QseC signaling in the outbreak O104:H4 escherichia coli strain combines multiple factors during infection
Journal of Bacteriology
; 201(17): e00203-19, 2019.
Article
en En
| SES-SP, SESSP-IBPROD, SES-SP
| ID: but-ib17170
Biblioteca responsable:
BR78.1
Ubicación: BR78.1
ABSTRACT
Enteroaggregative Escherichia coli (EAEC) from the O104H4 specific serotype caused a large outbreak of bloody diarrhea with some complicated cases of hemolytic-uremic syndrome (HUS) in Europe in 2011. The outbreak strain consisted in an EAEC capable to produce the Shiga toxin (Stx) subtype 2a, a characteristic from enterohemorrhagic E. coli. QseBC two-component system detects AI-3/Epi/NE and mediates the chemical signaling between pathogen and mammalian host. This system coordinates a cascade of virulence genes expression in important human enteropathogens. The blocking of QseC of EAEC C227-11 (Stx) strain by N-phenyl-4- {[(phenylamino) thioxomethyl]amino}-benzenesulfonamide (also known as LED209) in vivo demonstrated a lower efficiency of colonization. The periplasmic protein VisP, which is related to survival mechanisms in a colitis model of infection, bacterial membrane maintenance, and stress resistance, here presented high levels of expression during the initial infection within the host. Under acid stress conditions, visP expression levels were differentiated in an Stx-dependent way. Together, these results emphasize the important role of VisP and the histidine kinase sensor QseC in the C227-11 (Stx) outbreak strain for the establishment of the infectious niche process in the C57BL/6 mouse model and of LED209 as a promising antivirulence drug strategy against these enteric pathogens.
Texto completo:
1
Colección:
06-national
/
BR
Banco de datos:
SES-SP
/
SESSP-IBPROD
Idioma:
En
Revista:
Journal of Bacteriology
Año:
2019
Tipo del documento:
Article