Development of a robotic cluster for automated and scalable cell therapy manufacturing.

BACKGROUND AIMS: The production of commercial autologous cell therapies such as chimeric antigen receptor T cells requires complex manual manufacturing processes. Skilled labor costs and challenges in manufacturing scale-out have contributed to high prices for these products. METHODS: We present a robotic system that uses industry-standard cell therapy manufacturing equipment to automate the steps involved in cell therapy manufacturing. The robotic cluster consists of a robotic arm and customized modules, allowing the robot to manipulate a variety of standard cell therapy instruments and materials such as incubators, bioreactors, and reagent bags. This system enables existing manual manufacturing processes to be rapidly adapted to robotic manufacturing, without having to adopt a completely new technology platform. Proof-of-concept for the robotic cluster's expansion module was demonstrated by expanding human CD8+ T cells. RESULTS: The robotic cultures showed comparable cell yields, viability, and identity to those manually performed. In addition, the robotic system was able to maintain culture sterility. CONCLUSIONS: Such modular robotic solutions may support scale-up and scale-out of cell therapies that are developed using classical manual methods in academic laboratories and biotechnology companies. This approach offers a pathway for overcoming manufacturing challenges associated with manual processes, ultimately contributing to the broader accessibility and affordability for personalized immunotherapies.

Edge IoT Prototyping Using Model-Driven Representations: A Use Case for Smart Agriculture.

Industry 4.0 is positioned at the junction of different disciplines, aiming to re-engineer processes and improve effectiveness and efficiency. It is taking over many industries whose traditional practices are being disrupted by advances in technology and inter-connectivity. In this context, enhanced agriculture systems incorporate new components that are capable of generating better decision making (humidity/temperature/soil sensors, drones for plague detection, smart irrigation, etc.) and also include novel processes for crop control (reproducible environmental conditions, proven strategies for water stress, etc.). At the same time, advances in model-driven development (MDD) simplify software development by introducing domain-specific abstractions of the code that makes application development feasible for domain experts who cannot code. XMDD (eXtreme MDD) makes this way to assemble software even more user-friendly and enables application domain experts who are not programmers to create complex solutions in a more straightforward way. Key to this approach is the introduction of high-level representations of domain-specific functionalities (called SIBs, service-independent building blocks) that encapsulate the programming code and their organisation in reusable libraries, and they are made available in the application development environment. This way, new domain-specific abstractions of the code become easily comprehensible and composable by domain experts. In this paper, we apply these concepts to a smart agriculture solution, producing a proof of concept for the new methodology in this application domain to be used as a portable demonstrator for MDD in IoT and agriculture in the Confirm Research Centre for Smart Manufacturing. Together with model-driven development tools, we leverage here the capabilities of the Nordic Thingy:53 as a multi-protocol IoT prototyping platform. It is an advanced sensing device that handles the data collection and distribution for decision making in the context of the agricultural system and supports edge computing. We demonstrate the importance of high-level abstraction when adopting a complex software development cycle within a multilayered heterogeneous IT ecosystem.

Innovative Metaheuristic Optimization Approach with a Bi-Triad for Rehabilitation Exoskeletons.

The present work proposes a comprehensive metaheuristic methodology for the development of a medical robot for the upper limb rehabilitation, which includes the topological optimization of the device, kinematic models (5 DOF), human-robot interface, control and experimental tests. This methodology applies two cutting-edge triads: (1) the three points of view in engineering design (client, designer and community) and (2) the triad formed by three pillars of Industry 4.0 (autonomous machines and systems, additive manufacturing and simulation of virtual environments). By applying the proposed procedure, a robotic mechanism was obtained with a reduction of more than 40% of its initial weight and a human-robot interface with three modes of operation and a biomechanically viable kinematic model for humans. The digital twin instance and its evaluation through therapeutic routines with and without disturbances was assessed; the average RMSEs obtained were 0.08 rad and 0.11 rad, respectively. The proposed methodology is applicable to any medical robot, providing a versatile and effective solution for optimizing the design and development of healthcare devices. It adopts an innovative and scalable approach to enhance their processes.

Advanced Monitoring of Manufacturing Process through Video Analytics.

The digitization of production systems has revolutionized industrial monitoring. Analyzing real-time bottom-up data enables the dynamic monitoring of industrial processes. Data are collected in various types, like video frames and time signals. This article focuses on leveraging images from a vision system to monitor the manufacturing process on a computer numerical control (CNC) lathe machine. We propose a method for designing and integrating these video modules on the edge of a production line. This approach detects the presence of raw parts, measures process parameters, assesses tool status, and checks roughness in real time using image processing techniques. The efficiency is evaluated by checking the deployment, the accuracy, the responsiveness, and the limitations. Finally, a perspective is offered to use the metadata off the edge in a more complex artificial-intelligence (AI) method for predictive maintenance.

