Conformational change induced by metal-ion-binding to DNA containing the artificial 1,2,4-triazole nucleoside.
Inorg Chem
; 46(24): 10114-9, 2007 Nov 26.
Article
en En
| MEDLINE
| ID: mdl-17973476
ABSTRACT
A conformational switch can be induced upon the addition of transition-metal ions to oligonucleotides that contain a row of successive artificial nucleobases flanked by complementary sequences of natural nucleobases, provided that the artificial bases cannot undergo self-pairing via hydrogen bonding but only via the formation of metal-ion-mediated base pairs. Such oligonucleotides adopt a hairpin structure in the absence of transition-metal ions, yet they show a preference for the formation of a regular double helix if the appropriate metal ions are present. We report here our experimental data on the structure of the oligonucleotide d(A7X3T7) (A=adenine, T=thymine, X=1,2,4-triazole) in the absence and presence of silver(I). This study comprising temperature-dependent UV spectroscopy, CD spectroscopy, MALDI-TOF measurements, fluorescence spectroscopy, and dynamic light scattering opens up a new approach to the generation of a large variety of metal-ion sensors with the possibility of fine-tuning their sensing capabilities, depending on the artificial nucleoside that is used.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Oligonucleótidos
/
Plata
/
Triazoles
/
ADN
/
Conformación de Ácido Nucleico
/
Nucleósidos
Idioma:
En
Revista:
Inorg Chem
Año:
2007
Tipo del documento:
Article
País de afiliación:
Alemania