The effect of omeprazole dosing on the isolation of Helicobacter pylori from gastric aspirates.
Aliment Pharmacol Ther
; 13(9): 1161-9, 1999 Sep.
Article
en En
| MEDLINE
| ID: mdl-10468697
ABSTRACT
BACKGROUND:
Animal experiments suggest that omeprazole dosing increases shedding of Helicobacter into the gastric lumen, and hence into gastric juice.AIM:
To assess the effect of omeprazole dosing on the yield of H. pylori from gastric aspirates of infected volunteers.METHODS:
Six serial nasogastric aspirates, three before and three during dosing with omeprazole 40 mg b.d., were obtained for culture from 10 H. pylori infected volunteers and one uninfected volunteer. To reduce contamination, samples were diluted 110 with Maximum Recovery Diluent (MRD; pH 7.0) or HCl-KCl buffer (pH 2.2) prior to culture on Columbia and Dent's agar.RESULTS:
Undiluted gastric juice cultures were rapidly overgrown by upper respiratory tract flora. HCl-KCl dilution resulted in isolation of H. pylori from 77% of infected subject aspirates before, and 67% of aspirates during dosing with omeprazole. The yields were significantly lower with MRD dilution, 47% and 10%, respectively. Omeprazole dosing significantly decreased the yield after MRD dilution, but not after HCl-KCl dilution.CONCLUSIONS:
Decreasing intragastric acidity, by dosing with omeprazole, decreases the isolation of H. pylori from routinely processed gastric aspirates. In vitro acidification of gastric aspirates, by HCl-KCl dilution, increases the isolation of H. pylori both before and during omeprazole dosing.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Omeprazol
/
Helicobacter pylori
/
Jugo Gástrico
/
Antiulcerosos
Tipo de estudio:
Clinical_trials
/
Risk_factors_studies
Límite:
Adult
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Aliment Pharmacol Ther
Asunto de la revista:
FARMACOLOGIA
/
GASTROENTEROLOGIA
/
TERAPIA POR MEDICAMENTOS
Año:
1999
Tipo del documento:
Article
País de afiliación:
Reino Unido