RESUMEN
Osthole (also known as Osthol) is the main anti-inflammatory coumarin found in Cnidium monnieri and severs as the exclusive quality-controlled component according the Chinese Pharmacopoeia. However, its underlying anti-inflammatory mechanism remains unknown. In this study, we demonstrated that Osthole treatment significantly inhibited the generation of TNF-α, but not IL-6 in the classical LPS-stimulated RAW264.7 macrophage model. In addition, LPS induced the activation of both MAPK and NF-κB signalling pathways, of which the former was dose-dependently restrained by Osthole via suppressing the phosphorylation of JNK and P38 proteins, while the phosphorylation of IκB and P65 proteins remained unaffected. Interestingly, Osthole dose-dependently up-regulated the expression of the key cholinergic anti-inflammatory pathway regulator α7nAChR, and the TNF-α inhibition effect of Osthole was also significantly alleviated by the treatment of α7nAChR antagonist methylbetaine. These results demonstrate that Osthole may regulate TNF-α by promoting the expression of α7nAChR, thereby activate the vagus nerve-dependent cholinergic anti-inflammatory pathway.
Asunto(s)
Factor de Necrosis Tumoral alfa , Receptor Nicotínico de Acetilcolina alfa 7 , Humanos , Regulación hacia Arriba , Lipopolisacáridos/farmacología , Neuroinmunomodulación , Cumarinas/farmacología , Antiinflamatorios/farmacología , Inflamación/tratamiento farmacológicoRESUMEN
The present study analyzed the chemical constituents in dried roots of Dolomiaea souliei. Chromatographic methods, such as normal-phase, and reversed-phase column chromatography, TLC, and preparative HPLC, were applied to separate and purify the petroleum ether extract of D. souliei. The structures of the purified constituents were identified by multiple spectroscopic methods including 1 D NMR, 2 D NMR, IR, UV, and HR-ESI-MS. Fourteen triterpenoids were obtained and identified as bauer-8-ene-3,11-dione-7α-ol(1), bauer-8-ene-3-one-7α,11α-diol(2), 3-oxo-11α-hydroxy-urs-12-ene(3), 3-oxour-12-ene-1ß,11α-diol(4), 3ß,11α-dihydroxy-urs-12-ene(5), taraxast-20-ene-3ß,30-diol(6), 28-hydroxy-3-oxo-12-ursene(7), 3ß-hydroxytaraxast-20-ene-30-aldehyde(8), urs-12-ene-2α,3ß,28-triol(9), 20-hydroxylupan-3-one(10), monogynol A(11), obtusalin(12), 3-oxo, 11α-hydroxy-olean-12-ene(13), and isocabralealactone(14). Among them, compounds 1 and 2 were new compounds. Compounds 4-10, 12, and 14 were isolated from this genus for the first time. Compounds 3 and 11 were obtained from D. souliei for the first time.
Asunto(s)
Asteraceae , Triterpenos , Triterpenos/química , Espectroscopía de Resonancia Magnética , Solventes , Estructura MolecularRESUMEN
Two new abietane diterpenoids (1-2) and 13 known compounds (3-15) were characterized from the branches and leaves of Abies faxoniana. The chemical structures of the new diterpenoids (1-2) were determined through the analysis of various 1D/2D NMR techniques. Compound 3 is 151,617-trinor-abietane diterpenoid conjugated with a three-membered epoxide ring. The isolated diterpenoids were tested for their cytotoxicities. Among them, compound 3 exhibited the strongest antiproliferative effect against human hepatoma cell SMMC7721 with an IC50 value of 12.5 µM. To elucidate the preliminary mechanism responsible for compound 3-induced inhibition of cell proliferation, we investigated the effects of compound 3 on apoptosis, cell cycle progression, and reactive oxygen species generation in SMMC7721 cells. The results showed that compound 3 induced cell apoptosis and excessive ROS production, but did not change the cell cycle distribution in SMMC7721 cells.
