RESUMEN
The transthoracic puncture biopsy was performed under surveillance of X-ray computed tomography in order to verify diagnoses in 80 patients with lung tumors. This procedure allowed verification of diagnosis and choice of treatment strategy in 95% of cases. The complications obtained as result of intervention didn't bring severe character and were arrested in term of two days.
Asunto(s)
Biopsia con Aguja/métodos , Biopsia Guiada por Imagen/métodos , Neoplasias Pulmonares , Pulmón , Diagnóstico Diferencial , Femenino , Humanos , Pulmón/diagnóstico por imagen , Pulmón/patología , Neoplasias Pulmonares/diagnóstico , Neoplasias Pulmonares/patología , Masculino , Persona de Mediana Edad , Procedimientos Quirúrgicos Torácicos/métodos , Tomografía Computarizada por Rayos X/métodosAsunto(s)
Trasplante de Corazón/efectos adversos , Terapia de Inmunosupresión/efectos adversos , Mediastinitis , Osteomielitis , Complicaciones Posoperatorias , Esternotomía/efectos adversos , Esternón , Técnicas de Cierre de Heridas , Adulto , Cardiomiopatía Dilatada/complicaciones , Insuficiencia Cardíaca/etiología , Insuficiencia Cardíaca/cirugía , Trasplante de Corazón/métodos , Humanos , Terapia de Inmunosupresión/métodos , Masculino , Mediastinitis/diagnóstico , Mediastinitis/etiología , Mediastinitis/terapia , Colgajo Miocutáneo , Osteomielitis/diagnóstico , Osteomielitis/etiología , Osteomielitis/cirugía , Complicaciones Posoperatorias/diagnóstico , Complicaciones Posoperatorias/etiología , Complicaciones Posoperatorias/cirugía , Reoperación/métodos , Esternón/diagnóstico por imagen , Esternón/patología , Esternón/cirugía , Tomografía Computarizada Espiral , Resultado del TratamientoRESUMEN
Urine of prostate cancer (PCa) carries miRNAs originated from prostate cancer cells as a part of both nucleoprotein complexes and cell-secreted extracellular vesicles. The analysis of such miRNA-markers in urine can be a convenient option for PCa screening. The aims of this study were to reveal miRNA-markers of PCa in urine and design a robust and precise diagnostic test, based on miRNA expression analysis. The expression analysis of the 84 miRNAs in paired urine extracellular vesicles (EVs) and cell free urine supernatant samples from healthy donors, patients with benign and malignant prostate tumours was done using miRCURY LNA miRNA qPCR Panels (Exiqon, Denmark). Sets of miRNAs differentially expressed between the donor groups were found in urine EVs and urine supernatant. Diagnostically significant miRNAs were selected and algorithm of data analysis, based on expression data on 24-miRNA in urine and obtained using 17 analytical systems, was designed. The developed algorithm of data analysis describes a series of steps necessary to define cut-off values and sequentially analyze miRNA expression data according to the cut-offs to facilitate classification of subjects in case/control groups and allows to detect PCa patients with 97.5% accuracy.
Asunto(s)
MicroARNs/genética , Neoplasias de la Próstata/diagnóstico , Neoplasias de la Próstata/genética , Anciano , Anciano de 80 o más Años , Algoritmos , Biomarcadores de Tumor/genética , Biomarcadores de Tumor/orina , Estudios de Casos y Controles , Interpretación Estadística de Datos , Vesículas Extracelulares/genética , Redes Reguladoras de Genes , Humanos , Masculino , MicroARNs/orina , Persona de Mediana Edad , Hiperplasia Prostática/diagnóstico , Hiperplasia Prostática/genética , Hiperplasia Prostática/orina , Neoplasias de la Próstata/orinaRESUMEN
Urine of prostate cancer patients contains tumor-specific biopolymers, including protein- and microvesiclesassociated miRNAs that can potentially be used as oncomarkers. Previously we have characterized urine extracellular vesicles and demonstrated diagnostic potential of their miRNA cargo. In this study, we have performed a comparative analysis of the expression of 84 miRNA in paired samples of urine microvesicles and clarified urine from healthy men, patients with benign hyperplasia and cancer of the prostate using miRCURY LNA miRNA qPCR Panels. Subsets of miRNAs with differences in expression between the fractions of the urine were found in all three groups. Two groups of miRNA were identified based on the patterns of their differential expression. They regulate several key signaling pathways associated with prostate cancer development.
Asunto(s)
Líquidos Corporales , Micropartículas Derivadas de Células , Vesículas Extracelulares , Neoplasias de la Próstata , Humanos , Masculino , MicroARNsRESUMEN
Spectrometry has been employed to assess the levels of collagenase, catepsin D, trypsin-like proteinases and their inhibitors as well as bone acid and alkaline phosphatase both in the center and along the periphery of giant cell tumor of bone (GCTB) and chondrosarcoma. The levels of collagenase, trypsin-like proteinases and their inhibitors in the center of chondrosarcoma were much higher while those of alkaline phosphatase--lower than along tumor periphery. The catepsin D and acid phosphatase concentrations of the center and periphery of chondrosarcoma were similar. It was suggested that an extremely low concentration of trypsin-like inhibitors may contribute to degradation of the matrix in tissues adjacent to chondrosarcoma and, consequently, to tumor invasion development.