RESUMEN
BACKGROUND AND AIM: By using an in vivo phenotypic screening assay in zebrafish, we identified Convolamine, a tropane alkaloid from Convulvus plauricalis, as a positive modulator of the sigma-1 receptor (S1R). The wfs1abKO zebrafish larva, a model of Wolfram syndrome, exhibits an increased visual-motor response due to a mutation in Wolframin, a protein involved in endoplasmic reticulum-mitochondria communication. We previously reported that ligand activating S1R, restored the cellular and behavioral deficits in patient fibroblasts and zebrafish and mouse models. EXPERIMENTAL PROCEDURES: We screened a library of 108 repurposing and natural compounds on zebrafish motor response. KEY RESULTS: One hit, the tropane alkaloid Convolamine, restored normal mobility in wfs1abKO larvae without affecting wfs1abWT controls. They did not bind to the S1R agonist/antagonist binding site nor dissociated S1R from BiP, an S1R activity assay in vitro, but behaved as a positive modulator by shifting the IC50 value of the reference agonist PRE-084 to lower values. Convolamine restored learning in Wfs1∆Exon8 , Dizocilpine-treated, and Aß25-35 -treated mice. These effects were observed at low ~1 mg/kg doses, not shared by Convolvine, the desmethyl metabolite, and blocked by an S1R antagonist. CONCLUSION AND IMPLICATIONS: Convolamine therefore acts as an S1R positive modulator and this pharmacological action is relevant to the traditional use of Shankhpushpi in memory and cognitive protection.
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Alcaloides , Convolvulus , Receptores sigma , Humanos , Ratones , Animales , Receptor Sigma-1 , Receptores sigma/genética , Receptores sigma/metabolismo , Pez Cebra/metabolismo , Alcaloides/farmacología , CogniciónRESUMEN
Seven new hexasaccharide resin glycosides, named calysepins I-VII (1-7), with 27-membered rings, were obtained from the aerial parts of Calystegia sepium. Their structures with absolute configuration were established on the basis of spectroscopic data interpretation analysis and the use of chemical methods. They were defined as hexasaccharides composed of one d-quinovose, four d-glucose, and one l-rhamnose unit, and their sugar moieties were partially acylated by (2S)-methylbutanoic acid in 1-7 and (2R,3R)-nilic acid in 1-5 and 7, which mainly differed at the positions of acylation. Additionally, calysepin IV (4) exhibited cytotoxicity against A549 cells with an IC50 value of 5.2 µM.
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Antineoplásicos , Calystegia , Convolvulus , Calystegia/química , Glicósidos/química , Glicósidos/farmacología , Estructura Molecular , Resinas de Plantas/químicaRESUMEN
Convolvulus arvensis L. is an evergreen herb growing in various regions of Pakistan. Despite of several medicinal properties associated to this herb, it was not investigated scientifically for its bioactive compounds and detailed pharmaceutical properties. Therefore, its methanolic extract was divided into hexane (CA-H), chloroform (CA-C), ethyl acetate (CA-E) and butanol (CA-B) soluble fractions. CA-H and CA-C were found rich in phenolics (30.73±0.63 and 20.15±0.59â mg GAE/g of the extract, respectively), and the same fractions exhibited significant antioxidant activities (DPPH: 5.23±0.11 & 12.34±0.17â mg TE/g extract, respectively; ABTS: 36.82±0.04 & 56.74±0.61â mg TE/g extract, respectively). Also in CUPRAC activity assay, CA-H and CA-C exhibited highest activities as 87.30±0.46 and 56.74±0.61â mg TE/g extract, respectively, while CA-C was most active in FRAP activity assay with value of 40.21±2.19â mg TE/g extract. Total antioxidant capacity (1.23±0.033â mmol TE/g extract) was also found higher for CA-C, while CA-H activity was also comparable, however, CA-H showed higher metal chelating activity (22.74±0.001â mg EDTAE/g extract) than that of CA-C (17.55±0.22â mg EDTAE/g extract). These activities clearly revealed a direct relation between antioxidant potential and phenolic contents of CA-H and CA-C. In AChE and BChE inhibitory assay, CA-H and CA-E showed better inhibition (AChE: 8.24±0.77 & 4.46±0.007â mg GALAE/g extract; BChE: 5.40±0.02 & 1.92±0.24â mg GALAE/g extract) as compared to other fractions, whereas, against tyrosinase, CA-B was most active (37.35±0.53â mg KAE/g extract). CA-H and CA-C also showed higher inhibitory potential (0.98±0.08 & 0.58±0.01â mmol ACAE/g extract) against α-Amylase; while against α-Glucosidase, CA-E was the most active fraction. UHPLC/MS analysis of the methanolic extract of C. arvensis disclosed the presence of 62 compounds as sterols, triterpenes, flavonoids, fatty acids, alkaloids and coumarins. In Multivariate Analysis, the total phenolic contents were correlated strongly with all antioxidant assays except FRAP and DPPH. Regarding enzyme inhibitory properties, only AChE, BChE and α-amylase were correlated with the total phenolic contents in the extracts. Docking analyses confirmed these findings, as identified compounds had high binding free energy and inhibition constants with the enzymes studied. It was finally concluded that C. arvensis is a potential industrial crop, which can be a component of nutraceuticals and functional foods, if evaluated for its toxicity.
