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J Virol ; 81(6): 2957-69, 2007 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-17215283

RESUMEN

Rhesus rhadinovirus (RRV) is closely related to Kaposi's sarcoma-associated herpesvirus (KSHV)/human herpesvirus 8 (HHV-8) and causes KSHV-like diseases in immunocompromised rhesus macaques (RM) that resemble KSHV-associated diseases including multicentric Castleman's disease and non-Hodgkin's lymphoma. RRV retains a majority of open reading frames (ORFs) postulated to be involved in the pathogenesis of KSHV and is the closest available animal model to KSHV infection in humans. Here we describe the generation of a recombinant clone of RRV strain 17577 (RRV(17577)) utilizing bacterial artificial chromosome (BAC) technology. Characterization of the RRV BAC demonstrated that it is a pathogenic molecular clone of RRV(17577), producing virus that behaves like wild-type RRV both in vitro and in vivo. Specifically, BAC-derived RRV displays wild-type growth properties in vitro and readily infects simian immunodeficiency virus-infected RM, inducing B cell hyperplasia, persistent lymphadenopathy, and persistent infection in these animals. This RRV BAC will allow for rapid genetic manipulation of the RRV genome, facilitating the creation of recombinant versions of RRV that harbor specific alterations and/or deletions of viral ORFs. This system will provide insights into the roles of specific RRV genes in various aspects of the viral life cycle and the RRV-associated pathogenesis in vivo in an RM model of infection. Furthermore, the generation of chimeric versions of RRV containing KSHV genes will allow analysis of the function and contributions of KSHV genes to viral pathogenesis by using a relevant primate model system.


Asunto(s)
Cromosomas Artificiales Bacterianos , Herpesvirus Humano 8/genética , Macaca mulatta/virología , Rhadinovirus/genética , Sarcoma de Kaposi/etiología , Animales , Células Cultivadas , ADN Viral/análisis , Fibroblastos/virología , Herpesvirus Humano 8/aislamiento & purificación , Humanos , Hibridación de Ácido Nucleico , Sistemas de Lectura Abierta , Reacción en Cadena de la Polimerasa , Rhadinovirus/química , Sarcoma de Kaposi/virología , Análisis de Secuencia de ADN
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