RESUMEN
Myxobacteria represent a viable source of chemically diverse and biologically active secondary metabolites. The myxochelins are a well-studied family of catecholate-type siderophores produced by various myxobacterial strains. Here, we report the discovery, isolation, and structure elucidation of three new myxochelins N1-N3 from the terrestrial myxobacterium Corallococcus sp. MCy9049, featuring an unusual nicotinic acid moiety. Precursor-directed biosynthesis (PDB) experiments and total synthesis were performed in order to confirm structures, improve access to pure compounds for bioactivity testing, and to devise a biosynthesis proposal. The combined evaluation of metabolome and genome data covering myxobacteria supports the notion that the new myxochelin congeners reported here are in fact frequent side products of the known myxochelin A biosynthetic pathway in myxobacteria.
Asunto(s)
Productos Biológicos/química , Lisina/análogos & derivados , Myxococcales/química , Niacina/química , Vías Biosintéticas/genética , Genoma Bacteriano/genética , Lisina/química , Metaboloma/genética , Myxococcales/genética , Myxococcales/aislamiento & purificación , Niacina/aislamiento & purificaciónAsunto(s)
Alcaloides/farmacología , Antineoplásicos Fitogénicos/farmacología , Medicamentos Herbarios Chinos/farmacología , Hidroxibutiratos/farmacología , Niacina/análogos & derivados , Tripterygium/química , Alcaloides/química , Alcaloides/aislamiento & purificación , Animales , Antiinflamatorios , Antineoplásicos Fitogénicos/química , Antineoplásicos Fitogénicos/aislamiento & purificación , Proliferación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Ensayos de Selección de Medicamentos Antitumorales , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/aislamiento & purificación , Hidroxibutiratos/química , Hidroxibutiratos/aislamiento & purificación , Espectroscopía de Resonancia Magnética , Ratones , Conformación Molecular , Niacina/química , Niacina/aislamiento & purificación , Niacina/farmacología , Células RAW 264.7RESUMEN
OBJECTIVE: To investigate the secondary metabolites from Penicillium raistrickii. METHODS: Compounds were isolated and purified by normal and reverse phase silica gel, Sephadex LH-20 gel column chromatography and RP-HPLC. Their structures were established by means of spectral techniques and physicochemical properties. RESULTS: Twelve compounds were identified as pestafolide A(II), 3-methoxy-4-methyl-2,4-dien-pentanoic acid (2),p-hydroxy phenylacetamide (3),2-(2-hydroxy propanamido) benzamide (4), nicotinic acid (5), thymine (6), uracil (7) cyclo (Gly-Ala) (8), (22E,24R)-3ß,5α,9α-trihydroxy ergosta-7,22-diene-6-one (9), cerevisterol (10), ergosterol (11) and ergosterol peroxide (12). CONCLUSION: All compounds are isolated from Penicillium raistrickii for the first time.
Asunto(s)
Penicillium/química , Benzamidas/química , Benzamidas/aislamiento & purificación , Dipéptidos/química , Dipéptidos/aislamiento & purificación , Ergosterol/análogos & derivados , Ergosterol/química , Ergosterol/aislamiento & purificación , Niacina/química , Niacina/aislamiento & purificación , Fitosteroles/química , Fitosteroles/aislamiento & purificación , Metabolismo SecundarioRESUMEN
The structure of biomembranes was imitated by introducing nonionic surfactant polyoxyethylene (23) dodecylether, cholic acid and endogenic thermostable protein complex (14-65 kDa) into the mobile phase. The influence of concentration of these additives on the retention of the model compounds was studied. The competing interaction of cholic acid and endogenic thermostable protein complex in the lipid bilayer model was revealed on the basis of chromatographic data. The values of efficiency of the chromatographic column regarding solutes were increased by addition of endogenic thermostable protein complex to the mobile phase containing Brij-35 and cholic acid.
