RESUMEN
Proteomic studies in unicellular eukaryotes identified a set of centriolar proteins that included proteome of centriole 1 (Poc1). Functional studies in these organisms implicated Poc1 in centriole duplication and length control, as well as ciliogenesis. Using isoform-specific antibodies and RNAi depletion, we have examined the function of the two related human proteins, Poc1A and Poc1B. We find that Poc1A and Poc1B each localize to centrioles and spindle poles, but do so independently and with different dynamics. However, although loss of one or other Poc1 protein does not obviously disrupt mitosis, depletion of both proteins leads to defects in spindle organization with the generation of unequal or monopolar spindles. Our data indicate that, once incorporated, a fraction of Poc1A and Poc1B remains stably associated with parental centrioles, but that depletion prevents incorporation into nascent centrioles. Nascent centrioles lacking both Poc1A and Poc1B exhibit loss of integrity and maturation, and fail to undergo duplication. Thus, when Poc1A and Poc1B are co-depleted, new centrosomes capable of maturation cannot assemble and unequal spindles result. Interestingly, Poc1B, but not Poc1A, is phosphorylated in mitosis, and depletion of Poc1B alone was sufficient to perturb cell proliferation. Hence, Poc1A and Poc1B play redundant, but essential, roles in generation of stable centrioles, but Poc1B may have additional independent functions during cell cycle progression.
Asunto(s)
Centriolos/metabolismo , Proteínas/metabolismo , Huso Acromático/metabolismo , Western Blotting , Ciclo Celular/genética , Ciclo Celular/fisiología , Proteínas de Ciclo Celular , Línea Celular , Línea Celular Tumoral , Proteínas del Citoesqueleto , Electroforesis en Gel de Poliacrilamida , Citometría de Flujo , Células HeLa , Humanos , Inmunoprecipitación , Mitosis/genética , Mitosis/fisiología , Proteínas/genéticaRESUMEN
Embryonic development combines paradoxical properties: it has great precision, it is usually conducted at breakneck speed and it is flexible on relatively short evolutionary time scales, particularly at early stages. While these features appear mutually exclusive, we consider how they may be reconciled by the properties of key early regulatory networks. We illustrate these ideas with the network that controls development of endoderm progenitors. We argue that this network enables precision because of its intrinsic stability, self propagation and dependence on signalling. The network enables high developmental speed because it is rapidly established by maternal inputs at multiple points. In turn these properties confer flexibility on an evolutionary time scale because they can be initiated in many ways, while buffering essential progenitor cell populations against changes in their embryonic environment on both evolutionary and developmental time scales. Although stable, these networks must be capable of rapid dissolution as cell differentiation progresses. While we focus on the core early endodermal network of vertebrates, we argue that these properties are likely to be general in early embryonic stem cell populations, such as mammalian ES cells.
Asunto(s)
Endodermo/metabolismo , Regulación del Desarrollo de la Expresión Génica , Animales , Diferenciación Celular , Biología Evolutiva/métodos , Células Madre Embrionarias/citología , Evolución Molecular , Femenino , Humanos , Masculino , Ratones , Modelos Biológicos , Modelos Teóricos , Transducción de Señal , VertebradosRESUMEN
A microarray analysis of vegetal pole sequences in the egg and early Xenopus laevis embryo identified Unigene Xl.14891 as a vegetally localized RNA. Analysis of the Xenopus tropicalis genome showed this Unigene to be localized near the 3' end of the Grip2 (glutamate receptor interacting protein 2) transcription unit. RACE showed that the Unigene represented the 3' UTR of Grip2 mRNA. Grip2 mRNA is present in the mitochondrial cloud of late pre-vitellogenic oocytes and then in the germplasm through oogenesis and early development until tailbud tadpole stages. Interference with Grip2 mRNA translation using two antisense morpholino oligos (MOs) impairs primordial germ cell (PGC) migration to the germinal ridges. Both MOs also inhibit swimming movements of the tailbud tadpole, known to involve glutamate receptors. We conclude that Grip2 has several functions in the embryo, including enabling efficient PGC migration.
