Detunable wireless Litzcage coil for human head MRI at 1.5 T.

Inductively coupled radiofrequency (RF) coils are an inexpensive and simple method to realize wireless RF coils in magnetic resonance imaging (MRI), which can significantly ease the MRI scan setup and improve patient comfort because they do not require bulky components such as cables, baluns, preamplifiers, and connectors. However, volume-type wireless coils are typically operated in transmit/receive mode because detuning such coils is much more challenging due to their complex structure and multiple resonant modes. Meanwhile, adding too many detuning circuits to a wireless coil would decrease the coil's quality factor, impair the signal-to-noise ratio, and increase the cost. In this work, we proposed, constructed, and tested a novel wireless volume coil based on the Litzcage design for 1.5-T head imaging. Being an inductively coupled coil, it has a much simpler structure, resulting in a lighter weight and less bulky design. Despite its simpler structure, it exhibits comparable imaging performance with a commercial receive array, providing an alternative to conventional wired coils with a high cost and complex structure. The unique figure-of-8 conductor pattern within the rungs ensures that the proposed wireless Litzcage can be efficiently detuned with minimal detuning circuits.

Establishment of CRISPR-Cas9 system in Bifidobacteria animalis AR668.

Bifidobacteria are representative intestinal probiotics that have extremely high application value in the food and medical fields. However, the lack of molecular biology tools limits the research on functional genes and mechanisms of bifidobacteria. The application of an accurate and efficient CRISPR system to genome engineering can fill the gap in efficient genetic tools for bifidobacteria. In this study, CRISPR system of B. animalis AR668 was established, which successfully knocked out gene 0348 and gene 0208. The influence of different homology arms and fragments on the knockout effect of the system was explored. In addition, the inducible plasmid curing system of bifidobacteria was innovatively established. This study contributes to the genetic modification and functional mechanism analysis of bifidobacteria.

Enhancement of triterpene production via in situ extractive fermentation of Sanghuangporus vaninii YC-1.

There have been many studies on the activities and polysaccharide production of Sanghuangporus vaninii. However, few studies have looked at triterpene production from S. vaninii using liquid-state fermentation. A method for enhancing the production of triterpenes by in situ extractive fermentation (ISEF) was studied. Eight solvents were investigated as extractants for triterpene production in the ISEF system. The results showed that using vegetable oil as an extractant significantly increased the yield of total triterpenes and biomass of S. vaninii YC-1, reaching 18.98 ± 0.71 and 44.67 ± 2.21 g/L, respectively. In 5 L fermenter experiments, the added vegetable oil improved the dissolved oxygen condition of the fermentation broth and promoted the growth of S. vaninii YC-1. Furthermore, adding vegetable oil increased the expression of fatty acid synthesis-related genes such as FAD2 and SCD, thereby increasing the synthesis of unsaturated fatty acids in the cell membrane of S. vaninii YC-1. Therefore, the cell membrane permeability of S. vaninii YC-1 increased by 19%. Our results indicated that vegetable oil increased the permeability of S. vaninii YC-1 cell membranes to promote the production of total triterpenes. The use of vegetable oil as an extractant was thus effective in increasing the yield of triterpenes in the ISEF system.

Effects of hydrophyte decomposition on the binding mechanism between fluorescent DOM and copper.