Asunto(s)
Ácido Cólico/química , Cromatografía/métodos , Membrana Dobles de Lípidos/química , Proteínas de la Membrana/química , Micelas , Modelos Químicos , Animales , Modelos Biológicos , Niacina/aislamiento & purificación , Polietilenglicoles/química , Ratas , Sacarina/aislamiento & purificación , Tensoactivos/químicaRESUMEN
OBJECTIVE: To study the chemical constituents of endophytic fungus Fimetariella rabenhorsti isolated from Aquilaria sinensis. METHODS: Chemical constituents of the fungus were isolated and purified by column chromatography and their structures were elucidated on the basis of spectral data. RESULTS: Five compounds were isolated and identified as 4-hydroxy-phenylethyl alcohol (1),nicotinic acid (2), D-galacitol(3), 2-anilino-1,4-naphthoquinone (4), N-phenylacetamide (5). CONCLUSION: Compounds 1-5 are isolated from the genus Fimetariella for the first time, compound 5 is rare in natural products.
Asunto(s)
Ascomicetos/química , Niacina/aislamiento & purificación , Alcohol Feniletílico/análogos & derivados , Thymelaeaceae/microbiología , Acetanilidas/química , Acetanilidas/aislamiento & purificación , Ascomicetos/aislamiento & purificación , Fermentación , Espectroscopía de Resonancia Magnética , Estructura Molecular , Niacina/química , Alcohol Feniletílico/química , Alcohol Feniletílico/aislamiento & purificaciónRESUMEN
We demonstrate that the combination of high speed separations on chip with a fast mass spectrometer enables electrophoretic separations with full mass spectra registration within a second. This was accomplished by coupling a microfluidic glass chip with an integrated nanospray emitter to a fast time of flight mass spectrometer working at 100 Hz for data acquisition. Applying field strengths up to 5800 V cm(-1) we achieved separations of model analytes such as pharmaceuticals and peptides with subsequent acquisition of full mass spectra within one second or slightly more.
Asunto(s)
Electroforesis/instrumentación , Espectrometría de Masas/instrumentación , Arginina/análisis , Arginina/aislamiento & purificación , Electroforesis/métodos , Espectrometría de Masas/métodos , Técnicas Analíticas Microfluídicas , Niacina/análisis , Niacina/aislamiento & purificación , Espermidina/análisis , Espermidina/aislamiento & purificación , Factores de TiempoRESUMEN
OBJECTIVE: To study the chemical constituents from aerial parts of Gynura divaricata. METHODS: The constituents were isolated on silica gel column chromatography, preparative TLC and Sephadex LH-20 column chromatography, identified by physicochemical properties and the structures were elucidated by spectral analysis. RESULTS: 10 compounds were isolated and identified as 2-(1', 2', 3', 4'-tetrahydroxybutyl)-6-(2", 3", 4"-trihydroxybutyl)-pyrazine (1), 2-(1', 2', 3', 4'-tetrahydroxybutyl)-5-(2", 3", 4"-trihydroxybutyl) -pyrazine (2), nicotinic acid (3), 5-hydroxy-picolinic acid(4), methyl-5-hydroxy-2- pyridinecarboxylate (5), adenosine (6), uridine (7), stigmasterol-5-O- beta-D-glucoside (8), dibutyl terephthalate (9), methyl chlorogenate (10). CONCLUSION: Compounds 1, 2, 5, 9, 10 are obtained from this genus for the first time, Compounds 3, 4 are obtained from this plant for the first time.
Asunto(s)
Asteraceae/química , Niacina/aislamiento & purificación , Ácidos Picolínicos/aislamiento & purificación , Componentes Aéreos de las Plantas/química , Plantas Medicinales/química , Adenosina/química , Adenosina/aislamiento & purificación , Ácido Clorogénico/análogos & derivados , Ácido Clorogénico/química , Ácido Clorogénico/aislamiento & purificación , Espectroscopía de Resonancia Magnética , Estructura Molecular , Niacina/química , Ácidos Picolínicos/química , Uridina/química , Uridina/aislamiento & purificaciónRESUMEN
OBJECTIVE: In an effort to investigate the chemical constitutes of submerged cultivation mycelium of Cordyceps jiangxiensis anamorph. METHODS: The mycelia of C. jiangxiensis anamorph produced by submerged cultivation technique were separated and purified using various chromatographic techniques such as repeated liquid column chromatography, preparative thin layer chromatography etc. , and then the chemical structures of compounds obtained were elucidated on the basis of physicochemical analysis and spectroscopic techniques such as 1H-NMR, 13C-NMR and MS. RESULTS: Nine compounds were obtained from the petroleum ether and ethyl acetate extractive fractions of mycelial methanol extract, and were identified as follows: uracil (I), adenine (II), adenosine (III), uridine (IV), 3'-methoxyuridine (V), succinic acid (VI), nicotinic acid (VII), 1-monostearin (VIII), tetracosanoic acid (IX). CONCLUSION: Chemical constitutes of C. jiangxiensis anamorph are systematically separated for the first time, and compounds V, VII, VIII, and IX obtained are separated from Cordyceps species firstly.
