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1.
Immunity ; 31(5): 799-810, 2009 Nov 20.
Artículo en Inglés | MEDLINE | ID: mdl-19853481

RESUMEN

The importance of T helper type 1 (Th1) cell immunity in host resistance to the intracellular bacterium Francisella tularensis is well established. However, the relative roles of interleukin (IL)-12-Th1 and IL-23-Th17 cell responses in immunity to F. tularensis have not been studied. The IL-23-Th17 cell pathway is critical for protective immunity against extracellular bacterial infections. In contrast, the IL-23-Th17 cell pathway is dispensable for protection against intracellular pathogens such as Mycobacteria. Here we show that the IL-23-Th17 pathway regulates the IL-12-Th1 cell pathway and was required for protective immunity against F.tularensis live vaccine strain. We show that IL-17A, but not IL-17F or IL-22, induced IL-12 production in dendritic cells and mediated Th1 responses. Furthermore, we show that IL-17A also induced IL-12 and interferon-gamma production in macrophages and mediated bacterial killing. Together, these findings illustrate a biological function for IL-17A in regulating IL-12-Th1 cell immunity and host responses to an intracellular pathogen.


Asunto(s)
Francisella tularensis , Interleucina-17/metabolismo , Interleucina-23/metabolismo , Células TH1/inmunología , Tularemia/inmunología , Tularemia/prevención & control , Animales , Células Dendríticas/inmunología , Francisella tularensis/inmunología , Interferón gamma/metabolismo , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Transducción de Señal
2.
PLoS Pathog ; 10(5): e1004099, 2014 May.
Artículo en Inglés | MEDLINE | ID: mdl-24831696

RESUMEN

Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis (TB), infects one third of the world's population. Among these infections, clinical isolates belonging to the W-Beijing appear to be emerging, representing about 50% of Mtb isolates in East Asia, and about 13% of all Mtb isolates worldwide. In animal models, infection with W-Beijing strain, Mtb HN878, is considered "hypervirulent" as it results in increased mortality and causes exacerbated immunopathology in infected animals. We had previously shown the Interleukin (IL) -17 pathway is dispensable for primary immunity against infection with the lab adapted Mtb H37Rv strain. However, it is not known whether IL-17 has any role to play in protective immunity against infection with clinical Mtb isolates. We report here that lab adapted Mtb strains, such as H37Rv, or less virulent Mtb clinical isolates, such as Mtb CDC1551, do not require IL-17 for protective immunity against infection while infection with Mtb HN878 requires IL-17 for early protective immunity. Unexpectedly, Mtb HN878 induces robust production of IL-1ß through a TLR-2-dependent mechanism, which supports potent IL-17 responses. We also show that the role for IL-17 in mediating protective immunity against Mtb HN878 is through IL-17 Receptor signaling in non-hematopoietic cells, mediating the induction of the chemokine, CXCL-13, which is required for localization of T cells within lung lymphoid follicles. Correct T cell localization within lymphoid follicles in the lung is required for maximal macrophage activation and Mtb control. Since IL-17 has a critical role in vaccine-induced immunity against TB, our results have far reaching implications for the design of vaccines and therapies to prevent and treat emerging Mtb strains. In addition, our data changes the existing paradigm that IL-17 is dispensable for primary immunity against Mtb infection, and instead suggests a differential role for IL-17 in early protective immunity against emerging Mtb strains.


Asunto(s)
Inmunidad Innata/genética , Interleucina-17/fisiología , Mycobacterium tuberculosis/inmunología , Animales , Células Cultivadas , Enfermedades Transmisibles Emergentes/genética , Enfermedades Transmisibles Emergentes/inmunología , Citoprotección/genética , Citoprotección/inmunología , Femenino , Interleucina-17/genética , Interleucina-1beta/fisiología , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Mycobacterium tuberculosis/patogenicidad , Receptores Tipo I de Interleucina-1/genética , Receptor Toll-Like 2/fisiología , Tuberculosis/genética , Tuberculosis/inmunología
3.
Am J Pathol ; 183(5): 1397-1404, 2013 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-24007881

