RESUMEN
Measles is one of the most infectious diseases of humans. It is caused by the measles virus (MeV) and can lead to serious illness, lifelong complications, and even death. Whole-genome sequencing (WGS) is now available to study molecular epidemiology and identify MeV transmission pathways. In the present study, WGS of 23 MeV strains of genotype H1, collected in Mainland China between 2006 and 2018, were generated and compared to 31 WGSs from the public domain to analyze genomic characteristics, evolutionary rates and date of emergence of H1 genotype. The noncoding region between M and F protein genes (M/F NCR) was the most variable region throughout the genome. Although the nucleotide substitution rate of H1 WGS was around 0.75 × 10-3 substitution per site per year, the M/F NCR had an evolutionary rate three times higher, with 2.44 × 10-3 substitution per site per year. Phylogenetic analysis identified three distinct genetic groups. The Time of the Most Recent Common Ancestor (TMRCA) of H1 genotype was estimated at approximately 1988, while the first genetic group appeared around 1995 followed by two other genetic groups in 1999-2002. Bayesian skyline plot showed that the genetic diversity of the H1 genotype remained stable even though the number of MeV cases decreased 50 times between 2014 (52 628) and 2020 (993). The current coronavirus disease 2019 (COVID-19) pandemic might have some effect on the measles epidemic and further studies will be necessary to assess the genetic diversity of the H1 genotype in a post-COVID area.
Asunto(s)
Evolución Molecular , Genoma Viral/genética , Virus del Sarampión/genética , China/epidemiología , Genes Virales/genética , Variación Genética , Genómica , Genotipo , Humanos , Sarampión/epidemiología , Sarampión/virología , Virus del Sarampión/clasificación , Filogenia , ARN Viral/genéticaRESUMEN
The increase of the evolutionary pressure will cause phylodynamics changes of viruses. In post-vaccine coverage era, measles viruses face more immune pressure than ever before. Vice versa, the phylodynamic changes may reflect herd immunity level provided by vaccination. In this study, we analyzed phylodynamic characteristics of measles viruses isolated from 2005 to 2017 in Jiangsu province of China using nucleoprotein gene sequences of measles viruses. The phylogenetic tree was constructed with Markov chain Monte Carlo algorithm. The mean gene distance within each group was computed with MEGA7.0 software. Our results showed that a decline trend is observed in the gene distance of nucleoprotein gene with time as well as incidence of measles from epidemic surveillance system. Two clusters of H1a genotype show cocirculation of multiple variants in early years and the disappearance of most variants with time. We explore the phylodynamic of measles virus under high immune pressure. Our findings highlight that phylodynamic of measles viruses is a helpful tool to assess the effectiveness of epidemic control.
Asunto(s)
Vacuna Antisarampión/inmunología , Virus del Sarampión/genética , Sarampión/epidemiología , Sarampión/virología , China/epidemiología , Genotipo , Humanos , Incidencia , Sarampión/prevención & control , Virus del Sarampión/clasificación , Virus del Sarampión/inmunología , Epidemiología Molecular , Proteínas de la Nucleocápside/genética , FilogeniaRESUMEN
Of the 24 known measles genotypes, only D8 and B3 are responsible for outbreaks in the last years in Europe, Asia, and America. In this study the H gene of 92 strains circulating between 2015 and 2019 in Lombardy, Northern Italy, and 1273 H sequences available in GenBank were analyzed in order to evaluate the genetic variability and to assess the conservation of the immunodominant sites. Overall, in Lombardy we observed the presence of four different B3 and three different D8 clusters, each one of them including sequences derived from viruses found in both vaccinated and unvaccinated subjects. Worldwide, the residue 400 within the H protein, a position located within the main immune epitope, is mutated in all circulating strains that belong to the two globally endemic genotypes, B3 and D8. Our data demonstrate the usefulness of measles virus (MV) H gene sequencing. Indeed, the monitoring the H protein epitopes of circulating strains could be included in the measles laboratory surveillance activities in order to improve and optimize strategies for measles control, as countries go towards elimination phase.