Digital Twin of a Water Supply System Using the Asset Administration Shell.

The concept of digital twins is one of the fundamental pillars of Industry 4.0. Digital twin allows the realization of a virtual model of a real system, enhancing the relevant performance (e.g., in terms of production rate, risk prevention, energy saving, and maintenance operation). Current literature presents many contributions pointing out the advantages that may be achieved by the definition of a digital twin of a water supply system. The Reference Architecture Model for Industry 4.0 introduces the concept of the Asset Administration Shell for the digital representation of components within the Industry 4.0 ecosystem. Several proposals are currently available in the literature considering the Asset Administration Shell for the realization of a digital twin of real systems. To the best of the authors' knowledge, at the moment, the adoption of Asset Administration Shell for the digital representation of a water supply system is not present in the current literature. For this reason, the aim of this paper is to present a methodological approach for developing a digital twin of a water supply system using the Asset Administration Shell metamodel. The paper will describe the approach proposed by the author and the relevant model based on Asset Administration Shell, pointing out that its implementation is freely available on the GitHub platform.

Hydrogen 4.0: A Cyber-Physical System for Renewable Hydrogen Energy Plants.

The demand for green hydrogen as an energy carrier is projected to exceed 350 million tons per year by 2050, driven by the need for sustainable distribution and storage of energy generated from sources. Despite its potential, hydrogen production currently faces challenges related to cost efficiency, compliance, monitoring, and safety. This work proposes Hydrogen 4.0, a cyber-physical approach that leverages Industry 4.0 technologies-including smart sensing, analytics, and the Internet of Things (IoT)-to address these issues in hydrogen energy plants. Such an approach has the potential to enhance efficiency, safety, and compliance through real-time data analysis, predictive maintenance, and optimised resource allocation, ultimately facilitating the adoption of renewable green hydrogen. The following sections break down conventional hydrogen plants into functional blocks and discusses how Industry 4.0 technologies can be applied to each segment. The components, benefits, and application scenarios of Hydrogen 4.0 are discussed while how digitalisation technologies can contribute to the successful integration of sustainable energy solutions in the global energy sector is also addressed.

IOTA-Based Distributed Ledger in the Mining Industry: Efficiency, Sustainability and Transparency.

The paper presents a traceability framework founded upon a methodological approach specifically designed for the integration of the IOTA-based distributed ledger within the mining industry. This framework constitutes an initial stride towards the certification and labelling of sustainable material production. The efficacy of this methodology is subject to real-world evaluation within the framework of the European Commission funded project DIG_IT. Within the architectural framework, the integration of decentralized identifiers (DIDs) and the IOTA network are instrumental in effecting the encryption of data records, with associated hashes securely anchored on the explorer. Recorded environmental parameters, encompassing metrics such as pH level, turbidity, electrical conductivity, and emissions, serve as tangible evidence affirming their adherence to prevailing regulatory standards. The overarching system architecture encompasses a sophisticated Industrial Internet of Things platform (IIoTp), facilitating the seamless connection of data from a diverse array of sensors. End users, including governmental entities, mining managers, and the general public, stand to derive substantial benefits from tailored dashboards designed to facilitate the validation of data for emission compliance.

An Approach to Develop Digital Twins in Industry.

The industry is currently undergoing a digital revolution driven by the integration of several enabling technologies. These include automation, robotics, cloud computing, industrial cybersecurity, systems integration, digital twins, etc. Of particular note is the increasing use of digital twins, which offer significant added value by providing realistic and fully functional process simulations. This paper proposes an approach for developing digital twins in industrial environments. The novelty lies in not only focusing on obtaining the model of the industrial system and integrating virtual reality and/or augmented reality but also in emphasizing the importance of incorporating other enabled technologies of Industry 4.0, such as system integration, connectivity with standard and specific industrial protocols, cloud services, or new industrial automation systems, to enhance the capabilities of the digital twin. Furthermore, a proposal of the software tools that can be used to achieve this incorporation is made. Unity is chosen as the real-time 3D development tool for its cross-platform capability and streamlined industrial system modeling. The integration of augmented reality is facilitated by the Vuforia SDK. Node-RED is selected as the system integration option, and communications are carried out with MQTT protocol. Finally, cloud-based services are recommended for effective data storage and processing. Furthermore, this approach has been used to develop a digital twin of a robotic electro-pneumatic cell.

