Reducing Drought Stress in Plants by Encapsulating Plant Growth-Promoting Bacteria with Polysaccharides.

Drought is a major abiotic stress imposed by climate change that affects crop production and soil microbial functions. Plants respond to water deficits at the morphological, biochemical, and physiological levels, and invoke different adaptation mechanisms to tolerate drought stress. Plant growth-promoting bacteria (PGPB) can help to alleviate drought stress in plants through various strategies, including phytohormone production, the solubilization of mineral nutrients, and the production of 1-aminocyclopropane-1-carboxylate deaminase and osmolytes. However, PGPB populations and functions are influenced by adverse soil factors, such as drought. Therefore, maintaining the viability and stability of PGPB applied to arid soils requires that the PGPB have to be protected by suitable coatings. The encapsulation of PGPB is one of the newest and most efficient techniques for protecting beneficial bacteria against unfavorable soil conditions. Coatings made from polysaccharides, such as sodium alginate, chitosan, starch, cellulose, and their derivatives, can absorb and retain substantial amounts of water in the interstitial sites of their structures, thereby promoting bacterial survival and better plant growth.

Encapsulation of Plant Biocontrol Bacteria with Alginate as a Main Polymer Material.

One of the most favored trends in modern agriculture is biological control. However, many reports show that survival of biocontrol bacteria is poor in host plants. Providing biocontrol agents with protection by encapsulation within external coatings has therefore become a popular idea. Various techniques, including extrusion, spray drying, and emulsion, have been introduced for encapsulation of biocontrol bacteria. One commonly used biopolymer for this type of microencapsulation is alginate, a biopolymer extracted from seaweed. Recent progress has resulted in the production of alginate-based microcapsules that meet key bacterial encapsulation requirements, including biocompatibility, biodegradability, and support of long-term survival and function. However, more studies are needed regarding the effect of encapsulation on protective bacteria and their targeted release in organic crop production systems. Most importantly, the efficacy of alginate use for the encapsulation of biocontrol bacteria in pest and disease management requires further verification. Achieving a new formulation based on biodegradable polymers can have significant effects on increasing the quantity and quality of agricultural products.

Advancing agriculture through bioresource technology: The role of cellulose-based biodegradable mulches.

Agriculture plays a pivotal role in meeting the world's ever-growing food demands. However, traditional agricultural practices often have negative consequences for the environment, such as soil erosion and chemical runoff. Recently, there has been a pressing need for advance agricultural practices. Cellulose-based mulches offer a solution by optimizing agricultural productivity while minimizing harm. These mulches are made from renewable bioresources derived from cellulose-rich materials. Compared to plastic mulches, cellulose-based alternatives show potential in improving nutrient retention, soil health, weed suppression, water conservation, and erosion mitigation. The article investigates the characteristics and application methods of cellulose-based mulches, highlighting their biodegradability, water retention, crop protection, and weed suppression capabilities. It also evaluates their economic feasibility, emphasizing their potential to transform sustainable farming practices. Overall, cellulose-based mulches have the potential to revolutionize agriculture, addressing environmental concerns while optimizing productivity. They represent a significant step toward a more sustainable and resilient agricultural system.

Unveiling Methods to Stimulate Plant Resistance against Pathogens.

Plant diseases caused by pathogens pose significant threats to agricultural productivity and food security worldwide. The traditional approach of relying on chemical pesticides for disease management has proven to be unsustainable, emphasizing the urgent need for sustainable and environmentally friendly alternatives. One promising strategy is to enhance plant resistance against pathogens through various methods. This review aims to unveil and explore effective methods for stimulating plant resistance, transforming vulnerable plants into vigilant defenders against pathogens. We discuss both conventional and innovative approaches, including genetic engineering, induced systemic resistance (ISR), priming, and the use of natural compounds. Furthermore, we analyze the underlying mechanisms involved in these methods, highlighting their potential advantages and limitations. Through an understanding of these methods, scientists and agronomists can develop novel strategies to combat plant diseases effectively while minimizing the environmental impact. Ultimately, this research offers valuable insights into harnessing the plant's innate defense mechanisms and paves the way for sustainable disease management practices in agriculture.