Asunto(s)
Variación Genética , Hemaglutininas/genética , Virus del Sarampión/genética , Sarampión/epidemiología , Epidemiología Molecular , Filogenia , Proteínas Virales/genética , Secuencia de Aminoácidos , Genotipo , Hemaglutininas/inmunología , Humanos , Italia/epidemiología , Sarampión/prevención & control , Sarampión/virología , Virus del Sarampión/clasificación , Virus del Sarampión/inmunología , Análisis de Secuencia de ADN , Homología de Secuencia , Vacunas Virales/administración & dosificaciónRESUMEN
In 2017, Italy experienced a large measles epidemic with 5408 cases and four deaths. As Subnational Reference Laboratory of the Measles and Rubella surveillance NETwork (MoRoNET), the EpiSoMI (Epidemiology and Molecular Surveillance of Infections) Laboratory (University of Milan) set up rapid and active surveillance for the complete characterisation of the Measles virus (Mv) responsible for the large measles outbreak in Milan and surrounding areas (Lombardy, Northern Italy). The aims of this study were to describe the genetic profile of circulating viruses and to track the pathway of measles transmission. Molecular analysis was performed by sequencing the highly variable 450 nucleotides region of the N gene (N-450) of Mv genome. Two-hundred and ninety-nine strains of Mv were analysed. The phylogenetic analysis showed five different variants, two not previously described in the studied area, belonging to D8 and B3 genotypes. Three events of continuous transmission of autochthonous variants (D8-Osaka, D8-London and B3-Milan variants) and two events of continuous transmission of imported variants (B3-Dublin and D8-Hulu Langat) tracked five different transmission pathways. These pathways outlined two epidemic peaks: the first in April and the second in July 2017. The correlation between Mv variant and the epidemiological data may enable us to identify the sources of virus importation and recognise long-lasting virus transmission pathways.
Asunto(s)
Epidemias , Genotipo , Virus del Sarampión/genética , Sarampión/epidemiología , Humanos , Italia/epidemiología , Sarampión/virología , Virus del Sarampión/clasificación , FilogeniaRESUMEN
Rapid differentiation of wild-type measles virus from measles vaccine strains is crucial during a measles outbreak and in a measles elimination setting. A real-time reverse transcription-PCR (rRT-PCR) for the rapid detection of measles vaccine strains was developed with high specificity and sensitivity equivalent to that of traditional measles genotyping methods. The "stressed" minor groove binder-TaqMan probe design approach achieves specificity to vaccine strains only, without compromising sensitivity. This assay, without requiring sequence genotyping, has proved to be extremely useful in outbreak settings for over 4 years at the Regional Measles Reference Laboratory for the Western Pacific Region.
Asunto(s)
Técnicas de Genotipaje/métodos , Vacuna Antisarampión/genética , Virus del Sarampión/genética , Sarampión/diagnóstico , Polimorfismo de Nucleótido Simple/genética , Reacción en Cadena en Tiempo Real de la Polimerasa , Brotes de Enfermedades , Genotipo , Técnicas de Genotipaje/normas , Humanos , Sarampión/epidemiología , Virus del Sarampión/clasificación , Proteínas de la Nucleocápside/genética , Estados del Pacífico/epidemiología , Reproducibilidad de los Resultados , Sensibilidad y EspecificidadRESUMEN
The measles virus hemagglutinin (MeV-H) protein is the main target of protective neutralizing antibodies. Using a panel of monoclonal antibodies (MAbs) that recognize known major antigenic sites in MeV-H, we identified a D4 genotype variant that escapes neutralization by MAbs targeting the neutralizing epitope (NE) antigenic site. By site-directed mutagenesis, L249P was identified as the critical mutation disrupting the NE in this genotype D4 variant. Forty-two available D4 genotype gene sequences were subsequently analyzed and divided into 2 groups according to the presence or absence of the L249P MeV-H mutation. Further