Novel Framework for Quality Control in Vibration Monitoring of CNC Machining.

Vibrations are a common issue in the machining and metal-cutting sector, in which the spindle vibration is primarily responsible for the poor surface quality of workpieces. The consequences range from the need to manually finish the metal surfaces, resulting in time-consuming and costly operations, to high scrap rates, with the corresponding waste of time and resources. The main problem of conventional solutions is that they address the suppression of machine vibrations separately from the quality control process. In this novel proposed framework, we combine advanced vibration-monitoring methods with the AI-driven prediction of the quality indicators to address this problem, increasing the quality, productivity, and efficiency of the process. The evaluation shows that the number of rejected parts, time devoted to reworking and manual finishing, and costs are reduced considerably. The framework adopts a generalized methodology to tackle the condition monitoring and quality control processes. This allows for a broader adaptation of the solutions in different CNC machines with unique setups and configurations, a challenge that other data-driven approaches in the literature have found difficult to overcome.

Convolutional Neural Networks for Raw Signal Classification in CNC Turning Process Monitoring.

This study addresses the need for advanced machine learning-based process monitoring in smart manufacturing. A methodology is developed for near-real-time part quality prediction based on process-related data obtained from a CNC turning center. Instead of the manual feature extraction methods typically employed in signal processing, a novel one-dimensional convolutional architecture allows the trained model to autonomously extract pertinent features directly from the raw signals. Several signal channels are utilized, including vibrations, motor speeds, and motor torques. Three quality indicators-average roughness, peak-to-valley roughness, and diameter deviation-are monitored using a single model, resulting in a compact and efficient classifier. Training data are obtained via a small number of experiments designed to induce variability in the quality metrics by varying feed, cutting speed, and depth of cut. A sliding window technique augments the dataset and allows the model to seamlessly operate over the entire process. This is further facilitated by the model's ability to distinguish between cutting and non-cutting phases. The base model is evaluated via k-fold cross validation and achieves average F1 scores above 0.97 for all outputs. Consistent performance is exhibited by additional instances trained under various combinations of design parameters, validating the robustness of the proposed methodology.

Improving the Concrete Crack Detection Process via a Hybrid Visual Transformer Algorithm.

Inspections of concrete bridges across the United States represent a significant commitment of resources, given their biannual mandate for many structures. With a notable number of aging bridges, there is an imperative need to enhance the efficiency of these inspections. This study harnessed the power of computer vision to streamline the inspection process. Our experiment examined the efficacy of a state-of-the-art Visual Transformer (ViT) model combined with distinct image enhancement detector algorithms. We benchmarked against a deep learning Convolutional Neural Network (CNN) model. These models were applied to over 20,000 high-quality images from the Concrete Images for Classification dataset. Traditional crack detection methods often fall short due to their heavy reliance on time and resources. This research pioneers bridge inspection by integrating ViT with diverse image enhancement detectors, significantly improving concrete crack detection accuracy. Notably, a custom-built CNN achieves over 99% accuracy with substantially lower training time than ViT, making it an efficient solution for enhancing safety and resource conservation in infrastructure management. These advancements enhance safety by enabling reliable detection and timely maintenance, but they also align with Industry 4.0 objectives, automating manual inspections, reducing costs, and advancing technological integration in public infrastructure management.

IIoT Low-Cost ZigBee-Based WSN Implementation for Enhanced Production Efficiency in a Solar Protection Curtains Manufacturing Workshop.

Nowadays, the Industry 4.0 concept and the Industrial Internet of Things (IIoT) are considered essential for the implementation of automated manufacturing processes across various industrial settings. In this regard, wireless sensor networks (WSN) are crucial due to their inherent mobility, easy deployment and maintenance, scalability, and low power consumption, among other benefits. In this context, the presented paper proposes an optimized and low-cost WSN based on ZigBee communication technology for the monitoring of a real manufacturing facility. The company designs and manufactures solar protection curtains and aims to integrate the deployed WSN into the Enterprise Resource Planning (ERP) system in order to optimize their production processes and enhance production efficiency and cost estimation capabilities. To achieve this, radio propagation measurements and 3D ray launching simulations were conducted to characterize the wireless channel behavior and facilitate the development of an optimized WSN system that can operate in the complex industrial environment presented and validated through on-site wireless channel measurements, as well as interference analysis. Then, a low-cost WSN was implemented and deployed to acquire real-time data from different machinery and workstations, which will be integrated into the ERP system. Multiple data streams have been collected and processed from the shop floor of the factory by means of the prototype wireless nodes implemented. This integration will enable the company to optimize its production processes, fabricate products more efficiently, and enhance its cost estimation capabilities. Moreover, the proposed system provides a scalable platform, enabling the integration of new sensors as well as information processing capabilities.

