Responses of root-associated fungal community structure of mycorrhizal plants to nitrogen and/or phosphorus addition in a subtropical forest.

Root-associated fungi play a vital role in maintaining nutrient absorption and health of host plants. To compare the responses of root-associated fungal community structures to nitrogen (N) and/or phosphorus (P) additions across differential mycorrhizal types, we collected roots of nine plant species belonging to three mycorrhizal types (arbuscular mycorrhiza, ectomycorrhiza, and ericoid mycorrhiza) under control and N and/or P addition treatments from a subtropical forest, and detected the diversity and community composition of fungi inhabiting roots through the high-throughput sequencing technique. The results showed that root-associated fungal communities of all nine plant species were mainly composed of Basidiomycota and Ascomycota. The relative abundance of Ascomycota and Basidiomycota was significantly lower and higher under the P addition than that under control, respectively. The relative abundance of Ascomycota of ericoid mycorrhizal trees was significantly higher than those of arbuscular mycorrhizal and ectomycorrhizal trees, while the relative abundance of Basidiomycota was significantly lower than the other two mycorrhizal types. Compared with the control, P addition significantly reduced the α-diversity and changed community composition of root-associated fungi across different mycorrhizal plant types, while no effect of N addition or mycorrhizal type was observed. Compared with the control and N addition treatments, NP addition caused root-associated fungal communities of all plants becoming integrally divergent. In addition, the fungal communities of ectomycorrhizal mycorrhizal trees became apparently convergent in comparison with those of arbuscular and ericoid mycorrhizal trees under the NP addition. Collectively, our results highlighted that P was a critical factor influencing community structures of tree root-associated fungi in subtropical forest soils. This study would enhance our understanding of the responses and maintenance mechanisms of plant root-associated fungal diversity under global environmental changes in the subtropical region.

Bacterial communities in the phyllosphere are distinct from those in root and soil, and sensitive to plant species changes in subtropical tree plantations.

Deciphering the local diversity and community composition of plant-associated microorganisms is crucial to predict their ecological functions in forest ecosystems. The differences in microbial diversity and community composition between the aboveground and belowground tree compartments remain largely unknown. Here, we examined bacterial communities in the leaf surface (phyllosphere) and root-associated (root and rhizospheric soil) habitats of 13 tree species. Bacterial richness substantially differed across the three compartments, with the highest value observed in rhizospheric soil. Tree species exerted a significant effect on α-diversity of leaf- and soil- but not root-inhabiting bacteria. Bacterial communities were distinct across habitats and were significantly more divergent in leaf- than in root-associated habitats. Leaf nutrients and soil pH and NH4+-N were the main factors regulating leaf- and root-related community composition, respectively. This study highlights that host selection effects on bacterial community structure were more prominent in aboveground than in belowground habitats. Our findings contribute to a better understanding of the effect of compartments and subtropical tree species on microbial diversity, with crucial implications for sustainable forest plantation management.

[Responses of arbuscular mycorrhizal fungi to elevated atmospheric CO2 concentration and warming: A review]. / ä¸›æžèŒæ ¹çœŸèŒå¯¹å¤§æ°”CO2æµ“åº¦å‡é«˜å’Œå¢žæ¸©å“åº”ç ”ç©¶è¿›å±•.

Global changes have profound impacts on biodiversity and ecological functioning of terrestrial ecosystems. Arbuscular mycorrhizal (AM) fungi can form symbiotic associations with most terrestrial plant species and play an important role in nutrient acquisition of host plants, promotion of plant growth, and maintenance of plant diversity. In this review, we primarily focused on the responses and feedbacks of AM fungal community and functioning to elevated atmospheric CO2(eCO2) and warming in forest and grassland ecosystems. eCO2 influenced AM fungi mainly through indirectly impacting host plants and soil carbon inputs. A majority of previous studies reported that eCO2 could enhance the abundance and activity of AM fungi, and influence their diversity and community composition. Warming could have direct and indirect (via plant and/or soil pathways) impacts on AM fungi. Warming significantly altered the community compositions of AM fungi in forest soils. But the results from grassland were not consistent. We identified some outstanding problems in current studies and proposed future research topics which deserve more attentions. Our aim was to elucidate the AM fungal responses and adaptation to eCO2 and warming and to improve our understanding of AM fungal functioning in soil ecological processes. This review could provide insights into the implications of AM fungi to mitigate global change and improve the resilience of soil functions, as well as climate change adaptation of ecosystems.

[Optimization for the determination of phenol oxidase activity in subtropical forest soils developed on sandstone]. / äºšçƒ­å¸¦ç ‚å²©å‘è‚²æ£®æž—åœŸå£¤é šæ°§åŒ–é ¶æ´»æ€§æµ‹å®šæ–¹æ³•ä¼˜åŒ–.

