In-vivo confocal real-time mini-microscopy in animal models of human inflammatory and neoplastic diseases.
Endoscopy
; 39(4): 350-6, 2007 Apr.
Article
en En
| MEDLINE
| ID: mdl-17427071
ABSTRACT
BACKGROUND AND STUDY AIMS:
Although various improvements in tissue imaging modalities have recently been achieved, in-vivo molecular and subsurface imaging in the field of gastroenterology remains a technical challenge. In this study we evaluated a newly developed, handheld, miniaturized confocal laser microscopy probe for real-time in-vivo molecular and subsurface imaging in rodent models of human disease. MATERIALS ANDMETHODS:
The minimicroscope uses a 488-nm, single line laser for fluorophore excitation. The optical slice thickness is 7 microm, the lateral resolution 0.7 microm. The range of the z-axis is 0-250 microm below the tissue surface. Imaging was performed using different fluorescent staining protocols; 5-carboxyfluorescein-labeled octreotate was synthesized for targeted molecular imaging.RESULTS:
Cellular and subcellular details of the gastrointestinal tract could be visualized in vivo at high resolution. Confocal real-time microscopy allowed in-vivo identification of tumor vessels and liver metastases, as well as diagnosis of focal hepatic inflammation, necrosis, and associated perfusion anomalies. Somatostatin-receptor targeting permitted in-vivo molecular staining of AR42-J-induced carcinoma and pancreatic islet cells.CONCLUSIONS:
Confocal mini-microscopy allows rapid in-vivo molecular and subsurface imaging of normal and pathological tissue in the gastrointestinal tract at high resolution. Because this technology is applicable to humans, it might impact on future in-vivo microsocpic and molecular diagnosis of diseases such as cancer and inflammation.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Microscopía Confocal
/
Neoplasias Gastrointestinales
/
Inflamación
Tipo de estudio:
Evaluation_studies
/
Guideline
Límite:
Animals
Idioma:
En
Revista:
Endoscopy
Año:
2007
Tipo del documento:
Article
País de afiliación:
Alemania