Hydrophyte decomposition caused large amounts of dissolved organic matter (DOM) to enter aquatic environment that influence the migration and transformation of heavy metals (HMs). Six hydrophytes with five dry weight gradients (DWG) were used for the decomposition experiments. The results showed that protein-like materials occupy relatively high content in the hydrophyte-derived DOM. The binding properties of DOM-Cu(II) have been explored by using two-dimensional correlation spectroscopy (2D-COS) in conjunction with synchronous fluorescence spectroscopy (SFS) and log-transformed SFS. The weak signals of binding site can be amplified by the log-transformed 2D-COS analysis. Herein, more binding sites can be identified by the log-transformed 2D-COS analysis. The results reveal that tryptophan-like materials show a preferential sequence of binding Cu(II) in the hydrophyte-derived DOM with a relatively low DWG and sediment DOM, and fulvic-like substances indicate a preferential sequence of binding Cu(II) in the hydrophyte-derived DOM with a relatively high DWG. Meanwhile, the results of binding parameters indicate that the log K is the range of 3.61-4.25, 4.33-4.74, 4.59-4.97, 3.91-4.41, and 4.14-4.78 for D1-D5, respectively, suggesting that hydrophyte decomposition can change the binding affinity between DOM components and Cu(II). The complexes of fluorescent components with Cu(II) showed a high log K value at long wavelength (e.g. humic-like substances), and a relatively low fluorescent ligand proportion (f%) at shorter wavelength in the hydrophyte-derived DOM. However, the log K is the range of 3.08-4.31, 4.09-4.45, 3.93-4.35, 4.39-4.75, and 3.95-4.36 for C1-C5, separately. Protein-like substances with Cu(II) showed a relatively high log K value with the exception of C4. The log-transformed 2D-COS can be an analytical tool to understand the binding heterogeneity of DOM with HMs. The study can provide a guide for managing and controlling the effects of hydrophyte decomposition.

The application of two-dimensional correlation spectroscopy for the binding properties of heavy metals onto digestate-derived DOM from anaerobic digestion of chicken manure.

Anaerobic digestate has been widely used for agricultural activities as an organic fertilizer product. Dissolved organic matter (DOM) derived from anaerobic digestate plays a key role in the speciation, bioavailability and ultimate fate of metals that is related to agriculture and food safety as well as the soil environment. Hence, the binding properties of Cu, Pb and Zn with digestate DOM are investigated using two-dimensional correlation spectroscopy (2D-COS) in combination with ultraviolet absorption, synchronous fluorescence spectra (SFS) and Fourier transform infrared (FTIR) spectroscopy. The 2D absorption COS shows that the DOM at 200 nm is most susceptive with the addition of Pb, followed by Zn and Cu. The log-transformed absorption spectra can also obtain more valuable signals than that from conventional absorption spectra. The 2D-SFS-COS indicates that protein-like peak is more sensitive to the variation of the concentration of metal ions, and fulvic-like substances can preferentially interact with the three heavy metals (HMs). The 2D-FTIR-COS reveals that Cu(II) and Zn(II) ions can be bonded preferentially to the N-H of secondary amide (II), and phenolic OH groups shows a favorable binding with Pb(II). Humic-like peaks with Cu(II) and Zn(II) obtains relatively higher log KM values than fulvic- and protein-like substances. However, the proportion of initial fluorescence (f) for DOM-Cu(II) and DOM-Zn(II) decreased with an increase in wavelength. Protein-like materials have more fluorescent substances that can combine with Cu(II) and Zn(II). This study provides a guide for understanding the geochemical behavior of metal ions in agricultural soils when anaerobic digestate is applied as an organic fertilizer product.

Parallel Three-Branch Correlation Filters for Complex Marine Environmental Object Tracking Based on a Confidence Mechanism.

Marine object tracking is critical for search and rescue activities in the complex marine environment. However, the complex marine environment poses a huge challenge to the effect of tracking, such as the variability of light, the impact of sea waves, the occlusion of other ships, etc. Under these complex marine environmental factors, how to design an efficient dynamic visual tracker to make the results accurate, real time and robust is particularly important. The parallel three-branch correlation filters for complex marine environmental object tracking based on a confidence mechanism is proposed by us. The proposed tracker first detects the appearance change and position change of the object by constructing parallel three-branch correlation filters, which enhances the robustness of the correlation filter model. Through the weighted fusion of response maps, the center position of the object is accurately located. Secondly, the Gaussian-triangle joint distribution is used to replace the original Gaussian distribution in the training phase. Finally, a verification mechanism of confidence metric is embedded in the filter update section to analyze the tracking effect of the current frame, and to update the filter sample from verification result. Thus, a more accurate correlation filter is trained to prevent model drift and achieve a good tracking effect. We found that the effect of various interferences on the filter is effectively reduced by comparing with other trackers. The experiments prove that the proposed tracker can play an outstanding role in the complex marine environment.