analysis of the MeV-N gene sequences of these 2 groups confirmed that they represent clearly definable, sequence-divergent D4 subgenotypes, which we named subgenotypes D4.1 and D4.2. The subgenotype D4.1 MeVs were isolated predominantly in Kenya and Ethiopia, whereas the MAb-resistant subgenotype D4.2 MeVs were isolated predominantly in France and Great Britain, countries with higher vaccine coverage rates. Interestingly, D4.2 subgenotype viruses showed a trend toward diminished susceptibility to neutralization by human sera pooled from approximately 60 to 80 North American donors. Escape from MAb neutralization may be a powerful epidemiological surveillance tool to monitor the evolution of new MeV subgenotypes.IMPORTANCE Measles virus is a paradigmatic RNA virus, as the antigenic composition of the vaccination has not needed to be updated since its discovery. The vaccine confers protection by inducing neutralizing antibodies that interfere with the function of the hemagglutinin protein. Viral strains are indistinguishable serologically, although characteristic nucleotide sequences differentiate 24 genotypes. In this work, we describe a distant evolutionary branch within genotype D4. Designated subgenotype D4.2, this virus is distinguishable by neutralization with vaccine-induced monoclonal antibodies that target the neutralizing epitope (NE). The subgenotype D4.2 viruses have a higher predominance in countries with intermediary levels of vaccine coverage. Our studies demonstrate that subgenotype D4.2 lacks epitopes associated with half of the known antigenic sites, which significantly impacts our understanding of measles virus evolution.
Asunto(s)
Variación Antigénica , Epítopos/inmunología , Hemaglutininas Virales/inmunología , Virus del Sarampión/clasificación , Virus del Sarampión/genética , Anticuerpos Monoclonales/inmunología , Anticuerpos Neutralizantes/inmunología , Anticuerpos Antivirales/inmunología , Etiopía , Genotipo , Hemaglutininas Virales/genética , Humanos , Evasión Inmune , Kenia , Sarampión/virología , Vacuna Antisarampión/inmunología , Virus del Sarampión/inmunología , Mutagénesis Sitio-Dirigida , Pruebas de Neutralización , Reino Unido , Vacunación , Proteínas Virales/genética , Proteínas Virales/inmunologíaRESUMEN
Despite the availability of an effective vaccine, the measles virus continues to cause significant morbidity and mortality in children worldwide. Molecular characterization of wild-type measles strains is an invaluable component of epidemiological studies or surveillance systems that provides important information pertinent to outbreak linkages and transmission pathways. Serum samples and throat swabs were collected from suspected measles cases from the Punjab province of Pakistan (2013-2015) and further tested for measles immunoglobulin M (IgM) through enzyme-linked immunosorbent assay and reverse-transcriptase polymerase chain reaction for molecular characterization. Among the total of 5415 blood samples, 59% tested positive for measles IgM. Males had a higher infection rate (55%) than females (45%), and the highest frequency of positive cases (63%) was found in the age group of 0 to 5 years. Partial sequencing of the nucleoprotein gene showed that 27 strains belonged to the B3 genotype, whereas 2 viruses were identified as D4. On phylogenetic analysis, Pakistani B3 strains were found to be closely related to previously reported indigenous strains and those from neighboring countries of Iran and Qatar. This is the first report on the detection of the measles B3 genotype from Punjab, Pakistan. The current study shows a high burden of measles infections in Punjab province owing to poor routine immunization coverage in major cities. It is imperative that national health authorities adopt strategic steps on an urgent basis for improvement of routine immunization coverage. Molecular epidemiology of the measles viruses circulating in different parts of the country can provide useful data to manage future outbreaks.
