Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 8 de 8
Filtrar
1.
Org Biomol Chem ; 15(20): 4456-4463, 2017 May 23.
Artículo en Inglés | MEDLINE | ID: mdl-28485737

RESUMEN

(-)-ß-Caryophyllene was decorated with a privileged ß-lactam motif and subsequently converted into highly diverse scaffolds via remodeling of the ring system. The structures were defined by spectroscopic data, X-ray diffraction analysis, and experimental and calculated ECD data. Compound 19 displayed the most potent activity against the rice blast fungus, while 6 had a more potent α-glucosidase inhibition than the drug acarbose. These findings demonstrate a concise protocol to exploit natural product-driven diversity.


Asunto(s)
Antifúngicos/farmacología , Productos Biológicos/farmacología , Inhibidores de Glicósido Hidrolasas/farmacología , Magnaporthe/efectos de los fármacos , Sesquiterpenos/farmacología , beta-Lactamas/farmacología , Antifúngicos/síntesis química , Antifúngicos/química , Productos Biológicos/síntesis química , Productos Biológicos/química , Relación Dosis-Respuesta a Droga , Inhibidores de Glicósido Hidrolasas/síntesis química , Inhibidores de Glicósido Hidrolasas/química , Humanos , Modelos Moleculares , Conformación Molecular , Oryza/microbiología , Sesquiterpenos Policíclicos , Teoría Cuántica , Sesquiterpenos/química , Relación Estructura-Actividad , alfa-Glucosidasas/metabolismo , beta-Lactamas/química
2.
J Adv Res ; 2024 Jan 18.
Artículo en Inglés | MEDLINE | ID: mdl-38244773

RESUMEN

BACKGROUND: Pancreatic cancer, referred to as the "monarch of malignancies," is a neoplastic growth mostly arising from the epithelial cells of the pancreatic duct and acinar cells. This particular neoplasm has a highly unfavorable prognosis due to its marked malignancy, inconspicuous initial manifestation, challenging early detection, rapid advancement, and limited survival duration. Cellular immunotherapy is the ex vivo culture and expansion of immune effector cells, granting them the capacity to selectively target malignant cells using specialized techniques. Subsequently, these modified cells are reintroduced into the patient's organism with the purpose of eradicating tumor cells and providing therapeutic intervention for cancer. PRESENT SITUATION: Presently, the primary cellular therapeutic modalities employed in the treatment of pancreatic cancer encompass CAR T-cell therapy, TCR T-cell therapy, NK-cell therapy, and CAR NK-cell therapy. AIM OF REVIEW: This review provides a concise overview of the mechanisms and primary targets associated with various cell therapies. Additionally, we will explore the prospective outlook of cell therapy in the context of treating pancreatic cancer.

3.
Zhonghua Nan Ke Xue ; 16(3): 250-3, 2010 Mar.
Artículo en Zh | MEDLINE | ID: mdl-20369555

RESUMEN

OBJECTIVE: To observe the clinical effects of using the Chinese Shang Ring in circumcision children with either phimosis or redundant prepuce, and to investigate its superiority over the similar devices available. METHODS: A total of 824 children with phimosis or redundant prepuce underwent circumcision with the Shang Ring. The clinical data were assessed concerning the duration of the procedure, incidence of post-operative complications, time of recovery and appearance of the penis. RESULTS: The procedure duration was (2.6 +/-1.2) min, and the complications included infection in 4 (0.6%), edema in 21 (3.2%), delayed removal of the ring in 10 (1.5%), redundant and asymmetric mucosa attributable to performance in 6 (0.9%) of the cases. The wounds healed and the rings were removed at 13.4 +/- 5.8 days after circumcision, with well-smoothed incision and good cosmetic results. CONCLUSION: Child circumcision with the Chinese Shang Ring is easy and simple in performance, with less operative time, fewer complications and better cosmetic results.