RESUMEN

IL-10 production during intracellular bacterial infections is generally thought to be detrimental because of its role in suppressing protective T-helper cell 1 (Th1) responses. Francisella tularensis is a facultative intracellular bacterium that activates both Th1 and Th17 protective immune responses. Herein, we report that IL-10-deficient mice (Il10(-/-)), despite having increased Th1 and Th17 responses, exhibit increased mortality after pulmonary infection with F. tularensis live vaccine strain. We demonstrate that the increased mortality observed in Il10(-/-)-infected mice is due to exacerbated IL-17 production that causes increased neutrophil recruitment and associated lung pathology. Thus, although IL-17 is required for protective immunity against pulmonary infection with F. tularensis live vaccine strain, its production is tightly regulated by IL-10 to generate efficient induction of protective immunity without mediating pathology. These data suggest a critical role for IL-10 in maintaining the delicate balance between host immunity and pathology during pulmonary infection with F. tularensis live vaccine strain.


Asunto(s)
Vacunas Bacterianas/inmunología , Francisella tularensis/fisiología , Interleucina-10/metabolismo , Interleucina-17/metabolismo , Pulmón/inmunología , Pulmón/patología , Infecciones del Sistema Respiratorio/microbiología , Animales , Susceptibilidad a Enfermedades , Inflamación/patología , Interleucina-10/biosíntesis , Interleucina-10/deficiencia , Pulmón/microbiología , Ratones , Ratones Endogámicos C57BL , Infiltración Neutrófila , Infecciones del Sistema Respiratorio/inmunología , Infecciones del Sistema Respiratorio/patología , Infecciones del Sistema Respiratorio/prevención & control , Células TH1/inmunología , Células Th17/inmunología , Tularemia/inmunología , Tularemia/microbiología , Tularemia/patología , Tularemia/prevención & control
4.
Am J Respir Crit Care Med ; 188(9): 1137-46, 2013 Nov 01.
Artículo en Inglés | MEDLINE | ID: mdl-24047412

RESUMEN

RATIONALE: A hallmark of pulmonary tuberculosis (TB) is the formation of granulomas. However, the immune factors that drive the formation of a protective granuloma during latent TB, and the factors that drive the formation of inflammatory granulomas during active TB, are not well defined. OBJECTIVES: The objective of this study was to identify the underlying immune mechanisms involved in formation of inflammatory granulomas seen during active TB. METHODS: The immune mediators involved in inflammatory granuloma formation during TB were assessed using human samples and experimental models of Mycobacterium tuberculosis infection, using molecular and immunologic techniques. MEASUREMENTS AND MAIN RESULTS: We demonstrate that in human patients with active TB and in nonhuman primate models of M. tuberculosis infection, neutrophils producing S100 proteins are dominant within the inflammatory lung granulomas seen during active TB. Using the mouse model of TB, we demonstrate that the exacerbated lung inflammation seen as a result of neutrophilic accumulation is dependent on S100A8/A9 proteins. S100A8/A9 proteins promote neutrophil accumulation by inducing production of proinflammatory chemokines and cytokines, and influencing leukocyte trafficking. Importantly, serum levels of S100A8/A9 proteins along with neutrophil-associated chemokines, such as keratinocyte chemoattractant, can be used as potential surrogate biomarkers to assess lung inflammation and disease severity in human TB. CONCLUSIONS: Our results thus show a major pathologic role for S100A8/A9 proteins in mediating neutrophil accumulation and inflammation associated with TB. Thus, targeting specific molecules, such as S100A8/A9 proteins, has the potential to decrease lung tissue damage without impacting protective immunity against TB.


Asunto(s)
Calgranulina A/inmunología , Calgranulina B/inmunología , Granuloma del Sistema Respiratorio/inmunología , Mediadores de Inflamación/inmunología , Neutrófilos/inmunología , Tuberculosis Pulmonar/inmunología , Animales , Quimiocinas/inmunología , Factores Quimiotácticos/inmunología , Citocinas/inmunología , Modelos Animales de Enfermedad , Humanos , Macaca mulatta , Ratones , Ratones Endogámicos C57BL
5.
Eur J Immunol ; 42(2): 364-73, 2012 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-22101830