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Antioxidantes , Convolvulus , Antioxidantes/química , Extractos Vegetales/farmacología , Extractos Vegetales/química , Cromatografía Líquida de Alta Presión , alfa-Amilasas , Fenoles/química , Metanol/química , Análisis Multivariante , Industria Farmacéutica , Recursos Naturales , Fitoquímicos/farmacología , Fitoquímicos/análisisRESUMEN
This work aimed to develop and evaluate a post-acquisition data processing strategy, referred to as a mass defect filter (MDF), for rapid target the resin glycosides in root of Convolvulus scammonia by setting mass rang and mass defect range from high-resolution MS data. The full-scan mass data were acquired by high-performance liquid chromatography coupled with Q Exactive Plus hybrid quadrupole-orbitrap mass spectrometer that featured high resolution, mass accuracy, and sensitivity. To screen resin glycosides, three parent filter m/z 871, m/z 853, and m/z 869 combined with diagnostic fragment ions (DFIs) approach were applied to remove the interference from complex herbal extract. The targeted components were characterized based on detailed fragment ions. Using this approach, 80 targeted components, including 22 glycosidic acids and 58 resin glycosides were tentatively identified. The present results suggested that the proposed MDF strategy would be adaptable to the analysis of complex system in relevant filed.
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Convolvulus , Glicósidos , Cromatografía Líquida de Alta Presión/métodos , Glicósidos/química , Iones , Espectrometría de Masas/métodos , Resinas de PlantasRESUMEN
The antifungal drugs currently available and mostly used for the treatment of candidiasis exhibit the phenomena of toxicity and increasing resistance. In this context, plant materials might represent promising sources of antifungal agents. The aim of this study is to evaluate for the first time the chemical content of the volatile fractions (VFs) along with the antifungal and anti-biofilm of Convolvulus althaeoides L. roots. The chemical composition was determined by gas chromatography coupled to a flame ionization detector and mass spectrometry. In total, 73 and 86 chemical compounds were detected in the n-hexane (VF1) and chloroform (VF2) fractions, respectively. Analysis revealed the presence of four main compounds: n-hexadecenoic acid (29.77%), 4-vinyl guaiacol (12.2%), bis(2-ethylhexyl)-adipate (9.69%) and eicosane (3.98%) in the VF extracted by hexane (VF1). n-hexadecenoic acid (34.04%), benzyl alcohol (7.86%) and linoleic acid (7.30%) were the main compounds found in the VF extracted with chloroform (VF2). The antifungal minimum inhibitory concentrations (MICs) of the obtained fractions against Candida albicans, Candida glabrata and Candida tropicalis were determined by the micro-dilution technique and values against Candida spp. ranged from 0.87 to 3.5 mg/mL. The biofilm inhibitory concentrations (IBF) and sustained inhibition (BSI) assays on C. albicans, C. glabrata and C. tropicalis were also investigated. The VFs inhibited biofilm formation up to 0.87 mg/mL for C. albicans, up to 1.75 mg/mL against C. glabrata and up to 0.87 mg/mL against C. tropicalis. The obtained results highlighted the synergistic mechanism of the detected molecules in the prevention of candidosic biofilm formation.