Asunto(s)
Abies/química , Abietanos/aislamiento & purificación , Abietanos/farmacología , Antineoplásicos Fitogénicos/aislamiento & purificación , Antineoplásicos Fitogénicos/farmacología , Apoptosis/efectos de los fármacos , Carcinoma Hepatocelular/tratamiento farmacológico , Neoplasias Hepáticas/tratamiento farmacológico , Abietanos/química , Antineoplásicos Fitogénicos/química , Ciclo Celular/efectos de los fármacos , Humanos , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Hojas de la Planta/químicaRESUMEN
Two new long-chain unsaturated compounds, (2E, 6E)-10-methoxy-3,7-dimethyl-10-oxodeca-2,6-dienoic acid (1) and (2E, 6E)-3,7,11-trimethyldodeca-2,6-dienedioic acid (2), together with seven known compounds were isolated from a marine-derived Streptomyces sp. Their structures were determined by spectroscopic methods, including 2D NMR techniques. Compounds 1 and 2 were investigated for their antibacterial activities.
Asunto(s)
Antibacterianos/aislamiento & purificación , Ácidos Grasos Insaturados/aislamiento & purificación , Streptomyces/química , Antibacterianos/química , Antibacterianos/farmacología , Ácidos Grasos Insaturados/química , Ácidos Grasos Insaturados/farmacología , Biología Marina , Pruebas de Sensibilidad Microbiana , Estructura Molecular , Resonancia Magnética Nuclear BiomolecularRESUMEN
Phytochemical investigation on the stems of Kadsura renchangiana led to the isolation of two new sesquiterpenoids, renchangianins F and G (1 and 2). Their structures were elucidated by spectroscopic methods, including 2D NMR techniques. The in vitro cytotoxic activities of the isolates were studied against HepG2, A549, and LN229 cancer cell lines.
Asunto(s)
Antineoplásicos Fitogénicos/aislamiento & purificación , Kadsura/química , Sesquiterpenos/aislamiento & purificación , Antineoplásicos Fitogénicos/química , Antineoplásicos Fitogénicos/farmacología , Ensayos de Selección de Medicamentos Antitumorales , Células Hep G2 , Humanos , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Tallos de la Planta/química , Sesquiterpenos/química , Sesquiterpenos/farmacologíaRESUMEN
A new napthalenone, rumexone A (1), was isolated from the roots of Rumex nepalensis. The structure of 1 was elucidated by extensive spectroscopic analyses, including 1D and 2D NMR spectra and MS data. Its cytotoxic effect was evaluated using four clinically relevant human cancer cell lines, gastric carcinoma SGC7901, breast carcinoma MDA-MB-231, lung carcinoma A549, and hepatocellular carcinoma HepG2.
Asunto(s)
Naftalenos/aislamiento & purificación , Raíces de Plantas/química , Rumex/química , Línea Celular Tumoral , Humanos , Espectroscopía de Resonancia Magnética , Estructura Molecular , Naftalenos/farmacología , Fitoquímicos/aislamiento & purificación , Fitoquímicos/farmacologíaRESUMEN
A phytochemical investigation on the roots of Campylotropis hirtella afforded nine new isoflavones (3-9, 12, 15), two new isoflavans (10 and 11), one new coumestan (1), and three new prenylated benzoic acid derivatives (2, 13, 14), together with twenty-four known compounds. Their structures were established by spectroscopic analysis and circular dichroism data. The isolated compounds were also evaluated for their antibacterial activities against Enterococcus faecalis, Salmonella gallinarum, Streptococcus suis, Streptococcus agalactiae, Aeromonas hydrophila, Pseudomonas aeruginosa, Bacillus subtilis, Riemerella anatipestifer, and Vibrio alginolyticus.
Asunto(s)
Antibacterianos/aislamiento & purificación , Fabaceae/química , Extractos Vegetales/aislamiento & purificación , Antibacterianos/química , Antibacterianos/farmacología , Pruebas de Sensibilidad Microbiana , Estructura Molecular , Extractos Vegetales/química , Extractos Vegetales/farmacología , Raíces de Plantas/químicaRESUMEN
Eighteen terpenoids (1-18) were isolated from Illicium merrillianum. Compound 1 was identified as new compound, and its structure was established by comprehensive spectroscopic analysis and single-crystal X-ray diffraction. All compounds were evaluated for nerve growth factor (NGF)-mediated neurite outgrowth activity using rat pheochromocytoma (PC12) cells as a model system of neuronal differentiation. Compounds 1, 3, 18 showed significant neurite outgrowth-promoting activity in the presence of 20 ng/ml NGF in a dose-dependent manner at concentrations of 1-100 µM after 24-h treatment. Subtle difference of functional groups at C-2 position in hopane-type triterpene resulted in enormous bioactivity difference, compound 1 was neurotrophic but 2 was cytotoxic.
