On the opening of an insensitive cyclosporin A non-specific pore by phenylarsine plus mersalyl.
Cell Biochem Biophys
; 49(2): 84-90, 2007.
Article
en En
| MEDLINE
| ID: mdl-17906363
ABSTRACT
The purpose of this work was addressed to provide new information on the effect of thiol reagents on mitochondrial non-specific pore opening, and its response to cyclosporin A (CSA). To meet this proposal phenylarsine oxide (PHA) and mersalyl were employed as tools to induce permeability transition and CSA to inhibit it. PHA-induced mitochondrial dysfunction, characterized by Ca2+ efflux, swelling, and membrane de-energization, was inhibited by N-ethylmaleimide and CSA. Conversely, mersalyl failed to inhibit the inducing effect of phenylarsine oxide, it rather strengthened it. In addition, the effect of mersalyl was associated with cross-linking of membrane proteins. The content of membrane thiol groups accessible to react with PHA, mersalyl, and PHA plus mersalyl was determined. In all situations, permeability transition was accompanied by a significant decrease in the whole free membrane thiol content. Interestingly, it is also shown that mersalyl hinders the protective effect of cyclosporin A on PHA-induced matrix Ca2+ efflux.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Arsenicales
/
Activación del Canal Iónico
/
Ciclosporina
/
Membranas Mitocondriales
/
Mersalil
Límite:
Animals
Idioma:
En
Revista:
Cell Biochem Biophys
Asunto de la revista:
BIOFISICA
/
BIOQUIMICA
Año:
2007
Tipo del documento:
Article
País de afiliación:
México