RESUMEN
PM2.5 can cause airway inflammation and promote the excessive secretion of mucin 5ac (Muc5ac), which can further induce many respiratory diseases. Antisense non-coding RNA in the INK4 locus (ANRIL) might regulate the inflammatory responses mediated by nuclear factor kappa-B (NF-κB) signaling pathway. Beas-2B cells were used to clarify the role of ANRIL in the secretion of Muc5ac induced by PM2.5 . The siRNA was used to silence ANRIL expression. Normal and gene silenced Beas-2B cells were respectively exposed to different doses of PM2.5 for 6, 12, and 24 h. The survival rate of Beas-2B cells was detected by methyl thiazolyl tetrazolium (MTT) assay. Tumor necrosis factor-α (TNF-α), interleukin-1ß (IL-1ß) and Muc5ac levels were determined by enzyme linked immunosorbent assay (ELISA). The expression levels of NF-κB family genes and ANRIL were detected by real time polymerase chain reaction (PCR). The levels of NF-κB family proteins and NF-κB family phosphorylated proteins were determined using Western blot. Immunofluorescence experiments were performed to observe the nuclear transposition of RelA. PM2.5 exposure increased the levels of Muc5ac, IL-1ß and TNF-α, and ANRIL gene expression (p < .05). With the dose and time of PM2.5 exposure increasing, the protein levels of inhibitory subunit of nuclear factor kappa-B alpha (IκB-α), RelA, and NF-κB1 decreased, the protein levels of phosphorylated RelA (p-RelA) and phosphorylated NF-κB1 (p-NF-κB1) increased, and RelA nuclear translocation increased, which indicated that the NF-κB signaling pathway was activated (p < .05). Silencing ANRIL could decrease the levels of Muc5ac, IL-1ß, TNF-α, decrease NF-κB family genes expression, inhibit the degradation of IκB-α and the activation of NF-κB pathway (p < .05). ANRIL played a regulatory role in the secretion of Muc5ac and the inflammation induced by atmospheric PM2.5 via NF-κB pathway in Beas-2B cells. ANRIL could be a target for prevention and treatment of the respiratory diseases caused by PM2.5 .
Asunto(s)
FN-kappa B , Factor de Necrosis Tumoral alfa , Humanos , FN-kappa B/metabolismo , Inhibidor NF-kappaB alfa/metabolismo , Factor de Necrosis Tumoral alfa/genética , Factor de Necrosis Tumoral alfa/metabolismo , Mucina 5AC/genética , Mucina 5AC/metabolismo , Células Epiteliales/metabolismo , Material Particulado/toxicidad , Inflamación/metabolismoRESUMEN
Angiogenesis refers to the formation of new blood vessels from existing blood vessels, including endothelial progenitor cells differentiation and cytokine regulation. Circular RNAs, a type of non-coding RNA, are a stable and conservative endogenous transcriptional product with a circular structure that is produced by the reverse and scrambled splicing of mRNA precursors. They can be used as microRNA sponges, are involved in transcription and protein translation, and regulate the pathophysiological processes of various diseases. Recent studies have shown that circRNAs can regulate angiogenesis by regulating vascular endothelial cell function. In this review, we summarize the angiogenic mechanism; the biogenesis, properties and biological function of circRNAs; and their roles in regulating angiogenesis. We also discuss their potential implications for clinical applications.
