Bovine papillomavirus replicative helicase E1 is a target of the ubiquitin ligase APC.
J Virol
; 78(5): 2615-9, 2004 Mar.
Article
en En
| MEDLINE
| ID: mdl-14963168
ABSTRACT
The papillomavirus E1 replicative helicase is essential for replication and maintenance of extrachromosomal viral genomes in infected cells. We previously found that the bovine papillomavirus E1 protein is a substrate of the ubiquitin-dependent proteolytic pathway. Here we show that E1 is targeted for degradation by the anaphase-promoting complex (APC). Inhibition of APC activity by the specific inhibitor Emi1 or point mutations in the D-box and KEN-box motifs of E1 stabilize the protein and increase viral DNA replication in both a cell-free system and in living cells. These findings involve APC as the ubiquitin ligase that controls E1 levels to maintain a constant low copy number of the viral genome during latent infection.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Virales
/
ADN Helicasas
/
Complejos de Ubiquitina-Proteína Ligasa
/
Ubiquitina-Proteína Ligasas
/
Proteínas de Unión al ADN
/
Papillomavirus Bovino 1
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Virol
Año:
2004
Tipo del documento:
Article
País de afiliación:
Francia