UV irradiation induces apoptosis in the human endometrial stromal cell line (ThESC).

PURPOSE: The treatment options of endometrial hyperplasia consist of surgical, interventional and medical therapies including apoptosis-inducing agents. The purpose of the study was to evaluate the effects of ultraviolet (UV) radiation on the viability and the type of cell death on the human endometrial stromal cells (ThESC) line. METHODS: We investigated the effect of UV exposure on human endometrial stromal cell line (ThESC) on cell viability using MTT assay as well as changes in cell morphology using phase microscopy and acridine orange (AO)/ethidium bromide (EB) cell staining. RESULTS: UV treatment significantly decreased the percentage of the viable ThESC cells compared to the viability of untreated control cells using MTT assay (p<0.05). In addition, UV treatment of ThESC cells for 60 and 90 min induced high level of cell morphology disruption, followed with loss of both the cell shape and the presence of defragmented debris and stained with intense red color. CONCLUSIONS: The obtained results suggest the potential role of UV light application as additional treatment option of benign endometrium hyperplasia alone or in combination with other treatment modalities.

Radiotherapy in benign uterine bleeding disorders. The Radiumhemmet metropathia cohort 1912-1977. Short and long term results.

Radiotherapy was earlier a method of choice for treatment of benign bleeding disorders (metropathia), especially in women of high surgical risk. During the period 1912 to 1977 933 women with benign bleeding disorders were treated at Radiumhemmet with intracavitary brachytherapy or external irradiation or a combination of both. The result with regard to cure of the uterine bleedings was good (48%). Hormonal withdrawal symptoms after treatment were noted in 45% of the patients. In the long term follow up an increased risk of cardiovascular death was found in women treated before menopause. Malignant tumours occurred in 107 cases versus 90.2 expected (RR 1.19). The estimated ovarian dose of ionizing radiation varied from 3.5 Gy to 6.0 Gy for the three standard techniques. Two women gave birth to a healthy child 4 and 5 years after intracavitary radium treatment. The estimated absorbed dose to the ovaries in these two women were 1 Gy and 4 Gy, respectively.

[The correction of immunoendocrine disorders in patients with hyperplastic processes in the endometrium]. / Korrektsiia immunoéndokrinnykh narushenii u bol'nykh s giperplasticheskimi protsessami v éndometrii.

Effects were studied of extremely high-frequency electromagnetic radiation (EHF EMR) on indices for the immune and endocrine systems in a series of 48 patients presenting with hyperplastic processes in endometrium. EHF EMR-related normalization of values for the above systems was found out to take place together with a return of correlations back to normal, which fact makes it possible to use this modality as an immunocorrective factor.

[The correction of the immunohormonal disorders in patients with hyperplastic processes in the endometrium]. / Korrektsiia immunogormonal'nykh narushenii u bol'nykh s giperplasticheskimi protsessami v éndometrii.

Effects were studied of extremely high-frequency electromagnetic radiation (EHF ER) on indices for the immune and endocrine systems in 48 patients with hyperplastic processes in the endometrium. It is ascertain that along with the established normalization of parameters characterizing the above systems there occurs a return of correlations back to normal, which fact permits using EHF ER as an immunocorrective factor.