Unveiling the Role of Hydrolytic Enzymes from Soil Biocontrol Bacteria in Sustainable Phytopathogen Management.

BACKGROUND: Phytopathogens, encompassing fungi, bacteria, viruses, and nematodes, pose a significant threat to the agricultural industry by causing substantial economic losses through severe plant diseases. The excessive use of synthetic fungicides to combat phytopathogens has raised environmental and human health concerns. RESULTS: Consequently, there is an increasing demand for safe and environmentally friendly biopesticides to align with consumer preferences for uncontaminated food. One particularly promising alternative to synthetic fungicides involves harnessing biocontrol bacteria that produce extracellular hydrolytic enzymes. These enzymes serve to effectively manage phytopathogens while concurrently fostering sustainable plant protection. Among the pivotal hydrolytic enzymes generated by biocontrol bacteria are chitinase, cellulase, protease, lipase, glucanase, and amylase. These enzymes exert their influence by breaking down the cell wall, proteins, and DNA of phytopathogens, thereby establishing a dependable method of biocontrol. CONCLUSIONS: Recognizing the critical role of these hydrolytic enzymes in sustainable biocontrol, this review seeks to delve into their primary functions, contribution to sustainable plant protection, and mechanisms of action. Through an exploration of the potential presented by biocontrol bacteria and their enzymatic mechanisms, we can discern effective and environmentally conscious strategies for managing phytopathogens in agriculture.

A review of chitosan nanoparticles: Nature's gift for transforming agriculture through smart and effective delivery mechanisms.

Chitosan nanoparticles (CNPs) have emerged as a promising tool in agricultural advancements due to their unique properties including, biocompatability, biodegradability, non-toxicity and remarkable versatility. These inherent properties along with their antimicrobial, antioxidant and eliciting activities enable CNPs to play an important role in increasing agricultural productivity, enhancing nutrient absorption and improving pest management strategies. Furthermore, the nano-formulation of chitosan have the ability to encapsulate various agricultural amendments, enabling the controlled release of pesticides, fertilizers, plant growth promoters and biocontrol agents, thus offering precise and targeted delivery mechanisms for enhanced efficiency. This review provides a comprehensive analysis of the latest research and developments in the use of CNPs for enhancing agricultural practices through smart and effective delivery mechanisms. It discusses the synthesis methods, physicochemical properties, and their role in enhancing seed germination and plant growth, crop protection against biotic and abiotic stresses, improving soil quality and reducing the environmental pollution and delivery of agricultural amendments. Furthermore, the potential environmental benefits and future directions for integrating CNPs into sustainable agricultural systems are explored. This review aims to shed light on the transformative potential of chitosan nanoparticles as nature's gift for revolutionizing agriculture and fostering eco-friendly farming practices.

Macromolecules-based encapsulation of pesticides with carriers: A promising approach for safe and effective delivery.

The global issue of pollution caused by the misuse and indiscriminate application of pesticides has reached critical levels. In this vein, encapsulating pesticides with carriers offers a promising approach that impacts key parameters such as pesticide release kinetics, stability, and biocompatibility, enhancing the safe and effective delivery of agrochemicals. Encapsulated pesticides hold the potential to reduce off-target effects, decrease environmental contamination, and improve overall crop protection. This review highlights the potential benefits and challenges associated with the use of both organic and in-organic carriers in pesticide encapsulation, and the current state of research in this field. Overall, the encapsulation of pesticides with carriers presents a promising approach for the safe and effective delivery of these vital agricultural compounds. By harnessing the advantages of encapsulation, this technique offers a potential solution to mitigate the adverse effects of conventional pesticides and contribute towards sustainable and environmentally conscious farming practices. Further research and development in this field is necessary to optimize the encapsulation process, carrier properties and advance towards sustainable and environmentally friendly pesticide delivery systems.