Asunto(s)
Brotes de Enfermedades , Genotipo , Virus del Sarampión/clasificación , Virus del Sarampión/genética , Sarampión/epidemiología , Adolescente , Factores de Edad , Anticuerpos Antivirales/sangre , Niño , Preescolar , Transmisión de Enfermedad Infecciosa , Ensayo de Inmunoadsorción Enzimática , Femenino , Humanos , Inmunoglobulina M/sangre , Lactante , Recién Nacido , Masculino , Virus del Sarampión/aislamiento & purificación , Epidemiología Molecular , Proteínas de la Nucleocápside , Nucleoproteínas/genética , Pakistán/epidemiología , Faringe/virología , Filogenia , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Análisis de Secuencia de ADN , Suero/virología , Factores Sexuales , Proteínas Virales/genética , Adulto JovenRESUMEN
Strains of measles virus of genotypes D5, H1, D4, D8, and B3 were detected among epidemic, endemic, imported and import-associated cases in Hokkaido district, Japan, during 2006-2015. In the present study, their antigenic features were evaluated by determining the complete nucleotide sequences of their hemagglutinin proteins, which are a major target for neutralizing antibodies, and their amino acid sequences deduced. It was found that the hemagglutinin proteins of these strains had several novel amino acid changes in some functional regions. Although these strains have not caused further infections thus far, these antigenic changes should continue to be monitored to maintain their elimination status.
Asunto(s)
Variación Genética/genética , Hemaglutininas Virales/genética , Hemaglutininas Virales/inmunología , Virus del Sarampión/genética , Sarampión/inmunología , Sarampión/virología , Secuencia de Aminoácidos , Anticuerpos Neutralizantes , Anticuerpos Antivirales/sangre , Anticuerpos Antivirales/inmunología , Antígenos Virales/genética , Antígenos Virales/inmunología , Evolución Molecular , Genotipo , Humanos , Japón/epidemiología , Sarampión/epidemiología , Virus del Sarampión/clasificación , Epidemiología Molecular , Pruebas de Neutralización , Proteínas de la Nucleocápside/genética , Proteínas de la Nucleocápside/inmunología , Filogenia , Análisis de Secuencia de ADN , Proteínas Virales/genéticaRESUMEN
In September 2016, an imported case of measles in Edinburgh in a university student resulted in a further 17 confirmed cases during October and November 2016. All cases were genotype D8 and were associated with a virus strain most commonly seen in South East Asia. Twelve of the 18 cases were staff or students at a university in Edinburgh and 17 cases had incomplete or unknown measles mumps rubella (MMR) vaccination status. The public health response included mass follow-up of all identified contacts, widespread communications throughout universities in Edinburgh and prompt vaccination clinics at affected campuses. Imported cases of measles pose a significant risk to university student cohorts who may be undervaccinated, include a large number of international students and have a highly mobile population. Public health departments should work closely with universities to promote MMR uptake and put in place mass vaccination plans to prevent rapidly spreading measles outbreaks in higher educational settings in future.
Asunto(s)
Enfermedades Transmisibles Importadas/diagnóstico , Enfermedades Transmisibles Importadas/transmisión , Brotes de Enfermedades , Sarampión/epidemiología , Sarampión/transmisión , Universidades , Adolescente , Adulto , Niño , Preescolar , Personal Docente , Asia Oriental , Femenino , Genotipo , Técnicas de Genotipaje , Humanos , Lactante , Masculino , Virus del Sarampión/clasificación , Virus del Sarampión/genética , Virus del Sarampión/aislamiento & purificación , Escocia/epidemiología , Estudiantes , Adulto JovenRESUMEN
Measles is an important childhood infection targeted to be eliminated by the World Health Organization (WHO). Virus circulation has not been interrupted in the European Region because high vaccination rates could not be achieved among some countries of the WHO European Region including Turkey. The purpose of this study was to evaluate the laboratory findings of measles cases confirmed in the last nine years, to assess the epidemiological data of the cases, to determine the molecular genotyping studies and to emphasise the importance of laboratory-based surveillance in measles. From 2007 to 2010, only 18 imported cases were detected in Turkey. However, this number increased with a local outbreak of 111 cases in 2011, followed by another outbreak in 2012 in Istanbul that spread countrywide in the following two years; a total of 8661 laboratory-confirmed measles cases were reported from 2012 to 2015. After ELISA detection of a measles IgM-positive result in serum samples of potential measles cases, RT-PCR was performed with urine or nasopharyngeal swab samples of patients, and amplicons were subjected to sequencing. In the samples of 2010 and 2011, D4 and D9 genotypes were mainly detected; as of 2012, the D8 genotype has gained importance. Although D8 was also identified in 2014, in the same year genotype H1 viruses were detected in Turkey for the first time. Therefore, it is important to perform a genotypic analysis of the virus causing the outbreak, analyse epidemiological connections of the contact, determine the source of the outbreak and plan measures based on this information.
