A new phospholipase-C-calcium signalling pathway mediated by cyclic AMP and a Rap GTPase.
Nat Cell Biol
; 3(11): 1020-4, 2001 Nov.
Article
en En
| MEDLINE
| ID: mdl-11715024
ABSTRACT
Stimulation of phosphoinositide-hydrolysing phospholipase C (PLC) generating inositol-1,4,5-trisphosphate is a major calcium signalling pathway used by a wide variety of membrane receptors, activating distinct PLC-beta or PLC-gamma isoforms. Here we report a new PLC and calcium signalling pathway that is triggered by cyclic AMP (cAMP) and mediated by a small GTPase of the Rap family. Activation of the adenylyl cyclase-coupled beta2-adrenoceptor expressed in HEK-293 cells or the endogenous receptor for prostaglandin E1 in N1E-115 neuroblastoma cells induced calcium mobilization and PLC stimulation, seemingly caused by cAMP formation, but was independent of protein kinase A (PKA). We provide evidence that these receptor responses are mediated by a Rap GTPase, specifically Rap2B, activated by a guanine-nucleotide-exchange factor (Epac) regulated by cAMP, and involve the recently identified PLC-epsilon isoform.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fosfolipasas de Tipo C
/
AMP Cíclico
/
Señalización del Calcio
/
Proteínas de Unión al GTP rap
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Nat Cell Biol
Año:
2001
Tipo del documento:
Article
País de afiliación:
Alemania