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Antifúngicos , Convolvulus , Antifúngicos/farmacología , Antifúngicos/química , Hexanos , Cloroformo , Ácido Linoleico , Túnez , Cromatografía de Gases y Espectrometría de Masas , Biopelículas , Candida albicans , Candida tropicalis , Pruebas de Sensibilidad Microbiana , Candida glabrata , Adipatos , Guayacol , Alcoholes BencílicosRESUMEN
Convolvulus arvensis is used in Pakistani traditional medicine to treat inflammation-related disorders. Its anti-inflammatory potential was evaluated on hexane, dichloromethane, ethyl acetate, methanol, and aqueous extracts of whole plant on pro-inflammatory mediators in LPS-activated murine macrophage J774 cells at the non-cytotoxic concentration of 50 µg/mL. Ethyl acetate (ARE) and methanol (ARM) extracts significantly decreased mRNA levels of IL-6, TNF-α, MCP-1, COX-2, and iNOS. Furthermore, both extracts dose dependently decreased IL-6, TNF-α, and MCP-1 secretion. Forty-five compounds were putatively identified in ARE and ARM by dereplication (using HPLC-UV-HRMSn analysis and molecular networking), most of them are reported for the first time in C. arvensis, as for example, nineteen phenolic derivatives. Rutin, kaempferol-3-O-rutinoside, chlorogenic acid, 3,5-di-O-caffeoylquinic acid, N-trans-p-coumaroyl-tyramine, and N-trans-feruloyl-tyramine were main constituents identified and quantified by HPLC-PDA in ARE and ARM. Furthermore, chlorogenic acid, tyramine derivatives, and the mixture of the six identified major compounds significantly decreased IL-6 secretion by LPS-activated J774 cells. The activity of N-trans-p-coumaroyl-tyramine is shown here for the first time. Our results indicate that ARE, ARM and major constituents significantly inhibited the expression of pro-inflammatory mediators, which supports the use of this plant to treat inflammatory diseases.
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Antiinflamatorios/farmacología , Convolvulus/química , Inflamación/tratamiento farmacológico , Lipopolisacáridos/toxicidad , Macrófagos/efectos de los fármacos , Fitoquímicos/farmacología , Extractos Vegetales/farmacología , Animales , Inflamación/inducido químicamente , Macrófagos/inmunología , Ratones , Fitoquímicos/análisis , Extractos Vegetales/análisis , Hojas de la Planta/química , Células RAW 264.7RESUMEN
The aim of the present study was to quantify selected phenolic compounds, determine antioxidant activity and enzyme inhibitory effects of the aerial parts of Alkanna trichophylla Hub.-Mor. (A.â trichophylla) and Convolvulus galaticus Rost.ex Choisy (C.â galaticus) extracts prepared by homogenizer-assisted extraction (HAE), maceration (MAC) and infusion techniques. This is the first time such study has been designed to validate the phytochemical composition and bioactivity of these plants. Multivariate analysis was conducted on collected data. Rutin and caffeoylquinic acid derivatives were the most significant compounds in A.â trichophylla and C.â galaticus, respectively. The highest antioxidant activity of A.â trichophylla was mostly exhibited by HAE/methanolic extracts as determined by DPPH, ABTS, FRAP (51.39, 112.70 and 145.73â mg TE/g, respectively) and phosphomolybdenum (2.05â mmol TE/g) assays. However, significant antioxidant activities varied within the extracts of C.â galaticus. HAE/methanolic extract of A.â trichophylla significantly depressed AChE (2.70â mg GALAE/g), BChE (5.53â mg GALAE/g) and tyrosinase (26.34â mg KAE/g) activities and that of C.â galaticus inhibited AChE (2.04â mg GALAE/g), tyrosinase (31.25â mg KAE/g) and α-amylase (0.53â mmol ACAE/g) activities significantly. We concluded that HAE was the most efficient extraction technique as high yield of compounds and promising bioactivities were recorded from extracts prepared. Multivariate analysis showed that types of solvents influenced recovery of compounds and biological activities. This research study can be used as one methodological starting point for further investigation on these plants as all results are clearly promising and open the door to further research challenges such as cytotoxicity evaluation, molecular docking analysis, and more screening of pharmacological actions.
Asunto(s)
Antioxidantes/farmacología , Boraginaceae/química , Convolvulus/química , Inhibidores Enzimáticos/farmacología , Extractos Vegetales/farmacología , Plantas Medicinales/química , Acetilcolinesterasa/metabolismo , Agaricales/enzimología , Antioxidantes/química , Antioxidantes/aislamiento & purificación , Benzotiazoles/antagonistas & inhibidores , Compuestos de Bifenilo/antagonistas & inhibidores , Butirilcolinesterasa/metabolismo , Inhibidores Enzimáticos/química , Inhibidores Enzimáticos/aislamiento & purificación , Humanos , Monofenol Monooxigenasa/antagonistas & inhibidores , Monofenol Monooxigenasa/metabolismo , Picratos/antagonistas & inhibidores , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificación , Saccharomyces cerevisiae/enzimología , Ácidos Sulfónicos/antagonistas & inhibidores , Turquía , alfa-Amilasas/antagonistas & inhibidores , alfa-Amilasas/metabolismoRESUMEN
Convolvulus genus is a representative of the family of Convolvulaceae. Convolvulus plants are broadly distributed all over the world and has been used for many centuries as herbal medicine. Convolvulus genus contains various phytochemicals such as flavonoids, alkaloids, carbohydrates, phenolic compounds, mucilage, unsaturated sterols or terpenes, resin, tannins, lactones, and proteins. This review highlights the phytochemical composition, antimicrobial and antioxidant activities, application as food preservative, traditional medicine use, anticancer activities, and clinical effectiveness in human of Convolvulus plants. All the parts of Convolvulus plants possess therapeutic benefits; preliminary pharmacological data validated their use in traditional medicine. However, further preclinical and clinical experiments are warranted before any application in human health.