Asunto(s)
Erradicación de la Enfermedad/métodos , Sarampión/diagnóstico , Sarampión/epidemiología , Técnicas de Diagnóstico Molecular/métodos , Pruebas Serológicas/métodos , Anticuerpos Antivirales/sangre , Niño , Preescolar , Enfermedades Transmisibles Importadas/epidemiología , Brotes de Enfermedades , Ensayo de Inmunoadsorción Enzimática/métodos , Genotipo , Técnicas de Genotipaje/métodos , Humanos , Inmunoglobulina M/sangre , Lactante , Virus del Sarampión/clasificación , Virus del Sarampión/genética , Virus del Sarampión/inmunología , Virus del Sarampión/aislamiento & purificación , Epidemiología Molecular/métodos , Nasofaringe/virología , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Análisis de Secuencia de ADN , Turquía/epidemiología , Orina/virologíaRESUMEN
Endemic measles transmission was interrupted for the first time in Ireland in 2015. In May 2016, a case of measles was confirmed in an adult who had travelled from Hungary to Ireland (index case). Cases subsequently arose in five of the eight public health regions around the country. There were 40 confirmed cases in Ireland between April and September 2016. All sequenced cases were genotype B3. Vaccination status was known for 34 cases, of whom 31 were unvaccinated. Median age was 8 years (range: 3 months to 40 years). Ten cases were nosocomial, and three cases were infected on separate international flights. One linked case occurred in a resident of Slovenia. Nineteen cases were hospitalised; median duration of hospitalisation was 5 days (range: 2-8 days). The primary case was a child who travelled from Romania to Ireland via Budapest, and infected the index adult case on the same flight. This was the first reported outbreak of measles genotype B3 in Ireland. This outbreak demonstrated that Ireland remains at risk of measles outbreaks due to persistent suboptimal vaccination rates.
Asunto(s)
Brotes de Enfermedades/estadística & datos numéricos , Enfermedades Endémicas/estadística & datos numéricos , Vacunación Masiva/estadística & datos numéricos , Virus del Sarampión/genética , Virus del Sarampión/aislamiento & purificación , Vacuna contra el Sarampión-Parotiditis-Rubéola/administración & dosificación , Sarampión/epidemiología , Sarampión/transmisión , ARN Viral/genética , Adolescente , Adulto , Niño , Preescolar , Trazado de Contacto , Brotes de Enfermedades/prevención & control , Enfermedades Endémicas/prevención & control , Femenino , Humanos , Lactante , Recién Nacido , Irlanda/epidemiología , Masculino , Sarampión/diagnóstico , Virus del Sarampión/clasificación , Vacuna contra el Sarampión-Parotiditis-Rubéola/uso terapéutico , Vigilancia de la Población , Reacción en Cadena en Tiempo Real de la Polimerasa , Viaje , Adulto JovenRESUMEN
The province of Ontario continues to experience measles virus transmissions despite the elimination of measles in Canada. We describe an unusual outbreak of measles in Ontario, Canada, in early 2015 that involved cases with a unique strain of virus and no known association among primary case-patients. A total of 18 cases of measles were reported from 4 public health units during the outbreak period (January 25-March 23, 2015); none of these cases occurred in persons who had recently traveled. Despite enhancements to case-patient interview methods and epidemiologic analyses, a source patient was not identified. However, the molecular epidemiologic analysis, which included extended sequencing, strongly suggested that all cases derived from a single importation of measles virus genotype D4. The use of timely genotype sequencing, rigorous epidemiologic investigation, and a better understanding of the gaps in surveillance are needed to maintain Ontario's measles elimination status.