Advancing Digital Twin-Based Collision Avoidance: A Comprehensive Analysis of Communication Networks for Safety-Critical Applications in Industry 4.0.

This study presents a theoretical framework for defining the performance level of wireless safety functions within industrial environments. While acknowledging the simplifications inherent in our approach-primarily based on packet loss rates as a measure of system performance-the study underscores the dynamic challenges posed by real-world warehouses. Through an in situ measurement study of a forklift truck safety system, we validate the proposed method and emphasize the need for a more nuanced examination of wireless communication in complex settings. The study advocates for an expanded theoretical framework that considers fluctuations in warehouse dynamics, accounting for their impact on packet loss rates and, consequently, the precision of performance-level assessments. Furthermore, the research highlights the complexity introduced by wireless system characteristics not addressed in the simplified model, urging future investigations to incorporate these factors for a comprehensive understanding of wireless safety systems. The absence of specific criteria for wireless systems within existing standards emphasizes the necessity for a specialized framework in addressing safety aspects unique to wireless applications.

Recent Development of Air Gauging in Industry 4.0 Context.

The paper presents a review of the research reports published in 2012-2022, dedicated to air gauging. Since most of the results are somehow related to Industry 4.0 concept, the review put the air gauging to the context of fourth industrial revolution. It was found that despite substantial decrease of the number of published papers in recent years, the investigations are still performed to improve air gauges, both in static and in non-steady states. Researchers paid attention to the digitization of the results, models and simulations, uncertainty estimation, calibration, and linearization. Specific applications covered real-time monitoring and in-process control, as well as form and surface topography measurements. Proposed solutions for integration with computer systems seem suitable for the air gauges be included to the sensor networks built according to the Industry 4.0 concept.

How Industry 4.0 and Sensors Can Leverage Product Design: Opportunities and Challenges.

The fourth industrial revolution, also known as Industry 4.0, has led to an increased transition towards automation and reliance on data-driven innovations and strategies. The interconnected systems and processes have significantly increased operational efficiency, enhanced organizational capacity to monitor and control functions, reduced costs, and improved product quality. One significant way that companies have achieved these benefits is by integrating diverse sensor technologies within these innovations. Given the rapidly changing market conditions, Industry 4.0 requires new products and business models to ensure companies adjust to the current and future changes. These requirements call for the evolutions in product design processes to accommodate design features and principles applicable in the current dynamic business environment. Thus, it becomes imperative to understand how these innovations can leverage product design to maximize benefits and opportunities. This research paper employs a Systematic Literature Review with Bibliometric Analysis (SLBA) methodology to explore and synthesize data on how Industry 4.0 and sensors can leverage product design. The results show that various product design features create opportunities to be leveraged to guarantee the success of Industry 4.0 and sensor technologies. However, the research also identifies numerous challenges that undermine the ongoing transition towards intelligent factories and products.

Autoencoders Based on 2D Convolution Implemented for Reconstruction Point Clouds from Line Laser Sensors.

Gradual development is moving from standard visual content in the form of 2D data to the area of 3D data, such as points scanned by laser sensors on various surfaces. An effort in the field of autoencoders is to reconstruct the input data based on a trained neural network. For 3D data, this task is more complicated due to the demands for more accurate point reconstruction than for standard 2D data. The main difference is in shifting from discrete values in the form of pixels to continuous values obtained by highly accurate laser sensors. This work describes the applicability of autoencoders based on 2D convolutions for 3D data reconstruction. The described work demonstrates various autoencoder architectures. The reached training accuracies are in the range from 0.9447 to 0.9807. The obtained values of the mean square error (MSE) are in the range from 0.059413 to 0.015829 mm. They are close to resolution in the Z axis of the laser sensor, which is 0.012 mm. The improvement of reconstruction abilities is reached by extracting values in the Z axis and defining nominal coordinates of points for the X and Y axes, where the structural similarity metric value is improved from 0.907864 to 0.993680 for validation data.

Towards the Augmentation of Digital Twin Performance.

