Characterization of silk sutures coated with propolis and biogenic silver nanoparticles (AgNPs); an eco-friendly solution with wound healing potential against surgical site infections (SSIs)

Background/aim: Bacterial adherence to a suture material is one of the main causes of surgical site infections. An antibacterial suture material with enhanced wound healing function may protect the surgical site from infections. Thus, the present study aimed to investigate the synergistic effect of propolis and biogenic metallic nanoparticles when combined with silk sutures for biomedical use. Materials and methods: Silver nanoparticle (AgNP) synthesis was carried out via a microbial-mediated biological route and impregnated on propolis-loaded silk sutures using an in situ process. Silk sutures fabricated with propolis and biosynthesized AgNPs (bioAgNP-propolis-coated sutures) were intensively characterized using scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). The antibacterial characteristics of the bioAgNP-propolis-coated sutures were evaluated using the agar plate method. The biocompatibility of the bioAgNP-propolis- coated sutures was evaluated using 3T3 fibroblast cells, and their wound-healing potential was also investigated. Results: BioAgNP-propolis-coated sutures displayed potent antibacterial activity against pathogenic gram-negative and gram-positive bacteria, Escherichia coli and Staphylococcus aureus, respectively. BioAgNP-propolis-coated silk sutures were found to be biocompatible with 3T3 fibroblast cell culture. In vitro wound healing scratch assay also demonstrated that the extract of bioAgNP-propolis-coated sutures stimulated the 3T3 fibroblasts' cell proliferation. Conclusion: Coating the silk sutures with propolis and biogenic AgNPs gave an effective antibacterial capacity to surgical sutures besides providing biocompatibility and wound healing activity.

In vitro and in vivo study of naturally derived alginate/hydroxyapatite bio composite scaffolds.

Biogenic bioceramics scaffolds are receiving considerable attention for bone restoration applications. Compared with scaffolds of chemical origin, biogenic scaffolds exhibit greater biocompatibility and enhanced bioactive features. In the present study, porous biogenic hydroxyapatite (bHA) was prepared via a polymeric infiltration route and was subsequently coated with alginate to produce alginate/biogenic hydroxyapatite (Alg/bHA) composites. Alginate was used to enhance the mechanical properties as well as the bioactivity and biodegradability of the HA scaffolds. A coating of 3%w/v alginate applied for 10 min was found to result in the best coating for the HA porous scaffolds. The in vitro study demonstrated that the prepared composites had acceptable bioactivity and biodegradability characteristics. The histological study in femur bone of rats indicated that the 3Alg/HA scaffolds capable of supporting both endochondral and intramembranous bone formation. The defect was fully regenerated and mostly filled with the mature lamellar bone after 6 months, with Ca/P atomic ratio similar to the rat's normal bone. The studied scaffolds provide a promising therapeutic option to enhance local bone healing because they do not damage liver or kidney functions and do not induce carcinogenic or inflammatory effects. Accordingly, 3Alg/HA scaffolds are recommended for the tissue engineering applications.

[2 cases of death following cell therapy]. / Zwei Todesfälle nach Zelltherapie.

Fresh-cell therapy is a paramedical procedure whose claimed therapeutic success has not been proven by customary clinical tests (randomized, double-blind trials). In addition, the qualitatively and quantitatively non-standardized parenteral application of heterologous antigens presents considerable danger for the recipient in the form of fatal immune reactions. Two cases are reported in which history, clinical findings and autopsy provided evidence of a causal relationship between cell therapy and death. In one instance, a 75-year-old woman died 30 days after an intramuscular injection of quick-frozen fresh cells from the effects of an immune-complex vasculitis; in the other, a 60-year-old woman died 14 days after "original fresh-cell treatment after Prof. Niehans" from perivenous leucoencephalitis.

[Primary screening of the immunopharmacologic activity of Filatov tissue therapy preparations]. / Pervichnyi skrining immunofarmakologicheskoi aktivnosti preparatov tkanevoi terapii po V.P. Filatovu.

The paper presents results of primary assessment of immunopharmacologic activity of tissue preparations (peloidodistillate, FiBS, torfot, extract of aloe, placental extract) and the study of their influence on basic links of immune response (experimental investigations were carried out with mice-hybrids F1). It is found that the studied bioregulators possess immunomodulating action on primary humoral immune response and don't increase a hypersensitive reaction of a slowed type. The direction of immunotropic action of tissue preparations depends on the state of immunologic reactivity of the body and the kind of a preparation (variety of immunopharmacologic activity of the distillates groups and albumin preparations). It is expedient to work out indications to the usage of tissue preparations after V. P. Filatov to prevent and treat immunodeficient and immunopathologic conditions of the body.

[Effect of Filatov's tissue preparation and and a globulin preparation on weight gain in calves]. / Vliianie na tukanen preparat po Filatov i na globulinovi preparati vurkhu prirasta na teletata

A comparitive experiment was carried out with 81 calves, aged 4-15 days, to establish the effect of a tissue preparation of swine embryos after Filatov, colostral lactoplasmin, and bovine gammaglobulin as prophylactic and therapeutic means, on the sera proteins. It was found that these preparations show no influence on the protein fraction ratio. It has also been pointed out that these agents produce a strong stimulative effect on the average daily weight gain of calves up to the sixtieth day following treatment.

