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1.
Plant Cell ; 33(10): 3293-3308, 2021 10 11.
Artículo en Inglés | MEDLINE | ID: mdl-34338777

RESUMEN

The degree of stigma exsertion has a major influence on self-pollination efficiency in tomato, and its improvement is essential for raising productivity and for fixing advantageous traits in cultivated tomato. To study the evolution of stigma exsertion degree in tomato, we searched for genes associated with this trait and other aspects of flower morphology, including the lengths of anthers, styles, and ovaries. We performed a genome-wide association on 277 tomato accessions and discovered a novel stigma exsertion gene (SE3.1). We reannotated the structure of the gene, which encodes a C2H2-type zinc finger transcription factor. A mutation of the lead single nucleotide polymorphism creates a premature termination codon in SE3.1 and an inserted stigma in cultivated tomatoes. SE3.1 is essential for the conversion of flush stigmas to inserted stigmas. This conversion has a major impact on the rate of self-fertilization. Intriguingly, we found that both SE3.1 and Style2.1 contribute to the transition from stigma exsertion to insertion during the domestication and improvement of tomato. Style2.1 controls the first step of exserted stigmas to flush stigmas, and SE3.1 controls the second step of flush stigmas to inserted stigmas. We provide molecular details for the two-step process that controls the transition from stigma exsertion to insertion, which is of great agronomic importance in tomato.


Asunto(s)
Estudio de Asociación del Genoma Completo , Proteínas de Plantas/genética , Polinización/genética , Solanum lycopersicum/fisiología , Factores de Transcripción/genética , Solanum lycopersicum/genética , Mutación , Proteínas de Plantas/metabolismo , Factores de Transcripción/metabolismo
2.
Entropy (Basel) ; 24(7)2022 Jul 18.
Artículo en Inglés | MEDLINE | ID: mdl-35885219

RESUMEN

Under the framework of computational integral imaging, a multi-image encryption scheme based on the DNA-chaos algorithm is proposed. In this scheme, multiple images are merged to one image by a computational integral imaging algorithm, which significantly improves the efficiency of image encryption. Meanwhile, the computational integral imaging algorithm can merge images at different depth distances, thereby the different depth distances of multiple images can also be used as keys to increase the security of the encryption method. In addition, the high randomness of the chaos algorithm is combined to address the outline effect caused by the DNA encryption algorithm. We have experimentally verified the proposed multi-image encryption scheme. The entropy value of the encrypted image is 7.6227, whereas the entropy value of the merge image with two input images is 3.2886, which greatly reduces the relevance of the image. The simulation results also confirm that the proposed encryption scheme has high key security and can protect against various attacks.

3.
Entropy (Basel) ; 24(10)2022 Oct 03.
Artículo en Inglés | MEDLINE | ID: mdl-37420434

RESUMEN

In this paper, we present the multi-stage attentive network (MSAN), an efficient and good generalization performance convolutional neural network (CNN) architecture for motion deblurring. We build a multi-stage encoder-decoder network with self-attention and use the binary cross-entropy loss to train our model. In MSAN, there are two core designs. First, we introduce a new attention-based end-to-end method on top of multi-stage networks, which applies group convolution to the self-attention module, effectively reducing the computing cost and improving the model's adaptability to different blurred images. Secondly, we propose using binary cross-entropy loss instead of pixel loss to optimize our model to minimize the over-smoothing impact of pixel loss while maintaining a good deblurring effect. We conduct extensive experiments on several deblurring datasets to evaluate the performance of our solution for deblurring. Our MSAN achieves superior performance while also generalizing and compares well with state-of-the-art methods.

4.
Inorg Chem ; 60(12): 8507-8518, 2021 Jun 21.
Artículo en Inglés | MEDLINE | ID: mdl-34060816

RESUMEN

The sequence of transitions between different phases of BiNbO4 has been thoroughly investigated and clarified using thermal analysis, high-resolution neutron diffraction, and Raman spectroscopy. The theoretical optical phonon modes of the α-phase have been calculated. Based on thermoanalytical data supported by density functional theory (DFT) calculations, the ß-phase is proposed to be metastable, while the α- and γ-phases are stable below and above 1040 °C, respectively. Accurate positional parameters for oxygen positions in the three main polymorphs (α, ß, and γ) are presented and the structural relationships between these polymorphs are discussed. Even though no significant changes, only relaxation phenomena, are observed in the dielectric behavior of α-BiNbO4 below 1000 °C, evidence of two further subtle transitions at ∼350 and 600 °C is presented through careful analysis of structural parameters from variable temperature neutron diffraction measurements. Such phase variations are also evident in the phonon modes in Raman spectra and supported by changes in the thermoanalytical data. These subtle transitions may correspond to the previously proposed antiferroelectric to ferroelectric and ferroelectric to paraelectric phase transitions, respectively.