Chitin-induced disease resistance in plants: A review.

Chitin is composed of N-acetylglucosamine units. Chitin a polysaccharide found in the cell walls of fungi and exoskeletons of insects and crustaceans, can elicit a potent defense response in plants. Through the activation of defense genes, stimulation of defensive compound production, and reinforcement of physical barriers, chitin enhances the plant's ability to defend against pathogens. Chitin-based treatments have shown efficacy against various plant diseases caused by fungal, bacterial, viral, and nematode pathogens, and have been integrated into sustainable agricultural practices. Furthermore, chitin treatments have demonstrated additional benefits, such as promoting plant growth and improving tolerance to abiotic stresses. Further research is necessary to optimize treatment parameters, explore chitin derivatives, and conduct long-term field studies. Continued efforts in these areas will contribute to the development of innovative and sustainable strategies for disease management in agriculture, ultimately leading to improved crop productivity and reduced reliance on chemical pesticides.

Pectin-associated immune responses in plant-microbe interactions: A review.

This review explores the role of pectin, a complex polysaccharide found in the plant cell wall, in mediating immune responses during interactions between plants and microbes. The objectives of this study were to investigate the molecular mechanisms underlying pectin-mediated immune responses and to understand how these interactions shape plant-microbe communication. Pectin acts as a signaling molecule, triggering immune responses such as the production of antimicrobial compounds, reinforcement of the cell wall, and activation of defense-related genes. Pectin functions as a target for pathogen-derived enzymes, enabling successful colonization by certain microbial species. The document discusses the complexity of pectin-based immune signaling networks and their modulation by various factors, including pathogen effectors and host proteins. It also emphasizes the importance of understanding the crosstalk between pectin-mediated immunity and other defense pathways to develop strategies for enhancing plant resistance against diseases. The insights gained from this study have implications for the development of innovative approaches to enhance crop protection and disease management in agriculture. Further investigations into the components and mechanisms involved in pectin-mediated immunity will pave the way for future advancements in plant-microbe interaction research.

Modifying lignin: A promising strategy for plant disease control.

Lignin is a complex polymer found in the cell walls of plants, providing structural support and protection against pathogens. By modifying lignin composition and structure, scientists aim to optimize plant defense responses and increase resistance to pathogens. This can be achieved through various genetic engineering techniques which involve manipulating the genes responsible for lignin synthesis. By either up regulating or down regulating specific genes, researchers can alter the lignin content, composition, or distribution in plant tissues. Reducing lignin content in specific tissues like leaves can improve the effectiveness of defense mechanisms by allowing for better penetration of antimicrobial compounds. Overall, Lignin modification through techniques has shown promising results in enhancing various plants resistance against pathogens. Furthermore, lignin modification can have additional benefits beyond pathogen resistance. It can improve biomass processing for biofuel production by reducing lignin recalcitrance, making the extraction of sugars from cellulose more efficient. The complexity of lignin biosynthesis and its interactions with other plant components make it a challenging target for modification. Additionally, the potential environmental impact and regulatory considerations associated with genetically modified organisms (GMOs) require careful evaluation. Ongoing research aims to further optimize this approach and develop sustainable solutions for crop protection.

A novel biopolymer technique for encapsulation of Bacillus velezensis BV9 into double coating biopolymer made by in alginate and natural gums to biocontrol of wheat take-all disease.