Asunto(s)
Proteínas Portadoras/genética , Movimiento Celular , Células Germinativas/citología , Proteínas de Xenopus/genética , Animales , Secuencia de Bases , Clonación Molecular , Cartilla de ADN , Hibridación in Situ , Péptidos y Proteínas de Señalización Intracelular , Oligonucleótidos Antisentido/genética , Sistemas de Lectura Abierta , ARN Mensajero/genética , Xenopus laevisRESUMEN
The evolution of multicellular animals has been attributed to many kinds of selective advantage; here I suggest that the evolution of somatic cells to feed and protect the germline was central to the appearance of animals. This would have been driven by selection for extreme anisogamy--the evolution of sperm and egg. Evidence is adduced from the germline stem cells of simple animals (defining germline as any cell that normally produces the next generation via the sexual process) and from the control circuitry ubiquitous in animal germlines. With the soma and its elaboration came animal development, as we understand it.
Asunto(s)
Evolución Biológica , Células Germinativas/citología , Reproducción/fisiología , AnimalesRESUMEN
We have used antisense morpholino oligos to establish the developmental roles of three Xsox17 proteins in Xenopus development (Xsox17alpha(1), alpha(2) and beta). We show that their synthesis can be inhibited with modest amounts of oligo. The inhibition of each individually produces defects in late midgut development. Loss of activity of the Xsox17alpha proteins additionally inhibits hindgut formation, and inhibiting Xsox17alpha(1) disrupts foregut development with variable penetrance. When all Xsox17 activity is inhibited cell movements are halted during late gastrulation and the transcription of several endodermally expressed genes is reduced. Thus the Xsox17 proteins have redundant roles in early development of the endoderm and partly distinct roles during later organogenesis.
Asunto(s)
Endodermo/fisiología , Proteínas del Grupo de Alta Movilidad , Proteínas/genética , Proteínas de Xenopus/genética , Xenopus/embriología , Regiones no Traducidas 5' , Animales , Movimiento Celular/genética , Proteínas de Unión al ADN/genética , Sistema Digestivo/embriología , Anomalías del Sistema Digestivo , Embrión no Mamífero , Regulación del Desarrollo de la Expresión Génica/efectos de los fármacos , Factor Nuclear 1-beta del Hepatocito , Técnicas In Vitro , Larva , Familia de Multigenes , Oligonucleótidos Antisentido/farmacología , Fenotipo , Biosíntesis de Proteínas , Proteínas/metabolismo , Factores de Transcripción SOXF , Factores de Transcripción/efectos de los fármacos , Factores de Transcripción/genética , Xenopus/genética , Proteínas de Xenopus/efectos de los fármacos , Proteínas de Xenopus/metabolismoRESUMEN
In Xenopus laevis, the change in the amount of histone H4 mRNA per embryo measured by Northern blotting methods follows a unique change during early embryogenesis: It starts to increase first at the blastula stage, doubles by the gastrula stage then decreases considerably at the neurula stage, and then increases again from the tailbud stage on. The present paper establishes these developmental changes, and furthermore, provides evidence that the synthesis of H4 mRNA starts or at least increases to a detectable level at the midblastula stage as shown by S-1 protection analysis of the expression of paternal histone H4 genes in X. borealis (â) and X. laevis (â) hybrid embryos.
