Weather explains the decline and rise of insect biomass over 34 years.

Insects have a pivotal role in ecosystem function, thus the decline of more than 75% in insect biomass in protected areas over recent decades in Central Europe1 and elsewhere2,3 has alarmed the public, pushed decision-makers4 and stimulated research on insect population trends. However, the drivers of this decline are still not well understood. Here, we reanalysed 27 years of insect biomass data from Hallmann et al.1, using sample-specific information on weather conditions during sampling and weather anomalies during the insect life cycle. This model explained variation in temporal decline in insect biomass, including an observed increase in biomass in recent years, solely on the basis of these weather variables. Our finding that terrestrial insect biomass is largely driven by complex weather conditions challenges previous assumptions that climate change is more critical in the tropics5,6 or that negative consequences in the temperate zone might only occur in the future7. Despite the recent observed increase in biomass, new combinations of unfavourable multi-annual weather conditions might be expected to further threaten insect populations under continuing climate change. Our findings also highlight the need for more climate change research on physiological mechanisms affected by annual weather conditions and anomalies.

Gregarines modulate insect responses to sublethal insecticide residues.

Throughout their lifetime, insects face multiple environmental challenges that influence their performance. Gregarines are prevalent endoparasites in most invertebrates that affect the fitness of their hosts, but are often overlooked in ecological studies. Next to such biotic factors, a current common challenge is anthropogenic pollution with pesticides, which causes a major threat to non-target organisms that are readily exposed to lethal or sublethal concentrations. In a laboratory study, we investigated whether the presence of gregarines modulates the food consumption and life history traits of a (non-target) leaf beetle species, Phaedon cochleariae, in response to sublethal insecticide exposure. We show that the larval food consumption of the herbivore was neither affected by gregarine infection nor sublethal insecticide exposure. Nevertheless, infection with gregarines led to a delayed development, while insecticide exposure resulted in a lower body mass of adult males and a reduced reproduction of females. Individuals exposed to both challenges suffered most, as they had the lowest survival probability. This indicates detrimental effects on the population dynamics of non-target insects infected with naturally occurring gregarines that face additional stress from agrochemical pollution. Moreover, we found that the infection load with gregarines was higher in individuals exposed to sublethal insecticide concentrations compared to unexposed individuals. To counteract the global decline of insects, the potential of natural parasite infections in modulating insect responses to anthropogenic and non-anthropogenic environmental factors should be considered in ecological risk assessment.

Direct and delayed effects of exposure to a sublethal concentration of the insecticide λ-cyhalothrin on food consumption and reproduction of a leaf beetle.

Anthropogenic pollution such as the application of pesticides poses a major threat to many (non-target) organisms. However, little is known about the persistence of harmful effects or potential recovery in response to a period of exposure to a sublethal insecticide dose. Adults of the mustard leaf beetle, Phaedon cochleariae (Coleoptera: Chrysomelidae), were either exposed to a sublethal concentration of the pyrethroid λ-cyhalothrin for two weeks or kept unexposed as control. During, immediately after and at a delayed time after the exposure, consumption and reproduction, i.e., number of eggs laid and hatching success, were assessed. In addition, long-term effects on unexposed offspring were investigated. Exposure to λ-cyhalothrin reduced the consumption during the insecticide exposure, but led to compensatory feeding in females at a delayed time after exposure. The reproductive output of females was impaired during and directly after λ-cyhalothrin exposure. At the delayed time point there was no clear evidence for a recovery, as the reproduction of heavier females was still negatively affected, while lighter females showed an enhanced reproduction. Persistent negative effects on unexposed offspring had been found when collected from parents directly after a λ-cyhalothrin exposure period. In contrast, in the present experiment neither negative effects on life-history traits nor on consumption were observed in unexposed offspring derived from parents at the delayed time after λ-cyhalothrin exposure. Moreover, eggs of offspring from insecticide-exposed parents showed a higher hatching success than those of offspring of unexposed parents, which may indicate transgenerational hormesis. Our results highlight that λ-cyhalothrin exposure has persistent negative effects on fitness parameters of the exposed generation. However, offspring may not be harmed if their parents had sufficient time to recover after such an insecticide exposure. Taken together, our study emphasises that the time-course of exposure to this anthropogenic pollution is crucial when determining the consequences on life-history.

Dispersal and life-history traits in a spider with rapid range expansion.

BACKGROUND: Dispersal and reproduction are key life-history traits that jointly determine species' potential to expand their distribution, for instance in light of ongoing climate change. These life-history traits are known to be under selection by changing local environmental conditions, but they may also evolve by spatial sorting. While local natural selection and spatial sorting are mainly studied in model organisms, we do not know the degree to which these processes are relevant in the wild, despite their importance to a comprehensive understanding of species' resistance and tolerance to climate change. METHODS: The wasp spider Argiope bruennichi has undergone a natural range expansion - from the Mediterranean to Northern Europe during the recent decades. Using reciprocal common garden experiments in the laboratory, we studied differences in crucial traits between replicated core (Southern France) and edge (Baltic States) populations. We tested theoretical predictions of enhanced dispersal (ballooning behaviour) and reproductive performance (fecundity and winter survival) at the expansion front due to spatial sorting and local environmental conditions. RESULTS: Dispersal rates were not consistently higher at the northern expansion front, but were impacted by the overwintering climatic conditions experienced, such that dispersal was higher when spiderlings had experienced winter conditions as occur in their region. Hatching success and winter survival were lower at the range border. In agreement with theoretical predictions, spiders from the northern leading edge invested more in reproduction for their given body size. CONCLUSIONS: We found no evidence for spatial sorting leading to higher dispersal in northern range edge populations of A. bruennichi. However, reproductive investment and overwintering survival between core and edge populations differed. These life-history traits that directly affect species' expansion rates seem to have diverged during the recent range expansion of A. bruennichi. We discuss the observed changes with respect to the species' natural history and the ecological drivers associated with range expansion to northern latitudes.