Asunto(s)
Illicium/química , Terpenos/aislamiento & purificación , Terpenos/farmacología , Animales , Cristalografía por Rayos X , Conformación Molecular , Estructura Molecular , Factor de Crecimiento Nervioso , Neuritas/efectos de los fármacos , Proyección Neuronal/efectos de los fármacos , Neuronas/metabolismo , Células PC12 , Hojas de la Planta/química , Ratas , Terpenos/química , Difracción de Rayos XRESUMEN
Two new compounds including one new sesquiterpenoid and one new monoterpenoid, together with 10 known compounds were isolated from the whole plants of Ainsliaea latifolia. The structures of these compounds were elucidated by analysis of spectroscopic data. All compounds were evaluated for their cytotoxic activities.
Asunto(s)
Antineoplásicos Fitogénicos/aislamiento & purificación , Antineoplásicos Fitogénicos/farmacología , Asteraceae/química , Medicamentos Herbarios Chinos/aislamiento & purificación , Medicamentos Herbarios Chinos/farmacología , Terpenos/aislamiento & purificación , Terpenos/farmacología , Antineoplásicos Fitogénicos/química , Ensayos de Selección de Medicamentos Antitumorales , Medicamentos Herbarios Chinos/química , Humanos , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Terpenos/químicaRESUMEN
The chemical constituents of Herpetospermum caudigerum were investigated using chromatographic methods, including silica gel column chromatography, Sephadex LH-20 and semi-preparative HPLC. Four compounds were isolated and their structures were elucidated by spectral data and physicochemical properties, which were identified as 2,11-dimethoxy-3,9-dihydroxy-7 H-dibenzo[c,e]oxepin-5-one (1),7,8'-didehydroherpetotriol(2), herpetotrio l(3) and kaempferitrin (4). Among those, compound 1 is one new 7H-dibenzo[c,e]oxepin-5-one, named as herpetolide C.
Asunto(s)
Cucurbitaceae/química , Oxepinas/aislamiento & purificación , Cromatografía Líquida de Alta PresiónRESUMEN
Five pairs of new epimeric lanostane-type triterpenoids, abiespirones A-D (1-4) and G (7), two pairs of new epimeric cycloartane-type triterpenoids, abiespirones E and F (5, 6), and a pair of new epimeric 7(8â9)abeo-spirolanostane abiespirones H (8) with spiro-B/C and -E/F ring systems were isolated from Abies faxoniana as inseparable mixtures of C-23 epimers in a specific proportion. The HPLC plots showed that each purified isomer rapidly equilibrated with the C-23 epimer in solution. The structures of compounds 1-8 were elucidated by analysis of the NMR spectra and single-crystal X-ray diffraction. Compound 6 showed cytotoxicity against three hepatoma cell lines, namely, HepG2, Huh7, and SMMC7721, with IC50 values of 9.8, 7.5, and 10.7 µM, respectively, but exerted low cytotoxicity on normal QSG7701 hepatic cells, indicating its selective cytotoxicity for hepatoma cells. Compound 6 arrests the cell cycle at G2/M and induces cell apoptosis in Huh7 cells. In addition, the generation of reactive oxygen species (ROS) was detected in Huh7 cells when treated with compound 6, and a ROS scavenger partly blocked the effects of compound 6-induced Huh7 cell death, suggesting that compound 6-induced apoptosis is associated with elevated levels of ROS in Huh7 cells.
Asunto(s)
Abies/química , Antineoplásicos Fitogénicos/aislamiento & purificación , Medicamentos Herbarios Chinos/aislamiento & purificación , Compuestos de Espiro/aislamiento & purificación , Triterpenos/aislamiento & purificación , Antineoplásicos Fitogénicos/química , Antineoplásicos Fitogénicos/farmacología , Apoptosis/efectos de los fármacos , Carcinoma Hepatocelular/tratamiento farmacológico , Ciclo Celular/efectos de los fármacos , Ensayos de Selección de Medicamentos Antitumorales , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/farmacología , Humanos , Neoplasias Hepáticas/tratamiento farmacológico , Conformación Molecular , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Especies Reactivas de Oxígeno/metabolismo , Compuestos de Espiro/química , Compuestos de Espiro/farmacología , Estereoisomerismo , Triterpenos/química , Triterpenos/farmacología , Difracción de Rayos XRESUMEN
We report a new type of single InAs quantum dot (QD) embedded at the junction of gold-free branched GaAs/AlGaAs nanowire (NW) grown on silicon substrate. The photoluminescence intensity of such QD is ~20 times stronger than that from randomly distributed QD grown on the facet of straight NW. Sharp excitonic emission is observed at 4.2 K with a line width of 101 µeV and a vanishing two-photon emission probability of g(2)(0) = 0.031(2). This new nanostructure may open new ways for designing novel quantum optoelectronic devices.
