RESUMEN
OBJECTIVE: In this study we have focused on biocompatibility and osteoinductive capacity analysis of self-manufactured single-phase (HAP) and two-phase (HAP and ß-ТСР) bioactive ceramics with various chemical modifications (Fig. 1). RESULTS: We demonstrate a reduction in solubility for all analyzed composite after the treatment with H2O and H2O2, accompanied by an enhancement in adsorption activity. This modification also resulted in an increase in micro- and macroporosity, along with a rise in the open porosity. Adipose-derived mesenchymal stromal cells demonstrated excellent cell adhesion and survival when cultured with these ceramics. Calcium phosphate ceramics (H-500, HT-500, and HT-1 series) stimulated alkaline phosphatase expression, promoted calcium deposition, and enhanced osteopontin expression in ADSCs, independently inducing osteogenesis without additional osteogenic stimuli. These findings underscore the promising potential of HAP-based bioceramics for bone regeneration/reconstruction.
Asunto(s)
Materiales Biocompatibles , Fosfatos de Calcio , Diferenciación Celular , Cerámica , Células Madre Mesenquimatosas , Osteogénesis , Células Madre Mesenquimatosas/efectos de los fármacos , Células Madre Mesenquimatosas/citología , Células Madre Mesenquimatosas/metabolismo , Cerámica/química , Cerámica/farmacología , Osteogénesis/efectos de los fármacos , Fosfatos de Calcio/farmacología , Fosfatos de Calcio/química , Diferenciación Celular/efectos de los fármacos , Humanos , Materiales Biocompatibles/farmacología , Materiales Biocompatibles/química , Adhesión Celular/efectos de los fármacos , Ensayo de Materiales , Supervivencia Celular/efectos de los fármacos , Fosfatasa Alcalina/metabolismo , Osteopontina/metabolismo , Células Cultivadas , PorosidadRESUMEN
ß-Catenin signaling pathway regulates cardiomyocytes proliferation and differentiation, though its involvement in metabolic regulation of cardiomyocytes remains unknown. We used one-day-old mice with cardiac-specific knockout of ß-catenin and neonatal rat ventricular myocytes treated with ß-catenin inhibitor to investigate the role of ß-catenin metabolism regulation in perinatal cardiomyocytes. Transcriptomics of perinatal ß-catenin-ablated hearts revealed a dramatic shift in the expression of genes involved in metabolic processes. Further analysis indicated an inhibition of lipolysis and glycolysis in both in vitro and in vivo models. Finally, we showed that ß-catenin deficiency leads to mitochondria dysfunction via the downregulation of Sirt1/PGC-1α pathway. We conclude that cardiac-specific ß-catenin ablation disrupts the energy substrate shift that is essential for postnatal heart maturation, leading to perinatal lethality of homozygous ß-catenin knockout mice.