Verification of somatic CAG repeat expansion by pre-PCR fractionation.
J Neurosci Methods
; 144(1): 11-7, 2005 May 15.
Article
en En
| MEDLINE
| ID: mdl-15848234
ABSTRACT
The inheritance of a long CAG repeat causes several late onset neurological disorders including Huntington's disease (HD). Longer CAG repeats correlate with earlier onset of HD suggesting an increased toxicity for the products of long repeat alleles. PCR based data has been used to show that HD CAG repeat expansion beyond the inherited length occurs in affected tissues indicating a possible role for somatic instability in the disease process. PCR, however, is prone to artifacts resulting from expansion of repeat sequences during amplification. We describe a method to distinguish between CAG repeat expansions that exist in vivo and those that potentially occur during PCR. The method involves size fractionation of genomic restriction fragments containing the expanded repeats followed by PCR amplification. The application of this method confirms the presence of somatic expansions in the brains of a knock-in mouse model of HD.
Buscar en Google
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Reacción en Cadena de la Polimerasa
/
Enfermedad de Huntington
/
Expansión de Repetición de Trinucleótido
Límite:
Animals
Idioma:
En
Revista:
J Neurosci Methods
Año:
2005
Tipo del documento:
Article
País de afiliación:
Estados Unidos