Isolation of biogenic amine-negative lactic acid bacteria for Chinese rice wine fermentation based on molecular marker reverse screening.

BACKGROUND: Chinese rice wine (CRW; a traditional alcoholic beverage in China with unique flavor and high nutritional value) containing high level of biogenic amines (BAs) may be deleterious to human health. The processes of rice soaking, primary fermentation and secondary fermentation were found to be the major factors for accumulation of BAs during industrial CRW production. RESULTS: To reduce the risk of the formation of BAs in CRW production, Enterococcus durans AR315, a BA-negative lactic acid bacterium, was isolated from CRW samples by PCR-based molecular marker reverse screening in this work. With addition of AR315 during steeping rice phase, the level of total BAs was significantly decreased by 45.1% in comparison with the control. Moreover, the final BA concentration with the addition of AR315 was 27.6% lower than that of the control during fermentation phase. CONCLUSIONS: To our knowledge, this is the first report of decreased accumulation of BAs in CRW production using a BA-negative lactic acid bacterium. Hence, using a BA-negative lactic acid bacterium as a starter culture could be an efficient strategy for significantly reducing the formation of BAs, which has the potential for industrial application in CRW production. © 2020 Society of Chemical Industry.

Membrane Fluidity of Saccharomyces cerevisiae from Huangjiu (Chinese Rice Wine) Is Variably Regulated by OLE1 To Offset the Disruptive Effect of Ethanol.

An evolution and resequencing strategy was used to research the genetic basis of Saccharomyces cerevisiae BR20 (with 18 vol% ethanol tolerance) and the evolved strain F23 (with 25 vol% ethanol tolerance). Whole-genome sequencing and RNA sequencing (RNA-seq) indicated that the enhanced ethanol tolerance under 10 vol% ethanol could be attributed to amino acid metabolism, whereas 18 vol% ethanol tolerance was due to fatty acid metabolism. Ultrastructural analysis indicated that F23 exhibited better membrane integrity than did BR20 under ethanol stress. At low concentrations (<5 vol%), the partition of ethanol into the membrane increased the membrane fluidity, which had little effect on cell growth. However, the toxic effects of medium and high ethanol concentrations (5 to 20 vol%) tended to decrease the membrane fluidity. Under high ethanol stress (>10 vol%), the highly tolerant strain was able to maintain a relatively constant fluidity by increasing the content of unsaturated fatty acid (UFA), whereas less-tolerant strains show a continuous decrease in fluidity and UFA content. OLE1, which was identified as the only gene with a differential single-nucleotide polymorphism (SNP) mutation site related to fatty acid metabolism, was significantly changed in response to ethanol. The role of OLE1 in membrane fluidity was positively validated in its overexpressed transformants. Therefore, OLE1 lowered the rate of decline in membrane fluidity and thus enabled the yeast to better fight the deleterious effects of ethanol.IMPORTANCE Yeasts with superior ethanol tolerance are desirable for winemakers and wine industries. In our previous work, strain F23 was evolved with superior ethanol tolerance and fermentation activity to improve the flavor profiles of Chinese rice wine. Therefore, exploring the genomic variations and ethanol tolerance mechanism of strain F23 could contribute to an understanding of its effect on the flavor characteristics in the resulting Chinese rice wine. The cellular membrane plays a vital role in the ethanol tolerance of yeasts; however, how the membrane is regulated to fight the toxic effect of ethanol remains to be elucidated. This study suggests that the membrane fluidity is variably regulated by OLE1 to offset the disruptive effect of ethanol. Current work will help develop more ethanol-tolerant yeast strains for wine industries and contribute to a deep understanding of its high flavor-producing ability.

Parallel Correlation Filters for Real-Time Visual Tracking.

