Novel polyamine coating providing non-covalent deactivation and reversed electroosmotic flow of fused-silica capillaries for capillary electrophoresis.
J Chromatogr A
; 1003(1-2): 217-21, 2003 Jun 27.
Article
en En
| MEDLINE
| ID: mdl-12899312
ABSTRACT
A new polycationic coating for use in capillary electrophoresis has been developed that enables chemical modification of fused-silica capillary surfaces for analysis of compounds like basic proteins. The cationic polyamine, containing short aliphatic blocks of combined 2 and 3-carbon length, was physically adsorbed onto the negatively charged fused-silica surface through ionic interaction by flushing the capillary with a polyamine solution, followed by a self-stabilization step. The polyamine coated capillaries generated an anodal electroosmotic flow that was independent of pH in the investigated range of pH 4-8. The capillary performance was demonstrated by fast separations of basic proteins with peak efficiencies in the range of 265,000-584,000 plates.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Poliaminas
/
Electroforesis Capilar
Idioma:
En
Revista:
J Chromatogr A
Año:
2003
Tipo del documento:
Article
País de afiliación:
Suecia