Assessing allele frequencies of single nucleotide polymorphisms in DNA pools by pyrosequencing technology.
Biotechniques
; 32(5): 1144-6, 1148, 1150 passim, 2002 May.
Article
en En
| MEDLINE
| ID: mdl-12019788
ABSTRACT
Single nucleotide polymorphism (SNP) association studies searching for differences in allele frequencies between cases and controls have been widely used for genetic analysis. Individual genotyping is prohibitively expensive in large sample sizes. Pooling of samples provides the obvious advantage of higher throughput and lower cost. Here we report our results with the analysis of SNP allele frequencies in DNA pools using Pyrosequencing technology. For seven different SNPs, we observed a mean difference of 1.1 +/- 0.6% between allele frequencies determined in two different DNA pools (n = 150 cases and 150 controls) compared to individually genotyped samples.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Análisis de Secuencia de ADN
/
Polimorfismo de Nucleótido Simple
/
Frecuencia de los Genes
Límite:
Humans
Idioma:
En
Revista:
Biotechniques
Año:
2002
Tipo del documento:
Article
País de afiliación:
Estados Unidos