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Anal Bioanal Chem ; 406(22): 5507-12, 2014 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-24928115

RESUMEN

In human medicine, procalcitonin (PCT) is a very common and well-established biomarker for sepsis. Even though sepsis is also a leading cause of death in foals and adult horses, up to now, no data about the role of equine PCT in septic horses has been available. Based on monoclonal antibodies targeted against human PCT, we report here the development of a sandwich ELISA for the quantification of equine PCT in equine plasma samples. The ELISA was characterized for intra- and interassay variance and a working range from 25 to 1,000 ng mL(-1) was defined as within this range; both intra- and interassay variances were below 15 %. The target recovery ranged between 73 and 106 %. The ELISA was used to determine the equine PCT concentration in 24 healthy and 5 septic horses to show the potential for clinical evaluation of equine PCT. Significantly different (P = 0.0006) mean equine PCT concentrations were found for the healthy control group and the sepsis group (47 and 8,450 ng mL(-1)).


Asunto(s)
Biomarcadores/sangre , Calcitonina/sangre , Ensayo de Inmunoadsorción Enzimática/métodos , Precursores de Proteínas/sangre , Sepsis/diagnóstico , Animales , Anticuerpos Monoclonales/química , Péptido Relacionado con Gen de Calcitonina , Femenino , Caballos , Humanos , Inflamación , Masculino , Curva ROC , Proteínas Recombinantes/química , Reproducibilidad de los Resultados , Sepsis/sangre
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