RESUMEN
The expression levels of heat shock protein 70 (HSP70) from peripheral lymphocytes of the patients with allergic rhinitis (AR) and the clinical implication were investigated. In the morning, 3 ml of fasting venous blood was taken out. The lymphocytes were isolated by using Ficoll-Hypaque and the expression of HSP70 in the lymphocytes was detected by using Western blot. In the AR patients the HSP70 level (41.49 +/- 15.77 integrated optical density, IOD) were significantly higher than that in the control group (23.89 +/- 10.13 IOD, P < 0.05). Western blot demonstrated that HSP70 bands in AR patients were more intensive than those in the control group. It was concluded that the elevated HSP70 level in peripheral lymphocytes of the AR patients might contribute to the development of AR.
Asunto(s)
Proteínas HSP70 de Choque Térmico/biosíntesis , Linfocitos/metabolismo , Rinitis Alérgica Estacional/sangre , Adulto , Femenino , Regulación de la Expresión Génica , Proteínas HSP70 de Choque Térmico/sangre , Proteínas HSP70 de Choque Térmico/genética , Humanos , MasculinoRESUMEN
OBJECTIVE: To investigate the immunoregulation of dendritic cells in allergic rhinitis. METHOD: Adopting the cell culture and the enzyme linked immunosorbant assay to examine the patients' Th1/Th2 in the blood. Observing that how is the DC's function in immunoregulation of allergic rhinitis. RESULT: The concentration of the IFN-gamma advanced obviously in post-stimulatory supernatant liquid after using the mature DC. CONCLUSION: The mature dendritic cells can polarize naive T-cells into Th1 effector cells, and are recognized as having a central role in the establishment of T-cell memory and peripheral immune tolerance. DC could be exploited as targets for therapy in asthma and allergic rhinitis.