Phenol oxidase plays an important role in the degradation of soil organic matter. There was no standard method to determine soil phenol oxidase activity. To fill such knowledge gap, we investigated the effects of substrate type, pH, soil storage conditions, storage time, substrate concentration, water-soil ratio, incubation time and incubation temperature on soil phenol oxidase activity in three different subtropical forest soils developed on sandstone. The pH of extraction buffers significantly affected the phenol oxidase activity. Using 2,2'-azinobis-(-3-ethylbenzo-thiazoline-6-sulfononic acid)-diammonium salt (ABTS) as substrate acquired higher oxidase activity and was applicable to wider pH range than using 3-(3,4-Dihydroxyphenyl)-L-alanine (L-DOPA) as substrate, indicating that ABTS was more suitable as a substrate for measuring phenol oxidase activity in acidic soils of subtropical forests. The storage condition significantly affected phenol oxidase activity. The phenol oxidase activity declined with time in all the three types of soil. The decreasing rate was air-dried > 4 ℃ refrigerated > -20 ℃ frozen > -80 ℃ frozen, suggesting that the frozen storage method was better than others in maintaining soil phenol oxidase activity if the determination of phenol oxidase activity in fresh soil samples cannot be immediately done. Substrate concentration, water-soil ratio, and incubation time and temperature all affected the activity of soil phenol oxidase. The condition of soil: buffer ratio of 1:100, 2 mmol·L-1 concentration of ABTS with an incubation time of 4 h at 25-30 ℃ was optimal for measuring phenol oxidase activity in acidic soils of subtropical forests, with high repeatability and sensitivity.

Compartment and Plant Identity Shape Tree Mycobiome in a Subtropical Forest.

Deciphering the relationships between microbes and their host plants is critical for a better understanding of microbial diversity maintenance and community stability. Here, we investigated fungal diversity and community assembly in the phyllosphere and rhizosphere of 13 tree species in a subtropical common-garden experiment. The results showed that fungal community structures significantly differed across compartments (leaf, root, and soil) and different tree species. Higher α-diversity was observed in the phyllosphere than in the roots and rhizospheric soil. Fungal community composition (ß-diversity) was significantly affected by both compartment and species identity. The fungal community compositions were significantly correlated with soil pH in the roots and the soils as well as with soil nitrate and leaf total phosphorus in the leaves. We found that fungal community assemblies were mainly driven by deterministic processes, regardless of compartments. Moreover, host preference analyses indicated that stronger plant/fungus preferences occurred in leaves than in roots and soils. Our results highlight the differences in tree mycobiome between aboveground and belowground compartments and have important implications for the promotion of biodiversity conservation and management sustainability for the subtropical forest. IMPORTANCE Subtropical mountain forests are widely distributed in Southern China and are characterized by high biodiversity. The interactions between plants and fungi play pivotal roles in biodiversity maintenance and community stability. Nevertheless, knowledge of fungal diversity and of the community assembly patterns of woody plants is scarce. Here, we investigated fungal diversity and community assembly in the phyllosphere and rhizosphere of 13 tree species in a common-garden experiment. We found that both compartment and plant identity influenced fungal diversity, community, and guild compositions, while deterministic processes mainly governed the fungal community assembly, especially in the rhizospheric fungal communities. Our results demonstrate that tree leaves represent stronger host/fungi preferences than do roots and soils. Together, our findings enhance the understanding of the roles of compartment and plant identity in structuring fungal communities as well as promote fungal diversity maintenance in subtropical mountain forest ecosystems.

[Responses of soil microbial biomass and carbon source utilization to simulated nitrogen deposition and drought in a Cunninghamia lanceolata plantation]. / 杉木人工林土壤微生物生物量和碳源利用能力对模拟氮沉降和干旱的响应.

Chinese fir (Cunninghamia lanceolata) plantation is a dominant forest type and carbon sink in the subtropical region in China. An experiment with simulated nitrogen deposition (addition of 40 kg N·hm-2·a-1) and drought (50% of precipitation exclusion, PE) was established in Chinese fir plantation in 2018. Soil samples (0-15 cm) were collected in summer (July 2020) and winter (January 2021). Soil microbial biomass, colony forming units (CFUs) and carbon source utilization were determined through phospholipid fatty acids (PLFAs), plate count, and Biolog methods, respectively. The results showed significant seasonal variations of PLFAs-related microbial biomass and composition. Soil bacterial and fungal CFUs tended to be decreased by nitrogen addition or precipitation exclusion treatment, and bacterial CFUs were more sensitive to the two treatments than fungal CFUs. Soil microbial function (i.e. carbon source utilization) was not affected by nitrogen addition, but significantly decreased by precipitation exclusion. There was a significant positive correlation between bacterial CFUs and microbial function, indicating the crucial roles of culturable bacteria in microbial carbon transformation. Our results highlight the critical effects of nitrogen deposition and 50% reduced precipitation on microbes in topsoil of fir plantation, with implications for unraveling soil microbial ecological function of subtropical forest ecosystem under global changes in future.