Asunto(s)
Brotes de Enfermedades , Genotipo , Virus del Sarampión/genética , Sarampión/epidemiología , Sarampión/virología , Adolescente , Adulto , Niño , Femenino , Historia del Siglo XXI , Humanos , Masculino , Sarampión/diagnóstico , Sarampión/historia , Virus del Sarampión/clasificación , Ontario/epidemiología , Vigilancia en Salud Pública , ARN Viral/genética , Análisis de Secuencia de ADN , Serogrupo , Vacunación , Adulto JovenRESUMEN
During measles outbreaks, it is important to be able to rapidly distinguish between measles cases and vaccine reactions to avoid unnecessary outbreak response measures such as case isolation and contact investigations. We have developed a real-time reverse transcription-PCR (RT-PCR) method specific for genotype A measles virus (MeV) (MeVA RT-quantitative PCR [RT-qPCR]) that can identify measles vaccine strains rapidly, with high throughput, and without the need for sequencing to determine the genotype. We have evaluated the method independently in three measles reference laboratories using two platforms, the Roche LightCycler 480 system and the Applied Biosystems (ABI) 7500 real-time PCR system. In comparison to the standard real-time RT-PCR method, the MeVA RT-qPCR showed 99.5% specificity for genotype A and 94% sensitivity for both platforms. The new assay was able to detect RNA from five currently used vaccine strains, AIK-C, CAM-70, Edmonston-Zagreb, Moraten, and Shanghai-191. The MeVA RT-qPCR assay has been used successfully for measles surveillance in reference laboratories, and it could be readily deployed to national and subnational laboratories on a wide scale.
Asunto(s)
Genotipo , Vacuna Antisarampión/genética , Virus del Sarampión/clasificación , Virus del Sarampión/genética , Reacción en Cadena en Tiempo Real de la Polimerasa/métodos , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Humanos , Virus del Sarampión/aislamiento & purificación , Sensibilidad y EspecificidadRESUMEN
The Government of India is accepted to participate in the measles elimination and rubella control goal 2020, hence genetic characterization of measles viruses (MeV) becomes essential. At National Reference Laboratory (National Institute of Virology, Pune), the throat swabs/urine specimens (n = 380) or PCR products (n = 219) obtained from the suspected measles cases were referred for the molecular testing and subsequently, MeV nucleoprotein (N) gene sequencing/genotyping. In addition, 2,449 suspected measles cases, mainly from the Maharashtra state were referred for the laboratory diagnosis. A detailed study was performed on N gene sequences obtained during last two decades. Indian MeV sequences obtained during 2011-2015 were compared with 1996-2010 sequences and genetic divergence was studied. Circulation of measles genotypes B3 (n = 3), D4 (n = 49), and D8 (n = 351) strains were observed in 19 States and three Union Territories of India. In addition, 64 measles viruses were isolated from 253 throat swab or urine specimens obtained from the suspected measles cases. During 2011-2015, 67.9% (1,663/2,449) suspected measles cases were laboratory confirmed. Molecular studies showed circulation of measles genotype B3 in India along with prominently circulating genotypes D4 and D8 except D7 strains. The genetic diversion within Indian B3, D4, and D8 genotypes was 0.3%, 1.1%, and 2.1%, respectively. The genetic divergence of Indian B3, D4, and D8 measles strains with the WHO reference sequences was 2.5%, 2.6%, and 1.8%, respectively. It is crucial data for national immunization program. More measles/rubella genotyping studies are necessary to track transmission and to support measles elimination and rubella control. J. Med. Virol. 89:753-758, 2017. © 2016 Wiley Periodicals, Inc.