Asunto(s)
Nanoestructuras/química , Nanotecnología , Nanocables/química , Puntos Cuánticos , Arsenicales/química , Diseño de Equipo , Galio/química , Indio/química , SilicioRESUMEN
Little is known about the difference of anthropometry and physical fitness between the finalist and eliminated player in the NBA all star slam dunk contest. This study aimed to compare the difference on anthropometric and physical fitness indicator in the combine draft between finalist and eliminated player in the national basketball association all star slam dunk contest. Draft data of 32 basketball players (N = 32, age in draft year: 20.69±2.28 years old, height without shoes: 196.75±8.68 cm, weight: 96.85±10 kg, body fat percentage: 6.07±1.23%) participating in the 2000-2015 draft and 2003-2023 slam dunk contest was selected from national basketball association database. It was classified into finals group (FG) (N = 16) and elimination group (EG) (N = 16). Independent sample t-test with cohen's d was adopted for evaluating the statistical significance of intergroup difference and its effect size. The result indicates that Finalist group was significant less than elimination group on height without shoes (FG vs EG: 193.43±9.47 cm vs 200.06±6.52 cm, P<0.05), standing reach (FG vs EG: 257.66±12.32 cm vs 268.29±10.03 cm, P<0.05) and weight (FG vs EG: 93.38±7.37 kg vs 100.33±11.25 kg, P<0.05). Conversely, compared to elimination group,finalist group has significant better performance on three quarter court sprint (FG vs EG: 3.15±0.1 s vs 3.26±0.12 s, P<0.05), standing vertical jump (FG vs EG: 84.88±5.13 cm vs 78.83±4.9 cm, P<0.05) and max vertical jump (FG vs EG: 102.39±6.47 cm vs 94.79±8.34 cm, P<0.05). However, effect size analysis indicated that height without shoes,standing reach,weight (cohen's d = 0.73-0.959, 0.7≤cohen's d<1.3, moderate) from the anthropometric indicator and three quarter court sprint, standing vertical jump, and max vertical jump (cohen's d = 0.97-1.21, 0.7≤cohen's d<1.3, moderate) from physical fitness indicator has moderate effect size, whereas effect size of body fat percentage, wingspan and lane agility time (cohen's d = 0.31-0.67, 0.3≤cohen's d<0.7, small) was small. To conclude, specific anthropometric and physical fitness indicator shows clear difference between finals group and elimination group. Height without shoes, standing reach,weight in anthropometry and three quarter court sprint, standing vertical jump, and max vertical jump in physical fitness are key indicator to slam dunk performance. In line with the result in the study, NBA staff can select suitable rookies for slam dunk contest. Similiarly, coach from NBA or other basketball league, who want to improve the player's slam dunk performance, should use specific training programs to develop the slam dunk-related indicator.
Asunto(s)
Rendimiento Atlético , Baloncesto , Humanos , Antropometría , Bases de Datos Factuales , Aptitud Física , ZapatosRESUMEN
The purpose of this study was to investigate the absorption kinetics of aconitine, mesaconitine and hypaconitine in rats after oral administration of Sini Tang powder. With cannulate portal and jugular veins cannulated (double-cannulate), conscious moving rats were orally administered Sini Tang. Then samples of portal and systemic blood were collected at the designated periods of time and analyzed for aconitine, mesaconitine and hypaconitine by HPLC. Apparent absorption coefficient of aconitine, mesaconitine and hypaconitine was caculated respectively. The results indicated that the apparent absorption coefficient of aconitine, mesaconitine and hypaconitine come from Sini Tang were 0. 336, 0. 090, 0. 176, respectively, which had some differences among them. It was also suggested that double-cannulated rat was useful for estimating the absorption kinetics of aconitine, mesaconitine and hypaconitine after orally administered Sini Tang by determining the AUC values for drugs in portal and systemic blood samples. The three alkaloids could all be detected in blood, but the absorption differences were existed among the three alkaloids.
