Cloning and Expression Analysis of a Laryngeal Carcinoma Related Gene, LCRG1.

By screening a human adult cDNA library using a cDNA fragment (AF10056) as a probe, which is significantly down-regulated in laryngeal carcinoma and represents a novel gene, a cDNA, LCRG1(laryngeal carcinoma related gene 1) was identified, which was significantly down-regulated in 12 of 30(40%) primary laryngeal carcinomas and in 6 of 11(54.5%) various cancer cell lines. This gene, localized on chromosome band 17q12--21.1 by alignment of it with STS markers, was composed of six exons and spaned about 60 kb of genomic DNA with a 3.4 kb mature transcript. The putative protein encoded by this gene was 288 amino acid with no significant homology with any known proteins in databases. LCRG1 was expressed in many tissues, as shown by MTN blot analysis. These data suggest that LCRG1 is related to the laryngeal carcinoma.