RESUMEN
Hermes is an RNA-binding protein that we have previously reported to be found in the ribonucleoprotein (RNP) particles of Xenopus germ plasm, where it is associated with various RNAs, including that encoding the germ line determinant Nanos1. To further define the composition of these RNPs, we performed a screen for Hermes-binding partners using the yeast two-hybrid system. We have identified and validated four proteins that interact with Hermes in germ plasm: two isoforms of Xvelo1 (a homologue of zebrafish Bucky ball) and Rbm24b and Rbm42b, both RNA-binding proteins containing the RRM motif. GFP-Xvelo fusion proteins and their endogenous counterparts, identified with antisera, were found to localize with Hermes in the germ plasm particles of large oocytes and eggs. Only the larger Xvelo isoform was naturally found in the Balbiani body of previtellogenic oocytes. Bimolecular fluorescence complementation (BiFC) experiments confirmed that Hermes and the Xvelo variants interact in germ plasm, as do Rbm24b and 42b. Depletion of the shorter Xvelo variant with antisense oligonucleotides caused a decrease in the size of germ plasm aggregates and loosening of associated mitochondria from these structures. This suggests that the short Xvelo variant, or less likely its RNA, has a role in organizing and maintaining the integrity of germ plasm in Xenopus oocytes. While GFP fusion proteins for Rbm24b and 42b did not localize into germ plasm as specifically as Hermes or Xvelo, BiFC analysis indicated that both interact with Hermes in germ plasm RNPs. They are very stable in the face of RNA depletion, but additive effects of combinations of antisense oligos suggest they may have a role in germ plasm structure and may influence the ability of Hermes protein to effectively enter RNP particles.
Asunto(s)
Ribonucleoproteínas/metabolismo , Proteínas de Xenopus/metabolismo , Xenopus/metabolismo , Animales , Proteínas Portadoras/metabolismo , Femenino , Células Germinativas/metabolismo , Mutación , Oligodesoxirribonucleótidos Antisentido/genética , Oligodesoxirribonucleótidos Antisentido/metabolismo , Oocitos/metabolismo , Unión Proteica , Dominios y Motivos de Interacción de Proteínas , Mapeo de Interacción de Proteínas , Transporte de Proteínas , Ribonucleoproteínas/química , Ribonucleoproteínas/genética , Técnicas del Sistema de Dos Híbridos , Proteínas de Xenopus/química , Proteínas de Xenopus/genéticaRESUMEN
We have studied the localisation of mRNAs in full-grown Xenopus laevis oocytes by injecting fluorescent RNAs, followed by confocal microscopy of the oocyte cortex. Concentrating on RNA encoding the Xenopus Nanos homologue, nanos1 (formerly Xcat2), we find that it consistently localised into aggregated germ plasm ribonucleoprotein (RNP) particles, independently of cytoskeletal integrity. This implies that a diffusion/entrapment-mediated mechanism is active, as previously reported for previtellogenic oocytes. Sometimes this was accompanied by localisation into scattered particles of the "late", Vg1/VegT pathway; occasionally only late pathway localisation was seen. The Xpat RNA behaved in an identical fashion and for neither RNA was the localisation changed by any culture conditions tested. The identity of the labelled RNP aggregates as definitive germ plasm was confirmed by their inclusion of abundant mitochondria and co-localisation with the germ plasm protein Hermes. Further, the nanos1/Hermes RNP particles are interspersed with those containing the germ plasm protein Xpat. These aggregates may be followed into the germ plasm of unfertilized eggs, but with a notable reduction in its quantity, both in terms of injected molecules and endogenous structures. Our results conflict with previous reports that there is no RNA localisation in large oocytes, and that during mid-oogenesis even germ plasm RNAs localise exclusively by the late pathway. We find that in mid oogenesis nanos1 RNA also localises to germ plasm but also by the late pathway. Late pathway RNAs, Vg1 and VegT, also may localise into germ plasm. Our results support the view that mechanistically the two modes of localisation are extremely similar, and that in an injection experiment RNAs might utilise either pathway, the distinction in fates being very subtle and subject to variation. We discuss these results in relation to their biological significance and the results of others.