5.
Opt Express ; 27(23): 34284-34297, 2019 Nov 11.
Artículo en Inglés | MEDLINE | ID: mdl-31878479

RESUMEN

We demonstrated a diversity-reception lens-free underwater wireless optical communication system employing a 2×2 solar panel array as detectors. The respective relationships between solar panel sizes and photocurrents, output voltages, system bandwidths were studied theoretically and experimentally. The signals output from the array were combined via maximum ratio combining in order to improve the signal quality. A 450-nm blue laser was used in the transmitter and a light spot with a size of 20mm×35mm was formed on the receiving plane after a 7-m transmission in tap water. With the solar panel array, a data rate of 84 Mbps was achieved with a bit error rate of 2.17 × 10-3 using 16-QAM OFDM signal. Meanwhile, a single solar panel with the same size as the array only achieved 60 Mbps. Solar panel array can bring about 40% data rate improvement. With the same detection area, the horizontal detection range of detectors increased from 37 mm for single solar panel to 55 mm for solar panel array thanks to the diversity reception. The results show that solar panel array using maximum ratio combining can enhance the transmission data rate as well as the detection range. The impacts of air bubbles, water fluctuation and microscopic particulates suspension on the proposed solar panel array-based system were also investigated. The results show that the received power sensitivity of solar panel array is 5.22 dB higher than single solar panel with the same detection area with a data rate of 60 Mbps and a BER of 10-3.

6.
Entropy (Basel) ; 21(5)2019 May 02.
Artículo en Inglés | MEDLINE | ID: mdl-33267175

RESUMEN

A novel adaptive secure holographic image watermarking method in the sharp frequency localized contourlet transform (SFLCT) domain is presented. Based upon the sine logistic modulation map and the logistic map, we develop an encrypted binary computer-generated hologram technique to fabricate a hologram of a watermark first. Owing to the enormous key space of the encrypted hologram, the security of the image watermarking system is increased. Then the hologram watermark is embedded into the SFLCT coefficients with Schur decomposition. To obtain better imperceptibility and robustness, the entropy and the edge entropy are utilized to select the suitable watermark embedding positions adaptively. Compared with other watermarking schemes, the suggested method provides a better performance with respect to both imperceptibility and robustness. Experiments show that our watermarking scheme for images is not only is secure and invisible, but also has a stronger robustness against different kinds of attack.

7.
Opt Express ; 25(17): 20829-20834, 2017 Aug 21.
Artículo en Inglés | MEDLINE | ID: mdl-29041760

RESUMEN

The availability of the underwater wireless optical communication (UWOC) based on red (R), green (G) and blue (B) lights makes the realization of the RGB wavelength division multiplexing (WDM) UWOC system possible. By properly mixing RGB lights to form white light, the WDM UWOC system has prominent potentiality for simultaneous underwater illumination and high-speed communication. In this work, for the first time, we experimentally demonstrate a 9.51-Gb/s WDM UWOC system using a red-emitting laser diode (LD), a single-mode pigtailed green-emitting LD and a multi-mode pigtailed blue-emitting LD. By employing 32-quadrature amplitude modulation (QAM) orthogonal frequency division multiplexing (OFDM) modulation in the demonstration, the red-light, the green-light and the blue-light LDs successfully transmit signals with the data rates of 4.17 Gb/s, 4.17 Gb/s and 1.17 Gb/s, respectively, over a 10-m underwater channel. The corresponding bit error rates (BERs) are 2.2 × 10-3, 2.0 × 10-3 and 2.3 × 10-3, respectively, which are below the forward error correction (FEC) threshold of 3.8 × 10-3.