Bacillus velezensis has been known for its high potential in controlling agricultural diseases. Technological advances have opened new perspectives for producing effective formulations by reducing some of the obstacles to their use, such as instability and loss of activity due to exposure to adverse environmental conditions. Encapsulation is one of the new approaches in agricultural science. This research describes discoveries related to processes for the microencapsulation of B. velezensis with natural gums. The efficiency, survival, and controlled release of B. velesensis BV9 encapsulated with alginate mixed with zedo gum, mastic gum, and tragacanth gum were evaluated for this aim. Furthermore, under greenhouse conditions, the encapsulated cells were assessed to control Gaeumannomyces graminis var. tritici in wheat. The results indicated that all tested microcapsules protected >60 % of the bacterial cells. The Alginate-Zedo Gum (Alg-ZG) microcapsules showed a better-controlled release over two months. The greenhouse study indicated that treating wheat plants with Alg-ZG microcapsules was the most efficient treatment, suppressing 100 % of the pathogen. The results indicated that Alg-ZG is the most promising mixture to improve the survivability of B. velezensis BV9. Also, using natural gums and great potential of this formulation provides an effective and affordable fertilizers for agriculture.

Advancements in coating technologies: Unveiling the potential of chitosan for the preservation of fruits and vegetables.

Post-harvest losses of fruits and vegetables pose a significant challenge to the agriculture industry worldwide. To address this issue, researchers have turned to natural and eco-friendly solutions such as chitosan coatings. Chitosan, a biopolymer derived from chitin, has gained considerable attention due to its unique properties such as non-toxicity, biodegradability, biocompatibility and potential applications in post-harvest preservation. This review article provides an in-depth analysis of the current state of research on chitosan coatings for the preservation of fruits and vegetables. Moreover, it highlights the advantages of using chitosan coatings, including its antimicrobial, antifungal, and antioxidant properties, as well as its ability to enhance shelf-life and maintain the quality attributes of fresh product. Furthermore, the review discusses the mechanisms by which chitosan interacts with fruits and vegetables, elucidating its antimicrobial activity, modified gas permeability, enhanced physical barrier and induction of host defense responses. It also examines the factors influencing the effectiveness of chitosan coatings, such as concentration, molecular weight, deacetylation degree, pH, temperature, and application methods.

Nano/Micro-Structural Supramolecular Biopolymers: Innovative Networks with the Boundless Potential in Sustainable Agriculture.

Sustainable agriculture plays a crucial role in meeting the growing global demand for food while minimizing adverse environmental impacts from the overuse of synthetic pesticides and conventional fertilizers. In this context, renewable biopolymers being more sustainable offer a viable solution to improve agricultural sustainability and production. Nano/micro-structural supramolecular biopolymers are among these innovative biopolymers that are much sought after for their unique features. These biomaterials have complex hierarchical structures, great stability, adjustable mechanical strength, stimuli-responsiveness, and self-healing attributes. Functional molecules may be added to their flexible structure, for enabling novel agricultural uses. This overview scrutinizes how nano/micro-structural supramolecular biopolymers may radically alter farming practices and solve lingering problems in agricultural sector namely improve agricultural production, soil health, and resource efficiency. Controlled bioactive ingredient released from biopolymers allows the tailored administration of agrochemicals, bioactive agents, and biostimulators as they enhance nutrient absorption, moisture retention, and root growth. Nano/micro-structural supramolecular biopolymers may protect crops by appending antimicrobials and biosensing entities while their eco-friendliness supports sustainable agriculture. Despite their potential, further studies are warranted to understand and optimize their usage in agricultural domain. This effort seeks to bridge the knowledge gap by investigating their applications, challenges, and future prospects in the agricultural sector. Through experimental investigations and theoretical modeling, this overview aims to provide valuable insights into the practical implementation and optimization of supramolecular biopolymers in sustainable agriculture, ultimately contributing to the development of innovative and eco-friendly solutions to enhance agricultural productivity while minimizing environmental impact.

Micro-/Nano-Carboxymethyl Cellulose as a Promising Biopolymer with Prospects in the Agriculture Sector: A Review.