Asunto(s)
Circuncisión Masculina/instrumentación , Fimosis/cirugía , Adolescente , Niño , Preescolar , Circuncisión Masculina/métodos , Humanos , Masculino , Pene/cirugía , Resultado del Tratamiento
4.
Nat Commun ; 10(1): 922, 2019 02 25.
Artículo en Inglés | MEDLINE | ID: mdl-30804501

RESUMEN

Fusarium graminearum is a destructive wheat pathogen. No fully resistant cultivars are available. Knowledge concerning the molecular weapons of F. graminearum to achieve infection remains limited. Here, we report that deletion of the putative secondary metabolite biosynthesis gene cluster fg3_54 compromises the pathogen's ability to infect wheat through cell-to-cell penetration. Ectopic expression of fgm4, a pathway-specific bANK-like regulatory gene, activates the transcription of the fg3_54 cluster in vitro. We identify a linear, C- terminally reduced and D-amino acid residue-rich octapeptide, fusaoctaxin A, as the product of the two nonribosomal peptide synthetases encoded by fg3_54. Chemically-synthesized fusaoctaxin A restores cell-to-cell invasiveness in fg3_54-deleted F. graminearum, and enables colonization of wheat coleoptiles by two Fusarium strains that lack the fg3_54 homolog and are nonpathogenic to wheat. In conclusion, our results identify fusaoctaxin A as a virulence factor required for cell-to-cell invasion of wheat by F. graminearum.


Asunto(s)
Fusarium/metabolismo , Fusarium/patogenicidad , Oligopéptidos/metabolismo , Enfermedades de las Plantas/microbiología , Triticum/microbiología , Cotiledón/microbiología , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Fusarium/genética , Oligopéptidos/genética , Péptido Sintasas/genética , Péptido Sintasas/metabolismo , Virulencia
5.
Nat Prod Res ; 29(4): 345-8, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-25103127

RESUMEN

Six known compounds, isoroquefortine C (1), griseofulvin (2), ergosterol peroxide (3), 3ß-hydroxy-(22E,24R)-ergosta-5,8,22-trien-7-one (4), cerevisterol (5) and (22E,24R)-6ß-methoxyergosta-7,22-diene-3ß,5α-diol (6), were produced by the fungus Penicillium brasilianum, and their structures were elucidated by spectroscopic methods. This is the first report on isoroquefortine C as naturally occurring compound. Their bioactivities against five phytopathogenic fungi (Gibeberalla saubinetti, Fusarium solani, Botrytis cinerea, Colletotrichum gloeosporioides and Alternaria solani) and four pathogenic bacteria (Escherichia coli, Bacillus subtilis, Staphyloccocus aureus and Bacillus cereus), as well as allelopathic activities on Raphanus sativus were tested. Compound 1 exhibited a remarkable antifungal activity with minimum inhibitory concentration (MIC) of 12.5 µM against C. gloeosporioides, in comparison with positive control hymexazol (MIC 25 µM). Compound 2 displayed strong inhibitory effects on the growth of A. solani and S. aureus with MIC of 3.13 µM for each. Compounds 2 and 3 displayed a significant growth-inhibition activity on R. sativus.


Asunto(s)
Alelopatía , Antibacterianos/farmacología , Antifúngicos/farmacología , Penicillium/química , Antibacterianos/aislamiento & purificación , Antifúngicos/aislamiento & purificación , Bacterias/efectos de los fármacos , Hongos/efectos de los fármacos , Griseofulvina/aislamiento & purificación , Griseofulvina/farmacología , Compuestos Heterocíclicos de 4 o más Anillos/aislamiento & purificación , Compuestos Heterocíclicos de 4 o más Anillos/farmacología , Indoles/aislamiento & purificación , Indoles/farmacología , Estructura Molecular , Piperazinas/aislamiento & purificación , Piperazinas/farmacología , Raphanus/efectos de los fármacos
6.
Curr Med Chem ; 22(19): 2375-91, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-25994862

RESUMEN

Cyathane diterpenoids, occurring exclusively in higher basidiomycete (mushrooms), represent a structurally diverse class of natural products based on a characteristic 5-6-7 tricyclic carbon scaffold, including 105 members reported to date. These compounds show a diverse range of biological activities, such as antimicrobial, anti-MRSA, agonistic toward the kappa-opioid receptor, antiinflammatory, anti-proliferative and nerve growth factor (NGF)-like properties. The present review focuses on the structure diversity, structure elucidation and biological studies of these compounds, including mechanisms of actions and structure-activity relationships (SARs). In addition, new progress in chemical synthesis of cyathane diterpenoids is discussed.


