posición Específica de Matrices de Puntuación , Regiones Promotoras Genéticas , Edición de ARN , Sitios de Empalme de ARN , Empalme del ARN , Reproducibilidad de los Resultados , Sitio de Iniciación de la Transcripción
11.
Proc Natl Acad Sci U S A ; 108(26): 10508-13, 2011 Jun 28.
Artículo en Inglés | MEDLINE | ID: mdl-21670261

RESUMEN

We present a model of cytoplasmically driven microtubule-based pronuclear motion in the single-celled Caenorhabditis elegans embryo. In this model, a centrosome pair at the male pronucleus initiates stochastic microtubule (MT) growth. These MTs encounter motor proteins, distributed throughout the cytoplasm, that attach and exert a pulling force. The consequent MT-length-dependent pulling forces drag the pronucleus through the cytoplasm. On physical grounds, we assume that the motor proteins also exert equal and opposite forces on the surrounding viscous cytoplasm, here modeled as an incompressible Newtonian fluid constrained within an ellipsoidal eggshell. This naturally leads to streaming flows along the MTs. Our computational method is based on an immersed boundary formulation that allows for the simultaneous treatment of fluid flow and the dynamics of structures immersed within. Our simulations demonstrate that the balance of MT pulling forces and viscous nuclear drag is sufficient to move the pronucleus, while simultaneously generating minus-end directed flows along MTs that are similar to the observed movement of yolk granules toward the center of asters. Our simulations show pronuclear migration, and moreover, a robust pronuclear centration and rotation very similar to that observed in vivo. We find also that the confinement provided by the eggshell significantly affects the internal dynamics of the cytoplasm, increasing by an order of magnitude the forces necessary to translocate and center the pronucleus.


Asunto(s)
Caenorhabditis elegans/embriología , Citoplasma/fisiología , Microtúbulos/fisiología , Animales , Biofisica , Núcleo Celular/fisiología , Centrosoma , Modelos Biológicos
12.
PLoS One ; 19(5): e0303839, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38758765

RESUMEN

The interaction between SARS-CoV-2 non-structural protein Nsp9 and the nanobody 2NSP90 was investigated by NMR spectroscopy using the paramagnetic perturbation methodology PENELOP (Paramagnetic Equilibrium vs Nonequilibrium magnetization Enhancement or LOss Perturbation). The Nsp9 monomer is an essential component of the replication and transcription complex (RTC) that reproduces the viral gRNA for subsequent propagation. Therefore preventing Nsp9 recruitment in RTC would represent an efficient antiviral strategy that could be applied to different coronaviruses, given the Nsp9 relative invariance. The NMR results were consistent with a previous characterization suggesting a 4:4 Nsp9-to-nanobody stoichiometry with the occurrence of two epitope pairs on each of the Nsp9 units that establish the inter-dimer contacts of Nsp9 tetramer. The oligomerization state of Nsp9 was also analyzed by molecular dynamics simulations and both dimers and tetramers resulted plausible. A different distribution of the mapped epitopes on the tetramer surface with respect to the former 4:4 complex could also be possible, as well as different stoichiometries of the Nsp9-nanobody assemblies such as the 2:2 stoichiometry suggested by the recent crystal structure of the Nsp9 complex with 2NSP23 (PDB ID: 8dqu), a nanobody exhibiting essentially the same affinity as 2NSP90. The experimental NMR evidence, however, ruled out the occurrence in liquid state of the relevant Nsp9 conformational change observed in the same crystal structure.


Asunto(s)
Epítopos , Simulación de Dinámica Molecular , SARS-CoV-2 , Anticuerpos de Dominio Único , Proteínas no Estructurales Virales , Proteínas no Estructurales Virales/inmunología , Proteínas no Estructurales Virales/química , Proteínas no Estructurales Virales/metabolismo , Anticuerpos de Dominio Único/química , Anticuerpos de Dominio Único/inmunología , Anticuerpos de Dominio Único/metabolismo , SARS-CoV-2/inmunología , Epítopos/inmunología , Epítopos/química , Humanos , Espectroscopía de Resonancia Magnética , Unión Proteica , Multimerización de Proteína , COVID-19/inmunología , COVID-19/virología , Proteínas de Unión al ARN
13.
Nat Methods ; 6(10): 745-51, 2009 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-19734907

