Usefulness of deoxyribonuclease I (DNase I) polymorphism for individualization from small aged urine stains.
Leg Med (Tokyo)
; 5(2): 105-7, 2003 Jun.
Article
en En
| MEDLINE
| ID: mdl-12935540
ABSTRACT
We devised a procedure that combines a simple extraction method, isoelectric focusing and activity staining using the dried agarose film overlay method, for deoxyribonuclease I (DNase I) typing from aged urine stains. DNase I types were determined without difficulty from urine stains kept at room temperature for 3 months or more in all of the samples tested. The amounts of urine stains required for typing after 3 months of storage were estimated to be equivalent to 60-120 microl of liquid urine. Therefore, considering that useful PCR-based DNA typing has not yet been developed for urine stains, DNase I polymorphism could be considered the first biochemical marker found to be well suited for individualization from small aged urine stains.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Desoxirribonucleasa I
Límite:
Humans
Idioma:
En
Revista:
Leg Med (Tokyo)
Asunto de la revista:
JURISPRUDENCIA
Año:
2003
Tipo del documento:
Article
País de afiliación:
Japón