8.
Biochem Biophys Res Commun ; 468(4): 653-9, 2015 Dec 25.
Artículo en Inglés | MEDLINE | ID: mdl-26612258

RESUMEN

The trihelix family, classified as GT factors due to their binding specificity for GT elements, constitutes a plant-specific transcription factor family with a conserved trihelix DNA binding domain. In the present study, the comprehensive analysis of 36 putative GT factors was performed in tomato. SlGT members can be classified into six subgroups (GT-1, GT-2, SH4, SIP1, GT-γ and GT-δ). Expression analysis of SlGT gene transcripts showed the distinct expression patterns of SlGT genes in various tomato organs. All the SlGT genes were regulated in response to various abiotic stresses and hormone treatments by the quantitative real-time PCR (qRT-PCR) analysis. Several SlGT genes, including SlGT-27 and SlGT-34, were highly regulated by multiple abiotic stresses and phytohormone treatments. Taken together, our results presented here would be providing a useful platform for molecular clone and functional identification of SlGT genes in tomato.


Asunto(s)
Regulación de la Expresión Génica de las Plantas/genética , Genoma de Planta/genética , Proteínas de Plantas/química , Proteínas de Plantas/genética , Solanum lycopersicum/genética , Factores de Transcripción/química , Perfilación de la Expresión Génica/métodos , Estudio de Asociación del Genoma Completo/métodos , Solanum lycopersicum/metabolismo , Distribución Tisular , Factores de Transcripción/genética
9.
J Exp Bot ; 66(5): 1413-26, 2015 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-25680796

RESUMEN

Plants integrate responses to independent hormonal and environmental signals to survive adversity. In particular, the phytohormone gibberellin (GA) regulates a variety of developmental processes and stress responses. In this study, the Jumonji-C (JmjC) domain-containing gene JMJ524 was characterized in tomato. JMJ524 responded to circadian rhythms and was upregulated by GA treatment. Knockdown of JMJ524 by RNAi caused a GA-insensitive dwarf phenotype with shrunken leaves and shortened internodes. However, in these transgenic plants, higher levels of endogenous GAs were detected. A genome-wide gene expression analysis by RNA-seq indicated that the expression levels of two DELLA-like genes, SlGLD1 ('GRAS protein Lacking the DELLA domain') and SlGLD2, were increased in JMJ524-RNAi transgenic plants. Nevertheless, only the overexpression of SlGLD1 in tomato resulted in a GA-insensitive dwarf phenotype, suggesting that SlGLD1 acts as a repressor of GA signalling. This study proposes that JMJ524 is required for stem elongation by altering GA responses, at least partially by regulating SlGLD1.


Asunto(s)
Giberelinas/metabolismo , Reguladores del Crecimiento de las Plantas/metabolismo , Proteínas de Plantas/genética , Solanum lycopersicum/genética , Secuencia de Aminoácidos , Técnicas de Silenciamiento del Gen , Solanum lycopersicum/química , Solanum lycopersicum/clasificación , Solanum lycopersicum/metabolismo , Datos de Secuencia Molecular , Filogenia , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Estructura Terciaria de Proteína , Alineación de Secuencia
10.
ACS Omega ; 9(2): 2234-2249, 2024 Jan 16.
Artículo en Inglés | MEDLINE | ID: mdl-38250425

RESUMEN

Developing wearable supercapacitors (SCs) with high stretchability, arbitrary deformability, and antifreezing ability is still a challenge. In the present work, an ultrahigh-ionic-conductivity, antifreezing poly(amidoxime)-graft-polyzwitterion (PAO-g-PSBMA) hydrogel electrolyte is fabricated by grafting PSBMA in PAO. Owing to the abundant hydrophilic and high ionic adsorption capacity of amidoxime groups in PAO and zwitterion groups in PSBMA, the as-prepared PAO-g-PSBMA hydrogel can facilitate the dissociation of lithium salt and exhibit an ultrahigh ionic conductivity of 29.8 S m-1 at 25 °C and 3.4 S m-1 even at -30 °C. Employing mATi3C2Tx and mSTi3C2Tx, which contain small amounts of PAO-AGE and PAO-g-PSBMA dispersions, respectively, coated onto both sides of the PAO-g-PSBMA hydrogel, we followed a thermal treatment to facilely form integrated stretchable flexible SCs. The as-prepared SCs show an outstanding recoverable tensile stain of 80% and an excellent electrochemical stability under many types and times of arbitrary deformation. More importantly, as-prepared mATi3C2Tx- and mSTi3C2Tx-based SCs present fantastic antifreezing ability and excellent stability with 74.6 and 78.3% retention of the initial capacitance, respectively, even after 1000 times of stretching to 60% at -30 °C. This work offers a new strategy of using PAO-grafted polyzwitterion for obtaining an antifreezing stretchable SC, which shows a high potential for application in next-generation integrated stretchable devices in various fields.