Asunto(s)
Enfermedades Cardiovasculares/metabolismo , Células Endoteliales/metabolismo , Endotelio Vascular/metabolismo , Neovascularización Fisiológica , ARN Circular/metabolismo , Animales , Enfermedades Cardiovasculares/genética , Enfermedades Cardiovasculares/fisiopatología , Células Progenitoras Endoteliales/metabolismo , Endotelio Vascular/fisiopatología , Regulación de la Expresión Génica , Humanos , Neovascularización Fisiológica/genética , ARN Circular/genética , Transducción de SeñalRESUMEN
BACKGROUND: Exposure to PM2.5 can stimulate the mucus secretion of airway, affecting the development of bronchial asthma. NF-κB signal pathway plays an important role in inflammation and dysimmunity, what may contribute to the mucus secretion. The present study was undertaken to explore the effect of NF-κB signal pathway on mucus secretion induced by PM2.5 in rats with bronchial asthma. METHODS: Fifty rats (25 males and 25 females) were divided randomly into the control group, ovalbumin asthmatic model group, asthma low-, middle- and high-dose groups (n = 10, 5 males and 5 females each group). The control group, ovalbumin asthmatic model group received physiological saline; the asthma low-, middle- and high-dose groups received 1.5, 7.5 and 37.5 mg/kg PM2.5 on saline, which instilled into the trachea at 2-day intervals for two doses. Lung histopathology was observed by HE staining. The mRNA levels of NF-κB family gens were detected with real time PCR. IκB-α protein expression levels were detected with Western blot. IL-1ß, TNF-α and Muc5ac levels were detected by ELISA. RESULTS: Respiratory mucus secretion increased with increasing dose of PM2.5. Compared with healthy rats, the protein expression levels of IκB-α were significantly lower in the lung of asthmatic rats (p < 0.05), while the relative mRNA expression levels of NF-κB family genes in tracheal tissue and in lung were significantly higher in the asthmatic rats (p < 0.05). Serum IL-1ß levels were significantly higher in the high-dose group than in the control group. Muc5ac protein levels in the trachea were higher in the high-dose compared with the low-and middle-dose groups. CONCLUSION: Short-term exposure to a high concentration of PM2.5 could up-regulate the mRNA expression levels of NF-κB family genes, activate the NF-κB signal pathway, stimulate more IL-1ß and mucus secretion in rats with bronchial asthma. NF-κB signal pathway may regulate the level of IL-1ß, which could influence the mucus secretion induced by PM2.5 in asthmatic rats.
Asunto(s)
Contaminantes Atmosféricos/toxicidad , FN-kappa B/metabolismo , Material Particulado/toxicidad , Animales , Asma/inmunología , Femenino , Inflamación/metabolismo , Pulmón/efectos de los fármacos , Masculino , Moco/metabolismo , Inhibidor NF-kappaB alfa/metabolismo , Ovalbúmina , Ratas , Transducción de Señal/efectos de los fármacos , Factor de Necrosis Tumoral alfa/metabolismoRESUMEN
BACKGROUND: Epidemiological studies have confirmed atmospheric PM2.5 could affect asthma, and dyslipidemia may be related to pathogenesis of asthma. Recent studies show Notch ligands had lipid combination domains which are responsible for regulating lipid levels. However, the effect of PM2.5 on asthmatic rats' lipid levels and the role of Notch signaling pathway is unclear. METHODS: Rats were treat with ovalbumin (OVA) to establish asthma models. Notch signaling pathway inhibitor (DAPT) was injected intraperitoneally. Asthmatic and healthy rats were exposed to different concentrations of PM2.5. Lung tissues were collected and the expression of Hes1 protein was detected by Western Blot. Blood samples were collected to detect the serum lipid levels. RESULTS: Hes1 expression levels in healthy and asthma pathway inhibition groups were lower than those in control groups. Compared with control group, rats exposed to PM2.5 in middle and high dose, the levels of TG and TC were decreased. Similar results were observed after exposure to the same concentration of PM2.5 in asthmatic rats. Rats, which were exposed to PM2.5 after being established the asthma model successfully, could exhibit more significant dyslipidemia than those with direct exposure. After Notch signaling pathway inhibited, TC and LDL in asthma pathway inhibition group were lower than those in healthy group. CONCLUSIONS: PM2.5 can affect the lipid levels of asthmatic rats through the Notch signaling pathway.