RESUMEN

Caenorhabditis elegans is one of the most prominent model systems for embryogenesis, but collecting many precisely staged embryos has been impractical. Thus, early C. elegans embryogenesis has not been amenable to most high-throughput genomics or biochemistry assays. To overcome this problem, we devised a method to collect staged C. elegans embryos by fluorescence-activated cell sorting (eFACS). In a proof-of-principle experiment, we found that a single eFACS run routinely yielded tens of thousands of almost perfectly staged 1-cell stage embryos. As the earliest embryonic events are driven by posttranscriptional regulation, we combined eFACS with second-generation sequencing to profile the embryonic expression of small, noncoding RNAs. We discovered complex and orchestrated changes in the expression between and within almost all classes of small RNAs, including microRNAs and 26G-RNAs, during embryogenesis.


Asunto(s)
Caenorhabditis elegans/embriología , Caenorhabditis elegans/genética , Embrión no Mamífero/metabolismo , Citometría de Flujo/métodos , ARN Interferente Pequeño/metabolismo , Animales , Caenorhabditis elegans/clasificación
14.
Curr Opin Cell Biol ; 17(1): 3-8, 2005 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-15661512

RESUMEN

In the few short years since its discovery, RNA interference (RNAi) has revolutionized the functional analysis of genomes: both technical and conceptual approaches to the investigation of gene function are being transformed as a result of this new technology. Genome-scale RNAi analyses have already been performed in the model organisms Caenorhabditis elegans (in vivo) and Drosophila melanogaster (in cell lines), ushering in a new era of RNAi-based approaches to probing the inner workings of the cell. The transformation of complex phenotypic data into mineable 'digitized' formats is fostering the emergence of a new area of bioinformatics related to the phenome.


Asunto(s)
Biología/métodos , Técnicas Genéticas , Genoma , Interferencia de ARN , Animales , Caenorhabditis elegans , Biología Celular , Drosophila melanogaster , Humanos , Fenotipo , Programas Informáticos
15.
Dev Cell ; 10(4): 509-20, 2006 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-16580995

RESUMEN

Microtubules of the mitotic spindle are believed to provide positional cues for the assembly of the actin-based contractile ring and the formation of the subsequent cleavage furrow during cytokinesis. In Caenorhabditis elegans, astral microtubules have been thought to inhibit cortical contraction outside the cleavage furrow. Here, we demonstrate by live imaging and RNA interference (RNAi) that astral microtubules play two distinct roles in initiating cleavage furrow formation. In early anaphase, microtubules are required for contractile ring assembly; in late anaphase, microtubules show different cortical behavior and seem to suppress cortical contraction at the poles, as suggested in previous studies. These two distinct phases of microtubule behavior depend on distinct regulatory pathways, one involving the gamma-tubulin complex and the other requiring aurora-A kinase. We propose that temporal and spatial regulation of two distinct phases of astral microtubule behavior is crucial in specifying the position and timing of furrowing.


Asunto(s)
Caenorhabditis elegans/embriología , Citocinesis/fisiología , Microtúbulos/metabolismo , Huso Acromático/fisiología , Anafase/fisiología , Animales , Aurora Quinasa A , Biomarcadores/metabolismo , Caenorhabditis elegans/citología , Caenorhabditis elegans/metabolismo , Proteínas de Caenorhabditis elegans/fisiología , Embrión no Mamífero/citología , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Microtúbulos/fisiología , Mutación , Proteínas Serina-Treonina Quinasas/fisiología , Interferencia de ARN/fisiología , Tubulina (Proteína)/fisiología
16.
Nature ; 436(7052): 861-5, 2005 Aug 11.
Artículo en Inglés | MEDLINE | ID: mdl-16094371

