Helminth infection in wild boars in Primorye, Russia.

Wild boars have a worldwide distribution and also have major economic, veterinary, and medical importance. Due to a small amount of data on the parasitic fauna of wild boars in the Russian Far East, especially in Prymorye territory, a post-mortem parasitic examination of 20 wild boars was provided. The general prevalence was 25%, and a total of six helminth species, including one larva stage, were found. The most prevalent helminth species were Gnathostoma doloresi (25%) and Metastrongylus elongatus (20%). Followed by Trichuris suis and Ascaris suum (15%). The lowest prevalence was registered for Cysticercus tenuicollis (the larvae stage of T. hydatigena). Parasites were found in each region, with the highest prevalence registered in the Chuguevskii region. Totally 100% of adult boars were positive for helminth infections, instead of 60% of young specimens. Some helminthes found (G. doloresi, T. suis, A. suum) have zoonotic potential and can be involved in the circulation of human parasitic diseases, especially in rural areas. Further research work on parasitic infections in wild boars in Prymorye is necessary due to the high prevalence of helminthes in wild boars.

Origins, phylogenetic relationships and host-parasite interactions of Troglotrematoidea since the cretaceous.

In the current study, we raise the issue concerning origins and historical relationships of the trematodes from the families Troglotrematidae and Paragonimidae using phylogenetic analysis and molecular-clock method for estimating evolutionary rates. For the first time we provided 28S rRNA gene fragment (1764 bp) for the type species Troglotrema acutum - zoonotic trematodes that cause cranial lesions (troglotremiasis) in mustelid and canid mammals of the Central Europe, Iberian Peninsula, and North-West Caucasus. Molecular genetic analysis revealed that T. acutum belongs to the monophyletic family Troglotrematidae sister with the family Paragonimidae. The family Troglotrematidae includes five genera: Nanophyetus, Troglotrema, Skrjabinophyetus, Nephrotrema, and Macroorchis; and the family Paragonimidae is monotypic including the only genus Paragonimus. We recover the superfamily Troglotrematoidea for these two families. Divergence of the common ancestor of the superfamily Troglotrematoidea (common troglotrematoid ancestor) likely occurred during the Cretaceous period of the Mesozoic Era and potentially originated in the Asiatic region. The lineage of the family Troglotrematidae is much closer to the common troglotrematoid ancestor than the species of the family Paragonimidae. The radiation time of the common troglotrematoid ancestor (126 Ma, the Early Cretaceous), and formation of the families Troglotrematidae and Paragonimidae (96 Ma and 73 Ma respectively, the Late Cretaceous) corresponds to the time of settling in East Asia by many species of mammaliaforms (about 130-70 Ma).

Sequencing and genetic characterization of two strains Paramushir virus obtained from the Tyuleniy Island in the Okhotsk Sea (2015).

Paramushir virus belongs to Sakhalin virus genogroup within Orthonairovirus genus and is one of the poorly studied viruses with unknown pathogenicity. At the moment, only one nearly complete sequence of Paramushir virus genome, isolated in 1972, is available. Two new strains of PARV were isolated in 2015 from a sample collected at the Tyuleniy Island in the Okhotsk Sea and sequenced using a combination of high throughput sequencing and specific multiplex PCR. Both strains are closely related to the early sequenced PARV strain LEIV-1149 K. The signs of intersegment reassortment and probable recombination were revealed, which point to a high variability potential of Paramushir virus and may lead to the formation of strains with novel properties, different from those of the predecessors. The new data regarding Paramushir virus can promote a better understanding of the diversity and relations within Orthonairovirus genus and help define intragenic demarcation criteria, which have not yet been established.