Asunto(s)
Basidiomycota/química , Productos Biológicos/farmacología , Diterpenos/farmacología , Antiinflamatorios/farmacología , Antineoplásicos/farmacología , Productos Biológicos/síntesis química , Productos Biológicos/química , Productos Biológicos/aislamiento & purificación , Diterpenos/síntesis química , Diterpenos/química , Diterpenos/aislamiento & purificación , Estructura Molecular , Factor de Crecimiento Nervioso/antagonistas & inhibidores , Factor de Crecimiento Nervioso/biosíntesis , Relación Estructura-Actividad
7.
Nat Prod Res ; 27(4-5): 496-9, 2013 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-22765159

RESUMEN

Eleven compounds were isolated from the culture of an endophytic bacterium Pseudomonas brassicacearum subsp. Neoaurantiaca in Salvia miltiorrhiza Bunge. Their structures were elucidated by spectroscopic methods as cyclo-(Gly-L-Ala) (1), cyclo-(L-Ala-L-Ala) (2), cyclo-(L-Pro-Gly) (3), cyclo-(L-Pro-L-Ser) (4), cyclo-(L-Ala-trans-4-hydroxy-L-Pro) (5), cyclo-(L-Val-L-Pro) (6), cyclo-(Gly-L-Tyr) (7), cyclo-(L-Ala-L-Tyr) (8), cyclo-(L-Tyr-trans-4-hydroxy-L-Pro) (9), 3-methylhydantoin (10) and 2-piperidinone (11). All these compounds were isolated from this bacterium for the first time. The brine shrimp lethality, antifungal and antibacterial activities of these compounds were evaluated. The results indicated that some cyclodipeptides may play an important role in plant-bacteria interactions.


Asunto(s)
Alcaloides/química , Pseudomonas/química , Pseudomonas/fisiología , Salvia miltiorrhiza/microbiología , Animales , Antibacterianos/química , Antibacterianos/farmacología , Antifúngicos/química , Antifúngicos/farmacología , Artemia/efectos de los fármacos , Estructura Molecular
8.
J Agric Food Chem ; 61(47): 11447-52, 2013 Nov 27.
Artículo en Inglés | MEDLINE | ID: mdl-24188331

RESUMEN

On the basis of the OSMAC (one strain-many compounds) strategy, 14 indole diketopiperazine (DKP) alkaloids, including spirotryprostatins (1-3), tryprostatins (4-6), and cyclotryprostatins (7-14), were isolated from the endophyte Aspergillus fumigatus associated with Melia azedarach L. Their structures were identified by nuclear magnetic resonance and electrospray ionization mass spectrometry data. All the indole DKPs were evaluated for plant growth regulation using the lettuce (Lactuca sativa) seedling growth bioassay, which showed the plant growth influence of the seedling. Among these compounds tested, a tryprostatin-type compound, brevianamide F (6), was identified as a new type of natural potential plant growth inhibitor with a response index (RI) higher than that of the positive control glyphosate, a broad-spectrum systemic herbicide. 6 can also inhibit turnip (Raphanus sativus) shoot and root elongation with RIs of -0.76 and -0.70, respectively, at 120 ppm, and it strongly inhibits amaranth (Amaranthus mangostanus) seedling growth with a high RI of -0.9 at 40 ppm. The structure-allelopathic activity relationship analysis of these isolated alkaloids indicates that tryprostatin-type alkaloids without the C5 prenyl and methoxy group give the most potent inhibition of seedling growth. Brevianamide F (6) could be used to develop a natural eco-friendly herbicide.


Asunto(s)
Aspergillus fumigatus/química , Dicetopiperazinas/farmacología , Herbicidas/farmacología , Amaranthus/efectos de los fármacos , Cromatografía Líquida de Alta Presión , Dicetopiperazinas/química , Endófitos/química , Germinación/efectos de los fármacos , Herbicidas/química , Alcaloides Indólicos/química , Alcaloides Indólicos/farmacología , Indoles/química , Indoles/farmacología , Lactuca/efectos de los fármacos , Lactuca/crecimiento & desarrollo , Espectroscopía de Resonancia Magnética , Melia/microbiología , Estructura Molecular , Reguladores del Crecimiento de las Plantas/farmacología , Raphanus/efectos de los fármacos , Plantones/efectos de los fármacos , Espectrometría de Masa por Ionización de Electrospray , Relación Estructura-Actividad
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA