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Bio-artificial periosteum for severe open fracture--an experimental study of osteogenic cell/collagen sponge composite as a bio-artificial periosteum.
Hattori, Koji; Yoshikawa, Takafumi; Takakura, Yoshinori; Aoki, Hideyuki; Sonobe, Masato; Tomita, Naohide.
Afiliación
  • Hattori K; Department of Orthopaedic Surgery, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8522, Japan. hattori@naramed-u.ac.jp
Biomed Mater Eng ; 15(3): 127-36, 2005.
Article en En | MEDLINE | ID: mdl-15911994
ABSTRACT
In an attempt to reduce complications in cases of severe open fracture, we developed a bio-artificial periosteum composed of osteogenic cells and collagen sponge. In the present study, we evaluated the osteogenic potential of the bio-artificial periosteum in vivo and in vitro. After 4-week incubation in vitro, the bio-artificial periosteum had high alkaline phosphatase activity and osteocalcin content. Moreover, energy dispersive X-ray analysis revealed numerous crystal structures consisting of P and Ca on the surface of the bio-artificial periosteum. Using a rat model for severe bone injury, we examined the bone formation process in defect sites covered with the bio-artificial periosteum. New bone formation occurred in the central part of the bone defect as well as at the bone edge. We conclude that by using the bio-artificial periosteum, the fracture site benefited from an improved osteogenic environment. These results indicate that a clinical trial to further evaluate this technique should be conducted.
Asunto(s)
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Periostio / Materiales Biocompatibles / Ingeniería Biomédica / Colágeno / Curación de Fractura / Sustitutos de Huesos / Fracturas Abiertas Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Biomed Mater Eng Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2005 Tipo del documento: Article País de afiliación: Japón
Buscar en Google
Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Periostio / Materiales Biocompatibles / Ingeniería Biomédica / Colágeno / Curación de Fractura / Sustitutos de Huesos / Fracturas Abiertas Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Biomed Mater Eng Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2005 Tipo del documento: Article País de afiliación: Japón