11.
Front Plant Sci ; 14: 1140851, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-37056500

RESUMEN

The purple color of unripe pepper fruit is attributed to the accumulation of anthocyanins. Only a few genes controlling the biosynthesis and regulation of anthocyanins have been cloned in Capsicum. In this study, we performed a bulked segregant analysis of the purple striped trait using an F2 population derived from a cross between the immature purple striped fruit line Chen12-4-1-1-1-1 and the normal green fruit line Zhongxian101-M-F9. We mapped the CaPs locus to an 841.39 kb region between markers M-CA690-Xba and MCA710-03 on chromosome 10. CA10g11690 encodes an R2R3-MYB transcription factor that is involved in the biosynthesis of anthocyanins as the best candidate gene. Overexpression and silencing in transformed tobacco (Nicotiana tabacum) lines indicated that CA10g11690 is involved in the formation of purple stripes in the exocarp. A comparison of parental sequences identified an insertion fragment of 1,926 bp in the second intron region of Chen12-4, and eight SNPs were detected between the two parents. Additionally, there were 49 single nucleotide polymorphic variations, two sequence deletions, and four sequence insertions in the promoter region. We found that CA10g11690 undergoes alternative splicing and generates different transcripts. Thus, the functional transcript of CA10g11690 appeared to be primarily involved in the development of purple phenotype in the exocarp. Our data provide new insight into the mechanism of anthocyanin biosynthesis and a theoretical basis for the future breeding of purple striped pepper varieties.

12.
ACS Appl Mater Interfaces ; 14(15): 17739-17753, 2022 Apr 20.
Artículo en Inglés | MEDLINE | ID: mdl-35389612

RESUMEN

The development of a novel preparation strategy for 3D porous network structures with an aligned channel or wall is always in challenge. Herein, a 3D porous network composed of an aligned graphene-based wall is fabricated by a confined self-assembly strategy in which holey reduced graphene oxide (HrGO)/lignin sulfonate (Lig) composites are orientedly anchored on the framework of the Lig/single-wall carbon nanotube (Lig/SWCNT) hydrogel by vacuum-assisted filtration accompanied with confined self-assembly and followed with hydrothermal treatment. After freeze drying, the obtained ultralight Lig/SWCNT/HrGOal aerogel exhibits excellent shape memory properties and can roll back to the original shape even if suffering from a high compressive strain of 86.2%. Furthermore, the as-prepared aerogel used as a water-driven artificial muscle shows powerful driving force and can lift ultrahigh weight cargo that is 1030.6 times its own weight. When the prepared Lig/SWCNT/HrGOal aerogel is used as a pressure sensor, it also exhibits high sensitivity (2.28 kPa-1) and a wide detection region of 0.27-14.1 kPa. Additionally, the symmetric flexible supercapacitor assembled with as-prepared aerogel films shows superior stored energy performance that can tolerate 5000 cycles of bending. The present work not only fabricates a high-performance multifunctional material but also develops a new strategy for the preparation a wood-like 3D porous aligned wall network structure.

13.
ACS Appl Mater Interfaces ; 14(15): 17858-17868, 2022 Apr 20.
Artículo en Inglés | MEDLINE | ID: mdl-35390255

RESUMEN

The preparation of graphene-based aerogels with excellent mechanical strength, elasticity, and compressibility is still a challenge. Herein, we demonstrate a robust, elastic, and lightweight graphene/aramid nanofiber/polyaniline nanotube (rGO/ANF/PANIT) aerogel that is prepared by mixing graphene oxide (GO), ANF, and PANIT dispersions, followed by thermal treatment at 90 °C, freeze-drying, and a low-temperature annealing process. The PANIT bonds the graphene sheets tightly, benefitting the formation of composite gels. The ANF tightly interconnects the graphene sheets and further reinforces the composite network framework significantly, hence endowing rGO/ANF/PANIT composite aerogels with robust mechanical property. The prepared aerogels present a low density of ∼12 mg cm-3, high conductivity, good resilience, and high compressibility. The rGO/ANF/PANIT aerogels as pressure sensors exhibit a high sensitivity of 1.73 kPa-1, low detection limit (40 Pa), wide detection range, and excellent compressive cycle stability, highlighting the promising applications in pressure-sensitive electrical devices, including medical health detection, wearable electronics, and intelligent packaging fields.