[Long-term effects of imbalanced fertilization with lime and gypsum additions on denitrifying functional genes of an Ultisol at Yingtan, Jiangxi, China]. / é•¿æœŸç¼ºç´ æ–½è‚¥åŠçŸ³ç°çŸ³è†æ–½ç”¨å¯¹æ±Ÿè¥¿é¹°æ½­çº¢å£¤åç¡åŒ–å¾®ç”Ÿç‰©åŠŸèƒ½åŸºå› ä¸°åº¦çš„å½±å“.

The abundance of denitrifying functional genes plays a key role in driving the soil nitrous oxide (N2O) emission potential. Nitrite reductase genes (nirK and nirS) and nitrous oxide reductase genes (nosZ I and nosZ II) are the dominant denitrifying funtional genes. In this study, real-time quantitative PCR was conducted to evaluate the effects of 32-year imbalanced fertilization and lime and gypsum additions on the abundances of nirK, nirS, nosZ I and nosZ II genes in an Ultisol at Yingtan, Jiangxi Province. We further explored the underlying driving factors. The results showed that, compared with the balanced fertilization treatment, fertilization without phosphorus (P) signifi-cantly decreased the abundances of nirK, nirS, nosZ I and nosZ II genes. Fertilization without nitrogen (N) significantly reduced the abundances of nirK, nosZ I and nosZ II, but did not affect the abundance of nirS. Fertilization without potassium (K) did not affect the abundances of all denitri-fying functional genes. Results of stepwise regression analysis and random forest analysis showed that soil pH was a key environmental factor affecting the abundances of nosZ I and nosZ II. The application of lime or lime + gypsum significantly increased soil pH, which subsequently increased the abundances of nosZ II and nosZ II/nosZ I by 150%-231% and 127%-155%, respectively. Our results suggested that application of lime or lime + gypsum favored nosZ II more than nosZ I in upland Ultisols, which might enhance the relative importance of nosZ II in N2O reduction. Overall, fertilization without P would reduce denitrifying gene abundances, while the application of lime or lime + gypsum enriched nosZ II and increased ratio of nosZ II/nosZ I, which might be beneficial for reducing N2O emission potential in the Ultisols.

[Responses of denitrifying functional gene abundance to long-term fertilization regimes in an upland Ultisol]. / æ—±åœ°çº¢å£¤åç¡åŒ–åŠŸèƒ½åŸºå› ä¸°åº¦å¯¹é•¿æœŸæ–½è‚¥çš„å“åº”.

Fertilization affects soil nitrogen cycling and nitrous oxide (N2O) emissions, which are mainly driven by microbes. A 32-year field experiment was conducted to investigate the effects of chemical fertilizers and their combination with organic materials on the abundance of denitrifying functional genes (nirS, nirK, nosZ I and nosZ II) in Ultisol. The treatments comprised no fertilizer (CK), chemical fertilizer, chemical fertilizer+peanut straw, chemical fertilizer+rice straw, chemical fertilizer+radish and chemical fertilizer+pig manure. Compared with the single chemical fertilizer treatment, soil pH and organic carbon content increased in the chemical fertilizer plus organic material treatments, with chemical fertilizer+pig manure having the strongest effect. Long-term fertilization did not affect the abundance of nirK gene, but significantly altered the nirS gene abundance. Compared to CK, long-term chemical fertilizer application increased the abundance of nirS gene by 426%. However, partial replacement of chemical fertilizer by organic materials decreased the abundance of nirS gene. The abundance of nosZ I gene was one order of magnitude higher than that of nosZ II, indicating the domination of nosZ I in the acidic Ultisol. Long-term fertilization did not affect the abundance of nosZ II, whereas chemical fertilizer+pig manure increased the abundance of nosZ I by 138%. Results of stepwise regression analysis showed that available phosphorus content was the primary factor regulating the abundance of nosZ I gene, whereas the abundance of the nosZ II gene was mainly regulated by nitrate content. Moreover, the lowest (nirS+nirK)/(nosZ I+nosZ II) value in the chemical fertilizer+pig manure treatment indicated that long-term manure application might reduce N2O emission potential in Ultisols.