Correlation filter-based methods have recently performed remarkably well in terms of accuracy and speed in the visual object tracking research field. However, most existing correlation filter-based methods are not robust to significant appearance changes in the target, especially when the target undergoes deformation, illumination variation, and rotation. In this paper, a novel parallel correlation filters (PCF) framework is proposed for real-time visual object tracking. Firstly, the proposed method constructs two parallel correlation filters, one for tracking the appearance changes in the target, and the other for tracking the translation of the target. Secondly, through weighted merging the response maps of these two parallel correlation filters, the proposed method accurately locates the center position of the target. Finally, in the training stage, a new reasonable distribution of the correlation output is proposed to replace the original Gaussian distribution to train more accurate correlation filters, which can prevent the model from drifting to achieve excellent tracking performance. The extensive qualitative and quantitative experiments on the common object tracking benchmarks OTB-2013 and OTB-2015 have demonstrated that the proposed PCF tracker outperforms most of the state-of-the-art trackers and achieves a high real-time tracking performance.

Motion-Aware Correlation Filters for Online Visual Tracking.

The discriminative correlation filters-based methods struggle deal with the problem of fast motion and heavy occlusion, the problem can severely degrade the performance of trackers, ultimately leading to tracking failures. In this paper, a novel Motion-Aware Correlation Filters (MACF) framework is proposed for online visual object tracking, where a motion-aware strategy based on joint instantaneous motion estimation Kalman filters is integrated into the Discriminative Correlation Filters (DCFs). The proposed motion-aware strategy is used to predict the possible region and scale of the target in the current frame by utilizing the previous estimated 3D motion information. Obviously, this strategy can prevent model drift caused by fast motion. On the base of the predicted region and scale, the MACF detects the position and scale of the target by using the DCFs-based method in the current frame. Furthermore, an adaptive model updating strategy is proposed to address the problem of corrupted models caused by occlusions, where the learning rate is determined by the confidence of the response map. The extensive experiments on popular Object Tracking Benchmark OTB-100, OTB-50 and unmanned aerial vehicles (UAV) video have demonstrated that the proposed MACF tracker performs better than most of the state-of-the-art trackers and achieves a high real-time performance. In addition, the proposed approach can be integrated easily and flexibly into other visual tracking algorithms.

MiR-335 functions as a tumor suppressor in pancreatic cancer by targeting OCT4.

Octamer-binding transcription factor 4 (OCT4) was closely related to pancreatic cancer progression, but its regulation in pancreatic cancer by microRNA (miRNA) is not fully clear. OCT4-positive and OCT4-negative pancreatic cells were isolated by flow cytometry, and it was found that OCT4-positive cells are enriched in transplanted pancreatic cancer cells compared with the primary ones and showed increasing proliferation and sphere formation. The data of miRNA array assay showed that miR-335 in OCT4-positive pancreatic cancer cells was lower than that in the negative ones. The results were confirmed in pancreatic cancer tissue and cell lines. Through expression analysis, it was found that miR-335 was underexpressed in OCT4(+) pancreatic cancer cells purified from primary tumors. Enforced expression of miR-335 in OCT4(+) pancreatic cancer cells inhibited clonogenic expansion and tumor development. miR-335 re-expression in OCT4(+) pancreatic cancer cells was blocked. Systemically delivered miR-335 inhibited pancreatic cancer metastasis and extended animal survival. Of significance, OCT4 was identified and validated as a direct and functional target of miR-335. Taken together, our results provide evidence that miR-335 might inhibit progression and stem cell properties of pancreatic cancer targeting OCT4.

A randomized clinical trial of nerve block to manage end-stage pancreatic cancerous pain.

Pain control is the most difficult puzzle in patients with terminal pancreatic cancerous pain. Many methods in clinical practice fail in 20 ~ 50% of patients. The present study aims to explore the effect of nerve block on patients with end-stage pancreatic cancerous pain. In this study, 100 subjects with end-stage pancreatic cancerous pain were enrolled and randomly assigned into two groups: 68 in nerve block group (N) and 32 in sham group (S). One group was treated with nerve block and the other group with sham procedure as controls. The pain score (by visual analog scale (VAS)), pain duration, reduction of other analgesic medications, and quality of life (with questionnaire QLQ) were evaluated before and 3 months after interventions. Comparisons were performed between before and after intervention in nerve block group and sham group. The results indicated that compared with sham group, the subjects in nerve block group had significant reduction with pain score, pain duration, and other analgesic medications, as well as improvement of quality of life (P < 0.05, respectively). In conclusion, nerve block is an effective method for treating patients with end-stage pancreatic cancerous pain.

