Survival of mouse blastocysts after low-temperature preservation under high pressure.
Acta Vet Hung
; 52(4): 479-87, 2004.
Article
en En
| MEDLINE
| ID: mdl-15595281
ABSTRACT
Cryoinjuries are almost inevitable during the freezing of embryos. The present study examines the possibility of using high hydrostatic pressure to reduce substantially the freezing point of the embryo-holding solution, in order to preserve embryos at subzero temperatures, thus avoiding all the disadvantages of freezing. The pressure of 210 MPa lowers the phase transition temperature of water to -21 degrees C. According to the results of this study, embryos can survive in high hydrostatic pressure environment at room temperature; the time embryos spend under pressure without significant loss in their survival could be lengthened by gradual decompression. Pressurisation at 0 degrees C significantly reduced the survival capacity of the embryos; gradual decompression had no beneficial effect on survival at that stage. Based on the findings, the use of the phenomena is not applicable in this form, since pressure and low temperature together proved to be lethal to the embryos in these experiments. The application of hydrostatic pressure in embryo cryopreservation requires more detailed research, although the experience gained in this study can be applied usefully in different circumstances.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Supervivencia Tisular
/
Blastocisto
/
Criopreservación
/
Presión Hidrostática
Límite:
Animals
Idioma:
En
Revista:
Acta Vet Hung
Año:
2004
Tipo del documento:
Article
País de afiliación:
Hungria