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Conjugated linoleic acid suppresses NF-kappa B activation and IL-12 production in dendritic cells through ERK-mediated IL-10 induction.
Loscher, Christine E; Draper, Eve; Leavy, Olive; Kelleher, Dermot; Mills, Kingston H G; Roche, Helen M.
Afiliación
  • Loscher CE; Department of Clinical Medicine, Institute of Molecular Medicine, Dublin, Ireland .
J Immunol ; 175(8): 4990-8, 2005 Oct 15.
Article en En | MEDLINE | ID: mdl-16210601
ABSTRACT
Polyunsaturated fatty acids (PUFA) have been shown to modulate immune responses and have therapeutic effects in inflammatory disorders. However, the influence of PUFA on dendritic cells (DC), key cells of the innate immune system in shaping adaptive immune responses, has not yet been defined. In this study, we examine the effects of the cis-9, trans-11 isomer of conjugated linoleic acid (c9, t11-CLA), a dietary PUFA found in meat and dairy products, on murine DC activation. Treatment of DC with c9, t11-CLA suppressed LPS-induced IL-12, enhanced IL-10R expression, and enhanced IL-10 production at the transcriptional and protein level. The suppression of IL-12 by c9, t11-CLA was found to be IL-10 dependent. We investigated the involvement of the MAPK, ERK, and the transcription factor, NF-kappaB, in this IL-10-mediated effect. c9, t11-CLA enhanced ERK activation after LPS stimulation, and inhibition of ERK resulted in abrogation of IL-10 and recovery of IL-12 production. c9, t11-CLA decreased NF-kappaBDNA binding after LPS stimulation, which was concomitant with delayed translocation of NF-kappaBp65 into the nucleus and an increase in IkappaBalpha. These effects were reversed by addition of a neutralizing anti-IL-10 Ab. Our findings demonstrate that c9, t11-CLA suppresses IL-12 production by LPS-stimulated DC by ERK mediated IL-10-induction. Furthermore, these IL-10-mediated effects are dependent on inhibition of NF-kappaB activation. This is the first study to demonstrate that c9, t11-CLA can enhance transcription and production of the anti-inflammatory cytokine IL-10, while inhibiting the Th1-promoting cytokine IL-12, and may explain certain of its immunosuppressive properties.
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Células Dendríticas / Adyuvantes Inmunológicos / FN-kappa B / Interleucina-10 / Interleucina-12 / Ácidos Linoleicos Conjugados / Quinasas MAP Reguladas por Señal Extracelular / Inmunosupresores Límite: Animals Idioma: En Revista: J Immunol Año: 2005 Tipo del documento: Article País de afiliación: Irlanda
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Células Dendríticas / Adyuvantes Inmunológicos / FN-kappa B / Interleucina-10 / Interleucina-12 / Ácidos Linoleicos Conjugados / Quinasas MAP Reguladas por Señal Extracelular / Inmunosupresores Límite: Animals Idioma: En Revista: J Immunol Año: 2005 Tipo del documento: Article País de afiliación: Irlanda