[Expression of annexin I in human pancreatic cancer and the influence of its down-regulation on biology of this cancer].
Zhonghua Zhong Liu Za Zhi
; 29(10): 738-41, 2007 Oct.
Article
en Zh
| MEDLINE
| ID: mdl-18396684
ABSTRACT
OBJECTIVE:
To investigate the expression of annexin in human pancreatic cancer and to elucidate its role in oncogenesis of pancreatic cancer.METHODS:
A pancreatic carcinoma cell line Suit-II with high-expression of annexin I gene was adopted. Three subtypes of annexin I -siRNA sequences and a non-related fragment were combined, and the eukaryotic expression vectors bearing siRNA fragments were constructed. Then they were transfected into pancreatic carcinoma cells to knock down the expression of annexin I by RNAi. After knocking down the expression of annexin I , the growth speed, cell cycling, morphological features and apoptosis of pancreatic carcinoma cells were examined by RT-PCR and MTT test.RESULTS:
When the expression of annexin I was blocked, the growth speed of pancreatic carcinoma cells was significantly decreased, the morphological features were changed and pronounced apoptosis occurred.CONCLUSION:
Annexin I can modulate pancreatic carcinoma cell cycle, promote the cell proliferation, increasingly stimulate the cell growth, and suppress the process of apoptosis in pancreatic carcinoma cells.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Neoplasias Pancreáticas
/
Apoptosis
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Anexina A1
/
ARN Interferente Pequeño
Límite:
Humans
Idioma:
Zh
Revista:
Zhonghua Zhong Liu Za Zhi
Año:
2007
Tipo del documento:
Article
País de afiliación:
China