Disturbance-Observer-Based U-Control (DOBUC) for Nonlinear Dynamic Systems.

U-model, which is a control-oriented model set with the property of generally facilitate nonlinearity dynamic inversion/cancellation, has been introduced to the Disturbance Observer-Based control (DOBC) methods to improve the performance of the nonlinear systems in this paper. A general DOB based U-Control (DOBUC) framework is proposed to improve the disturbance attenuation capability of U-controller for both linear and nonlinear systems combined with (based on) the U-model-based dynamic inversion which expands the classical linear disturbance observer control to general nonlinear systems. The proposed two-step DOBUC design procedures in which the design of DOB and U-controller are totally independent and separated, enables the establishment of global exponential stability without being subject to disturbances and uncertainties. Comparative simulation experiments with Nonlinear DOBC in controlling Wind Energy Conversion Systems (WECS) and Permanent Magnet Synchronous Motors (PMSM) demonstrated the proposed method.

FPGA design and implementation for EIT data acquisition.

OXBACT-5 was designed to meet the challenges involved in working in the intensive care hospital environment focussed particularly on thoracic imaging of patients with respiratory distress and chronic heart failure (CHF). The FPGA-based wireless LAN linked multi-channel EIT data acquisition system (DAS) providing 16 programmable excitation current channels and 64 voltage measurement channels is presented. It contains function modules of a PCI bus interface, direct digital synthesizers, dual-port memory blocks, digital demodulation and all the command and control logic in the FPGA. The whole EIT data acquisition system is fully programmable and reconfigurable from the host PC. The excitation frequency, excitation patterns, the measuring sequence and the gain of each measurement channel can be set from the host PC before each measurement. The demodulation is implemented in the FPGA chip to reduce the data rate between the DAS and the host PC. In addition, measurement process management is achieved in this FPGA chip. Complemented by analogue devices such as ADCs, DACs, analogue buffers and analogue multiplexers, the new FPGA-based EIT DAS system is implemented in a very compact way for bedside use in intensive care units of hospitals. It is intended for applications such as continuous respiration monitoring with data collection at 25 frames per second. Image reconstruction times depend on the choice of 2D or 3D imaging algorithms and the available processing power.

Intelligent bioprocessing for haemotopoietic cell cultures using monitoring and design of experiments.

The need for successful ex-vivo expansion and directed differentiation of haematopoietic stem cells (HSCs) for therapeutic applications has increased over the past decade. Haematopoietic cell cultures are complex and full characterisation of the process environment has yet to be achieved. The complexity and transient nature of HSC cultures make the identification, maintenance and control of optimal operating conditions challenging. Application of real-time, on-line monitoring techniques and process control strategies enhances the ability to operate bioprocesses of desired reproducibility and high product quality. In this review, we discussed the methods by which in vitro culture information necessary for bioprocess control may be obtained, including process considerations, monitoring and analytical tools, and design of experiments (DOE). The successful application of these tools may result in time- and cost-effective cultures for directed differentiation and expansion of haematopoietic components intended for clinical use.

Biocompatible ion selective electrode for monitoring metabolic activity during the growth and cultivation of human cells.

Ammonia is the main nitrogenous waste product of cellular metabolism and if accumulated in culture media may limit cell growth and affect the quality of cultured cell lines. Therefore, it is crucial to control levels of this metabolite during the in vitro expansion of human cells. This paper describes the successful application of ion selective electrodes (ISE) to continuously monitor ammonium concentrations in a perfused cell bioreactor. The polymeric membranes of the ISE were cast from carboxylated poly(vinyl chloride) (PVC-COOH) and doped with highly hydrophilic poly(ethylene glycol) (PEG). The PEG was incorporated into the surface of the sensors in order to reduce the effect of biofouling without impairing their analytical characteristics. The electrodes developed enabled fast and selective measurements of ammonia in the range 0.5-5mM, corresponding well with the concentration determined off-line. Additionally, the UV sterilised sensors were small and flexible enough to be readily inserted into the limited space of the bioreactor. Long-term analytical performance of PEG-modified ISE during continuous measurements in mammalian cell cultures was investigated. The sensors remained stable for the duration of the bioprocess, 7 days.