Exploring the synergy of enhanced weathering and Bacillus subtilis: A promising strategy for sustainable agriculture.

Climate change is one of the most urgent environmental challenges that humanity faces. In addition to the reduction of greenhouse gas emissions, safe and robust carbon dioxide removal (CDR) technologies that capture atmospheric CO2 and ensure long-term sequestration are required. Among CDR technologies, enhanced silicate weathering (ESW) has been suggested as a promising option. While ESW has been demonstrated to depend strongly on pH, water, and temperature, recent studies suggest that biota may accelerate mineral weathering rates. Bacillus subtilis is a plant growth-promoting rhizobacterium that can facilitate weathering to obtain mineral nutrients. It is a promising agricultural biofertilizer, as it helps plants acquire nutrients and protects them from environmental stresses. Given that croplands are optimal implementation fields for ESW, any synergy between ESW and B. subtilis can hold great potential for further practice. B. subtilis was reported to enhance weathering under laboratory conditions, but there is a lack of data for soil applications. In a soil-mesocosm experiment, we examined the effect of B. subtilis on basalt weathering. B. subtilis-basalt interaction stimulated basalt weathering and increased soil extractable Fe. The combined application displayed higher CDR potential compared to basalt-only application (3.7 vs. 2.3 tons CO2 ha-1) taking solid and liquid cation pools into account. However, the cumulative CO2 efflux decreased by approximately 2 tons CO2 ha-1 with basalt-only treatment, while the combined application did not affect the CO2 efflux. We found limited mobilization of cations to the liquid phase as most were retained in the soil. Additionally, we found substantial mobilization of basalt-originated Mg, Fe, and Al to oxide- and organic-bound soil fractions. We, therefore, conclude that basalt addition showed relatively low inorganic CDR potential but a high capacity for SOM stabilization. The outcomes indicated the importance of weathering rate-GHG emission integration and the high potential of SOM stabilization in ESW studies.

Design and Construction of an Experimental Setup to Enhance Mineral Weathering through the Activity of Soil Organisms.

Enhanced weathering (EW) is an emerging carbon dioxide (CO2) removal technology that can contribute to climate change mitigation. This technology relies on accelerating the natural process of mineral weathering in soils by manipulating the abiotic variables that govern this process, in particular mineral grain size and exposure to acids dissolved in water. EW mainly aims at reducing atmospheric CO2 concentrations by enhancing inorganic carbon sequestration. Until now, knowledge of EW has been mainly gained through experiments that focused on the abiotic variables known for stimulating mineral weathering, thereby neglecting the potential influence of biotic components. While bacteria, fungi, and earthworms are known to increase mineral weathering rates, the use of soil organisms in the context of EW remains underexplored. This protocol describes the design and construction of an experimental setup developed to enhance mineral weathering rates through soil organisms while concurrently controlling abiotic conditions. The setup is designed to maximize weathering rates while maintaining soil organisms' activity. It consists of a large number of columns filled with rock powder and organic material, located in a climate chamber and with water applied via a downflow irrigation system. Columns are placed above a fridge containing jerrycans to collect the leachate. Representative results demonstrate that this setup is suitable to ensure the activity of soil organisms and quantify their effect on inorganic carbon sequestration. Challenges remain in minimizing leachate losses, ensuring homogeneous ventilation through the climate chamber, and avoiding flooding of the columns. With this setup, an innovative and promising approach is proposed to enhance mineral weathering rates through the activity of soil biota and disentangle the effect of biotic and abiotic factors as drivers of EW.