Asunto(s)
Alcaloides/farmacocinética , Diterpenos/farmacocinética , Medicamentos Herbarios Chinos/farmacocinética , Absorción , Administración Oral , Alcaloides/administración & dosificación , Alcaloides/sangre , Animales , Área Bajo la Curva , Diterpenos/sangre , Medicamentos Herbarios Chinos/administración & dosificación , Femenino , Cinética , Masculino , Polvos/farmacocinética , Ratas , Ratas Sprague-DawleyRESUMEN
BACKGROUND: A series of long non-coding RNAs (lncRNAs) have been reported to play a crucial role in cancer biology. Some previous studies report that lncRNA CDKN2B-AS1 is involved in some human malignancies. However, its role in hepatocellular carcinoma (HCC) has not been fully deciphered. AIM: To decipher the role of CDKN2B-AS1 in the progression of HCC. METHODS: CDKN2B-AS1 expression in HCC was detected by quantitative real-time polymerase chain reaction. The malignant phenotypes of Li-7 and SNU-182 cells were detected by the CCK-8 method, EdU method, and flow cytometry, respectively. RNA immunoprecipitation was executed to confirm the interaction between CDKN2B-AS1 and E2F transcription factor 1 (E2F1). Luciferase reporter assay and chromatin immunoprecipitation were performed to verify the binding of E2F1 to the promoter of G protein subunit alpha Z (GNAZ). E2F1 and GNAZ were detected by western blot in HCC cells. RESULTS: In HCC tissues, CDKN2B-AS1 was upregulated. Depletion of CDKN2B-AS1 inhibited the proliferation of HCC cells, and the depletion of CDKN2B-AS1 also induced cell cycle arrest and apoptosis. CDKN2B-AS1 could interact with E2F1. Depletion of CDKN2B-AS1 inhibited the binding of E2F1 to the GNAZ promoter region. Overexpression of E2F1 reversed the biological effects of depletion of CDKN2B-AS1 on the malignant behaviors of HCC cells. CONCLUSION: CDKN2B-AS1 recruits E2F1 to facilitate GNAZ transcription to promote HCC progression.
RESUMEN
A new benzofuran-type neolignan (1), two new phenylpropanoids (2 - 3), and one new C21 steroid (4) were isolated from the ethyl acetate extract of the roots of Dolomiaea souliei by chromatographic methods, including silica gel, ODS column chromatography, MPLC, and semi-preparative HPLC. Their structures were identified as dolosougenin A (1), (S)-3-isopropylpentyl (E)-3-(4-hydroxy-3-methoxyphenyl) acrylate (2), (S)-3-isopropylpentyl (Z)-3-(4-hydroxy-3-methoxyphenyl) acrylate (3) and dolosoucin A (4) through various spectroscopic techniques including 1D NMR, 2D NMR, IR, UV, HR ESI MS, ORD, and computational ORD methods.
RESUMEN
Plants belonging to the genus Broussonetia (Moraceae), which grow naturally in Asian and Pacific countries, have long been used in traditional Chinese medicine to treat various conditions, particularly tinea, dysentery, hernia and oedema. Various parts of plants of this genus such as the fruits, bark, leaves, flowers and roots are used medicinally. According to Flora of China, the inner bark fibre is used in making paper, and the wood is used for furniture. Several active compounds including coumarins, polyphenols, alkaloids, flavonoids, glycosides, lignans and diterpenes have been isolated from the plants of this genus in the past few years, and some members of these constituents have been demonstrated to exert antioxidant, antiinflammatory, antiplatelet, antityrosinase, antimicrobial and antinociceptive effects. Antidiabetic and antitumoural properties seem possible, but need further support. Due to the lack of clinical trials, there are few published reports on target-organ toxicity or side effects. Currently, some East Asian nations such as China, South Korea and Japan have shown increased interest in these plants. This article reviews the phytochemical and potential beneficial therapeutic effects of plants of this genus reported since 2000.