Asunto(s)
Cordyceps/química , Micelio/química , Niacina/aislamiento & purificación , Nucleósidos/aislamiento & purificación , Ácido Succínico/aislamiento & purificación , Cromatografía Liquida , Ácidos Grasos/química , Ácidos Grasos/aislamiento & purificación , Estructura Molecular , Niacina/química , Nucleósidos/química , Ácido Succínico/químicaRESUMEN
Poly(N-isopropylacrylamide) is a temperature-responsive polymer, which is hydrophilic at low temperature but hydrophobic at high temperature. Using this characteristic, the polymer was bonded to the inner surface of fused-silica capillary to prepare a temperature-responsive column to study the separation behavior in electrochromatography.
Asunto(s)
Acrilamidas/química , Electrocromatografía Capilar/métodos , Polímeros/química , Resinas Acrílicas , Ácido Benzoico/química , Ácido Benzoico/aislamiento & purificación , Interacciones Hidrofóbicas e Hidrofílicas , Niacina/química , Niacina/aislamiento & purificación , Dióxido de Silicio/química , TemperaturaRESUMEN
Six compounds have been isolated from the leaves of Pyrenacantha staudtii, two of which are new compounds. The new compounds have been characterized as kaempherol 3-O-beta-rhamnopyranosyl (1-->6)-beta-D-glucopyranoside (1) and 4-beta-glucopyranosyl-(2-furyl)-5-methy-1,2-glucopyranoside phenylmethanone (2). The known compounds are 3-pyridinecarboxylic acid (3), beta-sitosterol (4), sitosterol 3-O-beta-glucopyranoside (5) and taraxerol (6). Their structures were determined by spectroscopic and chemical evidences. The two new compounds together with 3-pyridinecarboxylic acid showed significant in vitro xanthine oxidase inhibitory activity. To the best of our knowledge, this is the first report of these compounds from this plant.
Asunto(s)
Inhibidores Enzimáticos/aislamiento & purificación , Glucósidos/aislamiento & purificación , Quempferoles/aislamiento & purificación , Magnoliopsida/química , Xantina Oxidasa/metabolismo , Inhibidores Enzimáticos/química , Inhibidores Enzimáticos/farmacología , Glucósidos/química , Glucósidos/farmacología , Quempferoles/química , Quempferoles/farmacología , Estructura Molecular , Niacina/química , Niacina/aislamiento & purificación , Niacina/farmacología , Hojas de la Planta/química , Plantas Medicinales/químicaRESUMEN
A chitosan membrane composed by 60% (w/w) chitosan and 40% (w/w) Aliquat®336 has been proposed as a new biopolymeric support for electromembrane extraction. The new support has been characterized by Scanning Electron Microscopy, resulting a 30-35⯵m thickness. Amoxicillin, nicotinic acid, hippuric acid, salicylic acid, anthranilic acid, ketoprofen, naproxen and ibuprofen have been successfully extracted using the proposed support. Better enrichment factors were obtained for the acidic polar analytes than for the non-steroidal anti-inflammatory compounds (ranging from 118 for hippuric acid and 20 for ibuprofen). Electromembrane extraction was developed applying a DC voltage of 100â¯V, 1-octanol as supported liquid membrane and 20â¯min of extraction. The target analytes have also been satisfactorily extracted from human urine samples, providing high extraction efficiencies. The chitosan membrane is presented as a promising alternative for supporting liquid membrane compared to commonly used materials for this purpose.