RESUMEN

The generation of effective type 1 T helper (Th1)-cell responses is required for immunity against intracellular bacteria. However, some intracellular bacteria require interleukin (IL)-17 to drive Th1-cell immunity and subsequent protective host immunity. Here, in a model of Mycobacterium bovis Bacille Calmette-Guerin (BCG) vaccination in mice, we demonstrate that the dependence on IL-17 to drive Th1-cell responses is a host mechanism to overcome bacteria-induced IL-10 inhibitory effects. We show that BCG-induced prostaglandin-E2 (PGE2) promotes the production of IL-10 which limits Th1-cell responses, while simultaneously inducing IL-23 and Th17-cell differentiation. The ability of IL-17 to downregulate IL-10 and induce IL-12 production allows the generation of subsequent Th1-cell responses. Accordingly, BCG-induced Th17-cell responses precede the generation of Th1-cell responses in vivo, whereas the absence of the IL-23 pathway decreases BCG vaccine-induced Th17 and Th1-cell immunity and subsequent vaccine-induced protection upon M. tuberculosis challenge. Importantly, in the absence of IL-10, BCG-induced Th1-cell responses occur in an IL-17-independent manner. These novel data demonstrate a role for the IL-23/IL-17 pathway in driving Th1-cell responses, specifically to overcome IL-10-mediated inhibition and, furthermore, show that in the absence of IL-10, the generation of BCG-induced Th1-cell immunity is IL-17 independent.


Asunto(s)
Interferón gamma/metabolismo , Interleucina-17/metabolismo , Mycobacterium bovis/inmunología , Células TH1/metabolismo , Tuberculosis/inmunología , Animales , Carga Bacteriana/genética , Dinoprostona/inmunología , Dinoprostona/metabolismo , Modelos Animales de Enfermedad , Humanos , Inmunomodulación , Interferón gamma/inmunología , Interleucina-10/genética , Interleucina-17/genética , Interleucina-17/inmunología , Interleucina-23/inmunología , Interleucina-23/metabolismo , Activación de Linfocitos/genética , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Ratones Transgénicos , Mycobacterium bovis/crecimiento & desarrollo , Mycobacterium bovis/patogenicidad , Comunicación Paracrina/genética , Células TH1/inmunología , Células TH1/patología , Vacunación
6.
J Immunol ; 186(3): 1666-1674, 2011 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-21178015

RESUMEN

Staphylococcus aureus is a significant cause of hospital and community acquired pneumonia and causes secondary infection after influenza A. Recently, patients with hyper-IgE syndrome, who often present with S. aureus infections of the lung and skin, were found to have mutations in STAT3, required for Th17 immunity, suggesting a potential critical role for Th17 cells in S. aureus pneumonia. Indeed, IL-17R(-/-) and IL-22(-/-) mice displayed impaired bacterial clearance of S. aureus compared with that of wild-type mice. Mice challenged with influenza A PR/8/34 H1N1 and subsequently with S. aureus had increased inflammation and decreased clearance of both virus and bacteria. Coinfection resulted in greater type I and II IFN production in the lung compared with that with virus infection alone. Importantly, influenza A coinfection resulted in substantially decreased IL-17, IL-22, and IL-23 production after S. aureus infection. The decrease in S. aureus-induced IL-17, IL-22, and IL-23 was independent of type II IFN but required type I IFN production in influenza A-infected mice. Furthermore, overexpression of IL-23 in influenza A, S. aureus-coinfected mice rescued the induction of IL-17 and IL-22 and markedly improved bacterial clearance. These data indicate a novel mechanism by which influenza A-induced type I IFNs inhibit Th17 immunity and increase susceptibility to secondary bacterial pneumonia.


Asunto(s)
Subtipo H1N1 del Virus de la Influenza A/inmunología , Interleucina-17/antagonistas & inhibidores , Interleucina-17/fisiología , Infecciones por Orthomyxoviridae/inmunología , Neumonía Bacteriana/inmunología , Infecciones Estafilocócicas/inmunología , Linfocitos T Colaboradores-Inductores/inmunología , Animales , Células Cultivadas , Predisposición Genética a la Enfermedad , Humanos , Interferón Tipo I/administración & dosificación , Interferón Tipo I/biosíntesis , Interleucina-17/deficiencia , Interleucina-23/antagonistas & inhibidores , Interleucinas/antagonistas & inhibidores , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Infecciones por Orthomyxoviridae/genética , Infecciones por Orthomyxoviridae/microbiología , Neumonía Bacteriana/genética , Neumonía Bacteriana/virología , Transducción de Señal/genética , Transducción de Señal/inmunología , Infecciones Estafilocócicas/genética , Infecciones Estafilocócicas/virología , Staphylococcus aureus/inmunología , Linfocitos T Colaboradores-Inductores/metabolismo , Linfocitos T Colaboradores-Inductores/virología , Interleucina-22
7.
J Immunol ; 187(10): 5402-7, 2011 Nov 15.
Artículo en Inglés | MEDLINE | ID: mdl-22003199