14.
Nanoscale ; 13(39): 16734-16747, 2021 Oct 14.
Artículo en Inglés | MEDLINE | ID: mdl-34596183

RESUMEN

To endow high mechanical strength and thermal stability aramid nanofibers (ANF) with novel functionality will lead to great applications. Herein, a strategy to generate covalent bonds among components towards obtaining uniform ANF/reduced holey graphene oxide (ANF/rHGO) and ANF/rHGO/polyaniline (ANF/rHGO/PANI) hydrogels with high mechanical properties is proposed through solvent exchange gelation and subsequent hydrothermal treatment. The as-prepared ANF/rHGO and ANF/rHGO/PANI hydrogels demonstrate excellent recoverability at high compressive strength of 20.2 and 13.8 kPa with a strain of 34.4% and 30.6%, respectively, compared to a recoverability of 92.5% at a strain of ∼20% for ANF hydrogels. Moreover, ANF/rHGO and ANF/rHGO/PANI aerogels possess fast and high oil absorption capacity of 38.9-64.1 g g-1 and 24.5-44.0 g g-1, respectively. ANF/rHGO and ANF/rHGO/PANI films obtained after vacuum-drying exhibit a high tensile strength of 121.4 and 95.5 MPa, respectively. Additionally, ANF/rHGO/PANI thin films present good selective absorption of visible light by controlling the doping level of PANI. ANF/rHGO/PANI aerogel films prepared by freeze-drying are assembled into flexible solid-state symmetric supercapacitors and deliver a favorable specific capacitance of 200 F g-1, a desirable capacitance retention of 98.9% after 2500 mechanical bending cycles and an approximately 100% capacitance retention even after keeping tensile force for 15 h. The as-prepared hydrogels, aerogels and derived films with such excellent performances are promising for applications in oil pollution removal, optical filters and flexible load-bearing energy storage devices.

15.
Nanoscale ; 13(5): 3079-3091, 2021 Feb 11.
Artículo en Inglés | MEDLINE | ID: mdl-33522537

RESUMEN

Two dimensional MXenes with fascinating characteristics of high electrical conductivity, high density and electroactivity show promising applications in various fields. However, the direct applications of MXenes have been limited due to their inferior mechanical properties and easy restacking. Herein, a kind of nacre-like composite film constructed with Ti3C2Tx, cellulose nanofiber (HCNF) and sodium lignosulfonate (Lig) obtained through the hydrothermal process, named Ti3C2Tx/HCNF@Lig, has been successfully synthesized. The hydrothermal cellulose nanofiber (HCNF) film shows an enhanced mechanical strength (114 MPa) compared to that of the CNF film (95 MPa). Wood-inspired HCNF@Lig composite films present an enhanced mechanical tensile strength of up to 133 MPa. Nacre-like deformable Ti3C2Tx/HCNF@Lig(3@1) composite films exhibit high conductivity (up to 1.75 × 105 S m-1) and mechanical properties (up to 258 MPa). The electrodes of Ti3C2Tx/HCNF@Lig(3@1)97/3 composite film assembled flexible solid-state supercapacitors possess an excellent volumetric specific capacitance of 748.96 F cm-3. The corresponding deformable supercapacitors show an excellent energy density of 16.2 W h L-1 and outstanding electrochemical cycling stability. The as-prepared nacre-like Ti3C2Tx/HCNF@Lig composite films with high mechanical properties and electrochemical performance are expected to be practically applied in flexible/wearable energy storage devices.

16.
J Appl Genet ; 62(2): 265-280, 2021 May.
Artículo en Inglés | MEDLINE | ID: mdl-33624251

RESUMEN

Pepper (Capsicum annuum L.) is an economically important vegetable crop whose production and quality are severely reduced under adverse environmental stress conditions. The GATA transcription factors belonging to type IV zinc-finger proteins, play a significant role in regulating light morphogenesis, nitrate assimilation, and organ development in plants. However, the functional characteristics of GATA gene family during development and in response to environmental stresses have not yet been investigated in pepper. In this study, a total of 28 pepper GATA (CaGATA) genes were identified. To gain an overview of the CaGATAs, we analyzed their chromosomal distribution, gene structure, conservative domains, cis-elements, phylogeny, and evolutionary relationship. We divided 28 CaGATAs into four groups distributed on 10 chromosomes, and identified 7 paralogs in CaGATA family of pepper and 35 orthologous gene pairs between CaGATAs and Arabidopsis GATAs (AtGATAs). The results of promoter cis-element analysis and the quantitative real-time PCR (qRT-PCR) analysis revealed that CaGATA genes were involved in regulating the plant growth and development and the responses to various abiotic stresses and hormone treatments in pepper. Tissue-specific expression analysis showed that most CaGATA genes were preferentially expressed in flower buds, flowers, and leaves. Several CaGATA genes, especially CaGATA14, were significantly regulated under multiple abiotic stresses, and CaGATA21 and CaGATA27 were highly responsive to phytohormone treatments. Taken together, our results lay a foundation for the biological function analysis of GATA gene family in pepper.