Asunto(s)
Regulación del Desarrollo de la Expresión Génica , Oocitos/metabolismo , ARN Mensajero/metabolismo , Vitelogénesis/genética , Xenopus laevis/metabolismo , Animales , Femenino , Colorantes Fluorescentes , Microinyecciones , Microscopía Confocal , Microscopía Fluorescente , Oocitos/crecimiento & desarrollo , Oocitos/ultraestructura , ARN Mensajero/genética , Proteínas de Unión al ARN/genética , Proteínas de Unión al ARN/metabolismo , Proteínas Represoras/genética , Proteínas Represoras/metabolismo , Ribonucleoproteínas/genética , Ribonucleoproteínas/metabolismo , Ribonucleoproteínas/ultraestructura , Transducción de Señal , Proteínas de Dominio T Box/genética , Proteínas de Dominio T Box/metabolismo , Factores de Tiempo , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , Factor de Crecimiento Transformador beta/genética , Factor de Crecimiento Transformador beta/metabolismo , Proteínas de Xenopus/genética , Proteínas de Xenopus/metabolismo , Xenopus laevis/crecimiento & desarrolloRESUMEN
We have made a wide phylogenetic survey of Pix proteins, which are constituents of vertebrate centrioles in most eukaryotes. We have also surveyed the presence and structure of flagella or cilia and centrioles in these organisms, as far as is possible from published information. We find that Pix proteins are present in a vast range of eukaryotes, but not all. Where centrioles are absent so are Pix proteins. If one considers the maintenance of Pix proteins over evolutionary time scales, our analysis would suggest that their key function is to make cilia and flagella, and the same is true of centrioles. Moreover, this survey raises the possibility that Pix proteins are only maintained to make cilia and flagella that undulate, and even then only when they are constructed by transporting ciliary constituents up the cilium using the intraflagellar transport (IFT) system. We also find that Pix proteins have become generally divergent within Ecdysozoa and between this group and other taxa. This correlates with a simplification of centrioles within Ecdysozoa and a loss or divergence of cilia/flagella. Thus Pix proteins act as a weathervane to indicate changes in centriole function, whose core activity is to make cilia and flagella.
Asunto(s)
Centriolos/genética , Factores de Intercambio de Guanina Nucleótido/metabolismo , Secuencia de Aminoácidos , Animales , Centriolos/metabolismo , Centriolos/ultraestructura , Chlamydomonas/fisiología , Drosophila melanogaster/fisiología , Flagelos/metabolismo , Modelos Biológicos , Conformación Molecular , Datos de Secuencia Molecular , Filogenia , Estructura Terciaria de Proteína , Factores de Intercambio de Guanina Nucleótido Rho , Homología de Secuencia de Aminoácido , Huso Acromático , Xenopus laevisRESUMEN
In many animals, the germ line develops from a distinct mitochondria-rich region of embryonic cytoplasm called the germ plasm. However, the protein composition of germ plasm and its formation remain poorly understood, except in Drosophila. Here, we show that Xpat, a recently identified protein component of Xenopus germ plasm, interacts via its C-terminal domain with a novel protein, xPix1. Xpat and xPix1 are co-expressed in ovaries, eggs and early embryos and colocalize to the mitochondrial cloud and germ plasm in stage I and stage VI oocytes, respectively. Although Xpat appears unique to Xenopus, Pix proteins, which contain an N-terminal WD40 domain and C-terminal coiled-coil, are widely conserved. In humans, two proteins, Pix1 and Pix2, are expressed at varying levels in different cancer cell lines. Importantly, as well as localizing to mitochondria, human Pix proteins localize to centrosomes and associate with microtubules in vitro and in vivo. Although, Pix proteins are stably expressed through the cell cycle, Pix2 concentrates on microtubule structures in mitosis and microinjection of Pix antibodies interferes with cell division. Based on these data, we propose that Pix1 and Pix2 are microtubule-associated adaptor proteins that likely contribute to a range of developmental and cell division processes.