The increase in the population rate has increased the demand for safe and quality food products. However, the current agricultural system faces many challenges in producing vegetables and fruits. Indiscriminate use of pesticides and fertilizers, deficiency of water resources, short shelf life of products postharvest, and nontargeted delivery of agrochemicals are the main challenges. In this regard, carboxymethyl cellulose (CMC) is one of the most promising materials in the agriculture sector for minimizing these challenges due to its mechanical strength, viscosity, wide availability, and edibility properties. CMC also has high water absorbency; therefore, it can be used for water deficiency (as superabsorbent hydrogels). Due to the many hydroxyl groups on its surface, this substance has high efficacy in removing pollutants, such as pesticides and heavy metals. Enriching CMC coatings with additional substances, such as antimicrobial, antibrowning, antioxidant, and antisoftening materials, can provide further novel formulations with unique advantages. In addition, the encapsulation of bioactive materials or pesticides provides a targeted delivery system. This review presents a comprehensive overview of the use of CMC in agriculture and its applications for preserving fruit and vegetable quality, remediating agricultural pollution, preserving water sources, and encapsulating bioactive molecules for targeted delivery.

Encapsulating biocontrol bacteria with starch as a safe and edible biopolymer to alleviate plant diseases: A review.

Healthy foods with few artificial additives are in high demand among consumers. Preserving conventional pesticides, frequently used as chemicals to control phytopathogens, is challenging. Therefore, we proposed an innovative approach to protect agricultural products in this review. Biocontrol bacteria are safe alternatives with low stability and low efficiency in the free-form formulation. The encapsulation technique for covering active compounds (e.g., antimicrobials) represents a more efficient protection technology because encapsulation causes the controlled release of bioactive materials and reduces the application doses. Of the biopolymers able to form a capsule, starch exhibits several advantages, such as its ready availability, cost-effectively, edible, colorless, and tasteless. Nevertheless, the poor mechanical properties of starch can be improved with other edible biopolymers. In addition, applying formulations incorporated with more than one antimicrobial material offers synergistic effects. This review presented the starch-based capsules used to enclose antimicrobial agents as effective tools against phytopathogens.

Chitosan-based nanocomposites as coatings and packaging materials for the postharvest improvement of agricultural product: A review.

The perishability nature of harvested fruits and vegetables, along with the effect of environmental factors, storage conditions, and transportation, reduce the products' quality and shelf-life. Considerable efforts have been allocated to alternate conventional coatings based on new edible biopolymers for packaging. Chitosan is an attractive alternative to synthetic plastic polymers due to its biodegradability, antimicrobial activity, and film-forming properties. However, its conservative properties can be improved by adding active compounds, limiting microbial agents' growth and biochemical and physical damages, and enhancing the stored products' quality, shelf-life, and consumer acceptability. Most of the research on chitosan-based coatings focuses on antimicrobial or antioxidant properties. Along with the advancement of polymer science and nanotechnology, novel chitosan blends with multiple functionalities are required and should be fabricated using numerous strategies, especially for application during storage. This review discusses recent developments in using chitosan as a matrix to fabricate bioactive edible coatings and their positive impacts on increasing the quality and shelf-life of fruits and vegetables.

Chitosan/silica: A hybrid formulation to mitigate phytopathogens.

Long-term and indiscriminate use of synthetic pesticides to mitigate plant pathogens have created serious issues of water health, soil contamination, non-target organisms, resistant species, and unpredictable environmental and human health hazards. These constraints have forced scientists to develop alternative plant disease management strategies to reduce synthetic chemical' dependency. During the last 20 years, biological agents and resistance elicitors have been the most important used alternatives. Silica-based materials/chitosan with a dual mode of action have been proposed as promising alternatives to prevent plant diseases through direct and indirect mechanisms. Moreover, the combined application of nano-silica and chitosan, due to their controllable morphology, high loading capacity, low toxicity, and efficient encapsulation, act as suitable carriers for biological agents, pesticides, and essential oils, making them proper candidates for mitigation of phytopathogens. Based on this potential, this literature study reviewed the silica and chitosan properties and their function in the plant. It also assessed their role in the fighting against soil and aerial phytopathogens, directly and indirectly, as novel hybrid formulations in future managing platforms.