Asunto(s)
Capsicum/genética , Factores de Transcripción GATA , Hormonas/farmacología , Proteínas de Plantas , Estrés Fisiológico , Factores de Transcripción GATA/genética , Regulación de la Expresión Génica de las Plantas , Proteínas de Plantas/genética , Verduras
17.
Sci Rep ; 11(1): 10164, 2021 05 13.
Artículo en Inglés | MEDLINE | ID: mdl-33986344

RESUMEN

Trichomes, specialized epidermal cells located in aerial parts of plants, play indispensable roles in resisting abiotic and biotic stresses. However, the regulatory genes essential for multicellular trichrome development in Capsicum annuum L. (pepper) remain unclear. In this study, the transcript profiles of peppers GZZY-23 (hairy) and PI246331 (hairless) were investigated to gain insights into the genes responsible for the formation of multicellular trichomes. A total of 40,079 genes, including 4743 novel genes and 13,568 differentially expressed genes (DEGs), were obtained. Functional enrichment analysis revealed that the most noticeable pathways were transcription factor activity, sequence-specific DNA binding, and plant hormone signal transduction, which might be critical for multicellular trichome formation in hairy plants. We screened 11 DEGs related to trichome development; 151 DEGs involved in plant hormone signal transduction; 312 DEGs belonging to the MYB, bHLH, HD-Zip, and zinc finger transcription factor families; and 1629 DEGs predicted as plant resistance genes (PRGs). Most of these DEGs were highly expressed in GZZY-23 or trichomes. Several homologs of trichome regulators, such as SlCycB2, SlCycB3, and H, were considerably upregulated in GZZY-23, especially in the trichomes. The transcriptomic data generated in this study provide a basis for future characterization of trichome formation in pepper.


Asunto(s)
Capsicum/genética , Perfilación de la Expresión Génica/métodos , Secuenciación de Nucleótidos de Alto Rendimiento/métodos , Tricomas/genética , Capsicum/citología , Capsicum/crecimiento & desarrollo , Resistencia a la Enfermedad/genética , Regulación de la Expresión Génica de las Plantas , Sitios Genéticos , Fenotipo , Reguladores del Crecimiento de las Plantas/genética , Reguladores del Crecimiento de las Plantas/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Tallos de la Planta/citología , Tallos de la Planta/genética , Tallos de la Planta/crecimiento & desarrollo , Transducción de Señal/genética , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , Transcriptoma , Tricomas/citología , Tricomas/crecimiento & desarrollo
18.
PLoS One ; 16(3): e0240279, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33705404

RESUMEN

Bacterial spot (BS), incited by Xanthomonas campestris pv. vesicatoria (Xcv), is one of the most serious diseases of pepper. For a comparative analysis of defense responses to Xcv infection, we performed a transcriptomic analysis of a susceptible cultivar, ECW, and a resistant cultivar, VI037601, using the HiSeqTM 2500 sequencing platform. Approximately 120.23 G clean bases were generated from 18 libraries. From the libraries generated, a total of 38,269 expressed genes containing 11,714 novel genes and 11,232 differentially expressed genes (DEGs) were identified. Functional enrichment analysis revealed that the most noticeable pathways were plant-pathogen interaction, MAPK signaling pathway-plant, plant hormone signal transduction and secondary metabolisms. 1,599 potentially defense-related genes linked to pattern recognition receptors (PRRs), mitogen-activated protein kinase (MAPK), calcium signaling, and transcription factors may regulate pepper resistance to Xcv. Moreover, after Xcv inoculation, 364 DEGs differentially expressed only in VI037601 and 852 genes in both ECW and VI037601. Many of those genes were classified as NBS-LRR genes, oxidoreductase gene, WRKY and NAC transcription factors, and they were mainly involved in metabolic process, response to stimulus and biological regulation pathways. Quantitative RT-PCR of sixteen selected DEGs further validated the RNA-seq differential gene expression analysis. Our results will provide a valuable resource for understanding the molecular mechanisms of pepper resistance to Xcv infection and improving pepper resistance cultivars against Xcv.