Asunto(s)
Centrosoma/metabolismo , Citoplasma/metabolismo , Proteínas Asociadas a Microtúbulos/metabolismo , Oocitos/metabolismo , Plaquinas/metabolismo , Xenopus laevis/metabolismo , Animales , Células COS , División Celular/fisiología , Línea Celular , Centrosoma/ultraestructura , Chlorocebus aethiops , Secuencia Conservada , Citoplasma/genética , Citoplasma/ultraestructura , Femenino , Células HeLa , Humanos , Proteínas Asociadas a Microtúbulos/genética , Proteínas Asociadas a Microtúbulos/aislamiento & purificación , Microtúbulos/metabolismo , Microtúbulos/ultraestructura , Mitocondrias/metabolismo , Mitocondrias/ultraestructura , Datos de Secuencia Molecular , Oocitos/ultraestructura , Plaquinas/genética , Plaquinas/aislamiento & purificación , Estructura Terciaria de Proteína/fisiología , Homología de Secuencia de Aminoácido , Homología de Secuencia de Ácido Nucleico , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , Técnicas del Sistema de Dos Híbridos , Proteínas de Xenopus/genética , Proteínas de Xenopus/aislamiento & purificación , Proteínas de Xenopus/metabolismo , Xenopus laevis/genéticaRESUMEN
The gene encoding the Sox F-group transcription factor Xsox17alpha(1) is specifically expressed throughout the entire region of the Xenopus blastula fated to become endoderm, and is important in controlling endodermal development. Xsox17alpha(1) is a direct target of the maternal endodermal determinant VegT and of Sox17 itself. We have analysed the promoter of the Xenopus laevis Xsox17alpha(1) gene by transgenesis, and have identified two important control elements which reside about 9 kb upstream at the start of transcription. These elements individually drive transgenic endodermal expression in the blastula and gastrula. One contains functional, cooperating VegT and Sox-binding consensus sites. The Sox sites in this region are occupied in vivo. The other responds to TGF-beta signals like Activin or Nodals that act through Smad2/3. We propose that these two regions co-operate in regulating the early endodermal expression of the Xsox17alpha(1) gene.
Asunto(s)
Proteínas de Unión al ADN/metabolismo , Regulación del Desarrollo de la Expresión Génica , Proteínas del Grupo de Alta Movilidad/metabolismo , Regiones Promotoras Genéticas , Proteínas de Dominio T Box/metabolismo , Factores de Transcripción/metabolismo , Proteínas de Xenopus/metabolismo , Xenopus laevis/embriología , Animales , Blástula/embriología , Blástula/metabolismo , Proteínas de Unión al ADN/genética , Endodermo/embriología , Endodermo/metabolismo , Proteínas del Grupo de Alta Movilidad/genética , Mutación , Factores de Transcripción SOXF , Proteínas de Dominio T Box/genética , Factores de Transcripción/genética , Proteínas de Xenopus/genética , Xenopus laevis/metabolismoRESUMEN
Germ plasm plays a prominent role in germline formation in a large number of animal taxons. We previously identified a novel maternal RNA named Germes associated with Xenopus germ plasm. In the present work, we addressed possible involvement of Germes protein in germ plasm function. Expression in oocytes followed by confocal microscopy revealed that the EGFP fused to Germes, in contrast to the free EGFP, co-localized with the germ plasm. Overexpression of intact Germes and Germes lacking both leucine zipper motifs (GermesDeltaLZs) resulted in a statistically significant reduction of the number of primordial germ cells (PGCs). Furthermore, the GermesDeltaLZs mutant inhibited PGC migration and produced abnormalities in germ plasm intra-cellular distribution at tailbud stages. To begin unraveling biochemical interactions of Germes during embryogenesis, we searched for Germes partners using yeast two-hybrid (YTH) system. Two closely related sequences were identified, encoding Xenopus dynein light chains dlc8a and dlc8b. Tagged versions of Germes and dlc8s co-localize in VERO cells upon transient expression and can be co-immunoprecipitated after injection of the corresponding RNAs in Xenopus embryos, indicating that their interactions occur in vivo. We conclude that Germes is involved in organization and functioning of germ plasm in Xenopus, probably through interaction with motor complexes.