Asunto(s)
Biopolímeros/química , Quitosano , Técnicas Electroquímicas , Amoxicilina/química , Amoxicilina/aislamiento & purificación , Hipuratos/química , Hipuratos/aislamiento & purificación , Humanos , Ibuprofeno/química , Ibuprofeno/aislamiento & purificación , Cetoprofeno/química , Cetoprofeno/aislamiento & purificación , Naproxeno/química , Naproxeno/aislamiento & purificación , Niacina/química , Niacina/aislamiento & purificación , Ácido Salicílico/química , Ácido Salicílico/aislamiento & purificación , ortoaminobenzoatos/química , ortoaminobenzoatos/aislamiento & purificaciónRESUMEN
The use of polymer inclusion membranes (PIMs) as support of 1-octanol liquid membrane in electromembrane extraction (EME) procedure is proposed. Synthesis of PIMs were optimized to a composition of 29% (w/w) of cellulose triacetate as base polymer and 71% (w/w) of Aliquat®336 as cationic carrier. Flat PIMs of 25µm thickness and 6mm diameter were used. EME protocol was implemented for the simultaneous extraction of four non-steroidal anti-inflammatory drugs (NSAIDs) (salicylic acid, ketoprofen, naproxen and ibuprofen) and four highly polar acidic drugs (anthranilic acid, nicotinic acid, amoxicillin and hippuric acid). Posterior HPLC separation of the extracted analytes was developed with diode array detection. Recoveries in the 81-34% range were obtained. EME procedure was applied to human urine samples.
Asunto(s)
Antiinflamatorios no Esteroideos/aislamiento & purificación , Celulosa/análogos & derivados , Técnicas Electroquímicas , Compuestos de Amonio Cuaternario/química , Amoxicilina/aislamiento & purificación , Amoxicilina/orina , Antiinflamatorios no Esteroideos/orina , Celulosa/química , Hipuratos/aislamiento & purificación , Hipuratos/orina , Ibuprofeno/aislamiento & purificación , Ibuprofeno/orina , Cetoprofeno/aislamiento & purificación , Cetoprofeno/orina , Membranas Artificiales , Naproxeno/aislamiento & purificación , Naproxeno/orina , Niacina/aislamiento & purificación , Niacina/orina , Ácido Salicílico/aislamiento & purificación , Ácido Salicílico/orina , ortoaminobenzoatos/aislamiento & purificación , ortoaminobenzoatos/orinaRESUMEN
A new support has been proposed to be used for carrier-mediated electromembrane extraction purposes. The new support (Tiss®-OH) is a 100µm thickness sheet nanofiber membrane manufactured by electrospinning and composed by acrylic nanofibers. It has been used in an electromembrane extraction (EME) combined with a HPLC procedure using diode array detection. The proposed method has been used for the extraction of four high polarity acidic compounds: nicotinic acid, amoxicillin, hippuric acid and salicylic acid. Analytes were extracted from an aqueous sample solution (pH 4) (donor phase) using a Tiss®-OH sheet that supports a 5% (w/v) Aliquat®336 in 1-octanol liquid membrane. Aqueous solution (pH 6) was used as acceptor phase. The electrical field was generated from a d.c. electrical current of 100V through two spiral shaped platinum wires placed into donor and acceptor phases. Analytes were extracted in 10min with recoveries in the 60-85% range. The proposed EME procedure has been successfully applied to the determination of the target analytes in human urine samples.
Asunto(s)
Técnicas Electroquímicas/métodos , Membranas Artificiales , Nanoestructuras/química , Compuestos Orgánicos/análisis , Amoxicilina/análisis , Amoxicilina/aislamiento & purificación , Amoxicilina/orina , Cromatografía Líquida de Alta Presión , Hipuratos/análisis , Hipuratos/aislamiento & purificación , Humanos , Concentración de Iones de Hidrógeno , Nanoestructuras/ultraestructura , Niacina/análisis , Niacina/aislamiento & purificación , Compuestos Orgánicos/aislamiento & purificación , Reproducibilidad de los Resultados , Ácido Salicílico/análisis , Ácido Salicílico/aislamiento & purificaciónRESUMEN