Asunto(s)
Genotipo , Virus del Sarampión/clasificación , Virus del Sarampión/genética , Sarampión/epidemiología , Sarampión/virología , Adolescente , Adulto , Niño , Preescolar , Femenino , Humanos , India/epidemiología , Lactante , Masculino , Virus del Sarampión/aislamiento & purificación , Epidemiología Molecular , Faringe/virología , Reacción en Cadena de la Polimerasa , ARN Viral/genética , Análisis de Secuencia de ADN , Orina/virología , Adulto JovenRESUMEN
RNA viruses usually consist of mutant spectra because of high error rates of viral RNA polymerases. Growth competition occurs among different viral variants, and the fittest clones predominate under given conditions. Individual variants, however, may not be entirely independent of each other, and internal interactions within mutant spectra can occur. Examples of cooperative and interfering interactions that exert enhancing and suppressing effects on replication of the wild-type virus, respectively, have been described, but their underlying mechanisms have not been well defined. It was recently found that the cooperation between wild-type and variant measles virus genomes produces a new phenotype through the heterooligomer formation of a viral protein. This observation provides a molecular mechanism underlying cooperative interactions within mutant spectra. Careful attention to individual sequences, in addition to consensus sequences, may disclose further examples of internal interactions within mutant spectra.
Asunto(s)
Virus del Sarampión/fisiología , Mutación , Animales , Evolución Molecular , Humanos , Sarampión/virología , Virus del Sarampión/clasificación , Virus del Sarampión/genética , Modelos Genéticos , Proteínas Virales/genética , Proteínas Virales/metabolismo , Replicación ViralRESUMEN
Measles continues to be a major public health issue causing substantial outbreaks worldwide, mostly affecting young children. Molecular analysis of measles viruses provides important information on outbreak linkages and transmission pathways that can be helpful towards implementation of appropriate control programs. In Pakistan, the control of measles is still tenuous, and progress towards elimination has been irregular and challenging. In the 2013 measles outbreak we received 4,682 sera collected from suspected patients in 23 districts across Sindh. A total of 3,283 samples were confirmed measles positive using IgM ELISA with the highest infection rate in children aged 1-12 months. Males were more affected than females and a visible peak was observed from January to April. Among the 3,283 cases, 59.1% were unvaccinated, 29.6% had received 1 dose and 10.3% had received 2 doses of measles vaccine while 0.85% had an unknown vaccination status. For genotype detection and phylogenetic analysis, 60 throat swab samples were collected from suspected patients below 15 years of age in eight districts of Sindh province. Forty four (73%; 44/60) throat swab samples were successfully genotyped using RT-PCR. Phylogenetic analyses based on partial sequences of the nucleocapsid protein gene revealed that all Pakistani measles virus strains belonged to genotype B3 and were closely related to those isolated from neighboring countries such as Iran, Afghanistan (99.1-100%) and India with 98.6 - 99.6% nucleotide homology. This is the first report on the phylogenetic analysis of measles B3 genotype strains from Pakistan and highlights the need for strengthening the surveillance systems and improving immunization coverage across the country.
Asunto(s)
Brotes de Enfermedades , Genotipo , Virus del Sarampión/clasificación , Virus del Sarampión/aislamiento & purificación , Sarampión/epidemiología , Sarampión/virología , Adolescente , Adulto , Distribución por Edad , Anticuerpos Antivirales/sangre , Niño , Preescolar , Ensayo de Inmunoadsorción Enzimática , Femenino , Técnicas de Genotipaje , Humanos , Inmunoglobulina M/sangre , Lactante , Masculino , Vacuna Antisarampión/administración & dosificación , Virus del Sarampión/genética , Persona de Mediana Edad , Pakistán/epidemiología , Filogenia , ARN Viral/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Análisis de Secuencia de ADN , Factores Sexuales , Vacunación/estadística & datos numéricos , Adulto JovenRESUMEN
One imported and five secondary cases of measles were detected in Finland between June and August 2017. The measles sequences available for five laboratory-confirmed cases were identical and belonged to serotype D8. The large number of potentially exposed Finnish and foreign individuals called for close cooperation of national and international public health authorities and other stakeholders. Raising awareness among healthcare providers and ensuring universally high vaccination coverage is crucial to prevent future clusters and outbreaks.