Who holds the social power? The development of children's social power perceptions in China.

This study examined the development of social power perceptions among Chinese children aged 3-5 years (N = 105). After watching videos about various social power cues, such as resource possession, resource control, goal achievement, permission, giving orders, setting norms and popularity, the children were asked to identify the powerful agents (whom do you believe is the more powerful person?) in the videos and provide explanations (why do you think he (she) is a powerful person?). Three-year-olds can recognize powerful agents who can grant 'permission' to other agents. By the age of 4, children begin to associate 'popularity', 'resource possession' and 'goal achievement' with social power. Five-year olds demonstrated the ability to recognize agents who control resources as being more powerful. Analysis of the reasons the children provided for their judgements revealed that for almost every cue (except giving orders), more than 14% of the responses highlighted 'possession of material resources' as an indicator of power. For children aged 3-5 years, 'resource possession' cues may be their preferred basis for inferring and explaining power differences. These results would facilitate researchers to further unravel the mechanisms underlying the development of children's social power perceptions.

A Cross Talking between the Gut Microbiota and Metabolites of Participants in a Confined Environment.

Certain workplaces, like deep-sea voyages, subject workers to chronic psychological stress and circadian rhythm disorders due to confined environments and frequent shifts. In this study, participants lived in a strictly controlled confined environment, and we analyzed the effects of a confined environment on gut microbiota and metabolites. The results showed that living in confined environments can significantly alter both the gut microbiota and the gut metabolome, particularly affecting lipid metabolism pathways like glycerophospholipid metabolism. There was a significant reduction in the abundance of Faecalibacterium and Bacteroides, while Blautia, Bifidobacterium, and Collinsella showed significant increases. An association analysis revealed a strong correlation between changes in the gut microbiota and the metabolome. Four upregulated lipid metabolites may serve as biomarkers for damage induced by confined environments, and certain gut microbiota alterations, such as those involving Faecalibacterium and Bacteroides, could be potential psychobiotics or therapeutic targets for enhancing mental health in a confined environment.

[Analysis of SCA3/MJD3 gene mutation and genetic polymorphism in a guangxi family with spinocerebellar ataxia 3].

Autosomal dominant cerebellar ataxias (ADCAs) comprise a group of genetically heterogeneous neurodegenerative disorders among which spinocerebellar ataxia type 3 (SCA3) represents the most common form of SCAs worldwide. The fragments of SCA3/MJD gene,which is the member of family GXPL1,were amplified by polymerase chain reaction (PCR). The PCR products of SCA3/MJD gene were detected with capillary electrophoresis (CE) and sequencing to evaluate the size of CAG repeats, feature in the transmission and the mutation in the family with SCA3 in Guangxi province. The results showed that the exon 10 of the SCA3/MJD gene contains 64-71 CAG repeats in all of the affected individuals and three asymptomatic carriers of the family. The number of the CAG repeats during transmission in the normal individuals carrying CGG allele remains consistent, suggesting that CGG allele could have no effect on intergenerational stability of CAG repeats in normal individuals. In addition, two novel point mutations were identified: IVS9-113 T > C in the intronic region and a missense mutation 220 G > A (Glu > Gly) in the encoding region. These two novel point mutations have not been reported and the effect of the mutations on the phenotype of SCA3 is not clear.

Joint Correlation and Attention Based Feature Fusion Network for Accurate Visual Tracking.