Temperature affects not only the chromatographic characteristics of solute but may also alter the conformation of the stationary phase. However, temperature influences on enantioseparation of solute and conformation of chiral stationary phase (CSP) are seldom considered simultaneously. In this study, three temperature programs, a conventional heating procedure, a cyclic van't Hoff program, and a step-temperature program, were employed to evaluate temperature effects on enantioseparation of five imidazolinone herbicides on Chiralcel OJ column and the conformational state of the stationary phase. The van't Hoff plots of retention factor (k'), distribution constant (K) and separation factor (alpha) for imazapyr (1), imazapic (2), imazethapyr (3), and imazamox (4) were linear within 15-50 degrees C. Nonlinear van't Hoff plots of alpha were observed for imazaquin (5) with mobile phase of n-hexane (0.1% TFA)-2-propanol at 70/30 or 60/40 (v/v). The large molecular size of imazaquin (5) and van't Hoff plots of alpha were therefore more sensitive at detecting conformational changes of the stationary phase. Small but irreversible conformational changes occurred at 5-10 degrees C with the solvent ratio of 60/40. During the cyclic van't Hoff program, reversible conformational changes were observed at >or=15 degrees C. A switch was even visible at about 25 degrees C with the solvent ratio of 60/40 during the re-cooling cycle. The cyclic van't Hoff temperature program showed that using OJ column may yield satisfactory results at 15-50 degrees C but not at Asunto(s)
Cromatografía Líquida de Alta Presión/métodos
, Herbicidas/aislamiento & purificación
, Imidazoles/aislamiento & purificación
, Temperatura
, Cromatografía Líquida de Alta Presión/instrumentación
, Herbicidas/química
, Imidazoles/química
, Estructura Molecular
, Niacina/análogos & derivados
, Niacina/química
, Niacina/aislamiento & purificación
, Quinolinas/química
, Quinolinas/aislamiento & purificación
, Reproducibilidad de los Resultados
, Solventes/química
, Estereoisomerismo
RESUMEN
The growing interest in application of ionic liquids (ILs) in analytical chemistry has been observed. The aim of presented investigation was to verify whether ILs would be a suitable modifier of the background electrolyte (BGE) for pharmaceutical analysis of the closely related drug analogues. The study demonstrates the use of 1-ethyl-3-methylimidazolium tetrafluoroborate (1E-3MI-TFB) ionic liquid as modifiers in the separation of nicotinic acid and its structural isomers by capillary electrophoresis. Dependences of the ionic liquid concentration in a BGE on the separation parameters like migration time, resolution factor and width at peak's baseline have been compared. The separation mechanism involves the free imidazolium ions, which can interact with inner surface of the capillary wall. Increased 1E-3MI-TFB concentration to 150 mmol/L caused decrease of migration times of analytes, improve peaks shape and increase of separation performances. At this ionic liquid concentration in a BGE resolution factor between nicotinic and isonicotinic acids increased to 1.86. The proposed CE separation procedure is highly reproducible and can be applied in qualitative and quantitative analysis of carboxylic acids.
Asunto(s)
Química Farmacéutica/métodos , Electroforesis Capilar/métodos , Imidazoles/química , Niacina/aislamiento & purificación , Tecnología Farmacéutica/métodos , Tampones (Química) , Ácidos Carboxílicos/química , Cromatografía Líquida de Alta Presión/métodos , Relación Dosis-Respuesta a Droga , Iones , Ácidos Isonicotínicos/química , Modelos Químicos , Niacina/análisis , Niacina/química , Ácidos Picolínicos/química , Estereoisomerismo , Factores de TiempoRESUMEN
AIM: To study the chemical constituents of Sphaerophysa salsula (Pall.) DC. METHODS: Compounds were isolated by repeated chromatography, on silica gel and Sephadex LH-20 columns and identified by spectroscopic data (ESI-MS, 1H and 13C NMR, HSQC, HMBC). RESULTS: Eight compounds have been isolated and identified as: isorhamnetin-3-O-[6"-(3-hydroxy-3-methylglutaroyl)-beta-D-glucopyranoside] (I), quercitrin (II), n-butyl-O-beta-D-fructopyranoside (III), nicotinic acid (IV), succinic acid (V), erythrol (VI), D-mannitol (VII), uridine (VIII). CONCLUSION: Compound I is a new compound. Compounds I -VI were isolated from this genus for the first time.