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Convolvulus/química , Fitoquímicos/farmacología , Extractos Vegetales/farmacología , Plantas Medicinales/química , Animales , Antiinfecciosos/farmacología , Antineoplásicos Fitogénicos/farmacología , Antioxidantes/farmacología , Convolvulus/clasificación , Conservantes de Alimentos , Humanos , Medicina Tradicional , Fitoterapia , Plantas Medicinales/clasificaciónRESUMEN
Six new glycosidic acids, arvensic acids E-J (1-6), were obtained from a glycosidic acid fraction afforded by alkaline hydrolysis of the crude resin glycosides from Convolvulus arvensis whole plants. Their structures were established from the spectroscopic data obtained and by chemical evidence. They were defined as heptasaccharides or hexasaccharides, comprising d-fucose, d-glucose, and l-rhamnose units. Compounds 1, 3, and 5 were assigned the 11 S-hydroxyheptadecanoic acid as the aglycone, while compounds 2, 4, and 6 were found to possess 11 S-hydroxyhexadecanoic acid as the aglycone. Compounds 1, 3, and 5 are the first representatives of resin glycosides with 11 S-hydroxyheptadecanoic acid as the aglycone.
Asunto(s)
Convolvulus/química , Glicósidos/química , Resinas de Plantas/química , Glicósidos/aislamiento & purificación , Hidrólisis , Espectroscopía de Resonancia Magnética , Estructura MolecularRESUMEN
A bacterial strain designated Tese-5T was isolated from a water convolvulus field in Taiwan and characterized using the polyphasic taxonomic approach. Strain Tese-5T was an aerobic, Gram-stain-negative, non-motile, rod-shaped bacterium and formed bright yellow coloured colonies. Strain Tese-5T grew at 15-35 °C (optimum, 30 °C), with 0-1.0â% NaCl (optimum, 0-0.5â%) and at pH 5.5-7 (optimum, pH 6). The major fatty acids (>10â%) of strain Tese-5T were C18â:â1ω7c, summed feature 3 (C16â:â1ω7c and/or C16â:â1ω6c) and C16â:â0. The polar lipid profile comprised phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine and sphingoglycolipid. The major polyamine was spermidine. The major isoprenoid quinone was Q-10. The DNA G+C content was 65.7 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Tese-5T belonged to the genus Novosphingobium and showed the highest levels of sequence similarity to Novosphingobium chloroacetimidivorans BUT-14T and Novosphingobium mathurense SM117T (96.3â%). Phenotypic characteristics of the novel strain also differed from those of the closest-related species of the genus Novosphingobium. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Tese-5T represents a novel species in the genus Novosphingobium, for which the name Novosphingobium ipomoeaesp. nov. is proposed. The type strain is Tese-5T (=BCRC 80904T=LMG 28838T=KCTC 42656T).
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Convolvulus/microbiología , Filogenia , Sphingomonadaceae/clasificación , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/genética , Ácidos Grasos/química , Hibridación de Ácido Nucleico , Fosfolípidos/química , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Espermidina/química , Sphingomonadaceae/genética , Sphingomonadaceae/aislamiento & purificación , Taiwán , Ubiquinona/químicaRESUMEN
AIMS: To identify a potential pathogenic isolate of fungus on Convolvulus arvensis and to determine its phytotoxic activity, which revealed the presence of toxic metabolites responsible for the toxicity against the target weed. METHODS AND RESULTS: A high virulent isolate of the fungus, Phoma crystallifera was isolated from symptomatic field bindweed in the west of Iran and was screened for the production of phytotoxins, which promoted necrosis on the detached leaves and seedlings of field bindweed in the bioassays. The isolate was distinct from other isolates of the fungi on the basis of morphological characteristics and the combined sequence database of the ITS region, partial LSU rDNA and ß-tubulin gene. Isolate P. crystalifera P6 produced the highest amount of phytotoxins after 21 days in a shacked culture of Richard's broth. The active metabolites were isolated from a cell-free culture filtrate by ethyl-acetate and purified by thin layer chromatography. The result indicated that six out of nine spots had phytotoxic activity in the bioassays, with Rf values of 0·16, 0·30, 0·36, 0·43, 0·57 and 0·81. CONCLUSIONS: Phoma crystallifera P6 and its active metabolites showed significant phytotoxic effects on the detached leaves of C. arvensis. SIGNIFICANCE AND IMPACT OF THE STUDY: To date, there are no reports of possible biocontrol agent(s) on C. arvensis in Iran. Thus, P. crystallifera P6 is introduced here as a severe pathogenic fungus and which can be used as a biocontrol agent against C. arvensis.