Asunto(s)
Broussonetia/química , Fitoterapia , Extractos Vegetales/farmacología , Animales , Humanos , Extractos Vegetales/uso terapéuticoRESUMEN
The effect of occlusion on the temporomandibular joint (TMJ) is debated. By inserting rubber-bands that were replaced by self-curing resin one week later, the left maxillary and the right mandibular first-molars were moved and kept mesially in Sprague-Dawley (SD) rats in both experimental I (EXP-I) and II (EXP-II) groups, aiming to establish a non-matching cusp-to-fossa occlusal relation. Four weeks later, the left maxillary and the right mandibular third-molars were moved and kept distally in the EXP-II group. Degenerative changes, typically as a cell-free area, were observed in TMJs of the EXP groups. Binary logistical analysis indicated that the odds ratio of EXP group, EXP-II vs. EXP-I, on the incidence of a cell-free area, was 2.8 (p=.036). Time point, gender, and side did not have such effects (p>0.05). The results indicate that the persistence of more scattered non-matching cusp-to-fossa occlusion is more harmful to the condylar cartilage in terms of the incidence of degenerative changes.
Asunto(s)
Cartílago Articular/patología , Maloclusión/complicaciones , Cóndilo Mandibular/patología , Osteoartritis/etiología , Trastornos de la Articulación Temporomandibular/etiología , Animales , Muerte Celular , Condrocitos/patología , Diastema/patología , Femenino , Hipertrofia , Masculino , Diente Molar/patología , Tercer Molar/patología , Osteoartritis/patología , Ratas , Ratas Sprague-Dawley , Trastornos de la Articulación Temporomandibular/patología , Técnicas de Movimiento Dental/efectos adversosRESUMEN
Three undescribed prenylated benzoic acid derivatives berberisides A-C (1-3) and a new clerodane glycoside berberiside D (4) were isolated from Berberis tsarica Aherndt. Their structures were elucidated on the basis of extensive NMR and HR-ESI-MS analysis. The in vitro cytotoxic activities of all isolates were studied against lung carcinoma A549, hepatocellular carcinoma HepG2 and breast carcinoma MDA-MB-231 cell lines. Among them, compounds 1 and 4 exhibited anti-proliferative effects against three tumor cell lines with IC50 ranging from 28.97 ± 2.18 to 35.83 ± 0.72 µM.
Asunto(s)
Berberis , Diterpenos de Tipo Clerodano , Ácido Benzoico , Línea Celular Tumoral , Diterpenos de Tipo Clerodano/farmacología , Glicósidos/farmacología , Humanos , Estructura MolecularRESUMEN
OBJECTIVE: To analyze the expression of toll-like receptor 9 mRNA, TNF (tumor necrosis factor)-α and IL (interleukin)-6 after the stimulation of murine microglia (BV2) by hepatitis C virus (HCV) so as to elucidate the immune pathogenesis of HCV in the injury of central nervous system (CNS). METHODS: BV2 cells were cultured in vitro and divided into 3 groups: HCV-positive serum group, normal serum group (negative control) and blank control group. Each group had 20 samples. The cells and supernatant were collected at 4, 12, 20, 36 h. And TLR9 mRNA was detected by RT-PCR (reverse transcription-polymerase chain reaction) and the levels of TNF-α and IL-6 were detected by ELISA (enzyme-linked immunosorbent assay). Comparison analyses were performed by SPSS13.0 statistical package. RESULTS: (1) RT-PCR showed that there was the expression of TLR9 mRNA in BV2 cells. And the level of TLR9 mRNA in HCV-positive serum group (0.85 ± 0.11, 0.55 ± 0.08, 0.58 ± 0.09, 0.61 ± 0.07) was higher than that in the other groups. The difference had statistical significance (F = 17.258, P < 0.05). It was the highest at 4 h (0.85 ± 0.11). And the difference had statistical significance (F = 7.427, P < 0.05); but there were no statistical significance between normal serum group and control group (P > 0.05); no significant difference existed among 12, 20 and 36 h (P > 0.05). (2) The levels of TNF-α [(926 ± 133), (327 ± 59), (367 ± 69), (354 ± 71) pg/ml] and IL-6 [(125 ± 34), (84 ± 20), (82 ± 24), (87 ± 18) pg/ml] in the HCV-positive serum group were higher than those in the other groups (F = 18.263 - 27.469, P < 0.05); in HCV-positive serum group, the levels of TLR9 mRNA and TNF-α (r = 0.425, P < 0.01)/IL-6 (r = 0.489, P < 0.01) were positively correlated. CONCLUSION: The expression of TLR9 mRNA is elevated after the stimulation of BV2 cells by HCV-positive serum. It peak comes at 4 h. And it may induce the secretions of TNF-α and IL-6. Thus TLR9 may increase the secretions of Th1 and Th2 cytokines so as to participate in the early inherent immune response during the infection of CNS by HCV.