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1.
Phytopathology ; 114(7): 1612-1625, 2024 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-38478699

RESUMEN

Unraveling the intricacies of soybean cyst nematode (Heterodera glycines) race 4 resistance and susceptibility in soybean breeding lines-11-452 (highly resistant) and Dongsheng1 (DS1, highly susceptible)-was the focal point of this study. Employing cutting-edge N6-methyladenosine (m6A) and RNA sequencing techniques, we delved into the impact of m6A modification on gene expression and plant defense responses. Through the evaluation of nematode development in both resistant and susceptible roots, a pivotal time point (3 days postinoculation) for m6A methylation sequencing was identified. Our sequencing data exhibited robust statistics, successful soybean genome mapping, and prevalent m6A peak distributions, primarily in the 3' untranslated region and stop codon regions. Analysis of differential methylation peaks and differentially expressed genes revealed distinctive patterns between resistant and susceptible genotypes. In the highly resistant line (11-452), key resistance and defense-associated genes displayed increased expression coupled with inhibited methylation, encompassing crucial players such as R genes, receptor kinases, and transcription factors. Conversely, the highly susceptible DS1 line exhibited heightened expression correlated with decreased methylation in genes linked to susceptibility pathways, including Mildew Locus O-like proteins and regulatory elements affecting defense mechanisms. Genome-wide assessments, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses, and differential methylation peak/differentially expressed gene overlap emphasized the intricate interplay of m6A modifications, alternative splicing, microRNA, and gene regulation in plant defense. Protein-protein interaction networks illuminated defense-pivotal genes, delineating divergent mechanisms in resistant and susceptible responses. This study sheds light on the dynamic correlation between methylation, splicing, and gene expression, providing profound insights into plant responses to nematode infection.


Asunto(s)
Adenosina , Glycine max , Enfermedades de las Plantas , Tylenchoidea , Glycine max/genética , Glycine max/parasitología , Glycine max/inmunología , Tylenchoidea/fisiología , Enfermedades de las Plantas/parasitología , Enfermedades de las Plantas/inmunología , Enfermedades de las Plantas/genética , Adenosina/análogos & derivados , Adenosina/metabolismo , Animales , Metilación , Resistencia a la Enfermedad/genética , Regulación de la Expresión Génica de las Plantas , Análisis de Secuencia de ARN , Raíces de Plantas/parasitología , Raíces de Plantas/genética , Raíces de Plantas/inmunología
2.
Ying Yong Sheng Tai Xue Bao ; 24(2): 351-8, 2013 Feb.
Artículo en Zh | MEDLINE | ID: mdl-23705378

RESUMEN

Three light intensities (100% , 56.2%, and 12.5%) were installed to simulate the light regimes of opening field (cutting blank), forest gap, and understory, respectively, aimed to understand the effects of different light regimes on the seedling growth, photosynthetic characteristics, and biomass accumulation and allocation of alien species Alnus formosana and native species A. cremastogyne. Low light regime limited the seedling growth of the two alder species, while the light regime of forest gap was more favorable for the growth, in comparison with that of the opening field. Regardless of the light regimes, A. formosana seedlings had higher specific leaf area (SLA), relative growth rate (RGR) , leaf area, leaf length, leaf width, plant height, and basal diameter, but smaller leaf number, leaf area ratio (LAR), and petiole length. Under low light regime, A. formosana seedlings had higher maximum net photosynthetic rate (Pn max), light saturation point (LSP), and apparent quantum yield (AQY), but smaller light compensation point (LCP) and dark respiration rate (Rday). With the decrease of light intensity, A. formosana seedlings had much higher root mass ratio (RMR) and much lower leaf mass ratio (LMR), implying that more carbon was allocated and stored to the roots rather than new leaves, whereas the A. cremastogyne seedlings were in adverse, i.e. , more carbon was allocated to the above-ground parts, which might increase the risk of animal feeding and mechanical damage.


Asunto(s)
Alnus/crecimiento & desarrollo , Alnus/fisiología , Luz , Fotosíntesis/fisiología , Plantones , Alnus/clasificación , Biomasa , China , Hojas de la Planta/fisiología , Plantones/crecimiento & desarrollo , Plantones/fisiología
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