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Ascomicetos/aislamiento & purificación , Ascomicetos/metabolismo , Convolvulus/microbiología , Micotoxinas/metabolismo , Enfermedades de las Plantas/microbiología , Ascomicetos/genética , Ascomicetos/patogenicidad , Convolvulus/efectos de los fármacos , Irán , Micotoxinas/farmacología , Hojas de la Planta/microbiología , Malezas/efectos de los fármacos , Malezas/microbiología , Virulencia , Control de MalezasRESUMEN
OBJECTIVE: To isolate the neuroprotective component from Convolvulus pluricaulis Chois. (Convolvulaceae) which can be used as a lead molecule in the treatment of Huntington's disease (HD). METHODS: The methanolic extract of whole plant was fractionated into four fractions; chloroform, ethyl acetate, n-butanol, and aqueous fraction. The chloroform and ethyl acetate fractions were pooled on the basis of antioxidant activity and TLC profile and charged into silica gel column. Four subfractions were collected from column (FrA, FrB, FrC, and FrD) and further assessed for antioxidant potential by 2,2-diphenyl-1-picrylhydrazyl in vitro assay. 3-Nitropropionic acid (3-NP) was administered to Wister rats for induction of HD. Different fractions were administered for 14 days. Different behavioral alterations were assessed in between study period. Animals were euthanized immediately following the last behavioral session, and biochemical parameters were measured. RESULT AND DISCUSSION: Systemic administration of 3-NP showed marked motor deficits and oxidative damage in rats. Only FrB showed significant antioxidant activity and on further purification gave pure compound (scopoletin). Our study showed that FrB (20 mg/kg) pre-treatment significantly attenuated the loss in body weight, improved the locomotor activity, grip strength, and gait abnormalities. It also has attenuated the increased malondialdehyde and nitrite levels, and restored superoxide dismutase and reduced GSH enzyme activity in the striatum and cortex in 3-NP-treated groups. These results suggest that C. pluricaulis Chois. exhibits a neuroprotective effect by accelerating brain antioxidant defense mechanisms in 3-NP-treated rats.
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Antioxidantes/farmacología , Convolvulus/química , Enfermedad de Huntington/tratamiento farmacológico , Fármacos Neuroprotectores/farmacología , Animales , Conducta Animal , Peso Corporal , Cuerpo Estriado/efectos de los fármacos , Cuerpo Estriado/metabolismo , Glutatión/metabolismo , Enfermedad de Huntington/inducido químicamente , Peroxidación de Lípido/efectos de los fármacos , Masculino , Malondialdehído/metabolismo , Síndromes de Neurotoxicidad/tratamiento farmacológico , Nitritos/metabolismo , Nitrocompuestos/efectos adversos , Propionatos/efectos adversos , Ratas , Ratas Wistar , Superóxido Dismutasa/metabolismoRESUMEN
Oral administration of the total alcohol extract of Convolvulus pilosellifolius Desr. (250 and 500 md/kg) showed potent anti-ulcerogenic activity in absolute ethanol-induced ulcer model in rats; it showed percent protection of control ulcer by 69.2 and 84.6%, respectively, while standard ranitidine (100 mg/kg) exhibited 46.2%. Bio-guided work leads to isolation of two novel compounds (1 and 2), which were identified through 1 H, 13 C NMR, HMPC, HMQC and DEPT as: methyl 2-(hydroxymethyl) octanoate, named as amanitate, and 16-amino-9,13-dimethyl-17-(prop-1-en-2-yl)-hexadecahydro-1H-cyclopenta[a] phenanthren-3-ol, named as asmatol. Both compounds (50 mg/kg) possessed anti-ulcerogenic activity with 95.4% and 55.84% protection, respectively. Two known compounds (3 and 4) were also isolated and identified through comparison with authentic samples and confirmed through different NMR techniques as kampeferol and quercetin. These compounds also showed anti-ulcerogenic activity with 78.38% and 5.38% protection, respectively. The cytoprotective mechanism explains the potent anti-ulcerogenic activity of the total alcohol extract and the isolated compounds. The extract was highly safe as the LD50 was more than 5000 mg/kg. These results were well supported by the sub-chronic toxicity study, as the extract (500 mg/kg) administrated orally to rats for 35 consecutive days showed no alteration in the liver and kidney functions. Copyright © 2016 John Wiley & Sons, Ltd.