RESUMEN

IL-23 is required for the IL-17 response to infection with Mycobacterium tuberculosis, but is not required for the early control of bacterial growth. However, mice deficient for the p19 component of IL-23 (Il23a(-/-)) exhibit increased bacterial growth late in infection that is temporally associated with smaller B cell follicles in the lungs. Cxcl13 is required for B cell follicle formation and immunity during tuberculosis. The absence of IL-23 results in decreased expression of Cxcl13 within M. tuberculosis-induced lymphocyte follicles in the lungs, and this deficiency was associated with increased cuffing of T cells around the vessels in the lungs of these mice. Il23a(-/-) mice also poorly expressed IL-17A and IL-22 mRNA. These cytokines were able to induce Cxcl13 in mouse primary lung fibroblasts, suggesting that these cytokines are likely involved in B cell follicle formation. Indeed, IL-17RA-deficient mice generated smaller B cell follicles early in the response, whereas IL-22-deficient mice had smaller B cell follicles at an intermediate time postinfection; however, only Il23a(-/-) mice had a sustained deficiency in B cell follicle formation and reduced immunity. We propose that in the absence of IL-23, expression of long-term immunity to tuberculosis is compromised due to reduced expression of Cxcl13 in B cell follicles and reduced ability of T cells to migrate from the vessels and into the lesion. Further, although IL-17 and IL-22 can both contribute to Cxcl13 production and B cell follicle formation, it is IL-23 that is critical in this regard.


Asunto(s)
Centro Germinal/inmunología , Centro Germinal/patología , Interleucina-23/fisiología , Mycobacterium tuberculosis/inmunología , Tuberculosis Pulmonar/inmunología , Tuberculosis Pulmonar/patología , Animales , Células Cultivadas , Quimiocina CXCL13/biosíntesis , Centro Germinal/microbiología , Interleucina-23/deficiencia , Interleucina-23/genética , Pulmón/inmunología , Pulmón/microbiología , Pulmón/patología , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Mycobacterium tuberculosis/crecimiento & desarrollo , Factores de Tiempo , Tuberculosis Pulmonar/microbiología
8.
Cytokine ; 55(3): 372-9, 2011 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-21669537

RESUMEN

Three cytokines use the IL-12p40 cytokine subunit namely: IL-12p70 (IL-12-comprised of IL-12p40 and IL-12p35), IL-23 (comprised of the IL-12p40 and IL-23p19 subunits) and homodimeric IL-12p40 (IL-12(p40)(2)). Following activation, immature dendritic cells (DCs) upregulate the chemokine receptor Chemokine-C-Receptor 7 (CCR7), and migrate in response to homeostatic chemokines such as chemokine (C-C motif) ligand 19 (CCL19). Induction of the cytokine IL-12p40 in response to pathogen-exposure, likely in its homodimeric form, is one of the primary events that mediates migration of DCs in response to CCL19. Here we show that following exposure to Francisella tularensis Live Vaccine Strain (LVS), DCs produce IL-12p40 and promote the migration of DCs to the chemokine CCL19 in an IL-12Rß1- and IL-12p(40)(2)-dependent manner. Induction of IL-12p40 and resulting chemokine responsiveness in DCs is TLR2-dependent and coincides with the uptake of F. tularensis LVS and activation of DCs. Importantly, we show that IL-12Rß1 signaling is required for DC migration from the lung to the draining lymph node following F. tularensis LVS exposure and coincides with accumulation of IL-12p40 expressing DCs in the draining lymph nodes. Together, these findings illustrate that IL-12p40 is induced rapidly in response to F. tularensis LVS and is required for DC migration through an IL-12Rß1-IL-12(p40)(2) dependent mechanism.