Asunto(s)
Asma/sangre , Dislipidemias/sangre , Expresión Génica/efectos de los fármacos , Material Particulado/administración & dosificación , Transducción de Señal/efectos de los fármacos , Factor de Transcripción HES-1/genética , Animales , Asma/inducido químicamente , Asma/genética , HDL-Colesterol/sangre , LDL-Colesterol/sangre , Diaminas/farmacología , Modelos Animales de Enfermedad , Dislipidemias/inducido químicamente , Dislipidemias/genética , Femenino , Metabolismo de los Lípidos/efectos de los fármacos , Masculino , Ovalbúmina , Material Particulado/antagonistas & inhibidores , Ratas , Ratas Wistar , Tiazoles/farmacología , Factor de Transcripción HES-1/metabolismo , Triglicéridos/sangreRESUMEN
BACKGROUND: Adolescence and young adulthood are critical periods of human growth and development. Phthalates are environmental endocrine disruptors, and their health hazards in adolescents and young adults cannot be ignored. This study was undertaken to assess phthalate exposure and determine the associations between lifestyle behaviors and phthalate metabolite levels in Chinese adolescents and young adults. METHODS: Four hundred and seventy-eight adolescents and young adults aged 16-20 years were included in this study. The levels of mono-ethyl phthalate (MEP), mono-butyl phthalate (MBP), mono-(2-ethylhexyl) phthalate (MEHP), mono-(2-ethyl-5-oxohexyl) phthalate (MEOHP), mono-(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP), mono-(2-ethyl-5-carboxypentyl) phthalate (MECPP) and mono-(2-carboxmethyl)-hexyl phthalate (MCMHP) in the subjects' urine were measured by high-performance liquid chromatography-tandem mass spectrometry. The estimated daily intake (EDI) and hazard index (HI) of phthalates were calculated based on urinary metabolite levels. Relevant information on the subjects was collected via questionnaires. The associations between phthalate metabolite levels and lifestyle behaviors were examined using the independent-sample t-test, Mann-Whitney test and multiple linear regression. RESULTS: In this study, the detection rates of all seven metabolites were >98%. The highest median metabolite concentration was MBP, which was 43.00⯵g/L (33.11⯵g/g creatinine). The highest median EDI was for di-(2-ethylhexyl) phthalate (DEHP), which was 2.40 µg/kg-bw/day (volume-based) and 1.51 µg/kg-bw/day (creatinine-based). 2.7% (volume-based) and 1.0% (creatinine-based) of the subjects showed excessive HITDI (HI of the tolerable daily intake) values, which indicated the cumulative risk of anti-androgenic effects. Furthermore, factors significantly associated with phthalate metabolite levels included the use of plastic food packages (DEHP metabolites), physical exercise (MEOHP), the frequency of fast food consumption (MBP), and the frequency of skin care cosmetics and color cosmetics use (MEP). CONCLUSION: Our results suggest that Chinese adolescents and young adults are widely exposed to phthalates and their metabolite levels are influenced by lifestyle behaviors.
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Conducta del Adolescente/efectos de los fármacos , Disruptores Endocrinos/orina , Exposición a Riesgos Ambientales/análisis , Conductas Relacionadas con la Salud/efectos de los fármacos , Estilo de Vida , Ácidos Ftálicos/orina , Adolescente , Adulto , China , Creatinina/orina , Estudios Transversales , Femenino , Humanos , Modelos Lineales , Masculino , Medición de Riesgo , Encuestas y Cuestionarios , Adulto JovenRESUMEN
With the development of information collection technology, the data that people need to deal with are also increasing, which brings problems such as isomerization of data types, poor data quality, and fast data generation speed. At present, as an important method of data fusion technology, data fusion method based on deep learning has become an effective way of data fusion under the background of big data, which has important research significance. There is a problem with heterogeneous data types between time series data and text data, and it is difficult to fuse them effectively by traditional data fusion methods. In order to make full use of the information contained in text data and improve the accuracy of time series prediction, this paper proposes a data fusion model based on FC-SAE. In this model, GloVe and CNN are used to extract the features of text data, FC neural network is used to extract the potential features of time series data, and then, the SEA model is used to fuse the data, which fully discovers the relationship between data and greatly improves the prediction accuracy.
Asunto(s)
Macrodatos , Internet de las Cosas , Algoritmos , Humanos , Redes Neurales de la Computación , TecnologíaRESUMEN
Angiogenesis is the process of growing endothelial capillary cells. Exosomes are extracellular vesicles that are rich in miRNAs. Studies have shown that exosomes can carry communication between cells and various tissues by delivering miRNAs to their target organs and cells. It has been repeatedly proven that miRNAs regulate the expression of growth factors and other proteins in endothelial cells through paracrine signalling and participate in the physiological and pathological processes of angiogenesis. In the diagnosis and treatment of diseases, exosome-derived microRNAs can play important roles as biomarkers and drug carriers. In this review, we introduce the characteristics of miRNAs and exosomes and their interactions. Then, we specifically summarize the exosome-derived miRNAs related to angiogenesis, and we discuss the potential uses of exosome-derived miRNAs for diagnosing and treating cardiovascular diseases. Graphical abstract.