Asunto(s)
Proteínas Portadoras/metabolismo , Proteínas de Drosophila/metabolismo , Células Germinativas/metabolismo , ARN Mensajero/metabolismo , Proteínas de Unión al ARN/fisiología , Proteínas de Xenopus/fisiología , Xenopus/embriología , Animales , Proliferación Celular , Células Cultivadas , Clonación Molecular , Dineínas , Femenino , Colorantes Fluorescentes/análisis , Células Germinativas/citología , Células Germinativas/crecimiento & desarrollo , Proteínas Fluorescentes Verdes/análisis , Mutación , Oocitos/crecimiento & desarrollo , Oocitos/metabolismo , Proteínas de Unión al ARN/genética , Regulación hacia Arriba , Xenopus/crecimiento & desarrollo , Proteínas de Xenopus/genéticaRESUMEN
A conserved molecular pathway has emerged controlling endoderm formation in Xenopus zebrafish and mice. Key genes in this pathway include Nodal ligands and transcription factors of the Mix-like paired homeodomain class, Gata4-6 zinc-finger factors and Sox17 HMG domain proteins. Although a linear epistatic pathway has been proposed, the precise hierarchical relationships between these factors and their downstream targets are largely unresolved. Here, we have used a combination of microarray analysis and loss-of-function experiments to examine the global regulatory network controlling Xenopus endoderm formation. We identified over 300 transcripts enriched in the gastrula endoderm, including most of the known endoderm regulators and over a hundred uncharacterized genes. Surprisingly only 10% of the endoderm transcriptome is regulated as predicted by the current linear model. We find that Nodal genes, Mixer and Sox17 have both shared and distinct sets of downstream targets, and that a number of unexpected autoregulatory loops exist between Sox17 and Gata4-6, between Sox17 and Bix1/Bix2/Bix4, and between Sox17 and Xnr4. Furthermore, we find that Mixer does not function primarily via Sox17 as previously proposed. These data provides new insight into the complexity of endoderm formation and will serve as valuable resource for establishing a complete endoderm gene regulatory network.
Asunto(s)
Endodermo/citología , Factores de Transcripción/metabolismo , Transcripción Genética , Xenopus/embriología , Xenopus/metabolismo , Animales , Embrión no Mamífero , Endodermo/metabolismo , Gástrula , Regulación del Desarrollo de la Expresión Génica , Hibridación in Situ , Microinyecciones , Análisis de Secuencia por Matrices de Oligonucleótidos , Oligonucleótidos Antisentido/farmacología , Técnicas de Cultivo de Órganos , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Factores de Transcripción/genética , Xenopus/genética , Proteínas de Xenopus/genética , Proteínas de Xenopus/metabolismoRESUMEN
In many animals, including Drosophila, C. elegans, zebrafish and Xenopus, the germ line is specified by maternal determinants localised in a distinct cytoplasmic structure called the germ plasm. This is consists of dense granules, mitochondria, and specific localised RNAs. We have characterised the expression and properties of the protein encoded by Xpat, an RNA localised to the germ plasm of Xenopus. Immunofluorescence and immunoblotting showed that this novel protein is itself a major constituent of germ plasm throughout oogenesis and early development, although it is also present in other regions of oocytes and embryos, including their nuclei. We found that an Xpat-GFP fusion protein can localise correctly in cultured oocytes, in early oocytes to the 'mitochondrial cloud', from which germ plasm originates, and in later oocytes to the vegetal cortex. The localisation process was microtubule-dependent, while cortical anchoring required microfilaments. Xpat-GFP expressed in late stage oocytes assembled into circular fields of multi-particulate structures resembling endogenous fields of germ plasm islands. Furthermore these structures could be induced to form at ectopic sites by manipulation of culture conditions. Ectopic Xpat-GFP islands were able to recruit mitochondria, a major germ plasm component. These data suggest that Xpat protein has an important role in Xenopus germ plasm formation, positioning and maintenance.