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Antiulcerosos/farmacología , Fraccionamiento Químico/métodos , Convolvulus/química , Extractos Vegetales/química , Úlcera Gástrica/tratamiento farmacológico , Animales , Masculino , Ratones , Ratas , Ratas WistarRESUMEN
In recent years, use of plants for remediation of contaminated soils, especially removal of heavy metals, is considered. The current study tends to investigate the removal of lead and nickel ions by the Convolvulus tricolor (CTC), was grown for 30 days in different concentrations of lead and nickel ions. Then concentration of them in soil and different organs of the plant was measured by atomic absorption spectrometry. The highest absorbed of them occurred in concentration 0.001N, which highest Pb(2+) accumulation is in the aerial parts of the plant: leaf > stem > root and for Ni(2)+: root > stem > leaf. No ion was observed into the flowers and nickel ion absorption is more of lead ion in different plant organs of CTC. The experimental isotherm data were investigated using isotherms of Langmuir, Freundlich, BET, Temkin and Dubinin-Radushkevich (DRK). The correlation coefficient for all of them is calculated that show the best correlation coefficient for Ni(2+) adsorption is obtained BET model, whereas for Pb(2+) adsorption in root is Freundlich model but for its leaf and stem is BET model. The results show, CTC is suitable for Pb(2+) and Ni(2+) and this technique is in-situ method, simple, and low cost.
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Convolvulus/metabolismo , Restauración y Remediación Ambiental/métodos , Metales Pesados/metabolismo , Contaminantes del Suelo/metabolismo , Adsorción , Biodegradación Ambiental , Convolvulus/química , Metales Pesados/análisis , Raíces de Plantas/química , Raíces de Plantas/metabolismo , Contaminantes del Suelo/análisisRESUMEN
BACKGROUND: Although switchgrass has been developed as a biofuel feedstock and its potential for bioethanol and bio-oil from fast pyrolysis reported in the literature, the use of the seeds of switchgrass as a source of triglycerides for biodiesel production has not been reported. Similarly, the potential for extracting triglycerides from coffeeweed (an invasive plant of no current economic value) needs to be investigated to ascertain its potential economic use for biodiesel production. RESULTS: The results show that coffeeweed and switchgrass seeds contain known triglycerides which are 983 and 1000 g kg(-1) respectively of the fatty acids found in edible vegetable oils such as sunflower, corn and soybean oils. In addition, the triglyceride yields of 53-67 g kg(-1) of the seed samples are in the range of commercial oil-producing seeds such as corn (42 g kg(-1) ). CONCLUSION: The results also indicate that the two non-edible oils could be used as substitutes for edible oil for biodiesel production. In addition, the use of seeds of switchgrass for non-edible oil production (as a feedstock for the production of biodiesel) further increases the total biofuel yield when switchgrass is cultivated for use as energy feedstock for pyrolysis oil and biodiesel production. © 2016 Society of Chemical Industry.
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Biocombustibles/análisis , Panicum/química , Aceites de Plantas/química , Malezas/química , Semillas/química , Sesbania/química , Triglicéridos/análisis , Cassia/química , Cassia/crecimiento & desarrollo , Convolvulus/química , Convolvulus/crecimiento & desarrollo , Cyperus/química , Cyperus/crecimiento & desarrollo , Ácidos Grasos/análisis , Ipomoea/química , Ipomoea/crecimiento & desarrollo , Panicum/crecimiento & desarrollo , Aceites de Plantas/aislamiento & purificación , Tubérculos de la Planta/química , Tubérculos de la Planta/crecimiento & desarrollo , Malezas/crecimiento & desarrollo , Semillas/crecimiento & desarrollo , Sesbania/crecimiento & desarrollo , Triglicéridos/química , Triglicéridos/aislamiento & purificación , Estados UnidosRESUMEN
Bois noir is an economically important grapevine yellows that is induced by 'Candidatus Phytoplasma solani' and principally vectored by the planthopper Hyalesthes obsoletus Signoret (Hemiptera: Cixiidae). This study explores the 'Ca. P. solani' genetic variability associated to the nettle-H. obsoletus and bindweed-H. obsoletus systems in vineyard agroecosystems of the central-eastern Italy. Molecular characterization of 'Ca. P. solani' isolates was carried out using polymerase chain reaction/restriction fragment length polymorphism to investigate the nonribosomal vmp1 gene. Seven phytoplasma vmp-types were detected among the host plants- and insect-associated field-collected samples. The vmp1 gene showed the highest polymorphism in the bindweed-H. obsoletus system, according to restriction fragment length polymorphism analysis, which is in agreement with nucleotide sequence analysis. Five vmp-types were associated with H. obsoletus from bindweed, of which one was solely restricted to planthoppers, with one genotype also in planthoppers from nettle. Type V12 was the most prevalent in both planthoppers and bindweed. H. obsoletus from nettle harbored three vmp-types, of which V3 was predominant. V3 was the only type detected for nettle. Our data demonstrate that planthoppers might have acquired some 'Ca. P. solani' profiles from other plant hosts before landing on nettle or bindweed. Overall, the different vmp1 gene rearrangements observed in these two plant hosts-H. obsoletus systems might represent different adaptations of the pathogen to the two host plants. Molecular information about the complex of vmp-types provides useful data for better understanding of Bois noir epidemiology in vineyard agroecosystem.