Asunto(s)
Enfermedades Cardiovasculares , Exosomas , Vesículas Extracelulares , MicroARNs , Enfermedades Cardiovasculares/diagnóstico , Enfermedades Cardiovasculares/genética , Enfermedades Cardiovasculares/terapia , Células Endoteliales , Exosomas/genética , Humanos , MicroARNs/genéticaRESUMEN
Background: Nuclear factor-κB (NF-κB) signaling plays essential roles in inflammatory responses. However, whether the expression levels of NF-κB family genes affect inflammatory responses is unclear. Moreover, little is known regarding the association between NF-κB family genes expression and the pathogenesis of chronic obstructive pulmonary disease (COPD). The present study was undertaken to assess the relationship between the expression levels of NF-κB family genes mRNA and of inflammatory markers relevant to COPD pathogenesis. Methods: A total of 186 unrelated patients with acute exacerbations of COPD and 180 healthy controls were recruited. Total RNA was extracted from the peripheral fasting blood of each subject using trizol reagent. The mRNA levels of NF-κB family genes (NF-κB1, NF-κB2 and c-REL) were measured by real-time quantitative polymerase chain reaction. The serum levels of cyclooxygenase-2 (COX-2), C-reactive protein, interleukin (IL)-1ß, IL-6, IL-8, IL-12 and tumor necrosis factor-α were measured with enzyme-linked immunosorbent assay kits. Results: The relative mRNA levels of the NF-κB family genes and the levels of inflammatory molecules were significantly higher in the COPD group than in the control group after adjustment for smoking. The IL-1ß, IL-8 and COX-2 levels were significantly lower in the NF-κB2 under-expression subgroup as compared to the NF-κB2 over-expression subgroup. The COX-2 level was significantly lower (P < 0.05) in the c-REL under-expression subgroup as compared to the c-REL over-expression subgroup. Conclusion: NF-κB2 over-expression was associated with IL-1ß, IL-8 and COX-2 levels, whereas c-REL overexpression was associated with COX-2 level. Over-expression of both NF-κB2 and c-REL was found to be related to COPD.
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Expresión Génica , Genes rel , Subunidad p50 de NF-kappa B/genética , Subunidad p52 de NF-kappa B/genética , Enfermedad Pulmonar Obstructiva Crónica/genética , Proteína C-Reactiva/análisis , Estudios de Casos y Controles , Ciclooxigenasa 2/sangre , Progresión de la Enfermedad , Femenino , Humanos , Interleucina-1beta/sangre , Interleucinas/sangre , Masculino , Persona de Mediana Edad , Enfermedad Pulmonar Obstructiva Crónica/sangre , ARN Mensajero/genética , ARN Mensajero/metabolismo , Factor de Necrosis Tumoral alfa/sangreRESUMEN
As a plasticizer, di-(2-ethylhexyl)-phthalate (DEHP) is widely added in various commercial products. Some researchers had suggested that DEHP has adverse effects on the glucose metabolism, but the mechanisms remain unclear. Adolescent Wistar rats were divided into four groups and administered DEHP by gavage at 0, 5, 50, and 500 mg kg-1 d-1 for 28 days. ELISA was used to quantify the serum insulin and leptin levels; RT-PCR, immunohistochemistry, and Western blot were used to detect the mRNA and protein expressions of Janus-activated kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3), suppressor of cytokine signaling 3 (SOCS3), leptin receptor (Ob-R), and insulin receptor (IR) in liver and pancreas In comparison to the control group, the DEHP-treated rats showed the following: (1) higher organ coefficient of liver; (2) higher fasting blood glucose levels, higher fasting serum insulin and leptin levels, higher insulin resistance index homeostasis model assessment; (3) lower protein levels of Ob-R and IR in the liver and pancreas; (4) higher protein levels of JAK2 and STAT3 in the liver; and (5) higher protein and mRNA levels of SOCS3 in the liver and pancreas. Exposure to DEHP induced glucose metabolic disorder in the adolescent rats, and the mechanism is that DEHP may interfere with the JAK2/STAT3/SOCS3 pathway, regulated the sensitivity of the insulin receptor and leptin receptor.