Asunto(s)
Fabaceae/química , Flavonoles/aislamiento & purificación , Glucósidos/aislamiento & purificación , Plantas Medicinales/química , Flavonoles/química , Glucósidos/química , Conformación Molecular , Estructura Molecular , Niacina/química , Niacina/aislamiento & purificación , Raíces de Plantas/química , Tallos de la Planta/química , Quercetina/análogos & derivados , Quercetina/química , Quercetina/aislamiento & purificaciónRESUMEN
We propose an original model of effective diffusion along packed beds of mesoporous particles for HILIC developed by combining Torquatos model for heterogeneous beds (external eluent+particles), Landauers model for porous particles (solid skeleton+internal eluent), and the time-averaged model for the internal eluent (bulk phase+diffuse water (W) layer+rigid W layer). The new model allows to determine the analyte concentration in rigid and diffuse W layer from the experimentally determined retention factor and intra-particle diffusivity and thus to distinguish the retentive contributions from adsorption and partitioning. We apply the model to investigate the separation of toluene (TO, as a non-retained compound), nortriptyline (NT), cytosine (CYT), and niacin (NA) on an organic ethyl/inorganic silica hybrid adsorbent. Elution conditions are varied through the choice of a third solvent (W, ethanol, tetrahydrofuran (THF), acetonitrile (ACN), or n-hexane) in a mobile phase (MP) of ACN/aqueous acetate buffer (pH 5)/third solvent (90/5/5, v/v/v). Whereas NA and CYT retention factors increase monotonously from W to n-hexane as third solvent, NT retention reaches its maximum with polar aprotic third solvents. The involved equilibrium constants for adsorption and partitioning, however, do not follow the same trends as the overall retention factors. NT retention is dominated by partitioning and NA retention by adsorption, while CYT retention is controlled by adsorption rather than partitioning. Our results reveal that the relative importance of adsorption and partitioning mechanisms depends in a complex way from analyte properties and experimental parameters and cannot be predicted generally.
Asunto(s)
Cromatografía Liquida/métodos , Adsorción , Tampones (Química) , Citosina/aislamiento & purificación , Difusión , Interacciones Hidrofóbicas e Hidrofílicas , Niacina/aislamiento & purificación , Nortriptilina/aislamiento & purificación , Porosidad , Dióxido de Silicio , Solventes , Tolueno/aislamiento & purificaciónRESUMEN
The present study was designed to isolate and evaluate the antibacterial activity of the compounds from the whole plant of Euphorbia helioscopia L.. Various chromatographic techniques were used to isolate and purify the compound. The structure of the compound was elucidated on basis of spectral data ((1)H NMR, (13)C NMR, (1)H-(1)H COSY, HSQC, HMBC, NOESY, IR, and HR-ESI-MS). A new jatrophone-type diterpenoid (14α,15ß-diacetoxy-3ß-benzoyloxy-7ß-nicotinoyloxy-9-oxo-jatropha-5E,11E-diene), named euphoheliosnoid E (1), was isolated from the whole plant of E. helioscopia L. Compound 1 showed significant anti-microbial activity against oral pathogens.
Asunto(s)
Diterpenos/aislamiento & purificación , Euphorbia/química , Niacina/análogos & derivados , Extractos Vegetales/química , Antiinfecciosos/aislamiento & purificación , Antiinfecciosos/farmacología , Diterpenos/química , Diterpenos/farmacología , Estructura Molecular , Enfermedades de la Boca/microbiología , Niacina/química , Niacina/aislamiento & purificación , Niacina/farmacología , Extractos Vegetales/farmacologíaRESUMEN
A standard sample and method for performance evaluation in capillary zone electrophoresis is defined. The sample, containing thiamine, nicotinamide and nicotinic acid, was analysed in a 20 mM sodium phosphate buffer pH 7. The impact of pH, buffer type and ionic strength on electroosmotic flow, electrophoretic mobility, and peak shape was investigated. The separation of several water-soluble vitamins in phosphate buffer pH 7 was developed in order to analyse an over-the-counter vitamin preparation. Spectral analysis and peak purity tests were applied on-line for peak identification.
Asunto(s)
Electroforesis/métodos , Vitaminas/aislamiento & purificación , Estudios de Evaluación como Asunto , Concentración de Iones de Hidrógeno , Niacina/aislamiento & purificación , Niacinamida/aislamiento & purificación , Reproducibilidad de los Resultados , Solubilidad , Análisis Espectral , Tiamina/aislamiento & purificaciónRESUMEN
Supercritical fluid chromatography (SFC) has become a technique for solving problems that are difficult to be monitored by other chromatographic methods. However, the most widely used fluid, is no more polar than hexane. Polar samples which are difficult to be analyzed with pure supercritical CO(2) because of their high polarity can be separated by adding polar modifiers to supercritical CO(2). In this paper various vitamins were well separated using water-modified supercritical CO(2) fluid. The amount of water dissolved in supercritical CO(2) was measured using an amperometric microsensor made of a thin film of perfluorosulfonate ionomer (PFSI).