Er:YAG laser: antimicrobial effects in the root canals of dogs' teeth with pulp necrosis and chronic periapical lesions.

ABSTRACT

OBJECTIVE: Our goal in this study was to evaluate the antimicrobial effect of ErYAG laser applied after biomechanical preparation of the root canals of dog's teeth with apical periodontitis. BACKGROUND DATA Various in vitro studies have reported effective bacterial reduction in infected root canals using ErYAG laser. However, there is no in vivo research to support these results. METHODS: Forty root canals of dogs' premolar teeth with pulp necrosis and chronic periapical lesions were used. An initial microbiological sample was taken, and after biomechanical preparation was carried out, a second microbiological sample was taken. The teeth were divided into two groups Group I-biomechanical preparation was taken of root canals without ErYAG laser application; Group II-biomechanical preparation was taken of root canals with ErYAG laser application using 140-mJ input, 63-mJ output/15 Hz. After coronal sealing, the root canals were left empty for 7 days at which time a third microbiological sample was taken. The collected material was removed from the root canal with a #40 K file and placed in transport media. It was serially diluted and seeded on culture dishes selective for anaerobes, aerobes, and total streptococci. Colony-forming units per milliliter (CFU/mL) were counted. RESULTS: Groups I and II showed an increase of CFU/mL for all microorganisms 7 days after treatment, being statistically significant for anaerobes in Group I and for anaerobes and total streptococci in Group II. When comparing CFU/mL of Groups I and II, there was a statistically significant increase after 7 d for total streptococci in Group II. CONCLUSION: ErYAG laser applied after biomechanical preparation did not reduce microorganisms in the root canal system.