Asunto(s)
Dietilhexil Ftalato/toxicidad , Trastornos del Metabolismo de la Glucosa/inducido químicamente , Plastificantes/toxicidad , Animales , Glucemia/metabolismo , Trastornos del Metabolismo de la Glucosa/metabolismo , Insulina/sangre , Resistencia a la Insulina , Leptina/sangre , Hígado/efectos de los fármacos , Hígado/metabolismo , Masculino , Ratas , Ratas Wistar , Receptor de Insulina/metabolismo , Receptores de Leptina/metabolismoRESUMEN
Exposure to PM2.5 induces systemic inflammation, and the NF-κB signaling pathway plays an important role in the inflammation process. We aim to clarify whether the expression of NF-κB gene family affects inflammation caused by PM2.5. Human monocytic cells (THP-1) were induced to differentiate into macrophages using phorbol myristate acetate. The macrophages were then treated with 100, 200, and 400 µg/ml of PM2.5 for 12, 24, and 48 h, respectively. Then, we determined the survival rate of macrophages through the MTT assay. The TNF-α and CRP levels in the cell culture medium were measured through enzyme-linked immunosorbent assay. The NF-κB1, NF-κB2, RelA, RelB, and Rel mRNA levels in macrophages were measured with reverse transcriptase-polymerase chain reaction. As a consequence, the survival rate of macrophages decreased with increasing PM2.5 exposure time and dose. The TNF-α levels in PM2.5-treated groups were lower as compared with the control group and in contrast to the NF-κB mRNA levels at all exposure times. The TNF-α level in the 400-µg/ml group and the NF-κB1, NF-κB2, RelB, and Rel mRNA levels in all PM2.5-treated groups were found to be higher at 24 h than at 12 h. Furthermore, the TNF-α, CRP, and NF-κB2 mRNA levels in the group treated with 400 µg/ml PM2.5 were higher at 48 h that at 12 and 24 h. On the other hand, the NF-κB1, RelA, RelB, and Rel mRNA levels in all PM2.5-treated groups were lower as compared to levels of TNF-α, CRP, and NF-κB2 mRNA. The levels of NF-κB genes and inflammatory cytokines demonstrated different correlations at different exposure times. Therefore, we conclude that PM2.5 reduces the survival rate of macrophages. As macrophages are exposed to PM2.5, the NF-κB gene family expression is increased, which subsequently affects inflammatory factor levels.
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Contaminantes Atmosféricos/farmacología , Citocinas/metabolismo , Macrófagos/metabolismo , FN-kappa B/genética , Material Particulado/farmacología , Línea Celular , Supervivencia Celular/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Humanos , Inflamación/etiología , Inflamación/metabolismo , Macrófagos/citología , FN-kappa B/metabolismoRESUMEN
The most widely used plasticizer, di-(2-ethylhexyl) phthalate (DEHP), is known to affect lipid metabolism and adipogenesis. We studied the effects of dietary DEHP exposure on metabolism in rats as well as the role of the JAK/STAT pathway in this process. Eighty rats were exposed to DEHP (0, 5, 50 and 500 mg/kg/d) through dietary intake for 4 weeks. We then collected blood samples, liver, and adipose tissues to detect modifications in the levels of serum lipids, leptin, adiponectin and insulin. JAK3, STAT5a and PPARγ expression were detected at both the gene and protein levels. The activation of JAK3 and STAT5a was also detected. The DEHP-exposed rats had increased body weight, serum lipid, insulin, and leptin levels. Moreover, the JAK3/STAT5a pathway was activated in the adipose tissue; however, this pathway was not activated in the liver. The mRNA of SREBP-1c in the liver was increased significantly among each of the groups, in contrast to the levels found in the mature SREBP-1c protein form. Furthermore, the expression of FABP4, Acox and FASn was decreased in the liver, but increased in adipose tissue. Thus, we conclude that exposure to DEHP reduces the hydrolysis of lipid and promotes triglyceride accumulation by oppositely regulating the activation state of JAK/STAT pathway in the liver and adipose tissue, resulting in the disorder of body lipid metabolism and obesity.