[Effect of lactoplasmin, gamma-globulin, Filatov's swine embryo tissue preparation and hyperimmune rabbit serum on several hematologic indices and morbidity among cattle with bronchopneumonia]. / Vliianie na laktoplazmin, gamma-globulin tukanen preparat ot svinski embrioni po Filatov i khiperimunen zaeshki serum burkhu niakoi khematologichni pokazateli i zaboliaemostta na teletata ot bronkhopnevmoniia

Studied was the effect of lactoplasmin, gamma-globulin, a tissue preparation of swine embryos after Filatov, and a hyperimmune rabbit serum on the hemopoieis, the white blood picture, and the morbidity rate in calves with bronchopneumonia. It was found that these preparations influence favourably the white blood picture, the clotting time of blood, and the resistance of calves. With the test calves the morbidity rate was lower than in the controls. Best prophylactic effect was produced with use of tissue preparations and lactoplasmin.

Medicina regenerativa II: aplicaciones, realidad y perspectivas de la terapia celular / Regenerative medicine II: applications, reality and perspectives of cell therapy

La medicina regenerativa ha surgido como una nueva disciplina médica impulsada fundamentalmente por los nuevos conocimientos sobre las células madre y en su capacidad de convertirse en células de diferentes tejidos. Un aspecto que se debe destacar y que conforma el elemento básico de este tipo de medicina, es que se apoya en los mismos factores intra e intercelulares que el organismo emplea para su autorreparación. Esta se sustenta en la terapia celular, en la administración de elementos subcelulares y en la ingeniería de tejidos, conductas usadas para remplazar por células sanas a las células dañadas por diversos procesos en determinados tejidos. En resumen, ella integra todos los procederes destinados a la promoción de la regeneración celular. En la actualidad, la terapéutica celular es uno de lo temas más excitantes de la medicina contemporánea. En los últimos tiempos, las investigaciones y aplicaciones preclínicas y clínicas de las células madre adultas, en particular las hematopoyéticas, han avanzado rápidamente, fundamentalmente teniendo en cuenta las dificultades surgidas con la obtención y empleo de las células madre embrionarias. Entre las enfermedades en las que se plantean posibles beneficios se encuentran las cardiovasculares, las arteriopatías periféricas, enfermedades neurológicas degenerativas, diabetes mellitus, enfermedades y lesiones óseas, y lesiones de la córnea, entre otras. El objetivo de este artículo es la actualización de los trabajos desarrollados con la aplicación de las células madre adultas en diferentes enfermedades

Células-tronco: um novo caminho para a humanidade e um novo cuidado para os enfermeiros-um estudo de caso / Stemcell therapy for cardiolgy: a new path to humanity and a new care for nurses-a case report

Durante o atendimento realizado a pacientes portadores de Infarto Agudo do Miocárdio (IAM), percebemos a complexidade de sua patologia. Atualmente com a utilização da terapia celular houve uma nova esperança para pacientes com este diagnóstico. Assim realizamos este estudo para aprimorarmos nossos conhecimentos, enquanto enfermeiros, à referida terapia e seus supostos benefícios.Trata-se do relato de caso de um paciente admitido em uma Unidade Coronariana(UCOR) com diagnóstico de Infarto Agudo do Miocárdio (IAM) anterior extenso e sua inclusão no protocolo para manipulação de Células Tronco (CT) de medula óssea (MO). O enfermeiro tem papel fundamental no atendimento deste paciente, esclarecendo suas dúvidas, atendendo expectativas, além de manter participação ativa nos procedimentos intra-hospitalares. A coleta de dados traduziu-se em pesquisa em prontuário e observação da evolução clinica. Durante todo este processo priorizamos as ações do nosso cuidado tentando avaliar as necessidades apresentadas pelo paciente.

During the process of care of patients who suffered acute myocardial infarction we observed how complex is this disease. Nowadays, stemcell therapy opens a new horizon and hope for these patients. We conducted this study to improve our knowledge as nurses in face of this new therapeutic model. This study is a case report of a patient who suffered an anterior acute myocardial infarction and was admitted in a coronary care unit and enrolled in stemcell transplantation study protocol. The nurse has a fundamental role in the process of care during the hospitalization period clarifying the patient's doubts and perspectives. The data was collected in patient's record.During the patient length of stay we emphasized our nursing care based on his individual value.

Uso de terapia regenerativa com células-tronco da medula óssea em doencas cardiovasculares: perspectiva do hematologista / Regenerative therapy using bone marrow stem cells in cardiovascular diseases: the perspective of the hematologist

O desenvolvimento de técnicas de transplante de órgãos sólidos e de medula óssea foi um dos mais fascinantes avancos da medicina no século XX. A virada do século XXI testemunha um desdobramento também fascinante e promissor desta modalidade terapêutica: o uso de células-tronco para regenerar tecidos lesados outrora considerados irreparáveis. Resultados encorajadores de inúmeros estudos com animais de experimentacão impulsionaram grupos de diversos centros no mundo a iniciar estudos clínicos com transplante de células-tronco em várias doencas, particularmente as doencas cardiovasculares e neurológicas. Embora ainda estejamos algo distante de entender o mecanismo preciso pelo qual as células-tronco regeneram órgãos lesados, os estudos publicados até o presente momento, incluindo vários estudos envolvendo seres humanos, sugerem haver um benefício real com esta terapia. O presente artigo pretende abordar os aspectos relevantes da terapia celular em doencas cardiovasculares, incluindo conceitos básicos sobre células-tronco, e os principais estudos de animais de experimentacão e clínicos publicados até o presente.