Your browser doesn't support javascript.
loading
Conformational change induced by metal-ion-binding to DNA containing the artificial 1,2,4-triazole nucleoside.
Böhme, Dominik; Düpre, Nicole; Megger, Dominik A; Müller, Jens.
Afiliación
  • Böhme D; Department of Chemistry, University of Dortmund, Otto-Hahn-Strasse 6, 44227 Dortmund, Germany.
Inorg Chem ; 46(24): 10114-9, 2007 Nov 26.
Article en En | MEDLINE | ID: mdl-17973476
ABSTRACT
A conformational switch can be induced upon the addition of transition-metal ions to oligonucleotides that contain a row of successive artificial nucleobases flanked by complementary sequences of natural nucleobases, provided that the artificial bases cannot undergo self-pairing via hydrogen bonding but only via the formation of metal-ion-mediated base pairs. Such oligonucleotides adopt a hairpin structure in the absence of transition-metal ions, yet they show a preference for the formation of a regular double helix if the appropriate metal ions are present. We report here our experimental data on the structure of the oligonucleotide d(A7X3T7) (A=adenine, T=thymine, X=1,2,4-triazole) in the absence and presence of silver(I). This study comprising temperature-dependent UV spectroscopy, CD spectroscopy, MALDI-TOF measurements, fluorescence spectroscopy, and dynamic light scattering opens up a new approach to the generation of a large variety of metal-ion sensors with the possibility of fine-tuning their sensing capabilities, depending on the artificial nucleoside that is used.
Asunto(s)
Buscar en Google
Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Oligonucleótidos / Plata / Triazoles / ADN / Conformación de Ácido Nucleico / Nucleósidos Idioma: En Revista: Inorg Chem Año: 2007 Tipo del documento: Article País de afiliación: Alemania
Buscar en Google
Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Oligonucleótidos / Plata / Triazoles / ADN / Conformación de Ácido Nucleico / Nucleósidos Idioma: En Revista: Inorg Chem Año: 2007 Tipo del documento: Article País de afiliación: Alemania