Asunto(s)
Citoplasma/metabolismo , Oocitos/metabolismo , Factores de Transcripción/fisiología , Proteínas de Xenopus/fisiología , Xenopus/fisiología , Citoesqueleto de Actina/fisiología , Animales , Núcleo Celular/metabolismo , Microtúbulos/fisiología , Mitocondrias/metabolismo , Oogénesis/fisiología , Transporte de Proteínas , ARN Mensajero/biosíntesis , Factores de Transcripción/biosíntesis , Factores de Transcripción/genética , Xenopus/metabolismo , Proteínas de Xenopus/biosíntesis , Proteínas de Xenopus/genéticaRESUMEN
The maternal determinant VegT is required for both endoderm and mesoderm formation by the Xenopus embryo. An important downstream mediator of VegT action is Xsox17, which has been proposed to be induced in cell-autonomous, then signal-dependent phases. We show that Xsox17 is a direct VegT target, but that direct induction of Xsox17 by VegT is rapidly inhibited. This inhibition is relieved by TGF- beta signalling, to which the future endoderm cell is sensitised by VegT, resulting in the observed dependence on cell contact for maintained Xsox17 expression. We propose that this change in regulation is a consequence of a VegT-induced repressor, inhibiting direct induction of early endoderm markers by VegT, and contributing to the formation of the boundary of the endodermal domain.
Asunto(s)
Proteínas de Unión al ADN , Proteínas del Grupo de Alta Movilidad , Proteínas de Dominio T Box/fisiología , Factores de Transcripción , Factor de Crecimiento Transformador beta/fisiología , Proteínas de Xenopus , Xenopus/embriología , Animales , Inducción Embrionaria , Endodermo/citología , Endodermo/metabolismo , Femenino , Regulación del Desarrollo de la Expresión Génica , Hibridación in Situ , Mesodermo/citología , Mesodermo/metabolismo , Modelos Biológicos , Proteínas/genética , Proteínas/fisiología , Factores de Transcripción SOXF , Transducción de Señal , Proteínas de Dominio T Box/genética , Xenopus/genética , Xenopus/fisiologíaRESUMEN
We have raised a monoclonal antibody, 4G6, against gut manually isolated from stage 42Xenopus laevis embryos. It is specific for endoderm and recognises an epitope that is first expressed at stage 19 and which persists throughout subsequent development. The antibody maintains gut specificity through metamorphosis and into adulthood. The epitope is conserved in the mouse, where it is also found in the gut. Isolated vegetal poles fromXenopus blastula stage embryos express the epitope autonomously after culturing to the appropriate stage. This shows that certain aspects of endoderm differentiation do not require germ layer interactions. Animal cap cells from stage 9 blastulae cultured in the presence of the mesodermal growth factors FGF, XTC-MIF and PIF form both endodermal and mesodermal tissues, assessed by the binding of tissue-specific monoclonal antibodies. Endoderm is typically found in those caps which form intermediate and ventral forms of mesoderm, that is muscle and lateral plate.
RESUMEN
The Endodermin gene is expressed in the early endoderm and the Spemann organizer of Xenopus embryos. It has previously been shown to be a direct target of the early endodermal transcription factor Xsox17 (Clements et al., 2003, Mech Dev 120:337-348). Here we identify two adjacent control elements in the Endodermin promoter; these drive transcription of the gene in late-gastrula endoderm and contain consensus Sox-binding sites. We have analyzed one element in detail and show that it responds directly to Xsox17 and that the Sox sites are essential for endodermal expression in transgenic embryos. However, flanking regions on both sides are also essential, indicating that Xsox17 acts in concert with several DNA-binding partners.