Increasing the efficiency of agricultural fertilizers using cellulose nanofibrils: A review.

Developing new agricultural products, such as new fertilizers with high use efficiency and less negative impact on the environment, is required in sustainable agriculture. In this vein, controlled-release fertilizers (CRFs) have been designed to decrease nutrient waste and increase nutrients' availability to plants. Various CRFs have been developed based on petroleum-derived polymers with many advantages over conventional fertilizers. Although, their use is limited due to their adverse effects on the soil and environment. To overcome these issues, CRFs based on biopolymers represent a new generation of fertilizers developed by encapsulating nutrients with cellulose nanofibrils (CNFs). CNFs and the hydrogels based on CNFs have great potential to be applied as CRFs matrix as they are biodegradable, minimize environmental pollution, and exhibit a great controlled-release potential and water/nutrient retention capacity. In order to gain a better understanding of the potential benefits of these new fertilizers in agricultural systems, this review summarizes the recent advances in CNFs in CRFs, the coating methods, hydrogel preparation techniques, and their impact on plant growth and soil. By examining these factors in depth, a better understanding can be gained on how these novel fertilizers can help improve agricultural productivity and sustainability.

Use of whey protein as a natural polymer for the encapsulation of plant biocontrol bacteria: A review.

Climate changes, drought, the salinity of water and soil, the emergence of new breeds of pests and pathogens, the industrialization of countries, and environmental contamination are among the factors limiting the production of agricultural products. The use of chemicals (in the form of fertilizers, pesticides and fungicides) to enhance products against biotic and abiotic stresses has limitations. To eliminate the effects of agricultural chemicals, synthetic agrochemicals should be replaced with natural substances and useful microorganisms. To be more effective and efficient, plant biocontrol bacteria need a coating layer around themselves to protect them from adverse conditions. Whey protein, a valuable by-product of the cheese industry, is one of the important natural polymers. Due to its high protein content, safety, and biodegradability, whey can have many applications in agriculture and encapsulation of bacteria to resist pests and plant diseases. This compound is a rich source of amino acids that can activate plant defense systems and defense enzymes. Considering the amazing potentialities of formulation whey protein, this review attends to the efficiency of whey protein as coating layers on fruit and vegetables and in the packaging system to increase the shelf life of agricultural products against phytopathogens.

Nano/micro-cellulose-based materials as remarkable sorbents for the remediation of agricultural resources from chemical pollutants.

Overusing pesticides, fertilizers, and synthetic dyes has significantly increased their presence in various parts of the environment. The transportation of these pollutants into agricultural soil and water through rivers, soils, and groundwater has seriously threatened human and ecosystem health. Applying techniques and materials to clean up agricultural sources from pesticides, heavy metals (HMs), and synthetic dyes (SDs) is one of the major challenges in this century. The sorption technique offers a viable solution to remediate these chemical pollutants (CHPs). Cellulose-based materials have become popular in nano and micro scales because they are widely available, safe to use, biodegradable, and have a significant ability to absorb substances. Nanoscale cellulose-based materials exhibit greater capacity in absorbing pollutants compared to their microscale counterparts because they possess a larger surface area. Many available hydroxyl groups (-OH) and chemical and physical modifications enable the incorporation of CHPs on to cellulose-based materials. Following this potential, this review aims to comprehensively summarize recent advancements in the field of nano- and micro-cellulose-based materials as effective adsorbents for CHPs, given the abundance of cellulosic waste materials from agricultural residues. The recent developments pertaining to the enhancement of the sorption capacity of cellulose-based materials against pesticides, HMs, and SDs, are deliberated.