Digital Twin (DT) aims to provide industrial companies with an interface to visualize, analyze, and simulate the production process, improving overall performance. This paper proposes to extend existing DT by adding a complementary methodology to make it suitable for process supervision. To implement our methodology, we introduce a novel framework that identifies, collects, and analyses data from the production system, enhancing DT functionalities. In our case study, we implemented Key Performance Indicators (KPIs) in the immersive environment to monitor physical processes through cyber representation. First, a review of the Digital Twin (DT) allows us to understand the status of the existing methodologies as well as the problem of data contextualization in recent years. Based on this review, performance data in Cyber-Physical Systems (CPS) are identified, localized, and processed to generate indicators for monitoring machine and production line performance through DT. Finally, a discussion reveals the difficulties of integration and the possibilities to respond to other major industrial challenges, like predictive maintenance.

A Cost-Benefit Analysis Simulation for the Digitalisation of Cold Supply Chains.

This paper investigates using simulation to predict the benefits and costs of digitalising cold distribution chains. The study focuses on the distribution of refrigerated beef in the UK, where digitalisation was implemented to re-route cargo carriers. By comparing simulations of both digitalised and non-digitalised supply chains, the study found that digitalisation can reduce beef waste and decrease the number of miles driven per successful delivery, leading to potential cost savings. Note that this work is not attempting to prove that digitalisation is appropriate for the chosen scenario, only to justify a simulation approach as a decision making tool. The proposed modelling approach provides decision-makers with more accurate predictions of the cost-benefit of increased sensorisation in supply chains. By accounting for stochastic and variable parameters, such as weather and demand fluctuations, simulation can be used to identify potential challenges and estimate the economic benefits of digitalisation. Moreover, qualitative assessments of the impact on customer satisfaction and product quality can help decision-makers consider the broader impacts of digitalisation. Overall, the study suggests that simulation can play a crucial role in facilitating informed decisions about the implementation of digital technologies in the food supply chain. By providing a better understanding of the potential costs and benefits of digitalisation, simulation can help organisations make more strategic and effective decisions.

Blockchain-Internet of Things Applications: Opportunities and Challenges for Industry 4.0 and Society 5.0.

Today, blockchain is becoming more popular in academia and industry because it is a distributed, decentralised technology which is changing many industries in terms of security, building trust, etc. A few blockchain applications are banking, insurance, logistics, transportation, etc. Many insurance companies have been thinking about how blockchain could help them be more efficient. There is still a lot of hype about this immutable technology, even though it has not been utilised to its full potential. Insurers have to decide whether or not to use blockchain, just like many other businesses do. This technology keeps a distributed ledger on each blockchain node, making it more secure and transparent. The blockchain network can operate smart contracts and convince others to agree, so criminals cannot make mistakes. On another side, the Internet of Things (IoT) might make a real-time application work faster through its automation. With the integration of blockchain and IoT, there will always be a problem with technology regarding IoT devices and mining the blockchain. This paper gives a real-time view of blockchain-IoT-based applications for Industry 4.0 and Society 5.0. The last few sections discuss essential topics such as open issues, challenges, and research opportunities for future researchers to expand research in blockchain-IoT-based applications.

Research on Impact of IoT on Warehouse Management.

Automation and digitisation are the driving force of the Industrial Revolution 4.0. Industrial revolutions led to the mass production of goods, which increased the need for modern warehouses. Every year, the operation of warehouses becomes increasingly more complicated due to the increasing abundance of goods, thus the usual warehouse management strategies are no longer suitable. In order to cope with huge product flows, modern innovations should be used more extensively to manage these processes. Successful management will help provide quality service to rapidly changing business sectors. The Internet of Things (IoT) is a technology designed to process large amounts of data with maximum efficiency in real time. This technology can facilitate the implementation of smart identification, tracking, tracing, and management using radio frequency identification (RFID), infrared sensors, global positioning systems (GPS), laser scanners, and other detection tools. Such innovations as IoT have made a significant impact on warehousing operations. The aim of IoT is to perform administrative work, i.e., to efficiently manage warehouse data. IoT can be used to monitor and track goods, forecast demand trends, manage inventory, and perform other warehouse operations in real time. The key elements of a warehouse are sales and customer satisfaction. Implementing IoT improves financial performance, work productivity, and customer satisfaction. However, innovation requires additional investment in, for instance, implementation and maintenance. It is necessary to investigate how warehouse elements such as inventory accuracy or order processing time are affected by the internet of things in companies of different sizes. Research on the impact of IoT on warehouse management focuses on IoT advantages, disadvantages, mitigation risks, and the use of IoT in warehouses. The aim of this work is to research the impact of IoT on warehouse management in companies of different sizes and to determine whether the costs and benefits of IoT differ in the same scenario. As a result, the conceptual model for the adoption of IoT measures in warehouse companies was created, and its suitability was assessed by experts.