RESUMEN

Although numerous fundamental aspects of development have been uncovered through the study of individual genes and proteins, system-level models are still missing for most developmental processes. The first two cell divisions of Caenorhabditis elegans embryogenesis constitute an ideal test bed for a system-level approach. Early embryogenesis, including processes such as cell division and establishment of cellular polarity, is readily amenable to large-scale functional analysis. A first step toward a system-level understanding is to provide 'first-draft' models both of the molecular assemblies involved and of the functional connections between them. Here we show that such models can be derived from an integrated gene/protein network generated from three different types of functional relationship: protein interaction, expression profiling similarity and phenotypic profiling similarity, as estimated from detailed early embryonic RNA interference phenotypes systematically recorded for hundreds of early embryogenesis genes. The topology of the integrated network suggests that C. elegans early embryogenesis is achieved through coordination of a limited set of molecular machines. We assessed the overall predictive value of such molecular machine models by dynamic localization of ten previously uncharacterized proteins within the living embryo.


Asunto(s)
Caenorhabditis elegans/embriología , Caenorhabditis elegans/metabolismo , Desarrollo Embrionario , Modelos Biológicos , Biología de Sistemas/métodos , Algoritmos , Animales , Caenorhabditis elegans/citología , Caenorhabditis elegans/genética , División Celular , Polaridad Celular , Desarrollo Embrionario/genética , Perfilación de la Expresión Génica , Regulación del Desarrollo de la Expresión Génica , Fenotipo , Unión Proteica , Interferencia de ARN , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo
17.
Elife ; 102021 07 05.
Artículo en Inglés | MEDLINE | ID: mdl-34223818

RESUMEN

We describe MIP-1 and MIP-2, novel paralogous C. elegans germ granule components that interact with the intrinsically disordered MEG-3 protein. These proteins promote P granule condensation, form granules independently of MEG-3 in the postembryonic germ line, and balance each other in regulating P granule growth and localization. MIP-1 and MIP-2 each contain two LOTUS domains and intrinsically disordered regions and form homo- and heterodimers. They bind and anchor the Vasa homolog GLH-1 within P granules and are jointly required for coalescence of MEG-3, GLH-1, and PGL proteins. Animals lacking MIP-1 and MIP-2 show temperature-sensitive embryonic lethality, sterility, and mortal germ lines. Germline phenotypes include defects in stem cell self-renewal, meiotic progression, and gamete differentiation. We propose that these proteins serve as scaffolds and organizing centers for ribonucleoprotein networks within P granules that help recruit and balance essential RNA processing machinery to regulate key developmental transitions in the germ line.


Asunto(s)
Proteínas de Caenorhabditis elegans/metabolismo , Caenorhabditis elegans/metabolismo , Células Germinativas/fisiología , Péptidos y Proteínas de Señalización Intracelular/metabolismo , Animales , Caenorhabditis elegans/embriología , Proteínas de Caenorhabditis elegans/genética , ARN Helicasas DEAD-box/genética , ARN Helicasas DEAD-box/metabolismo , Regulación de la Expresión Génica/fisiología , Péptidos y Proteínas de Señalización Intracelular/genética
18.
Adv Biol (Weinh) ; 5(12): e2101113, 2021 12.
Artículo en Inglés | MEDLINE | ID: mdl-34705339

RESUMEN

Following the entry into the host cell, SARS-CoV-2 replication is mediated by the replication transcription complex (RTC) assembled through a number of nonstructural proteins (Nsps). A monomeric form of Nsp9 is particularly important for RTC assembly and function. In the present study, 136 unique nanobodies targeting Nsp9 are generated. Several nanobodies belonging to different B-cell lineages are expressed, purified, and characterized. Results from immunoassays applied to purified Nsp9 and neat saliva from coronavirus disease (COVID-19) patients show that these nanobodies effectively and specifically recognize both recombinant and endogenous Nsp9. Nuclear magnetic resonance analyses supported by molecular dynamics reveal a composite Nsp9 oligomerization pattern and demonstrate that both nanobodies stabilize the tetrameric form of wild-type Nsp9 also identifying the epitopes on the tetrameric assembly. These results can have important implications in the potential use of these nanobodies to combat viral replication.