Asunto(s)
Movimiento Celular , Células Dendríticas/inmunología , Francisella tularensis/inmunología , Sudunidad beta 1 del Receptor de Interleucina-12/inmunología , Subunidad p40 de la Interleucina-12/biosíntesis , Subunidad p40 de la Interleucina-12/inmunología , Animales , Quimiocina CCL19/inmunología , Quimiocina CCL19/metabolismo , Células Dendríticas/citología , Pulmón/inmunología , Ganglios Linfáticos/inmunología , Activación de Linfocitos , Ratones , Ratones Endogámicos C57BL , Receptores CCR7/inmunología , Transducción de Señal/inmunología , Receptor Toll-Like 2/inmunología
9.
Cytokine Growth Factor Rev ; 24(2): 105-13, 2013 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-23168132

RESUMEN

Mycobacterium tuberculosis (Mtb) is the intracellular pathogen that causes the disease, tuberculosis. Chemokines and chemokine receptors are key regulators in immune cell recruitment to sites of infection and inflammation. This review highlights our recent advances in understanding the role of chemokines and chemokine receptors in cellular recruitment of immune cells to the lung, role in granuloma formation and host defense against Mtb infection.


Asunto(s)
Quimiocinas/inmunología , Tuberculosis Pulmonar/inmunología , Inmunidad Adaptativa , Animales , Humanos , Inmunidad Innata , Pulmón/inmunología , Mycobacterium tuberculosis
10.
J Clin Invest ; 123(2): 712-26, 2013 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-23281399

RESUMEN

One third of the world's population is infected with Mycobacterium tuberculosis (Mtb). Although most infected people remain asymptomatic, they have a 10% lifetime risk of developing active tuberculosis (TB). Thus, the current challenge is to identify immune parameters that distinguish individuals with latent TB from those with active TB. Using human and experimental models of Mtb infection, we demonstrated that organized ectopic lymphoid structures containing CXCR5+ T cells were present in Mtb-infected lungs. In addition, we found that in experimental Mtb infection models, the presence of CXCR5+ T cells within ectopic lymphoid structures was associated with immune control. Furthermore, in a mouse model of Mtb infection, we showed that activated CD4+CXCR5+ T cells accumulated in Mtb-infected lungs and produced proinflammatory cytokines. Mice deficient in Cxcr5 had increased susceptibility to TB due to defective T cell localization within the lung parenchyma. We demonstrated that CXCR5 expression in T cells mediated correct T cell localization within TB granulomas, promoted efficient macrophage activation, protected against Mtb infection, and facilitated lymphoid follicle formation. These data demonstrate that CD4+CXCR5+ T cells play a protective role in the immune response against TB and highlight their potential use for future TB vaccine design and therapy.


Asunto(s)
Tuberculosis Latente/inmunología , Receptores CXCR5/metabolismo , Linfocitos T Colaboradores-Inductores/inmunología , Tuberculosis Pulmonar/inmunología , Animales , Citocinas/biosíntesis , Modelos Animales de Enfermedad , Femenino , Granuloma del Sistema Respiratorio/inmunología , Humanos , Mediadores de Inflamación/metabolismo , Pulmón/inmunología , Pulmón/microbiología , Activación de Linfocitos , Tejido Linfoide/inmunología , Activación de Macrófagos , Masculino , Ratones , Ratones Noqueados , Ratones Transgénicos , Subgrupos de Linfocitos T/inmunología
11.
Semin Immunopathol ; 32(1): 79-90, 2010 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-20112107

RESUMEN

T helper type 17 (Th17) cells are a distinct lineage of T cells that produce the effector molecules IL-17, IL-17F, IL-21, and IL-22. Although the role of Th17 cells in primary immune responses against infections is well documented, there is growing evidence that the Th17 lineage maybe critical for vaccine-induced memory immune responses against infectious diseases. Here, we summarize recent progress in our understanding of the role of IL-17 in vaccine-induced immunity.


Asunto(s)
Inmunidad Activa/inmunología , Memoria Inmunológica/inmunología , Subgrupos de Linfocitos T/inmunología , Linfocitos T Colaboradores-Inductores/inmunología , Animales , Humanos , Vacunación
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