[Proto-oncogene c-src regulates the viability of rat spermatogonial stem cells in vitro through phosphorylated signal transducer and activator of transcription-3].
Sheng Li Xue Bao
; 60(3): 391-6, 2008 Jun 25.
Article
en Zh
| MEDLINE
| ID: mdl-18560731
ABSTRACT
The present study aimed to investigate the effect of proto-oncogene c-src on the viability of rat spermatogonial stem cells from 9 day-old rat in vitro. MTT method was used to observe the viability of the spermatogonial stem cells treated with antisense c-src oligodeoxynucleotides (ODNs) in vitro; RT-PCR was utilized to observe the expression of c-src mRNA and Western blot was used to observe the protein expressions of pp60c-src and phosphorylated signal transducer and activator of transcription-3 (p-STAT3). Compared with that in control group, the viability of spermatogonial stem cells decreased by 8.1% (P<0.05) and the expression of c-src mRNA decreased significantly after treatment with 10 µmol/L antisense c-src ODNs for 12 h. Compared with that in the control group, the protein expressions of pp60c-src and p-STAT3 decreased by 33.8% and 45.3% (both P<0.01), respectively, in the spermatogonial stem cells after being transfected with antisense c-src ODNs. The results suggest that proto-oncogene c-src regulates the viability of rat spermatogonial stem cells through p-STAT3.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Espermatogonias
/
Células Madre
/
Proteínas Proto-Oncogénicas pp60(c-src)
/
Factor de Transcripción STAT3
Límite:
Animals
Idioma:
Zh
Revista:
Sheng Li Xue Bao
Año:
2008
Tipo del documento:
Article
País de afiliación:
China