Asunto(s)
Proteínas Bacterianas/genética , Convolvulus/microbiología , Variación Genética , Hemípteros/microbiología , Phytoplasma/fisiología , Urtica dioica/microbiología , Animales , Italia , Datos de Secuencia Molecular , Filogenia , Phytoplasma/genética , Enfermedades de las Plantas/microbiología , Reacción en Cadena de la Polimerasa , Polimorfismo de Longitud del Fragmento de Restricción , Análisis de Secuencia de ADN , Vitis/microbiologíaRESUMEN
"Convolvulus hystrix Vahl" is a plant that has been known to Ancient Egyptians and to Arabs and its root was used traditionally as a purgative. Our attention was directed to the root bark as we observed that the largest part of the plant is deeply impeded underground. The work plan involved testing experimental animals for the influence of aqueous root bark extract on carbohydrate, fat and protein metabolisms as reflected on the growth and relevant laboratory metabolic assessment parameters. Proximate analysis showed high percentages of moisture (85%) and total lipids (7.2%) and surprising amounts of elements such as barium, strontium, rhodium and tellurium (1.7 mg, 3.1 mg, 8 mg and 9.1 mg/g ash, respectively). Random serum glucose value showed a significant decrease in the treated female group (p < 0.05). Serum total proteins of treated female group were found to be increased significantly (p < 0.001), while it was found to be decreased in the relevant treated male group (p < 0.01), together with a significant decrease in blood urea nitrogen (p < 0.05), with a significant increase in the serum creatinine (p < 0.05). Concerning serum fat metabolic parameters, a significant decrease in the serum triglycerides and high-density lipoproteins (p < 0.01 and p < 0.01, respectively) were found. We concluded that the presence of huge amount of polyphenolics such as tannins is responsible for the overall results documented as growth retarding and antinutritional factors. The results were motivating and pointed out to the possible opening of vast areas of research in the field of natural products. We recommend a series of biochemical and pharmacological studies concerning different parts of the plant as well.
Asunto(s)
Peso Corporal/efectos de los fármacos , Convolvulus/química , Extractos Vegetales/química , Extractos Vegetales/toxicidad , Animales , Ingestión de Alimentos/efectos de los fármacos , Femenino , Hígado/efectos de los fármacos , Hígado/patología , Masculino , Tamaño de los Órganos/efectos de los fármacos , Corteza de la Planta/química , Ratas , Ratas WistarRESUMEN
CONTEXT: Convolvulus pluricaulis Chois. (Convolvulaceae), a well-known Ayurvedic "Medhya Rasayana" (nervine tonic), is extensively used for different central nervous system (CNS) disorders. OBJECTIVE: The objective of this study was to evaluate the protective effect of standardized hydro-methanol extract of C. pluricaulis (CPE) and its fractions, namely ethyl acetate (EAE), butanol (BE), and aqueous (AE), against 3-nitropropionic acid (3-NP) induced neurotoxicity in rats. MATERIALS AND METHODS: The extract of the whole plant was standardized on the basis of scopoletin content (0.014%) using thin layer chromatography densitometric analysis. CPE (100 and 200 mg/kg) and its fractions, namely EAE (15 and 30 mg/kg), BE (25 and 50 mg/kg), and AE (50 and 100 mg/kg) were administered orally for 20 d. Their protective effect against 3-NP (10 mg/kg, i.p. for 14 d) was assessed by the effect on various behavioral parameters, namely body weight, locomotor activity, grip strength, gait pattern, and the effect on cognitive dysfunction. Biochemical parameters for oxidative damage were also assessed in the striatum and cortex regions of the brain. RESULTS: Administration of 3-NP induced HD-like symptoms that were indicated by reduced body weight, locomotor activity, memory, grip strength, and oxidative defense. CPE (200 mg/kg), EAE (30 mg/kg), and BE (50 mg/kg) significantly (p < 0.001) attenuated 3-NP induced reduction in locomotor activity, grip strength, memory, body weight, and oxidative defense in comparison with 3-NP-treated animals on 10 and 15 d. CONCLUSION: The present study suggested that CPE has a protective action against 3-NP-induced neurotoxicity and can be further explored for its efficacy against Huntington's disease.