Asunto(s)
Trazado de Contacto , Brotes de Enfermedades , Virus del Sarampión/aislamiento & purificación , Sarampión/epidemiología , Viaje , Adolescente , Adulto , Brotes de Enfermedades/prevención & control , Finlandia/epidemiología , Humanos , Sarampión/diagnóstico , Sarampión/transmisión , Virus del Sarampión/clasificación , Salud Pública , Serogrupo , Serotipificación , VacunaciónRESUMEN
The largest measles outbreak in Berlin since 2001 occurred from October 2014 to August 2015. Overall, 1,344 cases were ascertained, 86% (with available information) unvaccinated, including 146 (12%) asylum seekers. Median age was 17 years (interquartile range: 4-29 years), 26% were hospitalised and a 1-year-old child died. Measles virus genotyping uniformly revealed the variant 'D8-Rostov-Don' and descendants. The virus was likely introduced by and initially spread among asylum seekers before affecting Berlin's resident population. Among Berlin residents, the highest incidence was in children aged < 2 years, yet most cases (52%) were adults. Post-exposure vaccinations in homes for asylum seekers, not always conducted, occurred later (median: 7.5 days) than the recommended 72 hours after onset of the first case and reached only half of potential contacts. Asylum seekers should not only have non-discriminatory, equitable access to vaccination, they also need to be offered measles vaccination in a timely fashion, i.e. immediately upon arrival in the receiving country. Supplementary immunisation activities targeting the resident population, particularly adults, are urgently needed in Berlin.
Asunto(s)
Control de Enfermedades Transmisibles , Brotes de Enfermedades/prevención & control , Virus del Sarampión/genética , Refugiados/estadística & datos numéricos , Vacunación/estadística & datos numéricos , Adolescente , Adulto , Berlin/epidemiología , Niño , Preescolar , Brotes de Enfermedades/estadística & datos numéricos , Emigración e Inmigración/estadística & datos numéricos , Femenino , Genotipo , Humanos , Incidencia , Masculino , Exámenes Obligatorios/estadística & datos numéricos , Sarampión/epidemiología , Virus del Sarampión/clasificación , Virus del Sarampión/aislamiento & purificación , Adulto JovenRESUMEN
From March to August 2017, 165 measles cases were reported from three regions in Bulgaria. The age range was 0-55 years and 66% of the cases were under 9 years. The Roma population was disproportionally affected (89% of cases), 41% cases were unvaccinated and in 24 cases there was nosocomial transmission mostly in paediatric departments. A child under 12 months of age died. Control measures have been taken and the investigation is still ongoing.
Asunto(s)
Infección Hospitalaria/epidemiología , Brotes de Enfermedades , Transmisión de Enfermedad Infecciosa de Paciente a Profesional/estadística & datos numéricos , Virus del Sarampión/aislamiento & purificación , Sarampión/etnología , Sarampión/transmisión , Romaní , Vacunación/estadística & datos numéricos , Adolescente , Adulto , Distribución por Edad , Bulgaria/epidemiología , Niño , Preescolar , Brotes de Enfermedades/prevención & control , Estudios Epidemiológicos , Femenino , Genotipo , Humanos , Lactante , Masculino , Sarampión/virología , Vacuna Antisarampión/administración & dosificación , Virus del Sarampión/clasificación , Virus del Sarampión/genética , Persona de Mediana Edad , Filogenia , Vigilancia de la Población , Factores de Riesgo , Adulto JovenRESUMEN
The United Kingdom achieved interrupted endemic measles transmission for 36 months in 2016. Despite this, ongoing challenges from sporadic measles cases typically imported from abroad remain. We summarise a B3 measles genotype outbreak in south-east Wales occurring between May and September 2017, linked with other European outbreaks, and lessons learnt. Seventeen confirmed cases and one probable case occurred principally in education and healthcare-settings. Six confirmed cases attended healthcare settings when infectious, without being isolated.