Serum concentrations of adiponectin and characterization of adiponectin protein complexes in dogs.
Am J Vet Res
; 68(1): 57-62, 2007 Jan.
Article
en En
| MEDLINE
| ID: mdl-17199419
ABSTRACT
OBJECTIVE:
To assess serum concentrations of adiponectin and characterize adiponectin protein complexes in healthy dogs. ANIMALS 11 healthy dogs. PROCEDURES Sera collected from 10 dogs were evaluated via velocity sedimentation and ultracentrifugation, SDS-PAGE, western immunoblotting, and radioimmunoassay. Visceral adipose tissue (approx 90 g) was collected from the falciform ligament of a healthy dog undergoing elective ovariohysterectomy, and adiponectin gene expression was assessed via a real-time PCR procedure.RESULTS:
Adiponectin gene expression was detected in visceral adipose tissue. Serum adiponectin concentrations ranged from 0.85 to 1.5 microg/mL (mean concentration, 1.22 microg/mL). In canine serum, adiponectin was present as a multimer, consisting of a low-molecular-weight complex (180 kd); as 3 (180-, 90-, and 60-kd) complexes under denaturing conditions; as 2 (90- and 60-kd) complexes under reducing conditions; and as a dimer, a monomer, and globular head region (60, 30, and 28 kd, respectively) under reducing-denaturing conditions. It is likely that adiponectin also circulates as a high-molecular-weight (360- to 540-kd) complex in canine serum, but resolution of this complex was not possible via SDS-PAGE. CONCLUSIONS AND CLINICAL RELEVANCE After exposure to identical experimental conditions, adiponectin protein complexes in canine serum were similar to those detected in human and rodent sera. Circulating adiponectin concentrations in canine serum were slightly lower than concentrations in human serum. Adiponectin gene expression was identified in canine visceral adipose tissue. Results suggest that adiponectin could be used as an early clinical marker for metabolic derangements, including obesity, insulin resistance, and diabetes mellitus in dogs.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Perros
/
Adiponectina
Límite:
Animals
Idioma:
En
Revista:
Am J Vet Res
Año:
2007
Tipo del documento:
Article
País de afiliación:
Estados Unidos