Asunto(s)
COVID-19 , Anticuerpos de Dominio Único , Antivirales , Humanos , Espectroscopía de Resonancia Magnética , Proteínas de Unión al ARN , SARS-CoV-2 , Proteínas no Estructurales Virales/genética
19.
Dev Biol ; 335(1): 253-62, 2009 Nov 01.
Artículo en Inglés | MEDLINE | ID: mdl-19643102

RESUMEN

The cell-biological events that guide early-embryonic development occur with great precision within species but can be quite diverse across species. How these cellular processes evolve and which molecular components underlie evolutionary changes is poorly understood. To begin to address these questions, we systematically investigated early embryogenesis, from the one- to the four-cell embryo, in 34 nematode species related to C. elegans. We found 40 cell-biological characters that captured the phenotypic differences between these species. By tracing the evolutionary changes on a molecular phylogeny, we found that these characters evolved multiple times and independently of one another. Strikingly, all these phenotypes are mimicked by single-gene RNAi experiments in C. elegans. We use these comparisons to hypothesize the molecular mechanisms underlying the evolutionary changes. For example, we predict that a cell polarity module was altered during the evolution of the Protorhabditis group and show that PAR-1, a kinase localized asymmetrically in C. elegans early embryos, is symmetrically localized in the one-cell stage of Protorhabditis group species. Our genome-wide approach identifies candidate molecules-and thereby modules-associated with evolutionary changes in cell-biological phenotypes.


Asunto(s)
Evolución Biológica , Desarrollo Embrionario/fisiología , Nematodos , Animales , Caenorhabditis elegans/anatomía & histología , Caenorhabditis elegans/embriología , Caenorhabditis elegans/genética , Nematodos/citología , Nematodos/embriología , Nematodos/genética , Fenotipo , Interferencia de ARN
20.
Curr Biol ; 17(18): 1555-60, 2007 Sep 18.
Artículo en Inglés | MEDLINE | ID: mdl-17869112

RESUMEN

Fertilization triggers egg activation and converts the egg into a developing embryo. The events of this egg-to-embryo transition typically include the resumption of meiosis, the reorganization of the cortical actin cytoskeleton, and the remodeling of the oocyte surface. The factors that regulate sperm-dependent egg-activation events are not well understood. Caenorhabditis elegans EGG-3, a member of the protein tyrosine phosphatase-like (PTPL) family, is essential for regulating cell-surface and cortex rearrangements during egg activation in response to sperm entry. Although fertilization occurred normally in egg-3 mutants, the polarized dispersal of F-actin is altered, a chitin eggshell is not formed, and no polar bodies are produced. EGG-3 is associated with the oocyte plasma membrane in a pattern that is similar to CHS-1 and MBK-2. CHS-1 is required for eggshell deposition, whereas MBK-2 is required for the degradation of maternal proteins during the egg-to-embryo transition. The localization of CHS-1 and EGG-3 are interdependent and both genes were required for the proper localization of MBK-2 in oocytes. Therefore, EGG-3 plays a central role in egg activation by influencing polarized F-actin dynamics and the localization or activity of molecules that are directly involved in executing the egg-to-embryo transition.


Asunto(s)
Proteínas de Caenorhabditis elegans/fisiología , Caenorhabditis elegans/embriología , Óvulo/crecimiento & desarrollo , Proteínas Tirosina Fosfatasas/fisiología , Actinas/análisis , Actinas/metabolismo , Secuencias de Aminoácidos , Animales , Caenorhabditis elegans/genética , Caenorhabditis elegans/metabolismo , Proteínas de Caenorhabditis elegans/análisis , Proteínas de Caenorhabditis elegans/química , Proteínas de Caenorhabditis elegans/metabolismo , Membrana Celular/metabolismo , Embrión no Mamífero/citología , Embrión no Mamífero/metabolismo , Fertilización , Proteínas Fluorescentes Verdes/análisis , Datos de Secuencia Molecular , Óvulo/citología , Óvulo/metabolismo , Proteínas Tirosina Fosfatasas/química , Proteínas Tirosina Fosfatasas/metabolismo , Proteínas Tirosina Quinasas/análisis , Proteínas Tirosina Quinasas/metabolismo
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