PCR-RFLP assay for species and subspecies differentiation of the Streptococcus bovis group based on groESL sequences.
J Med Microbiol
; 57(Pt 4): 432-438, 2008 Apr.
Article
en En
| MEDLINE
| ID: mdl-18349361
ABSTRACT
The sequence diversity of groESL genes among Streptococcus bovis group isolates was analysed, including five reference strains and 36 clinical isolates. Phylogenetic analysis of the groES and groEL sequences showed that the isolates that belonged to the same species or subspecies usually clustered together. The intergenic spacer region between groES and groEL was variable in size (67-342 bp) and sequence and appeared to be a unique marker for species or subspecies determination. Sequence similarities of the groESL genes among species and subspecies ranged from 84.2 to 99.0 % in groES, and from 88.0 to 99.0 % in groEL. Based on the sequences determined, a Streptococcus bovis group-specific PCR assay was developed, which may provide an alternative means of distinguishing the bovis group from other viridans streptococci. Restriction digestion of the amplicon with AclI further differentiated the species and subspecies.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Polimorfismo de Longitud del Fragmento de Restricción
/
Reacción en Cadena de la Polimerasa
/
Técnicas de Tipificación Bacteriana
/
Streptococcus bovis
/
Chaperoninas
Tipo de estudio:
Evaluation_studies
Límite:
Humans
Idioma:
En
Revista:
J Med Microbiol
Año:
2008
Tipo del documento:
Article
País de afiliación:
Taiwán