Asunto(s)
Convolvulus , Fármacos Neuroprotectores/uso terapéutico , Síndromes de Neurotoxicidad/prevención & control , Nitrocompuestos/toxicidad , Extractos Vegetales/uso terapéutico , Propionatos/toxicidad , Animales , Masculino , Actividad Motora/efectos de los fármacos , Actividad Motora/fisiología , Fármacos Neuroprotectores/aislamiento & purificación , Fármacos Neuroprotectores/farmacología , Síndromes de Neurotoxicidad/metabolismo , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/farmacología , Ratas , Ratas WistarRESUMEN
5-Enolpyruvylshikimate-3-phosphate synthase (EPSPS), the target enzyme for glyphosate inhibition, catalyzes an essential step in the shikimate pathway for aromatic amino acid biosynthesis. The full-length cDNA of 1,751 nucleotides (CaEPSPS, Genbank accession number: EU698030) from Convolvulus arvensis was cloned and characterized. The CaEPSPS encodes a polypeptide of 520 amino acids with a calculated molecular weight of 55.5 kDa and an isoelectric point of 7.05. The results of homology analysis revealed that CaEPSPS showed highly homologous with EPSPS proteins from other plant species. Tissue expression pattern analysis indicated that CaEPSPS was constitutively expressed in stems, leaves and roots, with lower expression in roots. CaEPSPS expression level could increase significantly with glyphosate treatment, and reached its maximum at 24 h after glyphosate application. We fused CaEPSPS to the CaMV 35S promoter and introduced the chimeric gene into Arabidopsis. The resultant expression of CaEPSPS in transgenic Arabidopsis plants exhibited enhanced tolerance to glyphosate in comparison with control.
Asunto(s)
3-Fosfoshikimato 1-Carboxiviniltransferasa/genética , Clonación Molecular , Convolvulus/genética , Regulación de la Expresión Génica de las Plantas , 3-Fosfoshikimato 1-Carboxiviniltransferasa/metabolismo , Secuencia de Aminoácidos , Secuencia de Bases , Convolvulus/clasificación , Convolvulus/metabolismo , ADN Complementario/química , ADN Complementario/genética , Resistencia a Medicamentos/genética , Activación Enzimática , Perfilación de la Expresión Génica , Glicina/análogos & derivados , Glicina/farmacología , Herbicidas/farmacología , Datos de Secuencia Molecular , Fenotipo , Filogenia , Plantas Modificadas Genéticamente , Regiones Promotoras Genéticas , Alineación de Secuencia , GlifosatoRESUMEN
Urbanization alters the natural environment, with broad negative impacts on living organisms. Urbanization can also disrupt plant-pollinator networks by reducing the abundance and diversity of invertebrates. Firstly, I investigated whether the field bindweed (Convolvulus arvensis) is an obligatory entomophilous plant because previous reports were ambiguous. Secondly, I investigated how the obligatory entomophilous plant, field bindweed, responds to urbanization by comparing the flowering duration (anthesis) and the reproductive success of field bindweeds in urban and rural populations. Unlike cross-pollinated flowers and controls, flowers experimentally prevented from pollination and self-pollinated flowers did not produce seeds, suggesting that the field bindweed is self-incompatible and obligatory entomophilous. The abundance of urban pollinators was 5-6 times lower than the abundance of rural pollinators, and flies (Diptera), beetles (Coleoptera) and moths (Lepidoptera) were significantly more negatively influenced by the urban environment than hymenopterans (Hymenoptera). Urban plants showed significantly longer anthesis duration and lower reproductive success than rural plants. Illuminance and low pollinator abundance were negatively associated with the duration of the anthesis, but relative humidity did not affect the anthesis. Prolonged duration of the anthesis may be an adaptation to pollinator scarcity because more prolonged flowering increases the likelihood of pollination. Future research should unravel whether the longer anthesis of urban flowers is determined by behavioral plasticity or by the evolutionary selection of plants with a genetically determined longer anthesis.