Asunto(s)
Capsicum/genética , Perfilación de la Expresión Génica , Enfermedades de las Plantas/genética , Xanthomonas campestris/patogenicidad , Xanthomonas vesicatoria/patogenicidad , Capsicum/metabolismo , Capsicum/microbiología , Regulación hacia Abajo , Proteínas Quinasas Activadas por Mitógenos/genética , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Enfermedades de las Plantas/microbiología , Hojas de la Planta/genética , Hojas de la Planta/metabolismo , Hojas de la Planta/microbiología , Proteínas de Plantas/genética , ARN de Planta/química , ARN de Planta/metabolismo , Análisis de Secuencia de ARN , Transducción de Señal/genética , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , Regulación hacia Arriba
19.
Nanoscale ; 12(40): 20797-20810, 2020 Oct 22.
Artículo en Inglés | MEDLINE | ID: mdl-33034310

RESUMEN

Flexible all-solid-state supercapacitors (ASSSs) are excellent energy storage devices for portable/wearable electronics, although the development of an excellent comprehensive performance film electrode for the extraordinary flexible ASSSs still faces a great challenge. Here, bendable, foldable and anti-exfoliative Ti3C2Tx MXene-based films utilized as supercapacitor electrodes are reported. Polyaniline/Ti3C2Tx composites (i-PANI@Ti3C2Tx) were prepared by in situ oxidant-free polymerization of aniline on Ti3C2Tx nanosheets with p-phenylenediamine (PPD) as an initiator. Lignosulfonate (Lig) and Ti3C2Tx were constructed into a compact composite (Lig@Ti3C2Tx) film based on the hydrogen bonds formed between Lig and Ti3C2Tx. The Lig@Ti3C2Tx/i-PANI@Ti3C2Tx(5/5) hybrid film was produced by vacuum-assisted filtration of the mixed two composite dispersions. The as-prepared films can be arbitrarily deformed (such as bending and folding). They show high tensile strength and vertical-plane (the plane of film) tensile strength with 33.2 and 0.28 MPa for the i-PANI@Ti3C2Tx film, 75.4 and 0.77 MPa for the Lig@Ti3C2Tx film, and 53.7 and 0.58 MPa for the Lig@Ti3C2Tx/i-PANI@Ti3C2Tx(5/5) film (those of Ti3C2Tx film are 17.4 and 0.21 MPa), respectively. The enhanced vertical-plane tensile strength of the as-prepared composite films indicates that the large binding force generated between the Ti3C2Tx nanosheets can effectively prevent the exfoliation of films. The electrodes of the as-prepared i-PANI@Ti3C2Tx, Lig@Ti3C2Tx and Lig@Ti3C2Tx/i-PANI@Ti3C2Tx(5/5) films assembled into symmetric flexible ASSSs can deliver excellent specific capacitances of 310 F g-1 (∼1001 F cm-3), 271 F g-1 (∼881 F cm-3) and 295 F g-1 (∼959 F cm-3), respectively. In addition, the corresponding supercapacitors exhibit ultrahigh energy densities of 34.8, 30.6 and 33.3 W h L-1, respectively. It is expected that the as-prepared MXene-based films can be applied in various fields, such as electromagnetic-interference shielding and batteries. Furthermore, the as-prepared flexible ASSSs can be practically used as a wearable energy storage device.

20.
Front Plant Sci ; 11: 598798, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-33362830

RESUMEN

Cucumber mosaic virus (CMV) is a prevalent virus affecting the quality and yield of pepper, resulting in yield losses of greater than 80% during severe local epidemics. Cultural practices and the heavy use of agrochemicals are the most common control measures for CMV. Sources of resistance provide a practical reference and a basis for breeding for CMV resistance. Genetic factors underlying CMV resistance have been studied and advanced breeding lines and cultivars with improved resistance have been developed by traditional breeding methods. Additionally, QTLs or genes for CMV resistance have been identified and can be utilized for marker-assisted resistance breeding. This review focuses on status and prospect of CMV against different virus strains, host resistance, and its applied genetics. With the advent of novel technologies, more useful markers and precise approaches can facilitate the progress for improving CMV resistance in Capsicum.

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