Correlation operation and attention mechanism are two popular feature fusion approaches which play an important role in visual object tracking. However, the correlation-based tracking networks are sensitive to location information but loss some context semantics, while the attention-based tracking networks can make full use of rich semantic information but ignore the position distribution of the tracked object. Therefore, in this paper, we propose a novel tracking framework based on joint correlation and attention networks, termed as JCAT, which can effectively combine the advantages of these two complementary feature fusion approaches. Concretely, the proposed JCAT approach adopts parallel correlation and attention branches to generate position and semantic features. Then the fusion features are obtained by directly adding the location feature and semantic feature. Finally, the fused features are fed into the segmentation network to generate the pixel-wise state estimation of the object. Furthermore, we develop a segmentation memory bank and an online sample filtering mechanism for robust segmentation and tracking. The extensive experimental results on eight challenging visual tracking benchmarks show that the proposed JCAT tracker achieves very promising tracking performance and sets a new state-of-the-art on the VOT2018 benchmark.

bsh1 Gene of Lactobacillus plantarum AR113 Plays an Important Role in Ameliorating Western Diet-Aggravated Colitis.

Western diet is thought to increase susceptibility to inflammatory bowel disease (IBD), and probiotics are a potential therapeutic agent for IBD. This study revealed the effects of Lactobacillus plantarum AR113 and L. plantarum AR113Δbsh1 on a dextran sulfate sodium (DSS)-induced colitis mouse model under the Western diet (WD). After four weeks of WD and low-sugar and low-fat diet (LD) intervention, induction with 3% DSS, and intragastric administration of probiotics, we found that L. plantarum AR113 could regulate blood glucose and lipid levels and have a certain protective effect on hepatocytes. Our results suggested that the L. plantarum AR113 alleviated DSS-induced colitis under the Western diet by improving dyslipidemia, repairing intestinal barrier dysfunction, and inhibiting the TLR4/Myd88/TRAF-6/NF-κB inflammatory pathway. However, these changes were not demonstrated in the L. plantarum AR113Δbsh1, and therefore, we reasoned that the presence of bsh1 may play a crucial role in the L. plantarum AR113 exerting its anti-inflammatory function. The relationship between bile salt hydrolase (BSH) and colitis was worthy of further exploration.

Flavor compounds with high odor activity values (OAV > 1) dominate the aroma of aged Chinese rice wine (Huangjiu) by molecular association.

Aging is an essential operation to perfect the flavor quality of Hungjiu. In this study, formation mechanism of flavor compounds responsible for the characteristic flavor of aged Huangjiu was investigated. The contents of umami and bitter free amino acids (FAA) increased with the storage period prolonged, while that of sweet FAA showed downward trend. Gas chromatograph-mass spectrometry and principal component analysis indicated that the volatile flavor compounds with OAV exceed 1, especially middle-chain fatty-acid-ethyl-esters and aromatic compounds, dominated the characteristic flavor of aged Huangjiu. Low field-NMR was firstly applied to characterize the molecular association between water and dissolved flavor compounds in aged Huangjiu. The results showed that basic amino acids contributed greatly to the flavor formation of aged Huangjiu via molecular association. In addition, the molecular association significantly promoted the accumulation of flavor compounds with OAV > 1, especially ethyl esters.

Probiotic yeast BR14 ameliorates DSS-induced colitis by restoring the gut barrier and adjusting the intestinal microbiota.

The probiotic Saccharomyces boulardii has been widely used in colitis treatment; however, the beneficial effects of other yeast species are rarely studied. Saccharomyces cerevisiae with great stress tolerance and potential in colitis treatment was investigated in this study. Among 16 yeast strains, BR14, BR54, and BR174 strains showed good stress-resistant capacity, anti-inflammatory activity, and little toxicity to macrophages. As for the colitis mice, BR14 inhibited weight loss the most, as well as the disease activity index and colon shortening. After treatment with BR14, the expression levels of genes related to histological damage were all upregulated. BR14 significantly attenuated the expression levels of TNF-α and IL-6, while the expression of IL-10 was upregulated. Additionally, BR14 rebalanced the intestinal microbial composition of colitis mice by increasing the abundance of Muribaculaceae, Lactobacillus and Rikenellaceae and decreasing the abundance of Turicibacter, Escherichia-Shigella, Desulfovibrio, and Lachnospiraceae. In summary, BR14 exhibited great potential in alleviating colitis through restoring the gut barrier and adjusting the intestinal microbiota.