Mechanical barrier as a protection against rejection of allogeneic cartilage formed in joint surface defects in rats.
Cell Transplant
; 9(3): 349-57, 2000.
Article
en En
| MEDLINE
| ID: mdl-10972334
ABSTRACT
Cartilage formed in transplants of allogeneic chondrocytes into joint cartilage defects in rats was infiltrated by immune cells migrating from the bone marrow while the surface on the side of the joint cavity remained free of infiltrations. This suggested that immunization occurred via bone marrow and not via joint cavity. Because articular cartilage is nourished exclusively by the synovial fluid, we have attempted to prevent cartilage rejection by protecting transplants from the contact with bone marrow. Defects in articular surface were filled with bone cement and chondrocytes were transplanted into a cavity prepared within the bone cement plug. Cartilage formed within the cement shell remained free of infiltrations and did not evoke systemic immunological response. However, distribution of glycosaminoglycans in the matrix of protected transplants was irregular. Cultures of chondrocytes growing in vitro on cement contained less glycosaminoglycans than the controls. This suggests that some factor(s) released from the cement unfavorably influenced chondrocytes and matrix production in protected transplants.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Cementos para Huesos
/
Cartílago Articular
/
Condrocitos
Límite:
Animals
Idioma:
En
Revista:
Cell Transplant
Asunto de la revista:
TRANSPLANTE
Año:
2000
Tipo del documento:
Article
País de afiliación:
Polonia